해양퇴적물의 핵산추출물에서 humic substances의 효율적인 제거방법

이정현,신현희,이홍금,권개경,김상진,Lee, Jung-Hyun,Shin, Hyun-Hee,Lee, HongKun,Kwon, Kae Kyoung,Ki, Sang-Jin 한국미생물학회 1998 미생물학회지 Vol.34 No.3

자연환경의 미생물군집을 분자생태학적 방법으로 분석하기 위해서는 오염되지 않은 순수한 핵산의 분리가 필요하다. 해양퇴적물에서 핵산을 추출할 때 같이 추출되는 부식물질(humic substances)은 핵산중폭반응을 저해하기 때문에, 이를 제거하기 위한 네 가지의 방법, 아가로즈젤 전기영동후 용출, Sephadex G-75 젤, Hydroxyapatite 젤, 그리고 PVPP(poluvinylpolypyrrolidone) microspin column 방법에 대해 그 효율성을 비교하였다. 조산된 방법 중에서 PVPP microspin column을 이용하면 건조 퇴적물 1g당 $4.8{\mu}g$의 순수한 DNA를 신속하고 간편하게 얻을 수 있었고, 이 방법으로 분리된 DNA를 PCR의 주형으로 사용한 결과 16S rRNA 유전자의 거의 전 범위에 해당하는 1.5 kb 크기의 PCR 산물을 얻을 수 있었다. Molecular analyses of natural microbial communities are often dependent upon the obtainments of pure nucleic acids. The four methods (elution after agarose gel electrophoresis, G-75 microspin columns, hydroxyapatite mi-crospin columns, and polyvinylpolypyrrolidone (PVPP) microspin columns) were compared for the removal of PCR-inhibitory humic substances from the crude DNA extracts of marine sediment samples. The PVPP microspin columns have shown superior removal of humic substances from the crude DNA extract of marine sediment samples, with yield of $4.8{\mu}g/g$ (dry weight of sediment). The purified DNA by this rapid method was pure enough to amplify 1.5 kb fragment corresponding almost full length of 16S rRNA genes.

체외수정 및 배아이식술에서 레이저를 이용한 보조부화술의 효용성에 대한 연구

이정현,한지은,김유신,원형재,조정현,곽인평,엄진희,박은아,최윤정,이동률,윤태기,Lee, Jung-Hyun,Han, Ji-Eun,Kim, You-Shin,Won, Hyung-Jae,Cho, Chung-Hyun,Kwak, In-Pyung,Eum, Jin-Hee,Park, Eun-A,Choi, Yoon-Jung,Lee, Dong-Ryul,Yoon, Tae-Ki 대한생식의학회 2008 Clinical and Experimental Reproductive Medicine Vol.35 No.3

목 적: 보조부화술이 적용되는 좋지 않은 예후를 보여주는 선별된 환자군을 대상으로 산성 용액을 이용한 AHA 방법과 레이저를 이용한 AHL 방법의 효용성을 비교하여, 보다 효과적으로 임신율과 착상률을 높일 수 있는 보조부화술 방법을 찾고자 하였다. 연구방법: 2006년 2월부터 9월까지 체외수정 시술을 시행한 환자 중 보조부화술이 필요한 328주기를 대상으로 산성용액을 이용한 AHA 방법 (180주기)과 ZILOS-tk 레이저를 이용한 AHL 방법 (148주기)으로 나누어 시행하였다. 보조부화술을 시행한 환자군은 환자의 나이가 38세 이상이거나 투명대의 두께가 $18{\mu}m$ 이상, 기저 FSH 농도가 15 mIU/ml이상, 체외수정 시술을 3번 이상 실패한 환자, 이식하는 배아의 상태가 양호하지 않은 환자들로 이상에 적용요인이 있으면 시행하여 무작위로 보조부화술 방법간에 환자들의 임상적 특징과 임신율과 착상률을 분석하였다. 결 과: 전체 보조부화술을 시행한 환자군에 AHL 방법과 AHA 방법간에 임신율 (42.6%, 63/148 vs. 33.3%, 60/180)과 착상률 (17.4%, 82/470 vs. 16.0%, 89/556)에 유의적 차이는 나타나지 않았다. 그러나 나이가 많은 환자군인 Group 1은 임신율 (37.0%, 20/54 vs. 18.7%, 14/75)과 착상률 (14.4%, 23/160 vs. 7.1%, 15/210)이 AHL 방법이 AHA 방법보다 유의적 (p<0.05)으로 높게 나타났다. 전체 환자군이나 선별된 각 군내에 보조부화술 방법간에 환자의 임상적 특징은 유의적 차이가 나타나지 않았다. 3번 이상 체외시술에 실패한 환자군 [Group 2: 43.8% (21/48)과 31.6% (25/79)], 투명대의 두께가 $18{\mu}m$ 이상인 환자군 [Group 3: 43.8% (32/73)과 34.1% (28/82)], 이식한 배아의 질이 양호하지 않은 환자군[Group 4: 25.0% (7/28)과 14.6% (6/41)]에서는 AHL 방법이 임상결과는 좋았으나 유의적 차이는 없었다. 결 론: 레이저를 이용한 AHL 방법이 나이가 많은 환자군과 3번 이상 체외수정 시술에 실패한 환자군에서 AHA방법에 비해 높은 임신율과 착상률을 나타내었다. 결론적으로, AHL을 이용한 보조부화술이 임상적으로 보다 효과적이고 안전한 방법이라고 사료된다. Objective: To evaluate efficacy of assisted hatching by laser (AHL) and acidified Tyrode solution (AHA) in selected groups of IVF-ET patients who have a poor prognosis. Methods: From February 2006 to September 2006, total of 328 IVF-ET cycles with advanced female age (${\geq}38$ years), recurrent implantation failure (${\geq}3$ cycles), thick zona (${\geq}17{\mu}m$), and/or poor quality of embryo were randomly divided into assisted hatching by acidified Tyrode solution (AHA, n=180) and the assisted hatching using the ZILOS-tk laser (AHL, n=148) groups. Clinical outcomes were analyzed and compared between AHA and AHL group based on the patient characteristics. Results: In all AHL and AHA group, there were no significant differences in pregnancy (42.6%, 63/148 vs. 33.3%, 60/180) and implantation rates (17.4%, 82/470 vs. 16.0%, 89/556) However, in advanced female age group (Group 1), pregnancy (37.0%, 20/54 vs. 18.7%, 14/75) and implantation rates (14.4%, 23/160 vs. 7.1%, 15/210) in AHL group were significantly (p<0.05) higher than those of AHA, although there was no difference in patient parameters of both groups. And, the clinical outcome of groups with recurrent implantation failure (Group 2), thick zona pellucida (Group 3) and poor quality embryo (Group 4) were improved in AHL compared to those of AHA: 43.8% (21/48) and 31.6% (25/79) in Group 2, 43.8% (32/73) and 34.1% (28/82) in Group 3, 25.0% (7/28) and 14.6% (6/41) in Group 4, but no significance. Conclusion: The AHL improved the pregnancy and implantation rates in patients with advanced female age and recurrent implantation failure when compared to outcomes achieved from AHA. Therefore, this AHL technique may be a efficient and safe method for patients with poor prognosis.

무한사면모델과 수리학적 모델의 결합을 통한 강원도 진부지역의 산사태 취약성 분석

이정현,박혁진,Lee, Jung Hyun,Park, Hyuck Jin 대한자원환경지질학회 2012 자원환경지질 Vol.45 No.6

정량적인 산사태 취약성 분석은 산사태를 유발하는 인자 및 모델에 대한 접근방법에 따라 통계적 기법과 지질역학적 기법으로 구분된다. 이 중 지질역학적 기법은 산사태 모델을 가정하고 사면의 기하학적 특성과 사면 구성물질의 공학적 특성을 고려하여 산사태의 취약성을 판단하는 기법으로 산사태의 발생메커니즘과 과정을 고려할 수 있다는 장점을 가지고 있어 산사태의 취약성 분석에 가장 효과적인 기법 중의 하나로 보고되고 있다. 지질역학적 해석기법의 경우 최근 들어 무한사면모델이 주로 사면 모델로 사용되고 있으며 GIS의 활용을 통해 광역적인 지역에 대한 분석이 가능해짐에 따라 무한사면모델을 이용한 광역적인 지역에서의 산사태 취약성 분석이 가능해졌다. 기존의 무한사면모델을 활용한 연구의 경우 연구지역의 지하수위를 지반이나 강우의 특성에 대한 고려 없이 임의로 가정하여 해석함으로써 강우량과 연구지역의 지반특성에 따라 지하수위가 유동적으로 포화되는 것을 전혀 고려할 수 없는 문제점을 가지고 있다. 본 연구에서는 이를 보완하기 위해 산사태의 유발에 가장 큰 영향을 미치는 강우강도와 지반의 수리특성을 반영할 수 있는 수리학적 모델을 무한사면모델과 결합하여 연구지역의 현장 조건을 반영한 산사태 취약성 분석을 수행하였다. 또한 기존의 해석방법과 본 연구에서 제안된 해석기법을 비교분석하기 위하여 2006년 7월 대규모의 산사태가 발생한 강원도 진부지역을 대상으로 분석을 수행하였다. 그 결과 본 연구에서 제안된 해석기법이 기존의 해석기법에 비해 높은 예측 정확도를 보이는 것으로 분석되었다. The quantitative landslide susceptibility assessment methods can be divided into statistical approaches and geomechanical approaches based on the consideration of the triggering factors and landslide models. The geomechanical approach is considered as one of the most effective approaches since this approach proposes physical slope model and considers geomorphological and geomechanical properties of slope materials. Therefore, the geomechanical approaches has been used widely in landslide susceptibility analysis using the infinite slope model as physical slope model. However, the previous studies assumed constant groundwater level for broad study area without the consideration of rainfall intensity and hydraulic properties of soil materials. Therefore, in this study, landslide susceptibility assessment was implemented using the coupled infinite slope model with hydrologic model. For the analysis, geomechanical and hydrualic properties of slope materials and rainfall intensity were measured from the soil samples which were obtained from field investigation. For the practical application, the proposed approach was applied to Jinbu area, Gangwon-Do which was experienced large amount of landslides in July 2006. In order to compare to the proposed approach, the previous approach was used to analyze the landslide susceptibility using randomly selected groundwater level. Comparison of the results shows that the accuracy of the proposed method was improved with the consideration of the hydrologic model.

V형 아형 간헐외사시의 수술적 치료

이정현,정재호,최희영,Jung Hyun Lee,Jae Ho Jung,Hee Young Choi 대한안과학회 2011 대한안과학회지 Vol.52 No.7

Purpose: To compare the surgical methods for the treatment of sub-V pattern intermittent exotropia. Methods: Sub-V pattern intermittent exotropia is defined as 8-15 prism diopters (PD) less horizontal deviation in downgaze than in upgaze. The present study included 65 patients who underwent a standard recession procedure of the lateral rectus muscle (group I), oblique muscle surgery (group II) or vertical transposition of the horizontal rectus muscle (group III) and who were followed up for six months after the surgery. Results: Surgical success was defined as vertical incomitance ≤5 PD; the success rate was 92% (23/25) in group I, 88.8% (16/18) in group II and 86.4% (19/22) in group III. Surgical success of the horizontal strabismus was defined as horizontal deviation ≤10 PD at primary position; the success rate was 87.9% (51/58) in the patients with vertical incomitance less than 5 PD, and 42.9% (3/7) in the patients with vertical incomitance greater than 6 PD. There was statistical significance in the success rate of horizontal strabismus according to the decrease in vertical incomitance (p=0.01). Conclusions: There was no statistical significance of the difference in success rates of horizontal strabismus surgery regardless of vertical transposition of the horizontal rectus muscle or oblique muscle surgery. There was statistical significance in the difference in success rates of horizontal strabismus according to the reduction in vertical incomitance. J Korean Ophthalmol Soc 2011;52(7):846-851

암 환자에서 우울증의 선별도구로서 단축형 에딘버러 우울증 척도의 한국 표준화

이정현,김태석,고윤호,윤수정,류인균,전태연,이철,Lee, Jung Hyun,Kim, Tae-Suk,Ko, Yoon-Ho,Yoon, Sujung J.,Lyoo, In Kyoon,Jun, Tae-Youn,Lee, Chul 대한생물정신의학회 2009 생물정신의학 Vol.16 No.2

Objectives : Depression is a common psychiatric disorder in cancer patients. The Brief Edinburgh Depression Scale(BEDS), which is an abbreviated version of the Edinburgh Depression Scale, may serve as a useful tool in screening for the depression in patients with the medical illnesses. This report investigated the reliability and validity of the Korean Version of the BEDS(K-BEDS) for the depression in cancer patients. Methods : One-hundred cancer patients were enrolled in this study. All subjects completed the K-BEDS, the Hospital Anxiety Depression Scale(HADS), and the Karnofsky Performance Status Scale(KPSS). Reliability, validity and Receiver Operating Characteristic(ROC) curve analysis measures were assessed. Results : The K-BEDS showed good internal consistency(Cronbach ${\alpha}$=0.77) and test-retest reliability(0.94, p<0.001). All item-total correlations were above 0.3. Also, it revealed moderate correlation with the depression subscale of the HADS(r=0.617), but no correlation with the KPSS. Exploratory factor analysis produced only one factor, accounting for 47.1% of the total variance. The most valid cutoff value to screen for depression was a total score of 5 on the K-BEDS, which showed sensitivity of 62.5% and specificity of 86.4% with a positive predictive value of 4.60 and a negative predictive value of 0.43.

감성 디자인이 적용된 자동차 인테리어 디자인을 위한 인스트루먼트 패널 초음파 용접에 관한 연구

이정현,Lee, Jung-Hyun 한국생산제조학회 2010 한국생산제조학회지 Vol.26 No.2

Today's consumers are looking for emotional design which can fulfill their own potential desire. Emotion varies according to individual circumstance, age, sex, culture, education, profession and so on. Automotive instrument panel design is the most important part of interior design, because it affects the impression of interior design and has the equipments for safety, entertainment and various information. Thus, this study was performed to apply emotional design to automotive instrument panel which is the most important part of automotive interior, and find the best bonding conditions to build instrument panel efficiently by comparing mechanical properties in thermoplastic resin of polyethylene (PE) adhesion. Satisfactory adhesion was executed in ultrasonic welding for the same materials of PE. The best welding conditions were found to be welding time of I second, welding pressure of 250 kPA for PE-PE welding. Dissimilar materials were adhered when adhesion and ultrasonic welding were performed at the same time.

이침자극(耳鍼刺戟)이 절식(絶食) Stress로 인(因)한 흰쥐 대뇌피질(大腦皮質)의 NADPH-diaphorase 신경세포(神經細胞)에 미치는 영향(影響)

이정현,김이화,이은용,Lee, Jung-hyun,Kim, Ee-Hwa,Lee, Eun-yong 대한침구의학회 2001 대한침구의학회지 Vol.18 No.2

Objective : The aim of the present study is to investigate whether stimulating a specific auricular point(胃點) is effect on suppression of appetite. Methods : This study evaluated the changes of Reduced-Nicotinamide-Adenine-inucieotide-Phosphate-diaphorase(NADPH-d)-positive neurons using a histoehemical method. Staining intensities of NADPH-d-positive neurons were assessed in a quantitative fashion using a microdensitometrical method based on optical density by means of an image analyzer. Results : The results show as follows ; 1. In the RSG/RSA, FRI/FR2, HL, PAR1 area, The optical density of NADPH-d-positive neurons of fasted group was significantly decreased in comparison to fed group. 2. In most cortical area, It was not significant statistically, But the optical density of NADPH-d-positive neurons of fed with auricular acupuncture group was decreased in comparison to fed group. 3. In most cortical area, It was not significant statistically, But fasted with auricular acupuncture group increased the optical density of NADPH-d-positive neurons comparison to fasted group. Conclusions : We conclude that stimulating the specific auricular points is able to effect on NO(Nitric Oxide)-expression in the brain cortex of sprague dawley rats, The results suggest that auricular acupuncture may be useful for controlling obesity.

레이어 개념을 이용한 자동차 헤드램프 디자인과 이종재료 접합을 통한 시제품 제작에 관한 연구

이정현,Lee, Jung-Hyun 한국생산제조학회 2010 한국생산제조학회지 Vol.25 No.3

Decreasing products lifecycles and increasing consumers desires for quality and design make the automotive industries try to reduce time for developing new designs. In order to reduce developing time, I have designed head lamps, which are really important to have an effect on brand identities and images, using layers concept that is one of the international automotive design trends by alias and photoshop, and produced prototype by RP. To assemble the produced prototype to the body and manufacture the frame to exterior modelling efficiently, I have studied on joining dissimilar materials of aluminum alloy that can make the prototype lighter and stainless steel, which is good for corrosion resistance by using laser beam. These materials were welded for finding the optimum joining condition and evaluating the soundness of joining zone. The joining was performed under the condition of laser power 500, 550, 575, 600W and 11~14Hz. In this study, the suitable joining condition between aluminum alloy (Al 2024) and stainless steel (STS 304) can be obtained at the laser power 575W and frequency 12Hz.

요통환자(腰痛患者)의 양도락(良導絡) 변화(變化)에 대한 임상적(臨床的) 고찰(考察)

이정현,김민수,이은용,Lee, Jung-hyun,Kim, Min-soo,Lee, Eun-yong 대한침구의학회 2003 대한침구의학회지 Vol.20 No.1

Objective : We investigated association between excessiveness and deficiency of the visceral and twelve merdians and low back pain, by checking Yangdorak. Methods : Clinical studies were done 62 patients who were treated with low back pain to Dept. of Acupuncture & Moxibustion, Hospital of Oriental Medicine in Semyung University from August 2, 2002 to August 20, 2002. We divided low back pain patients into lumbar vertebra strain, herniated nucleous pulposus(H.N.P.) degenerative spondylosis(D.J.D) and tested the potentiality of skin resistance(Yangdorak) to them. Results: 1. H.N.P. groups were more than another groups in comparing with the States over Physiological Limits and the and excessiveness of merdians. 2. In degenerative spondylosis groups, excessiveness of the F3(kidney) was to be superior. In H.N.P. groups deficiency of the H5(triple energiger) was to be superior. In lumbar vertebra strain groups, deficiency of the H5(triple energiger) and excessiveness of the F2(Liver) was to be very superior. Conclusions: We could investigate the relationship of the excessiveness and deficiency of the visceral and twelve merdians to low back spain patients by checking Yangdorak. Specially, Deficiency of the H5(triple energiger) and H4(Small intestine) may be helpful in diagnosis H.N.P..

보행장애를 주소로 4세에 진단된 프로피온산혈증

이정현,고정민,유한욱,Lee, Jung Hyun,Ko, Jung Min,Yoo, Han-Wook 대한유전성대사질환학회 2006 대한유전성대사질환학회지 Vol.6 No.1

Propionic acidemia is an autosomal recessive metabolic disorder caused by a defect of propionyl CoA carboxylase with resultant accumulation of toxic organic acid metabolites. This disorder is biochemically characterized by metabolic acidosis, ketoacidosis, hyperglycinemia and hyperammonemia. Clinical symptoms are very heterogeneous and present as a severe neonatal-onset or a late-onet form. We describe one case of propionic acidemia in a 4-year-old boy who has developed gait disturbance after acute metabolic decompensation.