돌아온 겨울 철새 AI 방역 비상

이수연,Lee, Su-Yeon 한국오리협회 2007 오리마을 Vol.54 No.-

Heterogeneous expression of Chlamydia pneumoniae antigen candidates and high-level soluble expression of its inclusion membrane proteins in Escherichia coli

이수연,Sunjin Lee,Ji-Young Ahn,Jung Ho Ko,Lyon Lee,Janet Han,Young-Chang Kim,Yang-Hoon Kim 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.4

Chlamydia pneumoniae is an intracellular bacterium associated with several acute respiratory diseases and is an obligate intracellular parasite in eukaryotic cells. Heterogeneous and overexpression of virulence-associated C. pneumoniae “effector” proteins with structurally designed epitope-focused antigens in recombinant forms in Escherichia coli is a prerequisite in the study of the organism’s pathogenic mechanisms and for use in medical applications such as vaccine and therapeutics development. In this study, seven antigen candidates from C. pneumoniae TW-183 (OmpH, Omp11, Eno, ArtJ, IncA, IncB and IncC) were screened in silico genome. Immunogenicity of the heterogeneously overexpressed antigen candidates in E. coli BL21 (DE3) was investigated by Western blot analysis using in sera from C. pneumoniae infected individuals. The soluble forms of three inclusion membrane proteins (IncA, IncB and IncC), carrying unique bilobal hydrophobic domains, were also successfully expressed in E. coli BL21 (DE3) through truncation of their membrane bound transmembrane domains.

Proteomic analysis of muscarinic acetylcholine receptor-mediated proliferation in HT-29 human colon cancer cells

이수연,이아름,안지영,Jung Ho Ko,Lyon Lee,Janet Han,Young-Chang Kim,Yang-Hoon Kim 대한독성 유전단백체 학회 2018 Molecular & cellular toxicology Vol.14 No.2

Backgrounds: Muscarinic acetylcholine receptors (mAChRs) are members of G-protein-coupled receptors. They can induce agonist-dependent neoplastic transformation and facilitate colon cancer proliferation via promoting rapid expression of a variety of early responsive genes. Methods: In this study, we used 2-dimensional gel electrophoresis(2-DE) approach with subsequent mass spectrometry (MS) to identify up- and down-regulated proteins (a total of 23 protein spots) involved in mAChRs-related signaling pathway, energy metabolism, transcription/translation, oxidative stress metabolism and cytoskeleton organization in agonist carbachol stimulated HT-29 human colon cells. Results: We found that the increased expression of adenocarcinoma biomarker, annexin A5 (ANXA5) induced by carbachol treatment, which was confirmed by immunoblot. This study contributes to the understanding of mechanisms underlying mAChRs agonist-induced expression of whole proteins in HT-29 colon cancer cells. Conclusion: Our results indicated that ANXA5 might serve as a potential biomarker for the diagnosis of colon cancer.

신반흔 예측인자로서 초기 $^{99m}Technetium-dimercaptosuccinic\;Acid$ 신주사 소견과 방광요관 역류의 유무 및 정도

이수연,임소희,이대열,Lee Soo-Yeon,Lim So-Hee,Lee Dae-Yeol 대한소아신장학회 2003 Childhood kidney diseases Vol.7 No.1

목적 : 본 연구에서는 신반흔의 고위험 환아를 규명하고자 요로감염 후 DMSA 신주사를 이용하여 급성기와 치료 후 추적 검사를 통하여 신반흔을 형성한 군과 신반흔을 형성하지 않은 군에서 방광요관 역류 유무와 등급, DMSA 신주사 소견 등을 비교 분석하여 신반흔에 관련된 인자들을 평가하고 신반흔의 예측인자로서 방광요관 역류와 급성기 DMSA 신주사 소견의 유용성을 알아보고자 하였다. 방법 : 1992년 4월부터 2002년 3월까지 전북 대학교 병원 소아과에 요로감염으로 입원한 환아 442명을 대상으로 후향적으로 조사하였다. 요로감염의 급성기에 급성기반응 지표, DMSA 신주사를 시행하였고 치료 후 VCUG를 시행하여 방광요관 역류를 진단하였고 치료 4-6개월 후 DMSA 신주사를 시행하여 신반흔 유무에 따라 두 군으로 나누어 비교 분석하였다. 결과 : 연령 분포는 두 군 모두 1세 미만이 가장 많았으나 신반흔 형성군에서는 진단당시 연령이 신반흔 무형성군에 비해 높았고 성별에 따른 유의한 차이는 보이지 않았다. 원인균으로는 E.coli가 가장 많았으며 두 군간에 원인균에 따른 차이는 없었고 급성기에 시행한 백혈구, ESR, CRP 등의 급성기 반응지표는 신반흔 형성과 상관관계를 보이지 않았다. 신반흔 형성군에서는 방광요관 역류의 빈도가 78%, 신반흔 무형성군에서는 13%이었고, grade IV 이상의 중증의 역류가 차지하는 비율이 신반흔 형성군에서 높았으며 방광요관역류와 그 정도가 신반흔 형성과 밀접한 관계가 있음을 보여주었다. 급성기에 시행한 DMSA 신주사 소견상 다초점성 신피질 결손의 빈도가 신반흔 형성군에서 53%로 신반흔 무형성군의 32%에 비해 유의하게 높았고 방광요관 역류가 있으면서 신주사상 다초점성 결손을 보인 환아의 75%에서 신반흔이 형성되었다. 결론 : 신반흔 형성은 방광요관 역류의 유무와 정도, 급성기의 DMSA 신주사 소견과 밀접한 관계가 있었다. 따라서 초기 신주사 소견과 방광요관 역류는 신반흔 발생의 위험을 예측하는데 도움을 주며 고위험군으로서 추적관찰에 유용한 정보를 제공해 줄 것으로 생각된다. Purpose : Acute pyelonephritis in children may result in permanant renal damage which later in life may lead to hypertension and renal failure. The purpose of this study was to evaluate the factors that might be useful for predicting the development of renal scar in children with urinary tract infection(UTI). Methods : We retrospectively reviewed 442 patients with UTI who were admitted to the Department of Pediatrics of Chonbuk National University Hospital, during the period from April 1992 to March 2002. The patients were divided into two groups according to the presence of renal scar on the follow-up DMSA renal scan, and we compared the factors associated with renal scarring between the two groups. Results : There were no significant differences in sex, causative organism and acute phase reactants between the groups with and without renal scar. The age at diagnosis was significantly higher in the renal scar group compared to that without scar. Of the 60 patients with renal scar, 78% had vesicoureteral reflux(VUR), but 13% of patients without scar had VUR. Furthermore, the severity of VUR was significantly correlated with renal scar formation. 53% showed multiple cortical defects on the initial DMSA renal scan, compared to 32% in the non-scar group. In addition, 76% of patients showing multiple cortical defects on the initial DMSA renal scan with VUR had renal scar. Conclusion : The presence and grade of VUR, and findings on the initial DMSA renal scan would contribute to predict risk of renal scar formation in children with UTI.

양육의 개념 분석

이수연,Lee Soo Yeon 한국아동간호학회 1998 Child Health Nursing Research Vol.4 No.1

Rearing is important to the growth and development of the child. Traditionally, nurturing is a maternal role which is expressive role. Maternal role was composed of mothering and maternicity. Rearing is a key concept of psychology, education and sociology discipline. Knowledge of the rearing is an essential component for the development of nursing as a science and a profession. The first thing to study a concept is thought to be a concept analysis. So, in this study, concept analysis of the rearing was performed to clarify a concept of the rearing as a basis for the study of rearing afterward. The approach used for the concept analysis was the approach presented by Walker and Avant (1993). The defining attributes of rearing, identified in this study were (1) a series of caring activities in parent-child relation, (2) an essential of the growth and development of a child, (3) changeable according to time, place and object, (4) effort is necessary to the rearing, (5) positive or negative aspects (6) attitude, behavior, environment is sub-concepts of the rearing concept. The identified antecedents of the rearing was child-birth. The identified consequences of rearing were desirable or undesirable outcomes. Desirable outcome was the emotional satisfaction and growth in parent-child relation. Undesirable outcome was the emotional disorder and social maladaptation of the child. The empirical referents of the rearing could be the caring phenomena in parent-child relation.

Antiestrogenic Effects of Marijuana Smoke Condensate and Cannabinoid Compounds

이수연,오승민,Sang Ki Lee,Kyu Hyuck Chung 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.12

The antiestrogenic effects of marijuana smoke condensate (MSC) and three major cannabinoids, i.e., ∆9-tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabinol (CBN), were evaluated using in vitro bioassays, viz., the human breast cancer cell proliferation assay, the recombinant human estrogen receptor (ER) competitive binding assay, and the reporter gene assay. The inhibitory effects on estrogen were also examined using the ethoxyresorufin-Odeethylase (EROD) assay, the aromatase assay, and the 17β-estradiol (E2) metabolism assay. The results showed that MSC induced the antiestrogenic effect via the ER-mediated pathway, while THC, CBD, and CBN did not have any antiestrogenic activity. This suggests that the combined effects of the marijuana smoke components are responsible for the antiestrogenicity of marijuana use. In addition, MSC induced the CYP1A activity and the E2 metabolism, but inhibited the aromatase activity, suggesting that the antiestrogenic activity of MSC is also related to the indirect ER-dependent pathway, as a result of the depletion of the in situ E2 level available to bind to the ER. In conclusion, pyrogenic products including polycyclic aromatic hydrocarbons (PAHs) in the non-polar fraction, which is the most biologically active fraction among the seven fractions of MSC, might be responsible for the antiestrogenic effect.

대사 산물 분석을 이용한 유전성 대사 질환 진단

이수연,Lee, Su-Yeon 대한유전성대사질환학회 2004 대한유전성대사질환학회지 Vol.4 No.1

Calculations of the Low-Lying Structures in the Even-Even Nd/Sm/Gd/Dy Isotopes

이수연,J. H. Lee,이영준 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.72 No.10

The nuclear structure of deformed nuclei has been studied using the interacting boson model (IBM). In this study, energy levels and E2 transition probabilities were determined for even nuclei in the Nd/Sm/Gd/Dy chains which have a transition characteristic between the rotational, SU(3) and vibrational, U(5) limits. The structure of the nuclei exhibits a slight breaking of the SU(3) symmetry in the direction of U(5), and therefore, we add the d-boson number operator nd, which is the main term of the U(5) symmetric Hamiltonian, to the SU(3) Hamiltonian of the IBM. The calculated results for low-lying energy levels and E2 transition rates in Nd/Sm/Gd/Dy isotopes are in reasonably good agreement with known experimental results.

압전에너지 하베스팅용 나노소재 연구 동향

이수연,Lee, Su-Yeon 한국전기전자재료학회 2018 전기전자재료 Vol.31 No.4

알레르기성 자반증에 병발한 대뇌 혈관염 1례

이수연,정주미,황동진,은소희,황평한,Lee, Soo Yeon,Choung, Ju Mi,Hwang, Dong Jin,Eun, So Hee,Hwang, Pyoung Han 대한소아청소년과학회 2002 Clinical and Experimental Pediatrics (CEP) Vol.45 No.12

본 저자들은 경련과 의식저하를 보인 알레르기성 자반증 환아에서 신경학적 합병증으로 대뇌 동맥의 혈관염이 발생된 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Henoch-Sh${\ddot{o}}$nlein Purpura(HSP) is an immuologically mediated systemic vasculitis of small blood vessels affecting predominantly the skin, gastrointestinal tract, joints, and kidneys. Clinical neurological manifestations such as headaches, behavioral changes, mental changes, seizures, and visual loss are described, but neurological complication are rare during the course of HSP. We experienced a case of an 8 year-old male with HSP who presented with seizures. Magnetic resonance imaging(MRI) showed multiple high signal intensity in both cortical and subcortical areas of frontal and parieto-occipital lobes and magnetic resonance(MR) angiogram showed stenosis of cerebral arteries, compatible with MRI and MR angiogram findings of cerebral vasculitis. We report this case with related literature.