한국 남성 불임환자에서 Y 염색체상의 AZF Gene에 대한 분석 및 DAZ Gene의 발현 양상

이호준,이형송,송견지,변혜경,서주태,김종현,이유식,Lee, Ho-Joon,Lee, Hyoung-Song,Song, Gyun-Jee,Byun, Hye-Kyung,Seo, Ju-Tae,Kim, Jong-Hyun,Lee, You-Sik 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.1

Cytogenetic observations of loss of the distal portion of the Y chromosome long arm were found to be associated with disrupted spermatogenesis. The existence of a gene involved in the regulation of spermatogenesis, the azoospermia factor (AZF), was postulated. In this study, we screened the AZF region including DAZ and DAZH genes and observed the expression pattern of DAZ and DAZH transcript in infertile men with azoospermia and oligospermia by using a sequence-tagged site (STS)-based PCR method. PCR primers were synthesized for 11 STSs that span Yq interval 6, SRY, DAZ, and DAZH, functional DAZ homologue on chromosome 3. Microdeletions were detected in 4/32 (12.5%) azoospermic men and 1/11 (9%) severe oligospermic men. Only 2 of 5 patients had microdeletions of Yq that contained the DAZ gene, whereas the other 3 patients had deletions extending from intervals 5L-6F proximal to the DAZ gene on Yq. Testis biopsies of the azoospermic patients revealed a variety from Sertoli cell-only syndrome to testicular maturation arrest. Of 4 men with clinical data available, average testis size was R: 13.8 cc, L: 13.8 cc, serum T was $4.0{\pm}1.25$ ng/ml, LH was $3.63{\pm}1.90$ mIU/ml, and FSH was $8.85{\pm}5.13$ mIU/ml. These values did not differ significantly from the remainder of the patients tested. We could not observed the DAZ transcript in 2 patients, who have no mature spermatozoa. In 11.6% of patients microdeletions of the AZF could be detected. These deletions in the AZF region seem to be involved causing spermatogenic failure. But the frequency of microdeletions proximal to DAZ suggests that DAZ is not the only gene associated with spermatogenic failure.

Miscanthus sinensis 와 타 식물과의 친근성에 관한 연구

정일구(Il Koo Lee),이호준(Ho Joon Lee),이경순(Kyung Soon Lee) 한국식물학회 1970 Journal of Plant Biology Vol.13 No.4

Althought allelopathy has been cleary established for Pinus densiflora by Lee-Monsi, the auther suspected that Miscanthus sinensis which grows vigrously in such Korean pine forests might also exhibit the phenomenon. A total of 33 species having an affinity in the Miscanthus group were recorded in the field. Out of these, five species; Patrinia scabiosaefolia, Lespedeza crytobotrya. Oenothera odorata, Raphanus sativus var. acanthiformis, and Zoysia japonica were considered the A group. Five other species which have no affinity to Miscanthus sinensis, Amaranthus patulus, Solanum nigrum, Capsella bursa-pastorris var. triangularis, Chemopodium album var. centrorubrum and Alopeculus amurensis were considered the B group. Extracts of Miscanthus sinensis were applied to determine relative germination and growth. It was found the growth in the B group was promoted by a cold water extract of Miscanthus stems and leaves, but inhibited remarkably by an extract obtained by passing cold water through Miscanthus root. As expected, growth in the A group was promoted by both experimental treatments.

체외배양 생쥐정소세포에서 합성에스트로겐이 P450 등위효소의 발현에 미치는 영향

이호준,김묘경,고덕성,김길수,강희규,김동훈,Lee, Ho-Joon,Kim, Myo-Kyung,Ko, Duck-Sung,Kim, Kil-Soo,Kang, Hee-Kyoo,Kim, Dong-Hoon 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.2

Objective: To know the effects of xenoestrogen on spermatogenesis, we investigated the expression of cytochrome P450s enzymes (CYPscc, $CYP_{17{\alpha}}$, CYP19) and $3{\beta}$-HSD genes involved in steroidogenesis. Methods: Mouse testicular cells were prepared from 15-day-old ICR mice which had only pre-meiotic germ cells by enzyme digestion using collagenase and trypsin. Testicular cells were cultured in DMEM supplemented with FSH (0.1 IU/ml) and 10% FBS or medium with estrogen ($E_2$), bisphenol-A (BPA), octylphenol (OP; $10^{-9},\;10^{-7},\;10^{-6},\;10^{-5},\;10^{-4}M$, respectively) and aroclor 1254 (A1254) known as PCBs for 48 hours. The gene expression of cytochrome P450 enzymes were examined by semi-quantitive RT-PCR. The production of estrogen and testosterone was examined by RIA. Results: As results, expression of CYPscc mRNA was not significantly decreased, but $3{\beta}$-HSD and $CYP_{17{\alpha}}$. mRNA were significantly dose-dependent decreased. And production of testosterone and estrogen were not different except BPA and OP group ($10^{-5}M$). Conclusion: BPA, OP and A1254 might inhibit steroidogenesis by decreasing CYPscc, $3{\beta}$-HSD and $CYP_{17{\alpha}}$. mRNA expression in the mouse testis. These results suggest that BPA, OP and PCBs like as an endocrine disruptors inhibit the productions of steroidogenic enzymes and decrease the production of T and E by negative feedback mechanism. Therefore, these might disrupt steroidogenesis in Leydig cells of testis and would disturb testicular function and subsequently impair spermatogenesis.

프로피온산을 이용한 시리얼제품의 방충 포장

이호준,Lee, Ho-Joon 한국식품연구원 2011 食品技術 Vol.24 No.2

고해상도 레이더용 다중산란점 발생장치의 설계 및 제작

이호준,김윤진,윤승구,정해창,공덕규,이재웅,변영진,Lee, Ho-Joon,Kim, Youn-Jin,Yoon, Seung-Gu,Jeong, Hae-Chang,Kong, Deok-Kyu,Yi, Jae-Woong,Byun, Young-Jin 한국군사과학기술학회 2016 한국군사과학기술학회지 Vol.19 No.5

We designed the multiple scattering points generation system to simulate an actual situation of target signal for high range resolution radar system. This provides replicating the target signals and controlling the status of target signals for radar system. This is composed transmit antenna and multi target generator. Transmit antenna is waveguide array antenna and multi target generator has signal distribution module and control & power module. Multi target generator is able to provide the high isolation and variable output power. Moreover, in order to monitor all output signals of the multi target generator, the flows of signals are programed in control & power module. The performance is demonstrated using experimented results of high range resolution radar.

샘플링 광섬유 Bragg 격자를 이용한 광섬유 내의 유도 Brillouin 산란 억제

이호준,Lee, Ho-Joon 한국광학회 2005 한국광학회지 Vol.16 No.6

I have investigated a scheme for suppressing stimulated Brillouin scattering in optical fibers. The scheme makes use of a sampled Bragg grating fabricated within the fiber used for transmitting intense Q-switched pulses. The grating is designed such that the spectrum of the Stokes pulse generated through stimulated Brillouin scattering falls entirely within its stop band. I show numerically that the number of sampled fiber Bragg gratings in 1 m is applied directly to suppressing stimulated Brillouin scattering rather than the coupling coefficient. This prevents the build up of the backward-propagating Stokes wave and mitigates the deleterious effects of stimulated Brillouin scattering. The simulation shows that 15 ns pulses with 1 kW peak power can be transmitted though a 1 m-long fiber with little energy loss using this scheme. 본 논문에서는 광섬유 내에서 유도 브릴루앙 산란을 억제하는 방법에 대하여 연구하였다. 광섬유 내에 샘플링 브라그 격자를 사용함에 의하여 강한 Q-스위치 펄스를 전송할 수 있도록 하였다. 격자는 유도 블릴루앙 산란으로 생성되는 스토크 펄스의 스펙트럼이 모두 브라그 격자의 반사 대역에 오도록 설계된다. 1 m 내에 샘플링 광섬유 브라그 격자의 수가 결합계수 보다 유도 브릴루앙 산란의 억제에 직접적인 영향을 미친다는 것을 보였다. 이것은 후방 스토크 파의 발생을 억제하며 유도 브릴루앙 산란의 악 영향을 감소시킨다. 본 연구 방법을 통하여 1 kW 최대 파우어를 갖는 15 ns 펄스가 작은 에너지 손실을 발생시키며 전송될 수 있다는 것을 시뮬레이션을 통해 보였다.

월악산 신갈나무 ( Quercus mongolica ) 림의 종조성과 토양환경

이호준,전영문,김창호 ( Ho Joon Lee,Young Moon Chun,Chang Ho Kim ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2

The relationship between the floristic composition and soil environmental factors of the Quercus mongolica forest on Mt. Worak was investigated from May 1993 to February 1997. The Quercus mongolica -Lespedeza maximoiwiczii community was classified into two subcommunity of Quercus variabilis and Artemisia stolonifera. The dominance sequences on each stratum determined by the coverage index showed that Quercus mongolica was most dominant in tree-1 layer with 7569.6, and in tree-2 layer with 1211.4, Lespedeza maximoiwiczii in shrub layer with 896.9, and Ainsliaea acerifolia in Herb layer with 1480.8 value. The highest value of diameter breast height (DBH) was distributed in the range of 11 ∼ 20 ㎝ (41.1%) in Quercus mongolica forest and it showed a bell-shape distribution. Except soil water content, almost every data showed the lower value at 600∼850m than any other altitude. Species dominance was eminently Sasu borealis. Carex lanceolata at 400∼600m, Carex humilis at 600∼850 m and Fraxinus rhynchophylla, Acer pseitdo-sieboldianum, Ainsliaea acerifolia, Carex siderosticta at 860∼1060m, respectively. Soil conditions in NE-NW slope was more favorable than in SE-SW slope. In summary, Quercus mongolica, Acer pseudo-sieboldianum. Carex siderosticta, and Ainsliaea acerifolia were dominant in NE-NW slope, while Quercus mongolica, Fraxinus rhynchophylla, Spodiopogon sibiricus, and Carex humilis dominant in SE-SW slope.

광섬유의 음향센서 기법

이호준,박병철,Lee, Ho-Joon,Park, Byung-Chul 한국음향학회 1990 韓國音響學會誌 Vol.9 No.2

일본 식품 용기포장재의 안전성에 대한 규격과 최신동향

이호준,Lee, Ho-Joon 한국식품연구원 2009 食品技術 Vol.22 No.4

Mn 과 Zn 이 소리쟁이 ( Rumex crispus L. ) 의 발아와 유식물의 (幼植物) 생장에 미치는 영향

이호준,윤영진 ( Ho Joon Lee,Young Jin Yoon ) 한국환경생물학회 1994 환경생물 : 환경생물학회지 Vol.12 No.2

The amounts of accumulation and the effects of manganese and zinc on the seed germination and the growth of seedlings were investigated in Rumex crispus L. after Mn and Zn treatments. The effects of Mn and Zn on the germination rate in R. crispus L. showed 84∼89% in the treatments of the below 50ppm in both Mn and Zn, whereas it showed 65% in 100ppm Mn and 79% in 100ppm Zn, which had a relatively high germination rate in high concentration. The relative growth rate in R. crispus L. exhibited a tendency of somewhat restrained growth in the higher concentration, but showed a good growth compared to control. The relative growth rate of length in R. crispus L. as concentrations of Mn and Zn increase from 5ppm to 100ppm revealed a tendency of somewhat restrained growth, but showed a good growth compared to control. The accumulation amount of Mn and Zn in organs of R. crispus L. showed a tendency to increase in order of its stem < leaf < root, and the more time passed by after its treatment and the higher the concentrations of its treatment were, the more amount of their accumulation appeared. These results suggest that R. crispus L. is not only strong in tolerance in the contaminated soil of Mn and Zn but contributable to the purification of the contaminated soil.