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      • Coated tube를 사용한 CA19-9 측정용 IRMA 시약의 평가

        이현주,장현영,신선영,김희선,김태훈,이호영,Lee, Hyun-Ju,Jang, Hyun-Young,Shin, Sun-Young,Kim, Hee-Sun,Kim, Tae-Hoon,Lee, Ho-Young 대한핵의학기술학회 2010 핵의학 기술 Vol.14 No.2

        현재 본원은 TFB사의 CA19-9 항체가 bead에 코팅되어있는 CA19-9 IRMA 시약을 사용하고있다. 이번에 TFB사에서 새로 개발된 CA19-9 항체가 polystyrene test tube에 코팅된 CA 19-9 IRMA 시약이 기존 TFB사의 bead를 사용하는 시약을 대체하여 사용할 수 있는지 시약의 성능을 평가하였다. 본원과 서울대학교병원 환자 56명의 검체를 대상으로 하여 TFB사의 기존 bead 방법을 사용한 시약과 새로 개발된 coated tube를 사용한 시약을 비교하여 상관계수와 회귀식을 구하고 coated tube를 사용한 시약의 정밀도, 회수율, 직선성, 민감도, Hook effect 를 확인하였다. 저, 중, 고역가 검체의 검사내 정밀도는 4.1%, 4.0%, 4.2%이고 검사간 정밀도는 7.6%, 4.3%, 7.8%이며 회수율은 모두 $100{\pm}10%$이다. 직선성은 우수하였고 분석적민감도는 0.3U/mL이었으며 9,020,000 U/mL 농도에서는 Hook effect가 나타나지 않았다. 또한 TFB사의 기존 bead를 사용한 방법과 비교하여 상관관계를 분석한 결과, y=0.9185x-0.953, 상관계수 $R^2$=0.9779이었다. 이번 연구를 통해 본원은 TFB사에서 새로 개발된 coated tube CA19-9 IRMA 시약이 검사 시간과 검사 과정에서 간편화되고 검사시 자동화기기를 사용할 수 있으며 기존 bead를 사용한 시약에 대체하여 사용할 수 있다고 판단하였다. Purpose: $TFB^{(R)}$ CA19-9 IRMA kit uses beads coated with CA19-9 antibody. However, this mathod can not use automated equipment, and requires a long time test. Recently, CA19-9 IRMA kit developed by $TFB^{(R)}$ is coated with CA19-9 antibody to the polystyrene test tube and reaction at room temperature, and also reduced test time. This study evaluated the performance of a newly developed $TFB^{(R)}$ CA19-9 IRMA kit. Materials and Methods: This study were measured by using 56 patients sample of Boramae Medical Center and Seoul National University Hospital. We evaluated intra-and inter-assay precision, recovery rate, linearity, sensitivity and high dose hook effect of coated tube CA19-9 IRMA kit developed by $TFB^{(R)}$. The values of CA19-9 measured by $TFB^{(R)}$ bead kit were compared with those measured by $TFB^{(R)}$ coated tube kit. Results: ntra-assay coefficients of variation on three different levels were 4.1%, 4.0% and 4.2%. Inter-assay coefficients of variation were 7.6%, 4.3% and 7.8%. Recovery tests on all three different levels showed within $100{\pm}10%$. Linearity was good and sensitivity was 0.3 U/mL. High dose hook effect is not observed. There was strong correlation between bead kit and coated tube kit by $TFB^{(R)}$ CA19-9 IRMA kit. (y=0.9185x-0.953, $R^2$=0.9779) Conclusion: Coated tube CA19-9 IRMA kit developed by $TFB^{(R)}$ showed satisfactory precision, recovery rate, linearity, sensitivity and high dose hook effect.

      • KCI등재후보
      • KCI등재

        최신토지피복자료를 이용한 대구시의 열환경 수치모의

        이현주(Hyun-Ju Lee),이귀옥(Kwi Ok Lee1),원경미(Gyeong Mee Won),이화운(Hwa Woon Lee) 한국대기환경학회 2009 한국대기환경학회지 Vol.25 No.3

        The land surface precesses is very important to predict urban meteorological conditions. Thus, the latest land use data set to reflect the rapid progress in urbanization was applied to simulate urban thermal environment in Daegu. Because use of the U.S geological Survey (USGS) 25-category data, currently in the fifth-generation Pennsylvania State University-National Center for Atmospheric Research Mesoscale Model (MM5), does not accurately described the heterogeneity of urban surface, we replaced the land use data in USGS with the latest land-use data of the Korea Ministry of Environment over Daegu. The single urban category in existing 24-category U.S. Geological survey land cover classification used in MM5 was divided into 5 classes to account for heterogeneity of urban land cover. The new land cover classification (MC-LULC) improved the capability of MM5 to simulate the daytime part of the diurnal temperature cycle in the urban area. The ‘MC-LULC’ simulation produced the observed temperature field reasonably well, including spatial characteristics. The warm cores in western Daegu is characterized by an industrial area.

      • KCI등재

        가스 하이드레이트 형성 원리를 이용한 연소전 탈탄소화 연구

        이현주,이주동,김양도,Lee, Hyun-Ju,Lee, Ju-Dong,Kim, Yang-Do 한국재료학회 2008 한국재료학회지 Vol.18 No.12

        The emission of carbon dioxide from the burning of fossil fuels has been identified as a major contributor to green house emissions and subsequent global warming and climate changes. For these reasons, it is necessary to separate and recover $CO_2$ gas. A new process based on gas hydrate crystallization is proposed for the $CO_2$ separation/recovery of the gas mixture. In this study, gas hydrate from $CO_2/H_2$ gas mixtures was formed in a semi-batch stirred vessel at a constant pressure and temperature. This mixture is of interest to $CO_2$ separation and recovery in Integrated Coal Gasification (IGCC) plants. The impact of tetrahydrofuran (THF) on hydrate formation from the $CO_2/H_2$ was observed. The addition of THF not only reduced the equilibrium formation conditions significantly but also helped ease the formation of hydrates. This study illustrates the concept and provides the basic operations of the separation/recovery of $CO_2$ (pre-combustion capture) from a fuel gas ($CO_2/H_2$) mixture.

      • KCI등재
      • KCI등재

        『蓬萊酬唱錄』 (「附東萊府治圖」)에 관한 일고찰

        이현주 ( Lee Hyun-ju ) 동아시아문물연구학술재단 2016 文物硏究 Vol.- No.30

        『蓬萊酬唱錄』은 1725년 음력 3월 동래부사 趙錫命이 주관하여 여러 智人 들과 동래부에서 수창한 시문을 그림과 함께 장황한 총 35장의 서화첩이다. 서화첩 구성은 권두에 접위관 洪晟이 大字로 蓬萊, 酬唱이라 題名을 쓰고 다음 장에는 「東萊府治圖」를 배치하였으며, 다음으로 星州의 문사 鄭錫儒가 지은 <蓬萊酬唱錄序>가 있다. 그 뒤로 당시의 동래부사 조석명이 지은 책 제목 에 가늠하는 권두사에 해당되는 <題詞>를 두었고, 이어 본문에 해당되는 조석 명, 정석유, 洪晟, 趙駿命, 범어사 승려 存覺이 지은 시문 55수를 李仲寬이 행 서로 필사하였다. 卷末은 홍성이 짓고 쓴 <後記>가 장황되어 있다. 1725년(영조 1)년 당시의 동래부사 조석명은 나이가 52세로 이 벼슬을 마지막 으로 관직에서 물러날 뜻을 이미 갖고 있었는데, 여러 날에 걸친 酬唱詩를 서화 첩으로 묶어 후일 동래에서의 일을 回憶하고 破寂하기 위하여 제작한 것이며 또한 네 집안의 자제들이 뒷날 볼 수 있기를 희망하였다. 『봉래수창록』에서 특히 주목되는 것은「동래부치도」이다. 현재 알려진 대부분 의 동래부 관련 그림들이 후기동래읍성을 배경으로 제작된 반면 이 그림은 1731 년 정언섭의 후기읍성 축조 이전의 모습을 반영하고 있기 때문이다. 더욱이 묘사 된 관아시설과 주변지형의 공간배치는 상당히 정확하고 사실적이다. 묘사된 동래 부 내의 관아시설이 아주 상세하고 화풍에서도 18세기 동래부 무임화사계열의 화풍이 엿보이고 있을 뿐 아니라 그 그림의 주문자가 동래부사였다는 점을 감안 해볼 때 이 그림의 제작자는 동래부 무임화사일 가능성이 크다. 『봉래수창록』의 전반적인 내용에 관해서는 해제를 덧붙여 고찰하였다. 이 작품 은 18세기 동래지역에서의 수창교유와 시화첩의 제작배경을 확인할 수 있을 뿐 아니라 새로운 동래부 화사를 발굴할 중요한 사료이다. Bongraesuchangrok is a collection of poems and paintings containing 35 sheets of paper. It was arranged by Dongraebu Magistrate Seokmyeong Cho in March of the lunar calendar, 1725 and includes poetry with paintings the Magistrate and his acquaintances recited in Dongraebu. On the opening page of the collection are large characters, Bongrae and Suchang by Diplomat Seong Hong, which is followed by Dongraebuchido, preface by Seokyou Cheong from Seongju and J aesa, introductory remarks whose title is known by. In the body part of the collection, 55 poems by Seokmyeong Cho, Seokyou Cheong, Seong Hong, Junmyeong Cho and Monk Jonggak of Boemeo Temple were transcribed in the semicursive style of writing by JungGwan Lee. At the end was a postface by Seong Hong. In 1725 (King Youngjo 1st year), Dongraebu Magistrate Seokmyeong Cho was thinking of withdrawing from his office of Dongrae. He made the collection for recalling the experiences in Dongrae later and hoping that offsprings of 4 families involved would read it. Dongraebichido is especially noticeable in Bongraesuchangrok. While most known pictures of Dongraebu at this time are with the latter part settings of Dongrae Eupsung, this picture in Bongraesuchangrok reflects the time before EonSeop Cheong built the latter part settings of Dongrae Eupsung in 1731. Moreover, it was verified that governmental facilities and space arrangement in the picture are considerably accurate and realistic. There is a very strong possibility that the picture Dongraebuchido was produced by Muimhwasa in Dongraebu, given the fact that described governmental facilities are in great detail; the painting style of Muimwhasa in Dongraebu in the 18th century is shown; its orderer is Dongraebu Magistrate. This paper covers overall contents of Bongraesuchangrok with a bibliography. This collection is a very historical source not only for checking a poem recitation in Dongrae and the background of producing the collections of poems and paintings, but also for discovering new Dongrabuhwasa.

      • KCI등재후보

        한양방 협진 병원 종사자의 한의학 지식정도 및 교육요구도 평가

        이현주,김선림,정민수,최만규,Lee, Hyun-Ju,Kim, Sun-Lim,Jung, Min-Soo,Choi, Man-Kyu 대한예방한의학회 2008 대한예방한의학회지 Vol.12 No.1

        This study investigated the Oriental medicine knowledge and educational requirement of medical staff working in Oriental-Western collaborative medicine hospitals(except for Oriental and Western medicine doctors) based on the recognition that not only mutual understanding and cooperation between Oriental and Western medicine doctors but also the knowledge of Oriental medicine of medical support staff such as nurses, medical technologists, pharmacists and administrative staff are very important to promote Oriental-Western collaborative medical treatment. The study results are summarized as follows : First, it was found that the ratio of nurses who took Oriental medicine education was much higher than those of other groups. They took Oriental medicine education in the types of school curriculum (27.0%) and special lectures in workplace(20.4%). Second, many of the people who took Oriental medicine education were found to be not satisfied with the education in general - 32.7% of them answered the education content was "so so" and 48.4% of them answered "unsatisfactory." Third, the general necessity of Oriental medicine education was found to be an average of 3.60 out of 5, and the number was higher "after employment"(average=3.85) than "before employment"(average=3.04). Fourth, the study found that Oriental-Western collaborative medicine hospital staff are well aware of the necessity of the knowledge of Oriental medicine in the cases of communications between different occupational types, consultations with patients or their guardians, treatment and nursing and the establishment of the practice of specialized Oriental medicine institutes. Fifth, the levels of Oriental medicine knowledge showed a difference in average value according to the role range(p<0.000), and it was found that there is an interaction effect between occupation type and role range(p<0.015).

      • KCI등재

        서울지역 공공의료기관 간호사의 병동과 병동 외 구분에 따른 직무스트레스와 피로

        이현주,Lee, Hyun-Ju 한국학교ㆍ지역보건교육학회 2018 한국학교지역보건교육학회지 Vol.19 No.1

        Objectives: The study was conducted to understand job stress and fatigue conditions by dividing nurses in a polyclinic-level public medical institution, Seoul with more than 600 beds into ward nurses and non-ward nurses and to comprehend sub-areas of job stress that affect fatigue. Methods: A survey was conducted from August 18 2014 to September 12 2014, so 216 cases were analyzed by using PASW statistics 18.0. Results: Job stress of ward nurses is significantly high in the psychological burden of nursing service area and medical limit. Fatigue of ward nurses is also higher. As a result of multiple Linear regression, nursing service area affects fatigue of ward nurses and there is no significant influence factors in non-ward nurses. Conclusion: Therefore, mental health education and interest of hospital in nursing service area are more needed for ward nurses with high job stress and fatigue among nurses.

      • 팔꿉관절과 손목관절 각도가 쥐는 힘과 집는 힘에 미치는 영향

        이현주,이승주,Lee, Hyun-Ju,Yi, Seung-Ju 대한물리치료학회 2003 대한물리치료학회지 Vol.15 No.4

        The purpose of this study were to determine the effect on grip and pinch strength with elbow and wrist angle change. 112 college students, 88 males, and 24 females aged 19 to 34 years, participated in the study. A Grip and pinch strength was measured in two elbow position($0^{\circ}$ and $90^{\circ}$) and three wrist position($80^{\circ},\;0^{\circ},\;23^{\circ}$). The data were analyzed by mean and deviation, and t-test using the PC/SAS system. These results were obtained as follows; 1. There was a more strength grip and pinch power in $0^{\circ}$ than $90^{\circ}$ elbow flexion at three wrist angle. 2. There was a significant high grip and pinch strength in $23^{\circ}$ dorsiflexion among three wrist angle(p<0.01). 3. The grip and pinch strength power was measured higher in male than female every elbow and wrist angle(p<0.01).

      • 자동분주기의 이월오염 평가

        이현주,민경선,신선영,우재룡,이호영,Lee, Hyun-Ju,Min, Gyung-Sun,Shin, Sun-Young,Woo, Jae-Ryong,Lee, Ho-Young 대한핵의학기술학회 2009 핵의학 기술 Vol.13 No.3

        목적 : 자동분주기의 fixed probe pipetting system 은 검체 분주시 probe 내외부의 잔여물이 다음 pipetting cycle로 이월되어 처음 측정치가 차기 측정치의 결과에 영향을 미치는 이월오염(carryover contamination)을 발생시킬 수 있다. 이번 연구는 본원에 도입된 TECAN 사의 Freedom Evo 100 자동 분주기의 $\alpha$-fetoprotein (AFP)과 carcinoembryonic antigen (CEA) 검사 시 검체간 이월오염 발생을 확인하였다. 방법 : reference 가 되는 오염이 안 된 저농도 검체를 연속분주한다. 그런 후 고농도 검체를 분주한 다음 오염이 예상되는 저농도 검체를 연속 3회 분주하는 과정을 교대로 반복한다. 오염이 안 된 저농도 검체와 오염이 예상되는 저농도 검체값의 각 평균값을 서로 비교하여보고 각 저농도 검체값이 최소 검출 농도 이하인지 확인하고 만약 저농도 검체값간의 평균값이 차이가 있다면 그 차이가 1 ppm 이하의 carryover target 농도값 이하인지 확인한다. 결과 : AFP 약 650,000 IU/mL와 CEA 약 65,000 ng/mL의 고농도 검체에서는 오염이 안 된 저농도 검체값과 오염이 예상되는 저농도 검체값의 평균값 모두 각 검사의 최소 검출 농도값 이하로 이월오염이 나타나지 않았다. 결론 : 이번 연구에서 사용한 AFP와 CEA의 고농도 검체에서는 이월오염이 나타나지 않았지만 검사자는 검사결과 보고시 고농도 검체가 다음 환자 검사결과의 안정성에 영향을 줄 수 있음을 주시하고 이월오염을 확인하기위해 주기적인 장비관리와 이월오염 측정을 시행하여야한다. Purpose: Autopipetting system is an efficient automated equipment pipetting patient samples and reagents for rapid and accurate test. However, it can cause carryover between high concentration sample and low concentration sample. We evaluated carryover contamination of TECAN freedom Evo 100 autopipetting system. Materials and Method: We studied carryover contamination of $\alpha$-fetoprotein (AFP) and carcinoembryonic antigen (CEA) test on TECAN freedom Evo 100 autopipetting system. Very low concentration control samples were pipetted for comparison to the contaminated very low concentration samples. Then, The contaminated very low concentration samples were pipetted following the high concentration samples were pipetted alternately. The difference of low concentration samples represents carryover. The target value to decide carryover was 1ppm (parts per million). Results: For AFP, the mean values of the uncontaminated control samples and the contaminated samples were less than 0.6 IU/mL (the l imit of detection (LoD)). Carryover did not occur even though the high concentration sample which value was 650000 IU/mL. For CEA, the values of the low concentration control samples and the contaminated samples were less than 0.2 ng/mL (LoD). Carryover did not occur even though the high concentration sample which value was 65,000 ng/mL. Conclusions: Sample carryover was not found on TECAN freedom Evo 100 autopipetting system for AFP, CEA. However, carryover is a potential problem with automated instruments and robotic pipetting systems. Therefore, Clinical laboratories must periodically verify carryover contamination for the accurate and confidential test results.

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