서울 월드컵경기장의 상부 막구조물의 제작 및 시공

조병욱,Cho, Byung-Wook 한국공간구조학회 2001 한국공간구조학회지 Vol.1 No.2

Development of Sequence Characterized Amplified Regions (SCAR) Showing for Cheju Native Horse

조병욱,Cho Byung Wook Korean Society of Life Science 2005 생명과학회지 Vol.15 No.3

본 연구는 RAPD 기법을 이용한 종 특이 marker 개발 및 이 marker의 SCAR marker로의 개발을 목표로 수행되었다. Random primer 700개에 대하여 PCR 수행결과, 품종간, 개체간에 많은 다형성이 관찰되었으며 품종특이적인 양상을 나타내는 MG30, MG53의 primer는 각각 2.0kb, 2.3kb의 위치에서 제주말과 더러브렛종의 특이적인 RAPD 단편을 나타내었다. 이들 단편들 중 품종 특이적인 단편을 클로닝한 후 random primer가 포함된 부분의 염기서 열을 결정하였다. 10 bp의 RAPD random primer에 10bp의 염기를 추가하여 SCAR primer를 제작하였다. SCAR marker의 수행결과 RAPD marker와 같은 2.3kb, 2.0kb의 크기에서 제주마와 더러브렛종에 특이적인 하나의 밴드가 증폭되었다. 따라서 이 Cnh-SCAR marker는 보다 안정적이고 재현성 있는 marker로서 사용이 가능하여 제주말의 판별에 유용하게 사용될 수 있을 것이다. This study was conducted to analyze genetic characteristics and to develop the specific marker for Cheju native horse (Coo) at the level of sequence characterized amplified regions (SCARs). We collected blood samples from Cheju native horse and Thoroughbred horse (Th) and obtained genomic DNA from the blood of 50 individuals randomly selected within the breeds. Seven hundred primers were chosen randomly and were used to examin the polymorphism and 40 kinds of primers showed polymorphic RAPD band patterns between two breeds. Thirty primers of them showed horse specific bands. With the primer MG 30, amplified band of 2.0 kb showed the specificity to Cheju native horse (Cnh). Additionally MG 53 detected the thoroughbred horse (Th) specific markers at size of 2.3 kb. As the next, 2.3 kb band from MG 53 was checked with the all individuals from all the breeds of this study, and it maintained the reproducible breed specificity to thoroughbred horse (Th). With this results, 2.3 kb band was cloned into plasmid vector and sequenced bidirectionally from both ends of the cloned fragment. With the obtained sequences 10 nucleotide extended primers including the original arbitray primer were designed as a SCARs primer. Finally, the primer with extended sequence showed the reproducible breed differentiation pattern and it was possible to identify Cheju native horse (Cnh) from other breeds. The SCARs marker 2.3 kb from MG 53 could be used to identify Cheju native horse (Cnh) for not only registration but also horse breeding programe.

일산 웨스턴 돔 지붕

조병욱,Cho, Byung-Wook 한국공간구조학회 2007 한국공간구조학회지 Vol.7 No.2

밀양지방 토종개의 형태학적 특징 및 유전적 다양성 연구

조병욱,조길재,Cho, Byung-Wook,Cho, Gil-Jae 한국생명과학회 2006 생명과학회지 Vol.16 No.4

밀양 토종개의 일반적인 특징을 구명할 수 있는 기초자료를 확보하고자 밀양 토종개 44두를 대상으로 형태학적 특징 및 microsatellite DNA형의 유전적 다양성의 출현빈도에 기초한 유전적인 특징을 조사한 결과 밀양 토종개의 체고는 43-55 cm(평균 49.5 cm)로서 수캐는 44-55 cm(평균 50.3 cm), 암캐는 43-52 cm(평균 48.1 cm)로 나타났고 체장은 45-60 cm(평균 54.3 cm)로서 수캐는 45-60 cm(평균 55.9 cm), 암캐는 45-57 cm(평균 52.6 cm)였다. 또한 가슴둘레는 수캐가 51-64 cm(평균 59.2 cm), 암캐는 50-62 cm(평균 56.3 cm)로 측정되었다. 머리의 형태는 정면에서 보았을 때 44두 모두에서 역삼각형 형태를 가지고 있었으며, 눈의 모양은 삼각형 형태가 40두(90.9%)였고 초승달 모양이 4두(9.1%)로 관찰되었다. 모색은 백색이 41두(93.2%), 황색이 3두(6.8%)로 나타나 두 색깔을 가지고 있었다. 혀와 발톱의 색깔은 전 두수에서 각각 연분홍색과 분홍색이 관찰되었고 항문의 색깔은 연한 흑색이 40두(90.9%), 연분홍색이 4두(9.1%)로 나타났다. 그리고 귀의 형태는 전 두수가삼각형의 곧게 서 있는 형태였으며, 꼬리의 형태는 반말린 꼬리가 25두(56.8%)로 가장 많았고 선꼬리(장대꼬리)가 15두(34.1%), 말린 꼬리가 4두(9.1%)로 나타났다. 15개의 marker로 분석한 microsatellite DNA 다형의 유전자 빈도에 기초하여 heterozygosity, PIC 그리고 PE를 분석한 결과 대립유전자의 수는 $2{\sim}14$개(평균 6.13개)로 검출되었으며 expected heterozygosity와 PIC는 각각 $0.455{\sim}0.863$ (평균 0.635), $0.348{\sim}\;0.837$(평균 0.570)으로 나타났고 PEZ 10, PEZ 13, PEZ 17, FHC 2054의 marker는 PIC 0.7이상으로 관찰되었다. PE 1은 $0.101{\sim}\;0.548$으로서 15개 marker를 조합시 0.9895, PE 2는 $0.174{\sim}\;0.710$으로서 전체 조합시 0.9996으로 나타났다. This study was carried out to investigate the physical characteristics (height, body lenght, chest depth, head type, ear type, body color, eye type and tail type) and genetic diversity using 15 microsatellite DNA markers (PEZ 1, 5, 8, 10, 11, 12, 13, 15, 16, 17, 20, 21, FHC 2010, FHC 2054 and FHC 2079) in 44 random Miryang native dogs(6 months${\sim}$12 years old). The height, body lenght, and chest depth of Miryang native dogs were 43-55 cm(mean 49.5 cm), 45-60 cm(mean 54.3 cm), and 50-64 cm(mean 57.9 cm), respectively. Miryang native dog was medium sized. The head and eye type were reverse-triangle(100%), triangle (90.9%) and newborn moon(9.1%), respectively. Most of body color had white coat color(93.2%), light pink tongue color(100%), light black anal color(90,9%) and pink claw color(100%). The ear type showed erect ear(100%), and half-curled(56.8%), upward(34.1%), curled(9.1%) in tail type, respectively. Number of alleles observed at a single locus ranged from 2 (PEZ 21 and FHC 2010) to 14 (PEZ 13), with average number of alleles per locus of 6.13. The expected heterozygosities of 15 microsatellite loci were estimated based on gene frequencies. The highest expected heterozygosity, 0.863 was estimated in PEZ 13 locus and the lowest, 0.455 in PEZ 21 and FHC 2010 locus. And the mean expected heterozygosity of 15 microsatellite markers was calculated as 0.635. Polymorphic information content (PIC) values were ranged from 0.348 (PEZ 21 and FHC 2010) to 0.837 (PEZ 13), and the mean PIC value was calculated as 0.570. Of the 15 markers, PEZ 10, PEZ 13, PEZ 17 and FHC 2054 loci have relatively high PIC value (> 0.7) in Miryang native dog. In order to determine the efficieney of parentage control, exclusion probabilities (PE) were calculated for each allele. The highest PE 1 and PE 2 in PEZ 13 locus was caculated to 0.548 and 0.710, respectively. And the total exclusion power in PE 1 and PE 2 was calculated to 0.9895 and 0.9996, respectively. These results can give basic information for perservation and research in Miryang native dog, and phylogenetic relationships of the Korean native dog and Asian dog breeds.

새로운 이중공기막구조 개발

조병욱,이혜원,이용기,Cho, Byung-Wook,Lee, Hye-Won,Lee, Yong-Kee 한국공간구조학회 2009 한국공간구조학회지 Vol.9 No.4

수질관리 : 충청남도 금강수계의 수질개선유역 선정을 위한 방법의 적용

조병욱 ( Byung Wook Cho ),김영일 ( Young Il Kim ),김홍수 ( Hong Su Kim ),정우혁 ( Woo Hyeok Jeong ),이상진 ( Sang Jin Yi ),임봉수 ( Bong Su Lim ) 한국물환경학회 ( 구 한국수질보전학회 ) 2009 공동 추계학술발표회 Vol.2009 No.-

21C 통합주제 중심의 중등학교 체육과 교육과정 방향 모색

조병욱(Cho Byung-Wook) 한국체육과학회 2006 한국체육과학회지 Vol.15 No.3

While the official education course at nationwide level went through half the century, the middle school 3rd grade gymnastics classes were reduced I hour a week, and the high school 2nd and 3rd grade gymnastics classes were changed from prerequisites to selectives in the 7th education course, that the school gymnastics are at risk. Although there may be external factors like undesirable educational environments for the secondary school gymnastics education, and social indifference due to other college entrance exam related subjects, the importance of gymnastics subject should be emphasized over them. The future gymnastics education should be focused on the sound use of leisure time, and shattering off of the social pathologies lingering over among human beings. One of the reasons to block the integral personality oriented education was because the three domains of the gymnastics education, such as recognition, rightfulness, and dynamics of emotion have been conducted in separate parallel way. The gymnastics educations have been playing positive roles through easy access to plan, execute, and evaluate, however the results did not tum favorable. Compared to the educations of other advanced countries, the gymnastics education of Korea has been focused on the integration by the subjects and organizing them as the basics. It is highly necessary make efforts to specify the education contents that are focused on the subjects with the development stages in the education courses of the national level, to select the contents that are judged and agreed to be the most important, and to systemize them by the school or by the school grade. Therefore, the gymnastics education courses need be pursued that may build up creative and integral personality oriented education as its direction to move ahead.

이재민을 위한 반영구 주거용 막구조 건축에 관한 연구

조병욱(Cho, Byung Wook),강철희(Kang, Chul Hee) 대한건축학회 2017 대한건축학회 학술발표대회 논문집 Vol.37 No.1

초위성체 DNA를 이용한 제주마 집단의 품종특성 및 개체식별 체계설정

조병욱(Byung Wook Cho),정지혜(Ji Hye Jung),김상욱(Sang Wook Kim),김희수(Heui Soo Kim),이학교(Hak Kyo Lee),조길재(Gil Jae Cho),송기덕(Ki Duk Song) 한국생명과학회 2007 생명과학회지 Vol.17 No.10

본 연구는 서로 다른 상염색체에 위치하고 있는 초위성체유전 표지를 활용하여 제주마 집단의 타 품종과의 차별적 유전특성분석과 제주마 집단에서 활용할 수 있는 효율적인 개체 식별 시스템 설정을 위해 수행되었다. 공시재료로서는 5품종에서 총 191두가 사용되었으며 13종의 좌위에 대한 개체별 유전자형을 분석하였다. 이들 13종에서 출현된 총 대립유전자수는 제주마의 경우 155종이 나타났다. 한국 제주마집단에서 나타난 평균 이형접합도는 0.317-0.902였으며 marker 다형성 정보량은 0.498-0.799로 나타났다. 제주마 집단에서 나타난 대립 유전자 발현 특성은 다른 외래 품종 집단과 매우 상이한 결과를 나타냈다. ATH4 좌위의 경우 제주마 집단에서는 5종의 대립 유전자가 고른 분포를 나타낸 반면 QUA종의 경우와 THO종집단에서 특정 좌위의 극단적 발현 빈도를 나타냈다. 품종특이성을 나타내는 분자표지의 대립유전자 발현양상이 확인되었으며 이러한 품종특이성 발현 분자표지는 집단 내 개체에 대한 품종식별 유전자표지로 활용이 가능한 것으로 나타났다. 9종의 초위성체 유전 표지를 활용할 경우 누적 개체 식별력은 99.9%를 나타냈으며 두마리의 서로 다른 개체가 서로 같은 유전자형을 가질 짝확률은 0.60 × 10<SUP>-10</SUP>으로 추정되었다. 따라서 9종의 선정된 유전표지는 제주마 품종 집단에서 적정 신뢰도를 제공할 수 있는 개체 식별 시스템을 설정할 수 있을 것으로 생각된다. This study was conducted to establish the individual identification system and to estimate the genetic characteristic of Jeju native horse (JNH) using 13 microsatellite markers located on different horse autosomes. The markers were genotyped on 191 animals from five horse breeds including Jeju native horse (JNH). In total, 138 alleles were detected from the genotypes of 13 microsatellite markers. The average heterozygosities ranged from 0.317 to 0.902 and the polymorphic information content (PIC) ranged from 0.498 to 0.799 in JNH. We found that there were significant differences in allele frequencies in JNH when compared with other horse breeds. In ATH4 marker, there were specific allele frequence pattern that some of allele only found in JNH, Mongolian horse (MONG) and Jeju racing horse (JRH). The calculated cumulative power of discrimination (CPD) was 99.9% when nine microsatellite loci were used for analysis in the individual identification system. Also, the matching probability that two unrelated animals would show the same genotypes, was estimated to be 0.60×10<SUP>-10</SUP>. Therefore, in the nine markers used in this study can be used for individual identification in the Jeju native horse population.

하천그룹화 방법을 이용한 수질개선 하천유역의 우선순위 선정

조병욱 ( Byung Wook Cho ),최정호 ( Jeong Ho Choi ),이상진 ( Sang Jin Yi ),김영일 ( Young Il Kim ) 한국물환경학회 2012 한국물환경학회지 Vol.28 No.1

For effective watershed management, it is very important to select the tributaries through selection and concentration of targeted tributary catchments for improving water quality within the limited financial conditions. This study introduced the selection and diagnosis procedure of tributary catchments for improving water quality at Chungcheongnam-do based on water quality and flowrate monitoring, stream grouping method. The tributaries with high value of water quality and flowrate were selected for improving water quality according to stream grouping method. The diagnosis of selected tributaries for improving water quality was performed with analysis of the pollution load (generated, discharged, delivered) and point source discharged pollution load density. The plans for improving water quality of tributaries were suggested thorough various diagnosis of tributary catchments. For improving water quality of tributaries in Chungcheongnam-do, the tributary catchments in the Dangjin, Asan, Yesan, Cheonan, Hongseong area should be preferentially considered. The water quality improving plans for those tributary catchments, in accordance with the reduction of sources of pollution by population and livestock, should be established.