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TOXIC MECHANISM OF Ro09-0198 ISOLATED FROM STREPTOVERTICILLIUM
정세영,Choung, Se-Young The Korean Society of Toxicology Korea Environment 1990 Toxicological Research Vol.6 No.1
Ro09-0198, a cyclic peptide isolated from culture filtrates of Streptoverticillium griseoverticillatum, induced lysis of erythrocytes. Ro-09-0198-induced hemolysis was temperature-dependent and the sensitivity of hemolysis differed greatly among animal species. Preincubation of the peptide with phosphatidylethanolamine reduced the hemolytic activity, whereas other phospholipids present in erythrocytes in nature had no effect. A study of the structural requirements on phosphatidylethanolamine necessary for interaction with the peptide indicates that Ro09-0198 recognizes strictly a particular chemical structure of phosphatidylethanolamine: dialkylphosphoethanolamine as well as 1-acylglycerophosphoethanolamine showed the same inhibitory effct on hemolysis induced by Ro09-0198 as diacylphosphatidyl-ethanolamine, whereas phosphoethanolamine gave no inhibitory effect. Neither phosphatidyl-N-monomethylethanolamine nor alkylphosphopropanolamine had an inhibitory effect. Proton resonances of the peptide were observed in dimethyl sulfoxide solution in the presence of 1-dodecanoyl-sn-glycerophosphoethanolamine. This peptide caused permeability increase and aggregation of liposomes containing phosphatidylethanolamine. A glycerol backbone and a primary amino group of phosphatidylethanolamine are necessary for interaction with Ro09-0198 to cause membrane damage. Ro09-0198 induced a selective permeability change on liposomes. Glucose and umbelliferyl phosphate were effluxed significantly, but sucrose was only slightly permeable and inulin could not be released. Platelet aggregation and serotonin release simultaneously induced by Ro09-0198. Addition of peptide to rat platelet, loaded with the fluorescent $Ca^{++}$ chelator quin-2, caused immediate rise in cytosolic free $Ca^{++}$ to liposomal membrane containing phosphatidylethanolamine was observed dose dependently.
Cyclic Peptide, Ro09-0198의 혈소판활성화에 대한 작용기전
정세영(Se Young Choung) 대한약학회 1991 약학회지 Vol.35 No.1
RoO9-0198, a cyclic peptide isolated from culture filtrates of Streptoverticillium griseoverticillatum, induced platelet aggregation and serotonin release simultaneously. LDH release was not observed. Addition of peptide to rat platelet, loaded with Ca2+ chelator quin-2, caused immediate rise in cytosolic free Ca2+. Liposomal membrane containing phosphatidylethanolamine was damaged by peptide and released 45Ca dose dependently.
홍화자 추출물과 키토산 병용처리에 의한 경조직 재생촉진 효과
정세영(Se Young Choung),박준봉(Joon Bong Park),박건구(Kun Koo Park),권영혁(Young Hyuk Kwon),김성진(Sung Jin Kim) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.4
N/A This study was performed to investigate therapeutic effects of Carthami Semen, Paeoniae Radix extracts and chitosan on the growth and differentiation of human periodontal ligament cell. We found that co-treatment of methanol extracts of Carthami Semen and chitosan significantly increased the growth of human periodontal ligament cell. However, the sigle treatment groups of the extracts showed only 20∼30% of the growth increase. Alkaline phosphase activity, one of differentiation markers, was increased approximately 1.5-fold by co-treatment of methanol extract of Carthami Semen and chitosan and calcified nodule formation was also increased at the similar levels as the alkaline phosphatase. But the single treatment groups showed only 20∼30% increases. These results suggest that Carthami Semen and chitosan co-treatment can be used efficiently for periodontium regeneration.
혈장내 Pentoxifylline과 Metabolite(I)의 HPLC에 의한 분석
민병선,정세영,노영수,윤병호,임수한,Min, Byeong-Sun,Choung, Se-Young,Rho, Young-Soo,Youn, Byoung-Ho,Lim, Soo-Han 대한약학회 1988 약학회지 Vol.32 No.6
A rapid and simple method for the determination of pentoxifylline and its major metabolite, metabolte(I) in plasma was examined by HPLC. For the purification and enrichment of drug from plasma, solid-phase extraction was examined using Sep-pak C18 cartridge. The effectiveness of test method was proved by monitoring of the rat after oral administration of pentoxifylline in a dose of 40mg/kg.