아카시아 꽃 추출물의 피부미용 화장품 소재 생리활성 연구

장한솔 ( Han-sol Jang ),장아람 ( Ah-ram Jang ),송연숙 ( Yeon-sook Song ),김명곤 ( Myung-kon Kim ),이근광 ( Keun-kwang Lee ) 한국미용학회 2015 한국미용학회지 Vol.21 No.5

This study analyzed the composition and contents of acacia(Robinia pseudoacacia) flower extracts, free amino acid of water and methanol extracts and polyphenol compound. Then, the physiological activities of some skin care cosmetic examined. The study results found the followings: The free amino acid of acacia flower hot water extracts was 36.96 mg/g in total, and a total 16 types of free amino acids were isolated. In methanol extract, however, a total of 13.41 mg/g were found in 13 different types. In terms of polyphenol contents, methanol extract was slightly higher than hot-water extract. Regarding the composition of polyphenol compound, 8 different types of phenolic acids and two types of flavonoids were found in hot-water and methanol extracts. The DPPH radical scavenging activity was increased in proportion to the concentration, and the inhibitory activity of tyrosinase in the hot-water and methanol extracts of acacia flower was increased in proportion to the concentration. The ell growth according to human skin cell line, HaCaT was very low cell growth inhibited in an concentration-dependent. The inhibitory activity of elastase and collagenase in the hot-water extract and methanol extracts increased in a concentration-dependent manner. The said results show that because free amino acid and polyphenol contents are rich in the hot-water extract of acacia and methanol extract, they could be very useful as cell growth activating factors or natural antioxidants. To use them as cosmetic ingredients, however, there should be further studies on correlations between the enzyme activity in the cell and various cosmetic components.

참마의 괴경과 영여자 추출물의 Phenolic Acids와 Flavonoids의 조성 및 피부미용 화장품 생리활성 검색

장아람 ( Ah Ram Jang ),이근광 ( Keun Kwang Lee ),김명곤 ( Myung Kon Kim ) 한국미용학회 2016 한국미용학회지 Vol.22 No.1

The purpose of this study was to investigate the phenolic compound compositions and skin care cosmetic physiological activities of Dioscorea japonica freeze drying yam‘s tuber, freeze drying and hot-air drying aerial bulbil extracts with MeOH solution. Total polyphenol content was higher in the yam tuber extract than aerial bulbil extract. Total flavonoid content of freeze-drying and hot airdrying aerial bulbil extract indicating higher than about 6.3 and 5.2 times content in the yam tuber extract, respectively. The major phenolic compounds of the yam tuber, freeze-drying and hot air-drying aerial bulbil extracts was p-hydroxybenzoic acid, sinapic acid and t-cinnamic acid respectively. Similarly, the flavonoids of the yam tuber, freeze-drying and hot air-drying aerial bulbil extracts were myricetin(6.02~7.43 mg%) and isorhamnetin(3.07~5.41 mg%), respectively. DPPH radical scavenging activity of freeze-drying and hot air-drying aerial bulbil extract was higher than yam tuber extracts. Tyrosinase inhibition activity of yam tuber extract indicating higher than about 32% and 22.35% freeze-drying and hot air-drying aerial bulbil extracts, respectively. The antimicrobial activities of freeze-drying yam tuber extract and hot-air drying aerial bulbil extracts was found that; E. coli KCCM 11234 and S. aureus KCCM 11593 cell growth was some inhibited at 1000 μg/ml concentration. Whereas, S. aureus KCCM 11593 cell growth was some inhibited by freeze-drying and hot-air drying aerial bulbil extracts. In addition, cytotoxicity for NIH3T3 cell line was not appeared by yam tuber, freeze drying and hot-air drying aerial bulbil extracts. These results indicated that D. japonica yam tuber and aerial bulbil extracts are functional and effective. In particular, they would be very useful as a health food and whitening cosmetic ingredients.

무궁화나무(Hibiscus syriacus) 수피 추출물의 페놀산 조성 및 기능성화장품 소재 가능성 평가

장아람 ( Ah-ram Jang ),송연숙 ( Yeon-sook Song ),김명곤 ( Myung-kon Kim ),이근광 ( Keun-kwang Lee ) 한국미용학회 2015 한국미용학회지 Vol.21 No.5

The purpose of this study was to investigate the phenolic acid contents and compositions, and possibility of functional cosmetic ingredients of Hibiscus syriacus bark extracts with hot water and 70% ethanol solution. The results were as follows: Total polyphenol and total flavonoid content, hot water extract was grater than 70% ethanol extract. Regarding the composition of polyphenol compound, 8 different types of phenolic acids and 4 different types of flavonoids were found in hot-water and 70% ethanol extracts. In terms of DPPH radical scavenging activity, water extract was about 2 times grater than 70% ethanol extract. Nitrite-scavenging activity against hot water extract was 52.30 ± 0.42% and for the 70% ethanol extract was 46.00 ± 0.52% of stem bark in pH 1.2, respectively. In the tyrosinase inhibition activity of IC50, hot water extract was about 1.6 times grater than 70% ethanol extract. Bacterium cell growth was inhibited the proportional to the concentration for both the hot water and 70% ethanol extracts of bark for the Gram-negative E. coli KCCM 11234 bacterium. Cytotoxicity for SK-MEL-2 cell line was not appeared for hot water, and appeared about 16% in the 100 μg for 70% ethanol extract.

피부미용 화장품 소재로서 연복자 추출물의 유리아미노산 및 유리당의 조성과 함량분석

장아람 ( Ah-ram Jang ) 한국미용학회 2021 한국미용학회지 Vol.27 No.2

This study analyzed pericarp of Akebia quinata fruit and hot water extract of seeds and 80% methanol extract to verify the effectiveness of utilizing functional cosmetic materials for skin care. 17 components of free amino acids were identified in pericarp and seeds. Both pericarp and seeds had the highest lysine content in hot water extracts and asparagine in 80% methanol extracts. Protein-free amino acid derivatives were identified in pericarp and seeds, each with 5 components. In the hot water extract, phosphoserine was the highest in pericarp and citrulline was the highest in seeds. In 80% methanol extract, carnosine was the most common in pericarp and citrulline was the highest in seeds. Analysis of the composition of free sugar identified frutose and glucose in pericarp and sucrose and maltose in seeds. As a result, when pericarp and seeds of Akebia quinata fruit are extracted with hot water and 80% methanol, free sugar and free amino acid derivatives are present, which is expected to be valuable as functional foods and nutritional cosmetics ingredients for skin beauty.

신장이식환자를 위한 약물교육프로그램이 약물에 대한 지식과 약물복용 이행도에 미치는 효과

장정주(Jang, Jeong Ju),전정애(Jeun, Jeong Ah),박정숙(Park, Jeong Sook),성은혜(Sung, Eun Hae),김혜림(Kim, Hye Rim),조아람(Jo, A Ram) 계명대학교 간호과학연구소 2017 계명간호과학 Vol.21 No.1

Purpose: The purpose of this study was to analyze the effects of the drug educational program on knowledge and medication compliance for kidney transplantation patients. Methods: Kidney transplantation patients were recruited from a transplantation center, at a university hospital located in D city, Korea. Data were collected from June 18m 2016 to september 30, 2016. The research design was a nonequivalent one group pre-posttest. Nineteen subjects participated twice in a drug educational program given by the researcher. Results: After the intervention, the participants showed a significant increase in medication knowledge (t=-7.074, p<.001) and medication compliance (t=-4.553, p<.001) than before the intervention. Conclusion: The research findings suggest that a drug education program can be used as an effective nursing intervention for kidney transplantation patients.

연복자 추출물의 Phenolic acids와 Flavonoids의 조성 및 화장품 항산화 활성

장아람,Jang, Ah-Ram 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.4

이 연구는 연복자의 열수와 80% 메탄올 추출물에 대한 총 polyphenol과 flavonoid의 함량과phenolic acid와 flavonoid의 조성 및 함량을 분석하고, 이 추출물에 대한 일부 항산화 활성을 조사하므로 스킨케어 기능성 화장품으로의 활용 가능성을 확인하는 것이 연구의 목적이다. 총 polyphenol과 flavonoid 함량은 열수와 80% 메탄올 추출물 모두에서 과피 추출물이 씨앗 추출물 보다 높은 것으로 나타났다. Phenolic acid는 열수 추출물에서 2종, 80% 메탄올 추출물에서 6종이 확인되었다. Flavonoids는 열수 추출물에서는 확인되지 않았으나, 80% 메탄올 추출물에서는 1종이 확인되었다. 또한 연복자의 열수와 80% 메탄올 추출물에 대한 항산화 활성을 조사한 결과, DPPH와 ABTS의 라디칼 소거능은 처리농도에 비례하여 증가하는 경향을 나타내어 항산화 활성이 높은 것으로 나타났다. 따라서 이와 같은 결과를 종합하여 보았을 때 연복자의 열수와 80% 메탄올 추출물은 항산화 활성을 나타내는 물질을 함유하고 있고, 항산화 활성이 확인되어 피부관리를 위한 기능성 화장품 성분원료 및 천연 항산화제로서의 가능성이 있을 것으로 사료되어진다. In order to verify the effect of using functional cosmetic ingredients for skin beauty, the composition and content of some phenolic acid and flavonoids in the hot water and 80% methanol extract of Akebia quinata fruit pericarp and seeds were analyzed, and the skin care antioxidant activity was investigated. The results are as follows. Total polyphenol and flavonoid contents were found to be higher in pericarp extract than seed extract in both hot water and 80% methanol extract. In the analysis of the composition and content of phenolic acid and flavonoids, two kinds of phenolic acids were identified in the hot water extract of pericarp, 6 kinds of phenolic acids were identified in the 80% methanol extract, and one kind of flavonoid. Two types of phenolic acids were identified in the hot water and 80% methanol extract of seeds, respectively. DPPH and ABTS radical scavenging activities tended to increase in proportion to the treatment concentration in both hot water and 80% methanol extracts, and antioxidant activity was found to be high. Therefore, from the above results, it was found that the hot water and 80% methanol extract of Akebia quinata fruit pericarp and seeds contained various kinds of phenolic acid and flavonoids, and the antioxidant activity was also high. It is believed to be of value as a natural antioxidant in skin care cosmetic ingredients.

급성 신부전 환자에서 지속적 신대체요법을 이용한 마취관리 -증례 보고-

두아람 ( A Ram Doo ),장은주 ( Eun Joo Jang ),김덕규 ( Deok Kyu Kim ) 전북대학교 의과학연구소 2014 全北醫大論文集 Vol.38 No.2

The treatments for acute renal failure include recovery of renal perfusion, correction of reversible cause, normalization of plasma electrolytes and renal replacement therapy. Continuous renal replacement therapy (CRRT) is preferred to intermittent hemodialysis due to its superior hemodynamic stability with resultant effective elimination of water and toxins in critical care fields. We report a case of successful anesthetic management using CRRT in patient receiving emergent small bowel resection with acute renal failure complicated by sepsis.

Association Study between Genetic Polymorphisms of CYP2C19 Gene and Essential Hypertension in Koreans

Ah-Ram Park(박아람),Eun-Soon Shin(신은순),Nak-Hoon Son(손낙훈),Yangsoo Jang(장양수),Dong-Jik Shin(신동직) 한국생명과학회 2010 생명과학회지 Vol.20 No.5

혈관 수축력 및 혈압 조절에 관여하는 것으로 알려진 아라키돈산을 물질 대사시키는 CYP2C19 유전자는 최근 심혈관 질환 관련 연구의 새로운 유전자로 제시되고 있다. 본 연구에서는 CYP2C19 유전자의 2 종류 다형성(CYP2C19<SUP>*</SUP>2와 CYP2C19<SUP>*</SUP>3)과 고혈압 간의 연관성을 조사하고자 하였다. 연세대학교 의료원 심장혈관병원에서 수집한 1,241명(환자군: 537명, 대조군: 704명)을 대상으로 SNaPShot™ assay를 이용하여 유전자형을 결정하였다. 두 종류의 다형성 가운데 CYP2C19<SUP>*</SUP>3의 대립인자형 및 유전자형의 빈도 분포가 환자군과 대조군 간에 유의한 차이를 나타냈다(p=0.019, p=0.023). 다중 로지스틱 회귀분석 결과, dominant model에서, CYP2C19<SUP>*</SUP>3 A 대립인자형은 본태성 고혈압과 매우 유의한 연관성을 나타냈다(OR, 0.723, p=0.032). 또한 CYP2C19 G-A haplotype은 고혈압 발생 위험을 매우 유의하게 감소시키는 것으로 조사되었다(OR, 0.714, p=0.015). 따라서 본 연구 결과는 CYP2C19<SUP>*</SUP>3 다형성이 본태성 고혈압 발생에 대한 보호 효과작용에 관여할 것이라는 증거를 제시하고자 한다. In humans, CYP2C19, a member of the cytochrome P450 subfamily, metabolizes arachidonic acid to produce epoxyicosanoid acids, which are involved in vascular tone and regulation of blood pressure (BP). Recent findings suggest that CYP2C19 gene polymorphisms might be considered as a novel candidate gene for cardiovascular disease. We thus focused on the Korean population to explore the association of two polymorphisms (CYP2C19<SUP>*</SUP>2 and <SUP>*</SUP>3) in this gene and essential hypertension (EH). A total of 1,241 participants (537 hypertensive subjects and 704 healthy controls) were recruited from the Yonsei Cardiovascular Genome Center in Korea. The CYP2C19 polymorphisms were genotyped using the SNaPShot™ assay. The allele and genotype frequencies of CYP2C19<SUP>*</SUP>3 showed significant difference between hypertensives and normotensives (P=0.019 and P=0.023, respectively). Logistic regression analysis indicated that the CYP2C19<SUP>*</SUP>3 A allele carriers were significantly associated with EH (OR, 0.723; 95% CI, 0.538-0.972, P=0.032) under a dominant model. In addition, CYP2C19 G-A haplotype (2C19<SUP>*</SUP>2 G-<SUP>*</SUP>3 A combination) was found to significantly reduce EH risk (OR, 0.714, P=0.015). We believe this provides evidence that CYP2C19<SUP>*</SUP>3 polymorphism may contribute to a protective effect in the development of EH.

작업치료과 학생들이 지각한 작업치료사의 이미지의 대한 연구

송민옥 ( Min Ok Song ),장아람 ( A Ram Jang ),정명진 ( Myeong Jin Jung ),홍미애 ( Mi Ae Hong ),장철 ( Chel Jang ) 대한통합의학회 2013 대한통합의학회지 Vol.1 No.3

Purpose : The purpose of this study work for the therapy and how students perceive the occupational therapist was to find out how. Method : The subjects in Busan and Kyungnam located in three two-year colleges and four-year clinical experience of two college students completed work therapeutics were expressed any research to understand the purpose of those who agreed to participate in the survey responded to the 175 people working Therapeutics two, three, four grades were included in the study. Result : 1. Education according to the image of the occupational therapist in grades four -year professional, the value and worth, the patient understand the position was higher in order. 3 -year sophomore highly skilled in, the patient understand the position, sincerity was higher in order. 2. Gender occupational therapist who specializes in images for men, the value and worth, high practician was higher in women who were professional, the value and worth, helfer as high. 3. Theory of motivation based on the value of occupational therapists and rewarding images aptitude and interest, high practician, attractive, intellectual was in order. Entrance examination scores as a professional party, value & worth, abuse of authority ceremony was in order. 4. Image by Place of Practice Rehabilitation Hospital occupational therapist who specializes in, high practician, intellectual was in order. Care in the hospital and rewarding value, the professional character, high practician was in order. Hospital and rewarding the value, who specializes in, hepfer was in order. University Hospital who specializes in, the value and worth, Attractive was in order. Conclusion : It had revealed that occupational image perception of occupational therapy student was positive.

미용 서비스산업 종사자들의 두피,모발 관리 교육 및 만족 정도

서미영 ( Mi Young Seo ),장아람 ( Ah Ram Jang ),송연숙 ( Yeon Sook Song ) 대한미용문화예술학회 2014 대한미용문화예술학회지 Vol.3 No.1

This study was education and degree of satisfaction of scalp and hair care of beauty service industry workers. The results are as follows: Regarding the education on scalp and hair care, in general, they received scalp care educations through the education of the products company mainly less than 10 times. The higher their academic background, job title, or salary, the more they have experience and times in receiving education. The received educations for activation of scalp and hair care were the scalp massage(23.5%), physiological function(23.1%) and counseling technique(22.4%) for scalp and hair care related to scalp and hair, diagnosis and judgement of scalp, education for clinic products, and machine usage. With above results, It is urgently required to develop various programs for activation of the treatment for scalp and hair care as well as improve the customer`s awareness by raising customer`s satisfaction through more systematic education.