http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이정순,손건호,권정숙,이용우,권태완,김정상 한국콩연구회 2003 韓國콩硏究會誌 Vol.20 No.1
Soybean has drawn much attention due to its potential to prevent breast and prostate cancers as well as prevention of cardiovascular disease and osteoporosis. The previous study in this laboratory showed that whole soybean flour and semi-purified isoflavones inhibited benzo(a)pyrene-induced lung and stomach cancers. To address the possible mechanism of chemopreventive activity of soybean the ability of soy extract and isoflavones to activate cellular differentiation significantly. Hexane, ethylacetate, butanol and water-soluble fractions induced cell differentiation by 23, 44, 19 and 17%, respectively. Silica-gel chromatography produced 12 subfractions from ethylacetate-soluble fraction. Among them two fractions including subfraction 7 and 8 showed about 40% induction of differentiation of HL-60 cells at the concentration of 20 ㎍/㎖ and strong anti-proliferative activity at the concentration of 20㎍/㎖ against MCF-7 cells. Further fractionation of subfraction 7 and 8 generated nine sub-subfractions, respectively. Cancer cell differentiation by four sub-fractions(#7-1, # 7-5, #7-8, #7-9) from subfraction 7 was achieved by 12 to 28%. The sub-fraction 4 from subfraction 8 (#8-4) induced HL-60 cell differentiation by 41% at the concentration of 10 ㎍/mL, and showed a potent growth-inhibitory activity, with IC_(50) of and 11 ug/ml against HL-60 cells and MCF-7 cells, respectively. Meanwhile, #8-4 turned out to contain 25% daidzein as evaluated by NMR and HPLC-mass. However, components other than daizein must by responsible for the growth inhibition and differentiation of cancer cells as daidzein did not show cytotoxicity or cancer cell differentiating activity at the effective dose of #8-4. In conclusion, #8-4 merits further purification and characterization as it showed a great potential as a cancer preventive agent.