제주도내 하천의 부착규조 군집

정명화 ( Myoung Hwa Jung ),조현진 ( Hyeon Jin Cho ),윤정하 ( Jung Ha Yun ),이학영 ( Hak Young Lee ) 한국환경생물학회 2014 환경생물 : 환경생물학회지 Vol.32 No.1

본 연구는 제주도내 위치하는 상시 유수가 흐르는 6개의 하천을 선정하여 이화학적 요인과 부착 규조류 군집의 분포를, 2008년에서 2012년까지 연 2회 조사하였다. BOD는 0.0~1.6 mg L-1의 범위로 높은 수질 상태를 나타냈으며, TN, NH3-N, NO3-N, TP와 PO4-P는 각각 평균 4.432 mg L-1, 0.069 mg L-1, 3.822 mg L-1, 0.092 mg L-1, 0.071 mg L-1으로 비교적 높은 영양염의 농도를 나타냈다. 조사기간 동안 출현한 부착 규조류는 총 171종으로 2목 4아목 10과 28속 150종 17변종 2품종 2아종으로 구성되어 있었으며, 주요 우점종은 광적응성종인 Achnan-thes lanceolata, Melosira varians, 호청수성종인 Gomphonema pumilum, 호오탁성종인 Navicula minima, Nitzschia palea 등으로 나타났다. DAIpo와 TDI를 이용한 생물학적 수질 평가에서는 각각 평균 56.0 (보통, C등급), 70.4 (불량, D등급)으로 제주도내 하천의 수생태건강성은 낮은 것으로 나타났다. To identify distribution of epilithic diatom assemblages and the relationship between diatoms and water chemistry, samples were collected twice a year from 2008 to 2012, from at 6 streams in Jeju Island, South Korea. A total of 171 diatom taxa were identified, and Achnanthes lanceolata, Gomphonema pumilum, Melosira varians, Navicula minima and Nitzschia palea were dominantly observed. BOD ranged 0.0~~1.6 mg L--1, TN 0.230~~13.240 mg L--1 (average 4.432 mg L--1), TP 0.020~~0.316 mg L--1 (average 0.092 mg L--1), and N/P rate ranged 2.5~~178.5. Average of epilithic algal chlorophyll-a and AFDM were 1.1 μμg cm--2 and 0.6 mg cm--2, respectively. According to BOD standard, the water qualities of study area sites were at ‘Good’ level. However, the healthiness levels assessed from DAIpo and TDI were ‘Fair’ to ‘Poor’.

영산강,섬진강 수계 호소의 규모에 따른 식물플랑크톤 분포

나정은 ( Jeong Eun Na ),정명화 ( Myoung Hwa Jung ),박종환 ( Jong Hwan Park ),김상돈 ( Sang Don Kim ),임병진 ( Byung Jin Lim ),김현우 ( Hyun Woo Kim ),이학영 ( Hak Young Lee ) 한국환경생물학회 2011 환경생물 : 환경생물학회지 Vol.29 No.2

The relationships between the phytoplankton community and sizes of reservoirs are investigated from 29 reservoirs in Yeongsan and Seomjin River Basins, Korea. As a microalgal flora, a total of 371 species of phytoplankton were identified. There were spatial and seasonal variations in standing crops and species diversity of phytoplankton. Statistical analysis showed that the size of reservoirs did not affect greatly on the community of phytoplankton. Species diversity and standing crops were higher in reservoirs of smaller surface area. However, there were no distinctive relationships between the size of basins of reservoirs and standing crops, species diversity, and chlorophyll a concentrations. Relationships between the constructed years of reservoirs and standing crops, species diversity, and chlorophyll a concentrations also showed very low level.

미국-멕시코-캐나다 협정(USMCA) 수산조항에 관한 연구

안지은(Ji Eun An),정명화(Myoung-Hwa Jung) 한국해양비즈니스학회 2020 해양비즈니스 Vol.- No.45

On March 13, 2020, the domestic approval process for Canada, the last ratified member of the United States-Mexico-Canada Agreement (USMCA), was completed. Accordingly, the USMCA, signed on October 1, 2018, will be officially into force within the next three months. This means that the North American Free Trade Agreement (NAFTA) system, which has been in charge of trade policy between the three notrh american countries in the past 25 years, has ended, and a new trade environment called the USMCA has been created. The USMCA is the result of the renegotiation of the NAFTA, a key commitment highlighted by the US Trump government, and is a representative product of the US government s policy to strengthen protectionism. Due to the strong commitment of the US to renegotiate NAFTA, the the United States have took the lead of the USMCA in the form of bilateral agreements with Mexico and Canada respectively. In addition, the structure and contents of the agreement have similar characteristics to the TPP, which the US led in the past. The USMCA Fisheries Provisions are contained in Chapter 24(Environment) of the Agreement and consist of a total of five provisions: the importance of sea catching fisheries, fisheries management, marine life conservation, fisheries subsidies regulation and notification, and IUU fishing regulations. The USMCA Fisheries Provisions have the following important meanings in the formation of international fisheries norms. First, it is the only multilateral trade agreement signed by the United States, including specific and comprehensive fisheries regulations. Second, it is likely to be the basis for establishing fisheries regulations under bilateral and multilateral agreements, such as the future FTA and WTO fishery subsidies negotiation.

영산강,섬진강 수계 주요 호소의 식물플랑크톤 군집

나정은 ( Jeong Eun Na ),정명화 ( Myoung Hwa Jung ),조인숙 ( In Sook Cho ),박종환 ( Jong Hwan Park ),황경섭 ( Kyung Sub Hwang ),송효정 ( Hyo Jeong Song ),임병진 ( Byung Jin Lim ),라긍환 ( Geung Hwan La ),김현우 ( Hyun Woo Kim ) 한국환경생물학회 2012 환경생물 : 환경생물학회지 Vol.30 No.1

Abstract - The distributions of phytoplankton community are investigated from 29 reservoirs in Yeongsan and Seomjin River Basins, Korea. As a microalgal flora, a total of 424 species of phytoplankton were identified. They were 157 Chlorophyceae, 161 Bacillariophyceae, 39 Cyanophyceae, and 67 other algal taxa. The phytoplankton diversity were low in stream type reservoirs such as Kumho, Youngsan and Youngam Reservoirs. The population density of phytoplankton ranged from 29 cells mL-1 to 53,161 cells mL-1. The dominant phytoplankton species more than 20% of total standing crops were Asterionella formosa, Aulacoseira ambigua, Aulacoseira distans, Aulacoseira granulata, Cyclotella sp., Synedra acus, Coelastrum reticulatum, Pandorina morum, Scenedesmus arcuatus, Aphanothece clathrata, Microcystis aeruginosa, Phormidium sp. The concentration of chlorophyll a ranged from 0.41 mg m-3~220.72 mg m-3, and showed highest concentration in Gaecho-je. The concentrations of TN and TP have no clear relationships with chlorophyll a concentrations.

광주천의 식물플랑크톤과 부착조류의 동태

조혜경 ( Hye Kyung Cho ),정명화 ( Myoung Hwa Jung ),김규만 ( Gyu Man Kim ),심성순 ( Sung Sun Shim ),이학영 ( Hak Young Lee ) 한국환경생물학회 2010 환경생물 : 환경생물학회지 Vol.28 No.2

The distributions of planktonic and periphytic algae were studied from April 2003 to February 2004 at Gwangju Stream. A total of 274 species, belongs to 8 classes, 19 orders, 6 suborders, 35 families, 4 subfamilies, 79 genera, was identified as algal flora of Gwangju Stream. The most diverse phytoplankton flora was observed at Bangrim bridge and Yangdong market sites with 137 species, and followed by Yuchon bridge site (118 species). The richness of phytoplankton fluctuated throughout the studied period from all the sampled sites. The precipitation was a major factor affected the richness of phytoplankton. The diatoms predominated the phytoplankton of Gwangju Stream with 57.7~77.0% of total phytoplankton. The maximum cells of phytoplankton was observed at Yuchon bridge site with 23,792 cells mL-1 in August 2003, and minimum at Jeungsimsa site with 82 cells mL-1 in April 2003. The periphyton of Gwangju Stream was predominated by the diatoms. In February 2004, all the periphyton of Yuchon bridge site were diatoms. Other sites also showed the predominance of diatoms throughout the studied period. The standing crops of phytoplankton were highly correlated with chlorophyll-α concentration with R=0.795. TN and TP also showed high relationships with phytoplankton standing crops in Yangdong market site with R=0.796 and R=0.760, respectively. The rains of 7-days ago showed high relationships with phytoplankton standing crop at Dongsan tower site with R=0.810. However, other climatic factors showed no clear relationships with phytoplankton cells.

함평만 갯벌의 저서규조류 분포 특성

이학영 ( Hak Young Lee ),정명화 ( Myoung Hwa Jung ) 한국환경생물학회 2011 환경생물 : 환경생물학회지 Vol.29 No.1

The distributional pattern of benthic diatoms in tidal flats of Hampyeong Bay, Korea, was studied from January to October in 2009. As benthic diatoms of Hampyeong Bay tidal flats, 45 species were identified, and the most dominant species was Paralia sulcata. The most diverse flora was observed at Gaip and Songseok sites in April with 22 species, and the least at Hyeonhwa site in January. The ranges of chlorophyll-a concentration in tidal flats were 21.2~31.8 mg m-2 at Hyeonhwa site, 23.6~35.4 mg m-2 at Gaip site, and 24.2~34.3 mg m-2 at Songseok site. The concentrations of pheopigment ranged between 25.3 and 45.2 mg m-2. The standing crops of benthic diatoms showed highest density in April and lowest in January, February, and October. The cell volumes of benthic diatoms were highest in April. The taxa and biomass of benthic diatoms showed correlations with temperature. On temperature variables, the benthic diatoms showed optimal occurrences at the range of 14~17℃.

벤지미다졸계 살균제 베노밀과 카벤다짐을 분해하는 Rhodococcus sp. 균주들의 분리 및 특성 분석

이재철(Jae-Cheol Lee),전유나(Yu-Na Jeon),정명화(Myoung-Hwa Jung),김유진(Yu-Jin Kim),박인철(InCheol Park),유재홍(Jaehong You),이창묵(Chang-Muk Lee),한병학(Byeong-Hak Han),안시현(Si-Hyun An),안재형(Jae-Hyung Ahn) 한국농약과학회 2020 농약과학회지 Vol.24 No.2

대동맥판막 치환술 환자에서 발생한 아스페르길루스 심내막염 1예

신재령 ( Jae Ryung Shin ),김승민 ( Seung Min Kim ),유정선 ( Jung Sun Yoo ),오치혁 ( Chi Hyuk Oh ),정명화 ( Myoung Hwa Jung ),박지윤 ( Ji Yoon Park ),문수연 ( Soo Youn Moon ),손준성 ( Jun Seoung Son ),이미숙 ( Soo Cheol Kim ),김수 전북대학교 의과학연구소 2013 全北醫大論文集 Vol.37 No.1

아스페르길루스 심내막염은 국내에서도 7증례만 보고된 바 있는 드문 질환이며 명확한 치료 방안이 정립되지 못하여 사망률 또한 매우 높은 질환이다. 최근 심장수술의 증가, 장기이식, 광범위 항생제 사용 등으로 입원환자 중에 그 발생빈도가 증가하고 있어 해당 질환에 대한 관심이 필요하겠다. 현재까지 아스페르길루스 심내막염의 치료는 외과적 수술 및 적절한 항균제의 사용이 고려되고 있으나 병합요법의 필요성 및 추후 이차적 예방에 관해 더 많은 연구가 요구되고 있다. 저자들은 대동맥판막 치환술및 관상동맥 우회술을 시행한 환자에서 판막파열 및 가성동맥류가 발생하였고, 수술 후 조직검사에서 A. fumigatus를 확인했던 아스페르길루스 심내막염 환자를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Aspergillus endocarditis is a rare infection that occurs most commonly after heart valve replacement surgery. The outcomes for patients with prosthetic valve endocarditis due to Aspergillus species have been extremely poor. Therapeutic management conventionally consists of combined medical and surgical approaches. However, there was no clear evidence to determine whether or not combination therapy was superior to liposomal amphotericin B alone. And no clear pattern could be determined in relation to the optimal duration of initial antifungal treatment or of long-term secondary prophylaxis. We report a case of aspergillus endocarditis in a patient who underwent aortic valve replacement surgery. The results of repeated blood culture were negative. Aortic root replacement was performed and Aspergillus fumigatus was isolated from aortic valve tissue. Despite of postoperative liposomal amphotericin B therapy, he died of multiple organ failure associated with diffuse brain damage.

COI 기반 제한효소 절편 길이 다형성(RFLP)을 이용한 새우젓 분석

박주현(Park Ju Hyeon),문수영(Moon Soo Young),강지혜(Kang Ji Hye),정명화(Jung Myoung Hwa),김상조(Kim Sang Jo),최희정(Choi Hee Jung) 한국생명과학회 2021 생명과학회지 Vol.31 No.1

국내 유통되고 있는 새우젓은 다양한 작은 새우의 집합체로, 최근 어획량 감소로 인해 국내산 새우젓의 경우 수입산에 비해 두배 이상 높은 가격에 거래되고 있으며, 이에 중국 및 베트남 등으로부터 수입된 새우젓이 국내산으로 둔갑되어 판매되는 사례가 빈번하게 발생되고 있다. 본 연구에서는 새우젓 Acetes japonicus, A. chinensis(Korea, China), A. indicus (Ⅰ, Ⅱ), Palaemon gravieri 6종류에 대하여 PCR-RFLP 마커를 개발하였다. 새우젓에서 에탄올로 염을 제거한 후 gDNA를 추출하였고, 새우젓 COI 유전자의 특이 프라이머를 제작, PCR을 진행하여 519 bp의 증폭시켰다. 증폭된 PCR산물의 염기서열분석을 토대로 Acc I, Hinf I 두 가지의 제한효소를 마커로 선정하였고, 전기영동을 통해 결과를 확인하였다. Acc I을 처리한 결과, A. japonicus, A. chinensis (Korea), A. indicus (Ⅱ)는 272, 247 bp로, P. gravieri는 271, 202, 46 bp, A. chinensis (China), A. indicus (Ⅰ)는 519 bp로 band를 형성하는 것을 확인할 수 있었다. 또한 Hinf I을 처리한 결과로는 A. chinensis (Korea, China), P. gravieri는 519 bp로 잘리지 않은 것을 확인한 반면, A. japonicus와 A. indicus (Ⅰ)는 2 band, A. indicus (Ⅱ)는 3 band를 형성하는 것을 확인할 수 있었다. 따라서 위의 결과는 국내산과 수입산이 혼합되어있는 새우젓에 있어 보다 신속한 종판별을 할 수 있음을 시사한다. This study developed a species identification method for the salted opossum shrimp of Acetes japonicus, A. chinensis (Korea, China), A. indicus (Ⅰ, Ⅱ), and Palaemon gravieri based on PCR-RFLP markers. Genomic DNA was extracted from the salted opossum shrimp. The COI gene was used to amplify 519 base pairs (bp) using specific primers. The amplified products were digested by Acc I and Hinf Ⅰ, and the DNA fragments were separated by automated electrophoresis for RFLP analysis. When the amplified DNA product (519 bp) was digested with Acc I, A. japonicus, A. chinensis (Korea), and A. indius (Ⅱ) showed two fragments, whereas a single band of 519 bp was detected in A. chinensis (China) and A. indius (Ⅰ). Also, in the RFLP patterns digested by Hinf Ⅰ, A. chinensis (Korea) and A. chinensis (China) showed a single band of 519 bp, while two fragments were observed in A. japonicus and A. indius (Ⅰ) and four fragments in A. indius (Ⅱ). The PCR amplicon of P. gravieri was digested by Acc I into 3 bands of 271, 202, and 46 bp and by Hinf I into a single band of 519 bp. Therefore, salted opossum shrimp-specific RFLP markers showing distinct differences between four species and two sub-species by PCR-RFLP analysis. Thus, the PCR-RFLP markers developed in this study are a good method for identifying the six types of salted opossum shrimp.

증례 : 식도암 환자에서 혈관 내 스텐트 삽입으로 치료한 대동맥 식도 누공 1예

윤경한 ( Kyung Han Yoon ),장재영 ( Jae Young Jang ),오신주 ( Shin Ju Oh ),박유민 ( Yoo Min Park ),정명화 ( Myoung Hwa Jung ),김정욱 ( Jung Wook Kim ),장영운 ( Young Woon Chang ) 대한내과학회 2015 대한내과학회지 Vol.88 No.2

Hemorrhage into the esophagus due to aortoesophageal communication is very rare but life-threatening with high morbidity and mortality. Because of this, most cases of aortoesophageal fistula are confirmed by autopsy. However, we report herein a case of a 62-year-old male with an aortoesophageal fistula who was successfully treated with endovascular stent-grafting. The patient had undergone esophageal stent insertion due to esophageal cancer and experienced hematemesis and hemodynamic shock due to an aortoesophageal fistula. Emergency endoscopy was unable to identify the source of the hemorrhage due to massive bleeding. Computed tomography of the chest revealed a focal pseudoaneurysm at the descending aorta and diffuse thinning of the esophageal wall, which were treated with endovascular stent-grafting. Although aortoesophageal fistulas are usually fatal, implantation of a covered stent into the esophagus may help prevent massive bleeding, providing valuable time to treat the patient. (Korean J Med 2015;88:182-186)