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Graves 병에서 배양된 백서 갑상선 세포 ( FRTL - 5 ) 를 이용한 갑상선 자극항체의 측정 - 기본적 검토 및 임상적 의의 -
조보연(Bo Youn Cho),송영기(Young Kee Shong),이홍규(Hong Kyu Lee),고창순(Chang Soon Koh),민헌기(Hun Ki Min),이문호(Mun Ho Lee) 대한내과학회 1988 대한내과학회지 Vol.34 No.2
N/A We tried to assess the best conditions to measure thyroid stimulating antibodies (TSAb) using cultured rat thyroid cell ling (FRTL-5), And also to evaluate the frequencies of TSAb in Graves' disease, changes of TSAb activities during antithyroid drug treatment, and whether the presence of TSAb at the end of treatment can predict the relapse, we measured TSAb using FRTL-5 cells in 40 normal controls and 179 patients with autoimmune thyroid diseases. The results are as following; 1) The cAMP responses of FRTL-5 cells to TSH and TSAb began to increase from 3 days after TSH withrawal from culture media and reached peak response at the 7th day, and thereafter decreased. 2) The cAMP responses of FRTL-5 cells to TSH and TSAb increased in relation to incubation times. 3) The dose-response curves of TSH and TSAb, stimulating cAMP production in FRTL-5 cells, showed similary sigmoid curves. The minimum levels of TSH and TSAb which showed significant increase of cAMP were 2-5 μU/ml and 0.15-0.3mg/well, respectively. 4) Intracelluar cAMP levels were correlated closely with extracelluar cAMP levels. About 75-80% of total cAMP levels was released into culture supernatant. 5) The frequencies of detectable TSAb in 57 patients with untreated Graves' disease, in 55 patients with euthyroidism under treatment and in 18 patients with remission were 93%, 56.4% and 11.1%, respectively. However, only 2 out of 21 patients with Hashimoto's thyroditis had low titers of TSAb in their sera. TSAb were not present in sera of all 28 patients with primary nongoitrous myxedema. 6) TSAb activites were singnificantly correlated with TBII activities (r=0.635, p<0.001). 7) In remission group, TSAb activities decresed significantly from 3 months after treatment, and disappeared in alomost all cases within 12 months. However, those activities were not changed significantly untill the end of treatment in relapse group. 8) The positive rate of TSAb in 27 patients who were in remission after treatment was 38.7%, however, that in 28 patients who relapse within 12 months aftr discontinuation of drugs was 77.8%. The predicitve value of TSAb for relapse was 67.7%, which was similar with that of TBII (71.4%). These findings suggest that determination of TSAb using FRTL-5 cells is very sensitive and specific method and that TSAb is one of major cause of hyperthyroidism in Graves disease, and its determination can be useful for the evaluation of clinical course and prognosis in patients with Graves disease.
갑상선 세포의 성장에 대한 TSH 및 IGF - I 의 신호전달 과정에서 백일해 독소 예민성 G - 단백의 역할
조보연(Bo Youn Cho),이동수(Dong Soo Lee),이가희(Ka Hee Yi),송민호(Min Ho Shong),이홍규(Hong Kyu Lee),고창순(Chang Soon Koh),민헌기(Hun Ki Min) 대한내과학회 1991 대한내과학회지 Vol.41 No.1
N/A To evaluate the effect of pertussis toxin sensitive G-proteins on the growth of thyrocytes by TSH and IGF-I, we measured 3H-thymidine incorporation into FRTL-5 cells stimulated by TSH and/or IGF-I. Thymidine incorporation into FRTL-5 cells by TSH(1 mU/ml) or IGF-I (10 ng/ml) was significantly inhibited by the pretreatment of pertussis toxin (25 ug/ml). Adenosine (1mM) inhibited both TSH-and IGF-I- stimulated thymidine incorporation into FRTL-5 cells and the synergistic effect of TSH and IGF-I. This inhibitory effect of adenosine on TSH-or 1GF-I-stimulated thymidine incorporation was blocked partially by the pretreatment of pertussis toxin. However, the inhibition of the synergism of TSH and IGF-I by adenosine was not altered by the pretreatment of pertussis toxin. Indomethacin (100 uM) inhibited TSH effects by 60% and inhibieed IGF-1 effects by 5096 at 24 hours of incubation and then enhanced IGF-I effects in the prolonged incubation. Indomethacin blunted the synergism of TSH and IGF-I. These inhibitory effects of indomethacin were altered by the pretreatment of pertussis toxin. The pretreatment of pertussis toxin did not affect the inhibitory effect of indomethacin on the synergism of TSH and IGF-I at 24 hours of incubation. However, in the prolonged incubation (72 hours) the pretreatment of pertussis toxin significantly blocked the indomethacin effect. These findings suggest that pertussis toxin-sensitive G-proteins are involved in the signal transduction of TSH and/or IGF-1-stimulated growth of FRTL-5 cells and arachidonate metabolites should be involved in the mediation of synergism of growth stimulation induced by TSH and IGF-l in FRTL-5 cells.
조보연 ( Bo-yun Cho ) 한국정보처리학회 2012 한국정보처리학회 학술대회논문집 Vol.19 No.2
인터넷의 활용이 보편화 됨에 따라 정보의 양은 급격히 늘어나고 있다. 이에 취업을 희망하는 구직자의 경우 IR 로부터 원하는 정보를 검색하기 위해 과거보다 더 많은 시간과 노력이 필요하게 되었다. 이에 본 논문에서는 TF(Term Frequency)기법을 통해 문서를 추출하고 추출된 문서의 Doc_ID 빈도수를 기준으로 한 내용기반과 군집기법을 혼합한 하이브리드 검색 시스템을 제안한다. 구직자들이 클릭한 취업정보들의 링크번호들을 K-means 알고리즘을 이용하여 군집화를 한다. 생성된 군집들은 각기 하나의 문서로 가정하고, 기존 문서과 더불어 검색 주제와 연관성을 갖고 있는 문서들을 동적비율로 검색 랭킹 하는 방식이다. 기존의 IR 기술과의 비교 실험을 통해 성능을 평가하였다. 실험결과 본 논문에서 제안한 방법이 기존의 방법보다 우수함을 확인할 수 있었다
배양된 쥐 갑상선세포 성장에 미치는 각종 성장인자들과 TSH 수용체항체간의 상호작용 및 그 기전에 관한 연구
조보연(Bo Youn Cho),송영기(Young Kee Shong),이홍규(Hong Kyu Lee),고창순(Chang Soon Koh),민헌기(Hun Ki Min) 대한내과학회 1989 대한내과학회지 Vol.37 No.3
N/A To evaluate the interaction of various growth factors and TSH receptor antibodies (TRAb) on the growth of cultured rat thyroid cells, FRTL-5 cells, and to verify their mechanisms of action, we measured 3H-thymidine incorporation into FRTL-5 cells with various combinations of TSH, Graves' IgG and other growth stimulators (insulin, IGF-I, multiplication stimulation activity, forskolin, dBcAMP and phorbol ester). We evaluated the effects of blocking TRAb and adenosine on the growth factor-stimulated growth of FRTL-5 cells. Insulin, IGF-I and MSA increased 3H-thymidine incorporation into FRTL-5 cells and showed a synergistic effect when incubated simultaneously with TSH or Graves IgG. Forskolin, PGE2, and dBcAMP also increased 3H-thymidine incorporation by themselves and their effects were synergistically potentiated by insulin and IGF-I. Adenosine inhibited both TSH and Graves' IgG induced 3H-thymidine incorporation into FRTL-5 cells. Blocking TRAb inhibited both TSH and Graves' IgG induced 3H-thymidine incorporation, but did not inhibit the effects of insulin, IGF-I, forskolin and dBcAMP on the growth of FRTL-5 cells. Phorbol ester (TPA) did stimulate the growth of FRTL-5 cells by itself and inhibited both TSH and Graves IgG induced 3H-thymidine incorporation into FRTL-5 cells. These results suggest that 1) there might be two or more signal transduction systems for the growth of thyroid cells; TSH and Graves IgG stimulate the growth of thyroid cells through the adenylate cyclase-cAMP system, and IGF-I and other growth facors act through another system, and 2) blocking TRAb may inhibit the growth of thyroid cells by inhibiting TSH-stimulated cAMP generation.
분화성 갑상선암에서 수술 후 I - 131을 이용한 잔여 갑상선 조직의 제거 성적
조보연(Bo Yeon Cho),고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(Jung Key Chung),이동수(Dong Soo Lee),김유경(Yu Kyeong Kim),정재민(Jae Min Jeong) 대한핵의학회 1997 핵의학 분자영상 Vol.31 No.3
N/A To evaluate the effectiveness of I-131 in ablation of residual thyroid tissue, we analyzed 350 patients with thyroid cancer who were treated with various doses of I-131 after surgery for thyroid cancer. Two hundred fifty five patients were treated with l.lGBq(30mCi) of I-131 for ablation of remnant thyroid and one hundred seventeen patients received more than 2.8GBq(75mCi) of I-131. We determined the effectiveness of ablation by following I-131 whole body scan. Absent visible uptake or minimal uptake in thyroid tissue were considered as successful ablation. Of 255 patients who received doses of 30mCi I-131 therapy, 131 patients(51%) showed successful ablation of residual thyroid tissue with 2.6±1.7 times of I-131 therapy. Of 117 patients who received doses of the more than 75mCi I-131, 84 patients(72%) had successful remnant thyroid ablation with 1.6±1.1 times of I-131 therapy. According to the extent of surgery, successful ablation rates were 78%, 62%, 54%, 33% in patients who underwent total thyroidectomy, subtotal thyroidectomy, lobectomy and isthmectomy, lobectomy or tumorectomy, respectively. This study showed that ablation of remnant thyroid after surgery with 30mCi I-131 was successful only in 50%. Therefore, in cases of patients with high risk for recurrence, we recommend high dose I-131 for ablation of remnant after total thyroidectomy.