Helicobacter pylori cagE 유전자 다형성이 위상피세포주에서 Interleukin-8 유도능에 미치는 영향

이수민,이학성,이인석,조유경,한혜원,오정환,박재명,최명규,정인식,Lee Su-Min,Lee Hak-Sung,Lee In-Suk,Co Yu-Kyung,Han Hae-Won,Oh Jung-Hwan,Park Jae-Myung,Choi Myung-Gyu,Chung In-Sik 한국생명과학회 2004 생명과학회지 Vol.14 No.6

Helicobacter pylori infection is highly prevalent, as high as 2/3 of whole population infected, in Korea. H. pylori infection initiates inflammation by induction of interleukin-8 through type IV secretion of CagA. It was recently suggested that induction failure of IL-8 is not associated with defect in cag PAI but associated with cagE locus diversity. This study was designed to investigate ability of 11-8 in-duction according to sequence variation within the cagE gene, cagA TP motifs and vacA m-types in vitro study using AGS cell-line, and to evaluate its association with different clinical outcome. Seventy-four H. pylori stains were isolated from 23 patients with gastric cancer (Ca), 24 subjects with gastritis (G) and 27 patients with duodenal ulcer (Du) in Kangnam St. Mary's Hospital, Seoul, Korea. cagE gene diversity was confirmed by the PCR-RFLP methods with MboI/NlaIII and tyrosine phosphate motifs (TPMs) of cagA was determined TPM-A and C by using DdeI/Tsp5091 restriction enzyme and TPM-B was determend by Real time PCR the method of Owen et al. and IL-8 was measured by ELISA assay. IL-8 activity was positively detected in 59 among 74 strains $(79.7\%)$. IL-8 secretion was significantly increased in MboI A and MboI B type compared to MboI C type and in MboI/NlaIII A-C and B-C type than C-C type. 1L-8 activity was not associated with either the number or composition of cagA tyrosine phosphorylation motifs and vacA m-type. There was no significant difference in IL-8 activity among patient groups. cagE gene diversity is thought to be mainly associated with the induction of IL-8 in H. pylori infection. 한국에서 분리한 Helicobacter pylori 균주가 위암 세포주에서 IL-8를 유도하는 유도능을 관찰하고자 하였으며, 특히 4형 분비구조의 주요 구성체인 cagE 유전자의 다형성에 따라 IL-8 유도능의 차이 및 각 질환에서 분리한 균주에서의 차이가 있는지 검토하고자 하였다. 강남성모병원 내시경검사를 받은 환자들 중 H. pylori 감염 양성으로 확인된 위암 23명 $(31.0\%)$, 위염 24명$(32.6\%)$, 십이지장궤양27명$(36.4\%)$ 총 74명을 대상으로 하여 H. pylori를 분리한 후 PCR-RFLP방법을 이용하여 cagE 다형성을 확인하였으며, PCR 및 Real-time PCR을 이용하여 ragA TPMs를 결정하였다, 또한 분리된 균주를 위암세포주에 감염시켜 상등액을 취하여 ELISA방법으로 IL-8을 정량화 하였다. 전체 74개의 분리 균주 중 59개의 균주$(79.7\%)$에서 IL-8 유도능이 있는 것으로 확인되었으며, cagE 다형성 중에서 가장 높은 빈도로 관찰되는 형태는 MboI/NlaIII B-C, A-C 및 C-C 타입 이었으며, 이중 A-C와 B-C 형태가 C-C 형태에 비해 유의적으로 높은 IL-8 유도능을 나타냈다. 그러나 cagA TPMs의 분류 및 vacA m-아형 분류와 IL-8 유도능과는 연관성이 없었다. 비록 본 연구에서 cagE 다형성과 IL-8 유도능과 아주 밀접한 연관성이 나타나지는 않았지만 vacA m-아형이나 cagA TPMs 보다는 cagE 다형성이 IL-8 유도능과 연관성이 클 것으로 생각된다.

전이된 담도암 환자의 패혈증을 한다열소탕 및 우담과 항생제를 병행하여 치료한 환자 1례

이수민,최성헌,송안나,이지영,채진,정의홍,이수경,Lee, Soo-Min,Choi, Seong-Heon,Song, An-Na,Lee, Ji-Young,Chae, Jin,Jung, Eu-Hong,Lee, Soo-Kyung 사상체질의학회 2013 사상체질의학회지 Vol.25 No.4

Objectives Despite the treatment with antibiotics, patients with sepsis has a high mortality (80%) in the underlying disease group. The aim of this study was to report the improvement of septic condition of the cholangiocarcinoma patient after the treatment with Handayeolso-tang, Fel Tauri, and antibiotics. Methods We retrospectively reviewed the medical records. The patient's subjective symptoms such as chilling and abdominal pain were evaluated by NRS and the performance status was evaluated by ECOG. This case was literally compared with relevant published studies on prognosis of sepsis. Results Despite poor prognostic factor(MEDS score 18), the patient's symptoms such as fever, chilling, abdominal pain, and diarrhea and ECOG(Eastern Cooperative Oncology Group) improved. The patient was hemodynamically stabilized on 3rd day from the treatment, and her laboratory test results were normalized on 7th day. Conclusions A female patient of metastatic cholangiocarcinoma came to the hospital for cholangitis, later causing septic shock. Both her symptoms and laboratory tests showed significant improvement after the treatment of antibiotics, Handayeolso-tang and Fel Tauri. To our knowledge, this is the first case reporting the synergistic combination of Korean oriental medicine and Western medicine approaching to sepsis.

죽엽석고탕으로 방사선 치료 후 발생한 야간해수, 구갈, 피로가 개선된 유방암 환자 1례

이수민,이지영,채진,최성헌,송안나,이수경,정의홍,Lee, Soomin,Lee, Jeeyoung,Chae, Jin,Choi, Seongheon,Song, Anna,Lee, Sookyung,Jung, Yeehong 대한암한의학회 2013 大韓癌韓醫學會誌 Vol.18 No.1

Objectives : The purpose of this case study is to demonstrate the effectiveness of Traditional Korean Medicine as an adjunctive management for breast cancer treatment. Methods : A 56 year-old female patient diagnosed with stage IA(pT1cN0M0) of breast cancer was admitted our hospital. The patient had surgical resection followed by radiation therapy, and conducted adjuvant therapy with aromatase inhibitor. The patient's chief complaints were dry cough, thirst and fatigue, all of which were caused by radiation therapy. Traditional Korean herbal medicine was prescribed for symptom management. The patient's subjective symptoms such as cough, thirst and fatigue were evaluated by NRS. Results : Fatigue was significantly improved within a week. The severity of dry cough and thirst also was decreased after a few courses of herbal medicine treatment. Conclusion : This case study suggests that Tradition Korean Medicine would be beneficial for the patients who are suffering from adverse effects of the standard treatment of cancer.

Cervical Spine CT Using Spectral Shaping: Can It Be a Solution to Overcome Artifacts in the Lower Cervical Spinal Region?

이수민,추혜정,이선주,김성관,In Sook Lee,김동욱,Jin Wook Baek,허영진 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.3

Objective: To investigate the image quality, radiation dose, and intermodality agreement of cervical spine CT using spectral shaping at 140 kVp by a tin filter (Sn140-kVp) in comparison with those of conventional CT at 120 kVp. Materials and Methods: Patients who had undergone cervical spine CT with Sn140-kVp (n = 58) and conventional 120 kVp (n = 49) were included. Qualitative image quality was analyzed using a 5-point Likert scale. Quantitative image quality was assessed by measuring the noise and attenuation within the central spinal canals at C3/4, C6/7, and C7/T1 levels. Radiation doses received by patients were estimated. The intermodality agreement for disc morphology between CT and MRI was assessed at C3/4, C5/6, C6/7, and C7/T1 levels in 75 patients who had undergone cervical spine MRI as well as CT. Results: Qualitative image quality was significantly superior in Sn140-kVp scans than in the conventional scans (p < 0.001). At C7/T1 level, the noise was significantly lower and the decrease in attenuation was significantly less in Sn140-kVp scans, than in the conventional scans (p < 0.001). Radiation doses were significantly reduced in Sn140-kVp scans by 50% (effective dose: 1.0 ± 0.1 mSv vs. 2.0 ± 0.4 mSv; p < 0.001). Intermodality agreement in the lower cervical spine region tended to be better in Sn140-kVp acquisitions than in the conventional acquisitions. Conclusion: Cervical spine CT using Sn140-kVp improves image quality of the lower cervical region without increasing the radiation dose. Thus, this protocol can be helpful to overcome the artifacts in the lower cervical spine CT images.

수도 서울에서 정부종합청사의 입지와 건립

이수민,우동선,Lee, Sumin,Woo, Don-Son 한국건축역사학회 2018 건축역사연구 Vol.27 No.2

This study examines the site and erection of the Government Complex Seoul which was a project attempted to assemble dispersed government buildings in a certain place. The study focuses on the fact that the project is situated between the 1960s' making of capital Seoul and Seoul urban planning, and the way how the project achieved symbolism in capital Seoul. The project, one of the 1960s' Major Government Buildings, led both plan of capital Seoul and transforming city Seoul. The 1960s' Major Government Building Plan had identical drive with the 1950's Major Government Building Plan, however the 1960s' had additional layer: Seoul urban planning. After restoration of the Capital building, Sejongro the capital street was planned to the site arranging government offices. The Government Complex Seoul was set to be a modern building on a site with historical context according to the plan. Because of the site, the Government Complex Seoul was constructed in aware of other buildings that represented a competitive high-rise atmosphere in the late 1960s, including the Capital building nearby. PAE International's plan was completed through a series of design modification, and it boasted a vertical aspect, unlike the horizontal-looking plan that was already won after the design competition. The Government Complex Seoul tried to acquire the symbolism in the central space of the capital Seoul and high-rised city Seoul. "The new construction method" was a requirement to achieve the height.

비살균원유치즈의 산업 현황

이수민,이희영,윤요한,Lee, Soomin,Lee, Heeyoung,Yoon, Yohan 한국축산식품학회 2015 축산식품과학과 산업 Vol.4 No.1

긴급한 미생물학적 위해평가 시 데이터 갭 보완 방안에 대한 의견

이수민,윤요한,Lee, Soomin,Yoon, Yohan 한국축산식품학회 2016 축산식품과학과 산업 Vol.5 No.1

분양임대혼합아파트 단지의 소셜믹스에 대한 관리자 및 거주자 의식조사

이수민,김영주,Lee, Soomin,Kim, Youngjoo 한국주거학회 2014 한국주거학회 논문집 Vol.25 No.6

The purpose of this study was to identify the perception on social mix of managers and residents in the mixed housing complexes for sale and rental apartment. For the research purpose, concept and background of social mix, relevant policies, preceding research trend of social mix were organized through the literature analysis. In addition, observation study was executed to examine physical aspects of three selected sites as research subjects. Also, current state and awareness for social mix were conducted by using in-depth interview with 6 managers and 30 residents in selected areas with semi-structured questionnaires from June 3 to 7, 2013. The results of the study are as following: In the aspect of management, managers said that the biggest issue of mix-housing complex was dualization of management subject such as delegate of residents and tenants. Therefore, legislation which is suitable for unique features of mix-housing complex is required. To decrease disharmony between condominium and rental apartment, controlling the distribution ratio between condominium and rental apartment can be a good way. Accommodating middle class in mixed-housing complex is considered to reduce the social gap. Socially, multilateral and continuous effort through government, industry and academic area is required to inspire positive awareness on social mix among residents in mixed housing complex.

사상인 체질병증 험위증(險危證) 중 악성 종양으로 유발될 수 있는 증(證)에 대한 고찰

이수민,박소라,이수경,Lee, Soo-Min,Park, So-Ra,Lee, Soo-Kyung 사상체질의학회 2015 사상체질의학회지 Vol.27 No.3

Objectives The purpose of study was to identify cancer related symptoms of Sasang Constitution based on the classic of Sasang Constitutional Medicine (SCM). Methods The bibliographical study was performed with "Dongyisoosebowon-Shinchukbon(東醫壽世保元 辛丑本)", Dongyisoosebowon-Sasangchobongwon(東醫壽世保元 四象草本券), "Cancer", and several review articles. The perspective on severe illness in SCM was investigated. And 'the critical state' of constitutional symptoms based on "Dongyisoosebowon" was identified as cancer related symptoms. Results and Conclusions The perspective on severe illness of SCM was focused on the human being itself, compared with symptom based traditional Chinese medicine. The preservation of requisite energy (保命之主) was a vital factor of longevity to maintain healthy status and the classification of severity of disease. And critical state was an important indicator to control severe illness. Regarding cancer related symptoms in SCM, Janggwol(臟厥), Eumsung-gyeokyang(陰盛隔陽證), Janggyeol(藏結證) of Soeumin symptoms, Hwangdal(黃疸), Haso(下消), Eumheo-oyel(陰虛午熱證), Gochang, Tohyul(吐血) of Soyangin's symptoms, Joyeol(燥熱證), Bokchang-bujong(腹脹浮腫) of Taeeumin's symptoms, and Eolgyek of Taeyangin's symptoms could be shown the association with cancer. According to the prognosis on disease severity, regimens of daily life, behavior modifications as well as medications were also emphasized with great importance to control severe illness in SCM. These holistic approach for controlling severe illness in SCM could lead to the improvement of treatment outcome.

구성주의 관점에서 스토리텔링을 활용한 초등 수학 수업 자료 분석

이수민,김진호,Lee, Sumin,Kim, Jinho 한국수학교육학회 2014 初等 數學敎育 Vol.17 No.3

New teaching and learning theory on various aspects about class is needed to implement education which reflects constructivism, ideally. For an ideal learner-centered mathematics class, tangible and intangible elements related to education(view of knowledge, view of leaner, teacher's role, evaluation, the form of class, learning, teaching material, etc.) should be integrated from a constructive perspective and especially, teaching material has to be premised on that learners have intellectual abilities to construct knowledge themselves, and reflect integrity of knowledge, diversity and others, and contain open attributes. In addition to this, teaching material should have characteristics different from those when objective epistemology applies, so there is a need to analyze whether teaching material has those characteristics. For this, this study compared and analyzed <1. Three-Digit Numbers> which belongs to the domain of numbers and operations out of the units of mathematics(3) textbook of the 2009 revised curriculum for the first and second grade that first introduced story-telling, and <3. Understanding of Place Values> for the second grade of constructive math class used in the U.S. 구성주의를 반영한 대표적인 교수 학습 이론인 학습자 중심 수학 수업이 이상적으로 실천되기 위해서는 구성주의 관점에 따른 교육과 관련된 유무형의 제 요소들(지식관, 학습자관, 교사의 역할, 평가, 수업 형식, 학습, 수업 자료 등)이 융합되어야 한다. 그 중 수업 자료는 학습자들이 스스로 지식을 구성할 수 있는 지적 능력이 있음을 전제로 개발되어야 하고, 지식의 통합성, 다양성 등이 반영되어야 할 것이며 개방형 속성을 지닌 자료이어야 한다. 이외에도 객관적 인식론을 적용할 때 갖던 특징과는 다른 특징들을 지녀야 하므로, 수업 자료가 그러한 특징들을 가지고 있는지 분석해 볼 필요가 있다. 이를 위해 2009 개정 교육과정 1-2 학년군 수학(3) 교과서의 스토리텔링이 처음 도입된 단원 중 수와 연산 영역에 해당되는 <1. 세 자리수>와 미국에서 사용되고 있는 구성주의 수학교실 2학년의 <3. 자릿값의 이해>를 비교하여 분석하였다.