The Effect of n-Alkanols on the Lateral Diffusion of Synaptosomal Plasma Membrane Vesicles Isolated from Bovine Cerebral Cortex

정인교,강정숙,윤일,Chung, In-Kyo,Kang, Jung-Sook,Yun, Il The Korean Society of Pharmacology 1993 대한약리학잡지 Vol.29 No.1

Intramolecular excimer formation with the fluorescent probe 1,3-di(1-pyrenyl)propane (Py-3-Py) was used to investigate the effects of methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, 1-hexanol, 1-heptanol, 1-octanol, 1-nonanol and 1-decanol on the lateral diffusion of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex (SPMV). The n-alkanols increased the excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py in the SPMV. In a dose-dependent manner, n-alkanols increased lateral diffusion of hydrocarbon region of bulk (inner+outer monolayers) SPMV lipid bilayers, and the potencies of n-alkanols up to l-nonanol increased with carbon chain length. It appears that the potencies in bilayer fluidization due to the lateral diffusion increase by 1 order of magnitude as the carbon chain length increases by two carbon atoms. The cut-off phenomenon was reached at 1-decanol, where further increase in hydrocarbon length resulted in a decrease in pharmacological activity. n-Alkanols의 분자적 약리작용기전 탐구에 기초자료를 제공하기 위하여 소의 신선한 대뇌피질로부터 분리한 synaptosomal plasma membrane vesicles (SPMV) 지질 이중층의 측방확산운동에 미치는 n-alkanols의 영향을 형광 probe법으로 검색하였다. n-Alkanols는 SPMV 지질 이중층의 측방확산운동 범위와 속도를 농도 의존적으로 증가시켰고 1-nonanol까지는 탄소수가 두개 증가될 때마다 그 효력은 약 10배 가량 증가되었으나 탄소수 10개인 1-decanol의 효력은 오히려 감소되는 경향을 나타내었다.

악안면 영역의 Castleman's disease의 치험례

정인교,김욱규,신상훈,박혜련,이의훈,장원석,Chung, In-Kyo,Kim, Uk-Kyu,Shin, Sang-Hoon,Park, Hye-Ryun,Lee, Eui-Hoon,Jang, Won-Seok 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.5

본 교실에서는 우측 악하부와 우측 안와 하부의 종창으로 내원한 53세 여자 환자에서 Fine Needle Aspiration Biopsy로 림프절의 반응성 과증식으로 진단되어 다시 절제 생검을 시행하여 multi-centric Castleman's disease로 진단하였다. 조직 검사상 유리질 혈관형과 형질세포형이 공존하는 복합형으로 나타났으며 병소의 적출술만을 시행한 후 1년이 지난 지금까지는 별다른 소견없이 좋은 경과를 나타내고 있으며 앞으로도 계속 주의깊은 관찰이 필요로 한다. Castleman's disease or angiofollicular lymph node hyperplasia, is a rare lymphoproliferative disorder. It may be appear as a local or generalized tumor-like condition, usually in chest or abdomen and may involve both lymph nodes and non-nodal tissues. Castleman's disease is an unusal entity which may at times mimic malignancy but is entirely benign in nature. It is topical as it has been noted to occur with AIDS and Kaposi's sarcoma. Careful interpretation of radiogram may help to distinguish Castleman's disease from other tumor condition, such as lymphoma, neurogenic tumor, or even angiofibroma, etc. But exact diagnosis must be made on the basis of histologic confirmation. In addition to histologic features, clinical distinction between the localized and multicentric form is important in selecting appropriate management. Surgical excision of an localized mass is the first choice of treatment. Partial resection, radiotherapy or observation alone may avoid the need for exessively aggressive therapy. Patients with multicentric disease don't benefit by surgical management because of systemic manifestation and rapid deterioration. Thus, antineoplastic agents and steroids may offer an alternative form of therapy. We report a case of female patient with Castleman's disease in oral and maxillofacial region, treated by surgical excision with good results.

부산시 일원의 토양 오염도 조사

정인교,홍성수,윤일,Chung, In-Kyo,Hong, Seong-Soo,Yun, Il 대한약학회 1994 약학회지 Vol.38 No.6

To provide a basis for setting up an environmental pollution policy of Pusan, an investigation of metal contents in the soil of Pusan was carried out from May, 1993 to April, 1994. Soil was sampled from 10 sites of industrial area,8 sites of commercial area, 8 sites of residential area, 8 sites of green area and 8 sites of agricultural area. The industrial area was the most heavily polluted and the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.580, 19.377, 67.348, 59.638, 58.555 and 1.006 ppm, respectively. In the soil of commercial area, the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.453, 19.110, 63.384, 56.006, 37.466 and 0.578 ppm, respectively, and the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.289, 10.312, 55.246, 55.536, 17.695 and 0.610 ppm, respectively, in the soil of residential area. The green area was the least polluted and the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.215, 5.949, 19.366, 37.244, 6.856 and 0.295 ppm, respectively. In the soil of agricultural area, the average contents of Cd, Cu, Zn, Mn, Pb and As were 0.160, 7.077, 25.365, 51.485, 10.607 and 0.499 ppm, respectively The fact that the Cd content of agricultural area was lower than that of green area is remarkable.

위선종의 (胃腺腫) 임상적 (臨床的) 관찰

정을순(Eul Soon Chung),이상종(Sang Jong Lee),이만호(Man Ho Lee),오동주(Dong Joo Oh),이성훈(Sung Hoon Lee),김명숙(Myung Sook Kim),정인교(In Kyo Chung) 대한소화기학회 1986 대한소화기학회지 Vol.18 No.2

Gastric adenoma is a benign polypoid growth called borderline epithelial lesion, atypical epithelial lesion or flat adenoma. Some authors suggest that it may be a precancerous lesion and its frequent association with gastric carcinoma raises a possibility of its malignant potentiality although unclarified. In this context, we assessed 15 cases with gastric adenoma who visited Koryo General Hospital from 1981 to 1986 and obtained following results. 1) The most patients were in the seventh decade followed by the sixth decade, mean age being 61. 9. The ratio of male to female was 1:1.1. 2) The common clinical symptoms with which patients presented were indigestion (80.0%), epigastric pain and loss of appetite. Epigastric tenderness was observed in 40% of patients on physical examination. 3) UGI series taken in 9 patients showed findings compatible with malignant tumor in 3 cases, peptic ulcer in 3 cases and normal finding in 3 cases. 4) Gastroscopic findings of adenomas were pedunculated polypoid mass in 4 cases, elevated lesion in 2 cases, and multiple small nodules in 1 case. In remaining 8 cases, only nonspecific findings such as superficial ulcers or chronic inflammation were observed. 5) In most cases (13 of 15 cases), gastric adenoma was located in the pyloric antrum as a single in numher. 6) Histologically, only one case was associated adenocarcinoma and two cases showed cellular atypism suspected of malignant change although there was no evidence of malignant cell. 7) 12 cases showed symptomatic improvement with medical treatment and 3 cases who were suspected or confirmed as malignancy by endoscopic biopsy took surgery,

직관주의적 유형론에서의 진리개념

정인교 ( In Kyo Chung ) 한국논리학회 2013 論理硏究 Vol.16 No.3

이 글의 목표는 직관주의적 유형론에서의 진리개념이 어떻게 이해될 수 있는지를 검토하고, 이에 의거하여 직관주의적이면서 객관적인 진리개념을 확보하는 문제에 있어서 프라위츠의 논증보다 진전된 논증을 제시하는 것이다. 이를 위해, 직관주의적 유형론에서의 명제, 유형 및 판단의 구분을 프레게의 판단이론과 비교하며 간략히 설명한 후, 직관주의적 유형론에서의 진리판단을 분석하고, 이에 의거해 증명의 존재의 확정성이 어떻게 이해될 수 있는지 밝힐 것이다. 또한 직관주의적 유형론에서 진리판단으로서의 존재판단이 어떻게 이해되어야 하는지, 특히 왜 그것이 존재양화명제가 될 수 없는지 그 이유를 밝히고, 존재판단을 생략적 판단으로 해석하는 한 견해를 비판할 것이다. 직관주의적 유형론에서의 진리개념에 관한 이 글에서의 분석은 증명의 존재의 확정성 문제와 증명의 존재의 비명제적 성격을 분명히 한 점에서 직관주의적이고 객관적인 진리개념에 대한 프라위츠의 규정보다 진전된 형태의 설명임을 밝히고, 이런 진리개념에 대한 주관적 진리개념의 옹호자들의 한 비판에 답할 것이다. I examine the notion of truth in the intuitionistic type theory and provide a better explanation of the objective intuitionistic conception of mathematical truth than that of Dag Prawitz. After a brief explanation of the distinction among proposition, type and judgement in comparison with Frege`s theory of judgement, I examine the judgements of the form ``A true`` in the intuitionistic type theory and explain how the determinacy of the existence of proofs can be understood intuitionistically. I also examine how the existential judgements of the form ``Pf(A) exists`` should be understood. In particular, I diagnose the reason why such existential judgements do not have propositional contents. I criticize an understanding of the existential judgements as elliptical judgements. I argue that, at least in two respects, the notion of truth explained in this paper is a more advanced version of the objective intuitionistic conception of mathematical truth than that provided by Prawitz. I briefly consider a subjectivist`s objection to the conception of truth explained in this paper and provide an answer to it.

논문 : 직관주의적 유형론에서의 명제와 판단

정인교 ( In Kyo Chung ) 한국논리학회 2011 論理硏究 Vol.14 No.2

마틴뢰프의 직관주의적 유형론의 중요 사항들을 설명하고, 그 체계의 가장 중요한 특성 중의 하나인 명제와 판단의 구분에 관해 검토한다. 1절에서 문제를 도입한 후, 2절에서 직관주의적 유형론의 명제개념은 직관주의적 명제개념의 발전된 형태임을 보이고, 3절에서는 직관주의적 유형론에서 가장 기본적인 판단개념을 설명한 후, 4절에서 직관주의적 유형론의 기본적인 추론규칙들을 설명하고 그 적용의 한 사례를 검토할 것이다. 마지막으로 5절에서, 직관주의적 유형론에서 명제와 판단의 구분이 차지하는 중요성을 부연한 후, 기초론적 체계에서 명제와 판단의 구분이 필수적인지의 문제와 관련하여, 통상적인 프레게적 구분으로부터 시작하여 직관주의적 유형론에서와 같은 구분에 이르기 위해서는 어떤 것들이 전제되거나 정당화되어야 하는지 검토할 것이다. We explain some basic elements of Martin-Lof`s type theory and examine the distinction between propositions and judgments. In section 1, we introduce the problem. In section 2, we explain the concept of proposition in the intuitionistic type theory as a development of the intuitionistic conception of proposition. In section 3, we explain the concept of judgment in the intuitionistic type theory. In section 4, we explain some basic inference rules and examine a particular derivation in the theory. In section 5, we examine one route from the Fregean distinction between propositions and judgments to the distinction between them in the intuitionistic type theory, paying attention to the alleged necessity for introducing different forms of judgments.

Lipopolysaccharide로 자극시킨 방사선 조사 치은 섬유아 세포에서 granulocytemacrophage colony-stimulating factor와 transforming growth factor-β1 생성

김홍식(Hong-Sik Kim),이성근(Seong-Geun Lee),김광혁(Kwang-Hyuk Kim),김욱규(Uk-Kyu Kim),김종렬(Jong-Ryoul Kim),정인교(In-Kyo Chung),양동규(Dong-Kyu Yang) 대한구강악안면외과학회 2002 대한구강악안면외과학회지 Vol.28 No.3

Purpose: Irradiation in the oral cancer patients causes early and late complications such as intraoral mucositis and fibrosis, with a various expression of GM-CSF and TGF-β. The purpose of this study was to investigate the production of GM-CSF and TGF-β1 by the irradiated human gingival fibroblasts cultivated with lipopolysaccharide. Materials and Methods: Irradiated (total dose, 60 Gy) human gingival fibroblasts were incubated with LPS. Culture supernatants that were collected at 24, 48, and 72 hours were assessed for GM-CSF and TGF-β1 by enzyme-linked immunosorbent assay. Results: 1. GM-CSF production in nomal gingival fibroblasts was increased with incubation time, but decreased with incubation time in irradiated gingival fibroblasts. GM-CSF production in both normal and irradiated gingival fibroblasts induced with LPS was higher than the control. 2. TGF-β1 production in normal gingival fibroblasts was decreased after 24 hours, but, it was increased until 48 hours in irradiated gingival fibroblasts. TGF-β1 production in normal gingival fibroblasts exposed with LPS was higher than the control. Conversely, It was lower than the control in irradiated gingival fibroblasts exposed with LPS. Conclusion: This indicates that irradiation in gingival fibroblasts may play an important role in radiation-induced intraoral mucositis and fibrosis. However, LPS decreases the production of TGF-β1 in the irradiated gingival fibroblasts.

2 형 당뇨병환자에 있어서의 뇨중 Microalbumin 및 β2 - Microglobulin 배설에 대한 연구

홍기영(Ki Young Hong),정인교(In Kyo Chung),오동주(Dong Ju Oh),이만호(Man Ho Lee),정을순(Eul Soon Chung),이상종(Sang Jong Lee) 대한내과학회 1987 대한내과학회지 Vol.32 No.4

N/A Slightly increased urinary excretion of albumin has been shown to be predictive of clinical nephropathy in insulin dependent diabetes. Such an increase in albumin excretion is not generally measurable by the test ordinarily used to detect proteinuria and thus been termed microalbuminuria. β2-microglobulin is used as an index of tubular reab-sorptive capacity. Simultaneous urinary assay of albumin and β2-microglobulin distinguishes between increased transglomerular albumin escpae and imparied tubular reabsorptive capacity as the cause of increased uinary excretion rates of albumin. To study the markers of renal glomerular and tubular function of the diabetic patients, we initially determined by radioimmunoassay technics, urinary excretion rates of microalbumin and β2-microglobulin in 60 normal subjects and 82 type II clinically nonproteinuric diabetic patients. Twenty-four-hour collection of urine was obtained from 60 normal subjects (31 males and 29 females), ranging in age from 20 to 77 years (mean 48.7), and from 82 patients with type II diabetes (48 males and 34 females), ranging in age from 24 to HO years (mean 54.5) respectively. Urinary excretion of microalbumin ranged from 0.78 to 25.0mgjL (8.00±2.42) in the normal subjects, whereas it was from 0.48 to 86.80 mg/L (17.80±13.0) in the diabetic patients. In the case of urinary excretion of β2-microglabulin, it ranged from 1.2 to 300.0 μg/ L (163.8±48,7) in the normal subjects, and 4.4 to 338.7 p g/L (177.3±62.4) in the diabetes. Mean urinary microablumin excretion was significantly elevated in the diabetics, but β2-microglobulin excretion rates were not different from those of normal controls, suggesting that the increased microalbumin excretion was due to increased transglomerular loss of albumin.

급성 (急性) 바이러스성 간염에서 (肝炎) 혈청 (血淸 ) HBsAg / IgM 결합체의 (結合體) 임상적 (臨床的) 의의

정을순(Eul Soon Chung),이상종(Sang Jong Lee),이만호(Man Ho Lee),정인교(In Kyo Chung),김병호(Byoung Ho Kim),전우규(Woo Kyu Jeon),이승세(Seung Sei Lee) 대한소화기학회 1987 대한소화기학회지 Vol.19 No.1

N/A Circulating immune complexes are frequently found in the blood of patients with acute or chronic hepatitis type B. Recently, Palla and coworkers reported the apparent presence of HBsAg/IgMspecific anti-HBs complexes in acute and chronic hepatitis B and indicated that the presence of these immune complexes may function as prognostic indicators of chronic evolution. Therefore, we observed for the detection of HBsAg/IgM complexes and studied the significance of their presence in acute and chronic type B hepatitis. A total of 155 HBsAg-positive patients were carried out for this study from Mar, 1980 to Feb. 1986 in this hospital. They were included as 28 patients with asymptomatic hepatitis B carriers, 77 patients with acute hepatitis B, and 50 patients with chronic hepatitis B, respectively. The following results in this study were obtained: 1) Circulating HBsAg/IgM complexes were found in 15 out of 28 (54%) asymptomatic hepatitis B carriers, in 26 out of 77 (34%) acute hepatitis B patients, and in 26 out of 50 (52%) chronic hepatitis B patients, respectively. 2) Circulating HBsAg/IgM Complexes in patients with acute B hepatitis were either absent from the initial hospital day or disappeared from four weeks hospitalization, and recovered clinically within two months. 3) The acute hepatitis B patients, being detectable circulating HBsAg/IgM complexes, had persistent elevation of serum alanine transaminase at least six months period. It is suggested that circulating HBsAg/IgM complexes seem to be of important prognostic and diagnostic value in acute and chronic hepatitis B.

Staphylococcus enterotoxin B와 lipopolysaccharide를 작용시킨 사람 섬유아 세포에서 생성된 Transforming Growth $Factor-{\beta}_1$의 정량적 분석

이성근,김광혁,김욱규,김종렬,정인교,양동규,Lee, Seong-Geun,Kim, Kwang-Hyuk,Kim, Uk-Kyu,Kim, Jong-Ryoul,Chung, In-Kyo,Yang, Dong-Kyu 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

$TGF-{\beta}_1$ is a potent chemotactic factor for inflammatory cells and fibroblasts. It also stimulates the celluar source and components of extracellular matrix and the production of proteinase inhibitors. Collectively, these biologic activities lead to the accumulation and stabilization of the nascent matrix, which is vital to infection control. The objective of this study is to investigate production of $TGF-{\beta}$ in vitro fibroblast culture in the presence of Staphylococcus enterotoxin B(SEB) and/or lipopolysaccharide(LPS) and to elucidate the role of $TGF-{\beta}_1$ which may be responsible for infection control. The fibroblasts were originated from gingiva and facial dermis in 26 year-old male patient. In the presence of LPS($0.01{\mu}g$, $0.1{\mu}g$, $1.0{\mu}g$), SEB($0.01{\mu}g$, $0.l{\mu}g$, $1.0{\mu}g$) respectively, $cells(5{\times}10^3ml)$ were cultivated in vitro. At 1, 3, and 5 days after incubation, cells were counted. Also, $cells(2.5{\times}10^5ml)$ were cultivated in EMEM with LPS(0.01, 0.1 and $1.0{\mu}g$), SEB(0.01, 0.1 and $1.0{\mu}g$) respectively and $LPS(0.1{\mu}g)$ and $SEB(0.1{\mu}g)$ in combination for 24, 48, and 72 hours respectively. Culture supernatants were harvested at 1, 2, and 3 days after incubation period and triplicate culture supernatants were pooled and $TGF-{\beta}_1$ was assayed in duplicate. The results were as follows. 1. In gingival fibroblast induced with SEB and LPS respectively or in combination, the suppression of cell Proliferation occurred very significantly since 3 days after incubation, compared with the control and the production of $TGF-{\beta}_1$ occurred very significantly at 1 day after incubation, compared with the control. 2. In facial dermal fibroblast induced with SEB and LPS respectively or in combination, the suppression of cell proliferation occurred very significantly at 1 day after incubation, compared with the control. In SEB exposure, the production of $TGF-{\beta}_1$ was decreased very significantly at 1 day after incubation, compared with the control. However, in LPS, SEB and LPS exposure, the production of $TGF-{\beta}_1$ was increased very significantly at 1 day after incubation, compared with the control. In conclusion, the concentration of bacterial toxins and the incubation period correlated with cell proliferation and production of $TGF-{\beta}_1$ very significantly. The gingival and facial dermal fibroblasts have different phenotype each other The orchestrated understanding of fibroblast proliferation and $TGF-{\beta}_1$ production play an important part in host defense against the bacterial Infection and may prevent tissue necrosis such as necrotizing fasciitis and life-threatening syndrome such as multiple organ failure.