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피혁 폐기물로부터 collagen 추출과 크롬이온 분리에 관한 연구
임봉주(Bong-Ju Lim),임남웅(Nam-Uoong Lim),임한진(Han-Jin Lim) 유기성자원학회 1998 유기물자원화 Vol.6 No.1
본 연구는 피혁 폐기물로부터 크롬 이온을 분리한 상태로 Collagen을 추출할 수 있는 최적 조건에 관한 것이다. Col1agen 추출에 있어 온도, pH, 그리고 알칼리 용액 농도의 영향을 설험했다. 실험결과 점도측정에 의해 초기 변성온도는 250C, 완전 변성온도는 31. 50 C 였다. Collagen 추출의 최적조건은 가용화 온도 150C-200C, pH 1. 5, 알칼리 용액 3%였다. pH 1. 5에서 크룸 이온이 거의 분리되었다. 탄년 폐기물로부터 크롬 이온의 분리효율은 99.5% 이상이였다. 피혁 폐기물로부터 조단백질의 추출율은 89.% 이었다. 추출된 조단백질내에 hydroxyprolne과 collagen의 함량은 각각 8.53%, 63.62%였다. The objective of this study is to investigate the optimum conditions of extracting collagen without chrome ion from the leather waste. The effect of temperature, pH, and the concentration of alkaline solution on the collagen extraction has been studied. The result indicated that the incipient denatured temperature of collagen measured by viscosity was 250C and the complete denatured temperature was 31. 50C. The optimum solubilization condition for temperature was between 150C and 20oC, pH was 1.5, the concentration of alkaline solution was 3% of sodium hydroxide. The almost complete chrome ion separation was possible around the pH of 1. 5. The separation efficiency of chrome ion from tannery waste was more than 99.5%. Extraction efficiency of crude protein from leather waste was about 89.5%. The hydroxyproline and col1agen content in the extracted crude protein were 8.53% and 63.62%, respectively.
안호근,손보균,임한진 순천대학교 공업기술연구소 1998 工業技術硏究所論文集 Vol.12 No.-
The reaction conditions were investigated to prepare the hardened oil with suitable properties from Camellia japonica oil by catalytic hydrogenation in batch reactor at atmosphere. The nickel-based catalysts were used, and the hydrogenation activity of the catalyst was measured as iodine value. The commercial Pricat-9920 catalyst was highly active. The hydrogenation rate over Pricat-9920 catalyst was increased with increasing reaction temperature to 180℃, but decreased over 180℃ because the dissolution of hydrogen in the oil was difficult at high temperature. Also, the rate depended on the amounts of catalyst used, the flow rate of hydrogen, and agitation rate, which were deeply related to the diffusion of hydrogen in the pore of the catalyst. The hardened oil with melting point 30~40℃ was obtained at reaction time 4~6hr, reaction temperature 180℃, the amounts of catalysts 0.2%Ni(w/v), and the flow rate of hydrogen 50cc min^(-1). The relationship between iodine value and melting point was linear, so the iodine value could be easily estimated from the melting point.