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      • KCI등재

        김치양념과 유기산을 이용한 소형 적색육어 조미숙성제품 개발 1. 꽁치 조미제품의 숙성 중 화학적 변화

        정인학,임영선,JEONG In-Hak,LIM Yeong-Seon 한국수산과학회 2001 한국수산과학회지 Vol.34 No.4

        소형 적색육어의 소비기호에 부정적으로 작용하고 있는 잔뼈를 연화시켜 제거하고 비린내를 저감시키기 위하여 대표적인 소형 적색육어인 꽁치, Cololabis saira를 김치양념과 유기산 그리고 미강을 첨가하여 $5\pm0.5^{\circ}C$에서 60일 동안 숙성시키면서 15일 간격으로 숙성 중 화학적 변화를 검토하였다. 마른간이 물간에 비해 염침투도 및 탈수도가 높아 조미양념과의 평형이 15일 정도 빨랐으며, 그로 인해 숙성 중 pH가 높고, 휘발성염기질소함량과 TBA가가 낮아 조미숙성제품 제조시 염 첨가방법은 마른간이 적합하였다. 마른간에 미강을 첨가한 제품은 첨가하지 않은 제품에 비해 미강에 함유되어 있는 아미노산이 제품으로 이행되어 정미성분의 함량을 나타내는 아미노태질소가 높았고, 단백질 산패억제 및 미강의 carotenoid와 tocopherol 둥의 항산화물질에 의한 지질산화 억제효과도 있어 저이용 자원의 활용 증대측면에서 상업적 제품화가 기대된다. In order to promote the consumption of small red muscle fish, such as saury, sardine, herring, etc., a new conditioning technique to soften backbone and small bones was investigated by using kimchi seasoning ingredients and organic acids. In the conditioning process, various chemical changes were examined during 60 days at 15 days intervals, The decrease of moisture content and the increase of salt concentration in seasoned fish meat were good correlated respectively. In the dry salt conditioning, the moisture content was more rapidly decreased than wet salt conditioning. The pH of fish meat during conditioning were more slowly decreased in dry salting than in wet salting. The VBN contents were suppressed under 30 mg/100 g in dry salt conditioning during 60 days at $5^{\circ}C$. The addition of rice bran in dry salting was effective on retarding lipid oxidation because the TBA value was most effectively retarded.

      • KCI등재

        강릉지방의 오징어 식해 개발에 관한 연구 -1. 숙성온도 및 기간에 따른 성분변화-

        김상무,정인학,조영제,KIM Sang-Moo,JEONG In-Hak,CHO Young-Je 한국수산과학회 1994 한국수산과학회지 Vol.27 No.3

        본 연구는 강릉지역에 향토식품으로만 명맥을 유지하고 있는 오징어 식해를 제조하여 숙성온도 및 숙성기간을 달리하였을 경우의 여러가지 화학적인 변화를 실험하였으며 그 결과를 요약하면 다음과 같다. 숙성기간동안 일반성분 및 염도의 변화는 거의 없었으며, 단지 조단백은 숙성기간이 증가함에 따라 다소 감소하는 경향을 나타내었다. pH는 $5^{\circ}C$를 제외하고는 숙성기간이 증가함에 따라 감소하였으며 특히 $15^{\circ}C$와 $20^{\circ}C$ 숙성에서는 숙성초기부터 급격하게 감소되었으며, 젓산 생성은 pH 감소와 반비례하여 증가하였다. 총 arginine량은 숙성초기에는 다소 증가하는 경향을 보였으나 전반적으로 변화가 거의 없었다. 아미노태 질소량은 숙성 15일까지는 급격하게 증가하다가 그 후로는 서서히 증가하였다. TMAO 량은 $5^{\circ}C$ 및 $10^{\circ}C$에서는 서서히 감소하였으며 $15^{\circ}C$ 및 $20^{\circ}C$에서는 숙성초기에 급격하게 감소하였다. TMA량은 숙성초기에는 $20^{\circ}C$를 제외한 숙성온도에서는 서서히 증가하다가 20일 이후 감소하는 경향을 보였다. 핵산관련물질은 숙성 초기에만 AMP가 존재하였고 Inosine 및 hypoxanthine 이 핵산관련물질의 대부분을 차지하였다. 이상의 결과를 고려할 때 식해숙성 조건은 15일 정도가 최적이며 저온($5^{\circ}C$ 이하)에서는 조금 더 숙성기간이 길어진다고 생각된다. Kang-Nung sik-hae is a traditional Korean fermented fish product which has remained in existence in Kang-Nung district. In order to preserve the traditional culture of Kang-Nung sik-hae and develop it as a commercial product, the property changes of sik-hae were investigated at different fermentation temperatures and periods. The chemical changes during the fermentation of squid(Todarodes pacificus) sik-hae were studied at different fermentation temperatures and periods. The amounts of proximate compositions and salinity did not change significantly at all fermentation temperatures and periods. The value of pH decreased with increasing storage period at all fermentation temperatures except at $5^{\circ}C$, whereas that of lactic acid at all fermentation temperatures increased. The amount of total arginine increased slightly up to 10-day of storage and decreased after that. The amount of $NH_2-N$ increased rapidly up to 15-day of storage at all temperatures and increased slightly after that. The amount of TMAO decreased significantly at $15^{\circ}C\;and\;20^{\circ}C$ and slightly at $5^{\circ}C\;and\;10^{\circ}C$, whereas that of TMA increased significantly at $20^{\circ}C$ up to 5-day of storage and decreased slightly after that. At all experimental temperatures except $5^{\circ}C$, the amount of TMA increased or fluctuated in proportion to the increase in fermentation periods. Inosine and hypoxanthine were the main components of nucleotides and their related compounds during the fermentation of sik-hae. Summarizing the above results, the optimum fermentation period of sik-hae was concluded to be 15-days at all experimental temperatures except $5^{\circ}C$.

      • KCI등재

        적색육어류의 고도불포화지질의 이용에 관한 연구 3. 정제정어리유의 제조

        이강호,정인학,서재수,정우진,육지희,LEE Kang-Ho,JEONG In-Hak,SUH Jae-Soo,JUNG Woo-Jin,RYUK Ji-Hee 한국수산과학회 1988 한국수산과학회지 Vol.21 No.4

        정어리유의 고도불포화지질을 일상식생활을 통하여 효과적으로 이용하기 위하여 정제정어리건의 제조를 위한 탈산, 탈색, 탈취 등의 정제조건을 검토한 결과를 요약하면 다음과 같다. 1. 정어리유의 탄산은 알칼리처리가 효과적이었으며 2M NaOH용액을 $0.5\%$ 과잉첨가하고 $30^{\circ}C$, 30분처리가 적조건이었다. 일반적으로 행하는 인산처리는 탈검에 효과가 없었다. 2. 탈색은 산성백토만을 기름의 $5\%$정도로 첨가하고 감압하에서 $60^{\circ}C$ 20분간 처리하는 것이 가장 효락적이었다. 3. 탈취는 수증기증류가 효과적이었으며 4torr 이하로 감압하면서 $180^{\circ}C$ 이하의 온도에서 하는 것이 바람직하였고 $200^{\circ}C$ 이상에서는 현저한 요오드가의 감소와 지방산조성에 변화를 초래하였다. As the studies on effective utilization of polyunsaturated lipids in sardine (Sardinops melanosticta), the conditions of deacidification, decoloring, and deodorization for processing of refined sardine oil were investigated In the process of refining, phosphoric acid treatment was not effective in removing phosphatides, and optimal condition to neutralize the crude sardine ill was treating for 30min at $40^{\circ}C$. with $0.5\%$ excess of 3M NaOH solution. Decoloring was optimized by adding $5\%$ bleaching earth and treating for 20min at $50^{\circ}C$ under vacuum, and deodorizing was done by steam destillation at $180^{\circ}C$ under 3 torr of vacuum. When deodorizing temperature exceeded $200^{\circ}C$, some changes occured in fatty acid composition and artifacts were appeared on GLC chromatogram.

      • KCI등재

        김치양념과 유기산을 이용한 소형 적색육어 조미숙성제품 개발 2. 꽁치 조미숙성제품의 관능적 품질평가

        임영선,정인학,LIM Yeong-Seon,JEONG In-Hak 한국수산과학회 2001 한국수산과학회지 Vol.34 No.4

        소형 적색육어 중 꽁치, Cololabis saira를 김치와 같은 산성의 조미양념에 담금$\cdot$숙성하여 어육 및 등뼈의 연화와 잔뼈의 감소를 통해 whole 상태로 어린이도 뼈에 대해 아무런 걱정 없이 식용할 수 있도록 기호성이 있는 새로운 가공품을 제조하였다. 마른간 제품에 비해 물간 제품은 숙성 중 신맛, 부패취 및 조직의 과도한 연화로 인해 관능적 품질변화가 나빠졌으며, 이러한 요인들은 조리 후에도 여전히 남아있어 조미숙성제품 제조시 염 첨가방법은 마른간이 적합하였고, 구운 제품이 자숙한 제품에 비해 육즙의 유출량이 적은 관계로 모든 항목에서 관능적 기호도가 높아 조리법은 구이법이 적합하였다. 숙성 60일 후 물간 제품의 주요 유리아미노산은 histidine, leucine, glutamic acid, alanine, valine 등으로 나타났으며, 미강 첨가유무에 관계없이 마른간 제품에서는 histidine, glutamic acid, arginine, leucine, alanine 등으로 나타나 염첨가방법에 따른 유리아미노산 조성의 차이를 보였다. 마른간을 하고 미강을 첨가한 제품은 첨가하지 않은 제품에 비해 색택, 맛, 조직감이 향상되어 관능적 기호도가 가장 높았다. 유리아미노산 총량, 정미성 아미노산 함량, 잔뼈의 감소 및 등뼈 강도의 연화 개선 능도 첨가하지 않은 제품보다 높아 조미숙성제품 제조시 마른 간을 하고 미강을 첨가하는 것이 저이용 자원의 활용 측면에서 상업적 제품화가 기대된다. In odor to improve preference to small red muscle fish such as saury, sardine, herring, etc., a new seasoning and conditioning process was experimented using kimchi seasoning ingredients and organic acids. The sensory profiles during 60 days of conditioning at $5^{\circ}C$ showed better results in dry salting than in wet salting. The additions of rice bran in dry salting gave advantageous effect on the sensory evaluation of conditioned saury with kimchi seasoning. The counts of remained fine bones and hardness of backbones after 60 days of conditioning were about $50\%\;and\;23\%$ in wet salted product, and $38\sim41\%$ and $11\sim14\%$ in dry salted product respectively, as compared to raw saury, The decomposition of protein to amino acids was more severe in wet salted product than in dry salted product. Histidine, leucine, glutamic acid, alanine, and valine in order were abundant in wet salted product, but histidine, glutamic acid, arginine, leucine, and alanine in order were contained plentifully in dry salted product.

      • KCI등재

        적색육어류의 고도불포화지질의 이용에 관한 연구 5. 정제정어리유의 어육연제품중의 첨가및 저장중의 고도불포화지방산의 안정성

        이강호,정인학,서재수,유병진,육지희,LEE Kang-Ho,JEONG In-Hak,SUH Jae-Soo,YOU Byeong-Jin,RYUK Ji-Hee 한국수산과학회 1988 한국수산과학회지 Vol.21 No.4

        As a part of the studies on effective utilization of polyunsaturated lipids in sardine (Sardinops melanosticta) when the refined sardine oil was added to surimi-based product as a dietary supplement of biologically active fatty acids, eicosapentaenoic aicd and docosahexaenoic acid, storage stability and the effect to the qualify of the product was tested. Addition of the refined sardine oil up to $5\%$ to surimi did not affect the textural properties of the product. And the polyunsaturated fatty acids of the sardine oil was fairly stabilized when it stored for 1 month at room temperature and 43 days at $5^{\circ}C$. These results suggested the possibility that the refined sardine oil or other fish oils containing highly polyunsaturated fatty acids, especially EPA and DHA could be used as a food ingredient for dietary supply of the lipids.

      • KCI등재

        적색육어류의 고도불포화지질의 이용에 관한 연구 4. 정제정어리유의 식용유중의 첨가 및 저장중의 고도불포화지방산의 안정성

        이강호,정인학,서재수,정우진,유병진,LEE Kang-Ho,JEONG In-Hak,SUH Jae-Soo,JUNG Woo-Jin,YOU Byeong Jin 한국수산과학회 1988 한국수산과학회지 Vol.21 No.4

        As a part of the studies on effective utilization of polyunsaturated lipids in sardine (Sardinops melanosticta), when the refined sardine oil was mixed with soybean and other vegetable oils storage stability and the effect to the quality of the product was investigated. Addition of 1 volume of refined sardine oil to 4 volumes of soybean oil was recommended to yield 3 in P/S ratio and 1.5g of eicosapentaenoic and docosahexaenoic acid per 40g of the mixed oil for a good storage stability and as a dietary source of EPA requirement for atherosclerotic disease. When the unpurified vegetable oils, sesame oil and perilla oil, were mixed with the same volume of refined sardine oil the content of n-3 fatty acids was increased to $13.36\%\;and\;30.65\%\;%\;from\;0.27\%$ in sesame oil and $29.72\%$ in perilla oil. The n-3/n-6 ratios were also raised to 0.476 and 1.433 from 0.006 and 0.876. And these mixed oils were more stabilized than the refined sardine oil during storage at $30^{\circ}C$.

      • KCI등재

        적색육어류의 고도불포화지질의 이용에 관한 연구 1. 정어리${\cdot}$고등어의 부위별 지질함량 및 지방산 조성의 계절적 변화

        이강호,이병호,정인학,서재수,정우진,김충곤,LEE Kang-Ho,LEE Byeong-Ho,JEONG In-Hak,SUH Jae-Soo,JUNG Woo-Jin,KIM Chung-Gon 한국수산과학회 1986 한국수산과학회지 Vol.19 No.5

        As the first part of the studies on utilization of polyunsaturated lipids in red muscled fishes like sardine (Sardinops melanosticta) and mackerel (Scomber japonicus), lipid distribution in body sections, whole body, meat, viscera, skin, and head, lipids, polar and nonpolar, and fatty acid composition of the oils obtained from these body sections, and seasonal variations in fatty acid composition, particularly in the content of polyenoic acids, EPA and DHA, were determined, The content of total lipids was ranged $22.2\%\;to\;27.2\%$ in case of sardine without broad difference between body sections, while in case of mackerel $36.7\%\;to\;38.8\%$ unevenly in skin and head. The lipids of both fish were composed of more than $80\%$ of non-polar lipid and there was not much differences in the fatty acid composition between the lipids, polar and non-polar. The major fatty acids of the lipids were $C_{14:0},\;C_{16:0},\;C_{18:0},\;C_{16:1},\;C_{18:1},\;C_{20:1},\;C_{20:4},\;C_{20:5},\;and\;C_{22:6}$, and these acids, $C_{18:1},\;C_{16:0},\;C_{22:6},\;C_{18:0},\;C_{20:5},\;C_{14:0},\;C_{16:1}$, were in high quantity in order. Seasonal variation in fatty acid composition of sardine oil did not show any great variety between sections. There was a tendency in general that saturated and monoenoic acids began to decrease in winter and showed the minimum in April; hereafter get to increase again, while polyenoic acids showed the maximum in April. Both $C_{20:5}\;and\;C_{22:6}$ acids were high in the season of April to July. Variation in fatty acid content was in a width of $8\%$ for saturated, $5\%$ for monoenoic, and $12\%$ for polyenoic acid. $C_{20:5}\;and\;C_{22:6}$ acid were varied in $9.4\%\;and\;9.8\%$, respectively. In case of mackerel oil, seasonal variation tended same as in sardine oil except the fact that the saturated and monoenoic acid decreased to the minimum in the term of April to July in which the polyenoic acid content was also higher marking $27.3\%\;to\;36.1\%$ in average. Fatty acid variation was in a range of $6.3\%$ for saturated, $8.3\%$ for monoenoic, and $13.8\%$ for polyenoic acid but variation of $C_{20:5}\;and\;C_{22:6}$ acid was 4, $3\%$ and 3, $4\%$ respectively. When fresh sardine was stored for 10 days at $5^{\circ}C$, oxidation was rapidly initialed and consequently resulted in fast loss of $C_{20:5}\;and\;C_{22:6}$ acids. It is concluded from the results that the catch of sardine and mackerel in the season of April to June would be adequate for the preparation of polyunsaturated lipid condenced oil in regard to sectional lipid distribution and its variation in seasons.

      • KCI등재

        적색육어류의 고도불포화지질의 이용에 관한 연구 2. 정어리유의 고도불포화지질의 농축${\cdot}$정제 및 저장 안정성

        이강호,이병호,정인학,서재수,최병대,송승호,LEE Kang-Ho,LEE Byeong-Ho,JEONG In-Hak,SUH Jae-Soo,CHOI Byeong-Dae,SONG Sung-Ho 한국수산과학회 1986 한국수산과학회지 Vol.19 No.5

        As the second part of the studies on the utilization of polyunsaturated lipids in sardine oil as nutritional or medical supplement, the conditions of lipid extration and concentration, refining, and storage stability of EPA-condensed sardine oil were investigated. In extraction of lipids, solvent ratios of chloroform-methanol mixture(2:1 v/v) affected the final content of unsaturated lipid in extracted oil and recovery. Stepwise solvent fractionation method at various low temperatures was effective to concentrate polyenoic acids like EPA and DHA when acetone or acetone-methanol mixture, added in the ratio of 1:5 (v/v) was applied step by step to different temperatures at 0 to $-35^{\circ}C$. Addition of 1 to $5\%$ (v/v) of water to acetone was also benefit to raise EPA content but that resulted in reducing the yield of condensed oil from $65\%\;to\;28\%$. Concentration rate of polyenoic acids by solvent fractionation in lipid-actone solution (1:5, v/v) at 0 to $-30^{\circ}C$ seemed limited to $5{\sim}8\%$ in fatty acid composition depending on the initial content of those polyenoic acids in the sardine oil. During the extraction, concentration, and alkaline treatment, oxidation was rapidly induced but oxidation products could be thoroughly removed on the process of deceleration and peroxide elimination. To stabilize the reactive polyenoic acid condensed oil during the storage, stuffing nitrogen gas was essential to expel dissolved oxygen in oil or to seal the oil from open air, and the addition of antioxidative agents as BHA and tocopherols were greatly helpful to extend the storage life.

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