http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 이진 ( Gene Lee ) (검색결과 5 건)
- 무료
- 기관 내 무료
- 유료
-
-
유형근,신형식,이진,민병무,You, Hyung-Keun,Shin, Hyung-Shik,Lee, Gene,Min, Byung-Moo The Korean Academy of Periodontoloy 1999 Journal of Periodontal & Implant Science Vol.29 No.3
치주인대세포는 치주인대의 유지와 개조에 있어서 중요한 역할을 담당하는 섬유아세포성 세포로서, 세포에 가해진 여러가지 조건에 따라 다양한 표현형의 변화를 나타내는 것으로 알려져 있다. 기계적 응력은 치주인대세포의 세포증 식과 밀접히 연관되어 있는 것으로 알려져 있으며, 이는 세포주기 조절인자들의 발현을 증가 시킴으로써 이루어질 것으로 생각되나 그 자세 한 작용기전은 알려져 있지 않다. 그러므로 이 연구의 목적은 기계적 응력이 사람 치주인대세 포의 세포증식과 세포주기 조절인자의 발현에 미치는 영향을 연구하기 위하여 사람 치주인대 세포에 기계적 응력을 가한 후 세포증식을 관찰하고 , 세포주기조절인자들인 p 53 , $p21^{WAF1/CIP1}$ cyclin-dependent kinases(cdks), cyclins 및 proliferating cell nuclear antigen(PCNA)의 단백질 발현 변화를 연구하였다. 본 연구에 사용한 사람 치주인 대세포는 교정치료를 목적으로 발거한 건전한 사람 소구치의 치주인대로부터 explantation culture하여 얻은 후 계대배양을 시행하여 제6 계대의 세포를 사용하였다. 배양한 사람 치주인 대세포를 55-mm Petriperm dish당 $1{\times}10^4$ 개를 분주하고, dish당 1kg의 기계적 응력을 가하면서 12일동안 세포배양을 시행하였다. 사람 치주인대세포의 세포증식은 기계적 응력을 가한 후 8-12일 사이에 현저히 증가하였으며, PCNA 단백질의 발현은 기계적 응력을 가한 후 6-10일 사이에 현저히 증가하였다. 또한 기계적 응력은 사람 치주 대세포의 cdk4, cdk6, cdk2 및 cyclin D1 단백질의 발현을 다소 증가 시켰으나, p53 및 $p21^{WAF1/CIP1}$ 단백질의 발현은 큰 변화가 없었다. 이상의 결과 서 기계적 응력은 사람 치주인대세포 의 p53 및 $p21^{WAF1/CIP1}$ 단백질 발현의 변화 없이 cdks 단백질 발현을 증가시킴으로써 세포증식을 증가시키는 것으로 생각된다.
-
-
흰쥐 두개관 및 간에서 Purine이화대사효소 및 회수경로효소의 활성에 관한 연구
이진,이종흔 대한구강생물학회 1989 International Journal of Oral Biology Vol.13 No.2
As an attempt to clarify one end of the metabolic fate of purine bases, the activities of several enzymes involved in it were assayed in rat calvaria and liver. The portions of calvarias containing frontal and parietal bones and liver were separated from rats weighing 200±20gm. Specimens were assayed for their activities of guanine aminohydrolase and xanthine oxidase in its cytosol and HGPRT and APRT in its supernatant of mitochondrial fraction. Specific activities of guanine aminohydrolase and xanthine oxidase with hypoxanthine or xanthine as substrate in calvaria were 1.84 and 3.34 or 4.55, respectively. Guanine aminohydrolase and xanthine oxidase in liver, however, had higher than calvaria. Specific activities of HGPRT with guanine or hypoxanthine as substrate and APRT in calvaria were 27.01 or 30.66 and 3.32, respectively. But HGPRT and APRT in liver had lower than calvaria. Taken together, these results show that in calvaria most purine bases would be reutilized by salvage enzymes rather than completely catabolized but both of catabolic pathway to final metabolites and salvage pathway for purines would be active as well as de novo synthesis in liver. Therefore metabolic fate of purine bases varied from tissue to tissue within same species.
-
김관식,민병무,김세원,이진 대한구강생물학회 1989 International Journal of Oral Biology Vol.13 No.1
As an attempt to clarify the metabolic fate of purine bases in rat calvaria and liver, the activities of xanthine oxidase, guanine aminohydrolase and HGPRT were assayed. The portions of calvaria containing frontal and parietal bone, and liver were dissected from rats. Specimens were assayed for their activities of xanthine oxidase and guanine aminohydrolase in its cytosol and HGPRT in its mitochondrial fraction. Specific activity of hepatic xanthine oxidase with hypoxanthine or xanthine as its substrate was 4.18 or 7.43mU/mg of protein and guanine aminohydrolase was 11.00mU/mg of protein, respectively. In contrast, calvarial xanthine oxidase and guanine aminohydrolase was 1.49 or 1.88 and 0.98, respectively, which were significantly lower than those of hepatic enzymes. Specific activity of calvarial HGPRT was 21.70 and hepatic HGPRT was 13.65mU/mg of protein when hypoxanthine was employed as its substrate while no measurable activity was detected in calvaria when guanine was employed. Comparing to purine-catabolizing enzymes, activity of HGPRT, salvage-enzyme for purine bases, was relatively higher in both tissues and hypoxanthine was utilized preferentially as substrate than guanine. Besides the production of GMP or IMP by HGPRT, concentration of other purine nucleotides such as GMP from guanine or adenylates form hypoxanthine were also increased in hepatic HGPRT assay but no detectable changes in calvaria. Assuming these increments as the consequences of HGPRT activity, it can be deduced that activity of HGPRT in liver would be greater than that of calvaria. Taken together, these results indicated thet ⅰ) in calvaria, most of purine bases would be reutilized by salvage enzymes rather than completely catabolized ⅱ) both of catabolic pathway to final metabolites and salvage pathway for purines would be active as well as de novo synthesis in liver ⅲ) metabolic fate of purine bases varied from tissue to tissue within same species.
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
