Immunoblot 법을 이용한 간흡충항원(肝吸蟲抗原)의 발육단계별(發育段階別) 항원성분석(抗原性分析)에 관한 연구(硏究)

이선경,주경환,정명숙,임한종,Lee, Seon-Kyung,Joo, Kyoung-Hwan,Chung, Myung-Sook,Rim, Han-Jong 한국농촌의학지역보건학회 1991 농촌의학·지역보건 Vol.16 No.1

Serodiagnosis of Clonorchis sinensis infections will probably be a first choice tool for screening of clonorchiasis in a future because of increasing difficulties in collection and examination of stools. The sensitive test such as ELISA can he used effectively. However there are some limitations in serological diagnosis for the detection of serum antibody. One of the major problems is the non-specificity of the antigens which produce cross reaction with other helminthic infection sera. To solve this problem. many investigators have tried to purify the antigens used. In this study, we determined the antigenic profile of the crude saline extract antigen of C. sinensis at early developmental stage based on SDS-PAGE and immunoblotting techniques for the purpose of understanding the nature of C. sinensis worm antigen The following results were obtained : 1) The SDS-PAGE showed many protein hands ranging from 10Kd to 91Kd relative molecular weight. Among them, 66, 46, 40, 33, 27, 24, 16, 14 and 10Kd bands were observed as a principle bands. The protein components of C. sinensis changed chronologically during their early developmental period. 44Kd band was stained unclearly in antigen of 2 weeks worm, but changed to concentrated state in antigen of 5 weeks worm. 35Kd band was found in antigen of 2 weeks worm, however this band was disappeared in antigen of 5 weeks worm. 22Kd band also lost its staining property gradually. 2) In spite of differences in antigenic profile, there was no differences in the data obtained by microplate ELISA using each antigen preparation. Absorbance value began to rise in between 2 to 3 weeks after infection. 3) By EITB. serum antibody recognized major protein bands with molecular weight of 91, 85, 63, 46, 40, 33, 24, 14 and 10Kd hand respectively. Among them 66, 33, 17 and 14Kd bands were observed as non-specific band because they reacted even in normal control sera. Generally, gradual increase of positive reactions were observed as the infection period of C. sinensis was prolonged. In other hand, the reaction of 10Kd hand did not occurred when 26th week sera was tested. 4) The positive reactions using antigens of 2 weeks worm, especially on 40 and 24Kd bands, were most strong and sharply demarcated compared to those of 3~5 weeks worm antigen.

좌측 난관 임신 수술 후 진단된 겸한 임신 1례

이선경 ( Seon Kyoung Lee ),노현경 ( Hyon Kyung Rho ),강규현 ( Kue Hyun Kang ),지정석 ( Jung Suk Jee ),김상용 ( Sang Yong Kim ),조태일 ( Tae Il Joe ),윤귀옥 ( Gui Okh Yoon ) 대한주산의학회 2007 Perinatology Vol.18 No.3

Heterotopic pregnancy with coexistiong intrauterine and extrauterine pregnancy, is a rare event in natural pregnancy. However, in recent years, the increase in the incidence of pelvic inflammatory disease and use of assisted reproductive technology had led to an increase in the frequency of heterotopic pregnancy. When the diagnosis of heterotopic pregnancy is delayed, maternal mortality and morbidity may be increased, and unwanted loss of intrauterine pregnancy may occur. Therefore, the early diagnosis of heterotopic pregnancy is clinically important, and clinicians have to consider the possibility of heterotopic pregnancy and conduct careful and thorough evaluation if suspected. We experienced a case of heterotopic pregnancy in a woman in the absence of the known risk factors. She underwent underwent spontaneous abortion of intrauterine pregnancy at postoperative 4th day, after laparoscopic salpingectomy for left tubal pregnancy. We report this case with brief review of literature.

DBP 스폰지와 DBP/PLGA 지지체에서의 인간 디스크세포 거동분석 비교

이선경(Seon Kyoung Lee),홍희경(Hee Kyung Hong),김수진(Su Jin Kim),김용기(Yong Ki Kim),송이슬(Yi Seul Song),하윤(Yoon Ha),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2010 폴리머 Vol.34 No.5

본 연구팀은 DBP를 함침시킨 물성이 서로 다른 스폰지와 PLGA 지지체를 제작한 후 세포 부착, 증식 및 형태 유지를 알아보기 위한 실험을 수행하였다. WST 분석법과 SEM 관찰을 통하여 스폰지에 비해서 PLGA 지지체에서의 세포의 증식이 활발한 것을 확인하였고, RT-PCR을 통해 디스크세포에서 특이적으로 발현하는 제2형 콜라겐과 어그리칸의 발현을 확인하였다. WST 결과, 세포 증식률은 DBP/PLGA 지지체가 DBP를 함침시킨 스폰지보다 세포 증식률이 높음을 확인하였다. 본 연구팀은 스폰지보다 PLGA 지지체가 인간디스크의 표현형 유지 및 중식에 있어서 긍정적인 영향을 미치는 것을 확인하였다. We fabricated sponge and poly(lactide-co-glycolide) (PLGA) scaffolds impregnated demi-neralized bone particle(DBP) (DBP/PLGA) and investigated proper condition to proliferation and phenotype maintenance of intervertebral disc(IVD) cells by comparison between DBP/PLGA scaffold and DBP sponge. DBP/PIGGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in scaffolds of two types. Cell viability and proliferation according to scaffolds were analyzed by WST assay and SEM. RT-PCR was assessed to measure mRNA expression of aggrecan and type Ⅱ collagen of human IVD cells. In WST assay results, cell viability in scaffolds impregnated DBP/PLGA scaffold were higher than DBP sponge. We could observe that disc cell mRNA expressed better in DBP/PLGA scaffold than DBP sponge. We concluded that the using of DBP/PLGA in terms of scaffold fabrication for bio-disc with human IVD cells is helpful growth of disc cells maintenance of phenotypes.

자궁경부 상피내 종양과 침윤성 편평상피암종에서 claudin-1, claudin-4, zonula occludens-1의 발현

이선경 ( Seon Kyoung Lee ),노현경 ( Hyun Kyung Rho ),박태양 ( Tai Yang Park ),강규현 ( Kue Hyun Kang ),조태일 ( Tae Il Cho ),이태진 ( Tae Jin Lee ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.10

케라틴/PLGA 복합체 필름의 적층 원통형 지지체가 섬유륜 재생에 미치는 영향

이선경(Seon Kyoung Lee),홍희경(Hee Kyung Hong),김수진(Su Jin Kim),김용기(Yong Ki Kim),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2010 폴리머 Vol.34 No.5

조직공학에 이용되는 고분자인 poly(lactic-co-glycolic acid) (PLGA)는 높은 생체적합성 및 생분해성의 장점을 지니지만, 생체활성물질의 결여와 소수성으로 인해 세포의 부착에 어려움을 가진다. 또한 PLGA의 가수분해 과정 중 생성되는 산 분해물이 조직주변의 pH를 감소시켜 염증을 유발하는 단점을 가지고 있다. 따라서 이러한 단점을 보완하기 위한 방법으로 천연고분자인 케라틴을 첨가한 PLGA필름을 제작하였으며 조직공학적 디스크재생에 응용하기 위하여 케라틴/PLGA 복합체필름의 원통형 지지체를 제작하였다. 이 원통형 지지체의 내부는 케라틴/PLGA 필름을 말아서 사용하였으며 외부는 PLGA 다공성 지지체 링을 제작하여 사용하였다. 본 연구에서는 이 지지체에 섬유륜세포를 파종하여 PLGA 필름과 케라틴/PLGA 필름을 이용한 원통형 지지체에서의 세포의 부착 및 생존율을 비교하고 케라틴을 첨가한 지지체의 우수성을 확인하였으며 강도의 우수성 또한 확언하였다. 이 결과는 디스크재생을 위한 지지체의 연구에 유용한 정보를 제공할 것이다. We developed laminated cylindrical scaffolds composed of poly(lactide-co-glycolide) (PLGA) and keratin, and investigated their potential for tissue engineering and disk regeneration. The scaffold was designed to have two parts, i.e. inner cylinder and outer disk, to mimic a natural disk. The outer disk was composed of PLGA and the inner cylinder was prepared using PLGA film or PLGA/keratin hybrid film. In this study, we investigated the effects of keratin on the growth and proliferation of annulus fibrous(AF) cells in the cylindrical scaffolds. Scaffolds containing PLGA/keratin films showed a significantly higher cell proliferation and expression of collagen I and II than the counterpart with PLGA films. Keratin containing scaffolds also exhibited an excellent mechanical strength, demonstrating that keratin influences the proliferation of annulus fibrous cells. The results provide valuable information on PLGA/keratin films for tissue engineered disk regeneration.

한국어 말소리 재인 시 표기의 영향

이선경 ( Seon Kyoung Lee ),이윤형 ( Yoon Hyoung Lee ),권유안 ( You An Kwon ) 경남대학교 인문과학연구소 2016 人文論叢 Vol.41 No.-

본 연구는 말소리 재인 시 표기 정보가 사용되는지 아닌지를 검증하기 위해 수행되었다. 양방향 상호 활성화 모형을 바탕으로 과거 많은 연구들이 주로 시각적 단어재인에서 음운의 역할을 다룬 반면 말소리 재인에서 표기의 역할을 검토한 연구는 상대적으로 적었다. 이를 위해 본 연구에서는 교차-양상 청각 단어 어휘 판단과제를 통해 한국어 말소리 재인에서 표기와 음운의 영향을 살펴보고자 하였다. 본 연구에서 점화자극은 시각적으로 제시되었고, 목표자극은 청각적으로 제시되었다. 그 결과 점화자극과 목표자극의 첫 음절 표기가 일치하는 조건에서 유의하게 촉진적인 점화효과가 관찰되었고, 첫 음절 음운이 일치하는 조건에서는 억제적인 점화효과가 관찰되었다. 이에 따라 본 연구는 말소리를 재인할 때도 표기 정보가 활용될 수 있음을 제시하였다. The purpose of this study is to examine the role of orthographic information and phonological information in spoken word recognition in Korean. While many studies suggested the effect of the phonological information in visual word recognition in Korean, less is known about the effect of the orthographic information in spoken word recognition in Korean. In this study, participants performed cross modal masked priming lexical decision tasks where a prime was visually presented before auditory target were presented. The results showed that the orthographically matched prime facilitated lexical decision of the auditory targets while the phonologically matched prime caused inhibition. These results support a bimodal interactive activation model by Grainger and colleagues in the 1990s, which suggests the interaction of the orthographic and phonological information in sublexical and whole-word representations.

유아교사의 교수몰입도에 따른 유아의 놀이몰입 및 자기효능감

이선경(Lee seon kyung),심성경(Sung-Kyoung Sim) 한국육아지원학회 2016 육아지원연구 Vol.11 No.3

본 연구는 유아교사의 교수몰입 경험에 대한 전반적인 양상과 변인별 차이를 탐색하고, 유아교사의 교 수몰입도 수준에 따른 유아의 놀이몰입과 자기효능감의 차이를 밝히기 위해 수행되었다. 유아교사의 교 수몰입도를 알아보기 위해 전라북도 J, I, K시에 소재한 유아교육기관의 만 5세 담당 교사 227명을 대상 으로 하였다. 교수몰입도에 따른 유아의 놀이몰입, 유아의 자기효능감을 분석하기 위해 교수몰입도 분포 중 상집단 10%(23명)와 하집단 10%(23명)중에서 학력, 경력 등의 동질성을 고려하여 상집단의 교사 3명 과 유아 63명, 하집단의 교사 3명과 유아 59명을 표집하였다. 본 연구에서 얻어진 결과는 다음과 같다. 첫 째, 유아교사의 교수몰입도는 중간보다 약간 높은 수준이며, 연령과 경력이 많을수록, 기혼일 때 더 높았 으며, 월 급여가 가장 높은 교사가 몰입도 수준이 가장 높았다. 둘째, 교수몰입도 수준이 높은 교사의 유 아가 낮은 교사의 유아에 비해 놀이몰입 수준이 더 높았다. 셋째, 교수몰입도 수준이 높은 교사의 유아가 낮은 교사의 유아에 비해 자기효능감은 전체적으로 차이가 없었으나 부분적으로 인지적 영역의 자기효능감이 높았다. The purpose of this study was to investigate the general status of early childhood teachers' teaching flow and the differences by individual and environmental variable and then to analyze young children's play flow and self-efficacy in relation to early childhood teacher's teaching flow level. The subjects of this study were 227 teachers in charge of five-year-old children at early childhood educational institutions located in J, I and K cities in Cheollabuk province. For the purpose of analyzing young children's play flow and self-efficacy in relation to the level of teaching flow, this study sampled total 6 teachers out of the teaching flow distribution of the above 227 teachers considering the similarity between teachers' academic career, work career, etc, and also 122 young children in their charge. They consist of 3 teachers out of the highest 10% group (23 teachers) of the distribution and all of the 63 young children in their charge, and 3 teachers out of the lowest 10% group (23 teachers) and all of the 59 young children in their charge, respectively. The results of this study are as follows. First, early childhood teachers' teaching flow was slightly above the middle. The older and the more experienced the teachers were, the higher the teaching flow was. Also, it was higher among the married teachers group. Besides, the teachers getting the highest monthly wage showed the highest teaching flow. Second, young children in the class of teachers with higher teaching flow showed higher level of play flow. Third, though children in the class of teachers with higher teaching flow showed no significant difference in self-efficacy compared to children in the class of teachers with lower teaching flow in general, they showed higher self-efficacy in the sub-factor, cognition, in part.

Immunoblot 법을 이용한 肝吸蟲抗原의 發育段階別 抗原性分析에 관한 硏究

李宣璟(Seon-Kyung Lee),朱炅煥(Kyoung-Hwan Joo),鄭明淑(Myung-Sook),林漢鐘(Han-Jong Rim) 한국농촌의학 지역보건학회 1991 농촌의학·지역보건 Vol.16 No.1

의료보장 형태가 혈액투석 서비스 제공에 미치는 영향에 대한 다수준 분석

정진희,권순만,김경훈,이선경,김동숙,Jung, Jin-Hee,Kwon, Soon-Man,Kim, Kyoung-Hoon,Lee, Seon-Kyoung,Kim, Dong-Sook 대한예방의학회 2010 예방의학회지 Vol.43 No.3

Objectives: This study aims to compare quality indicators for the hemodialysis services between patients with health insurance and those with medical aid. Methods: This study used data from sampled hospitals that provided a hemodialysis service. A total of 2287 patients were selected, and the information for hemodialysis service has been granted from medical record reviews. A multi-level regression analysis was used to examine the differences in process and outcome indicators for hemodialysis between patients with health insurance and those with medical aid. Process indicators were defined as: frequency of hemodialysis, hemodialysis time, erythropoietin (EPO) use, measurement of hemodialysis dose at least once a month, measurement of phosphate at least once every three months, and measurement of albumin at least once every three months. Outcome indicators were defined as: hemodialysis adequacy, anemia management, blood pressure management, and calcium, phosphate and nutrition management. The total scores for outcome indicators ranged from 0 (worst) to 4 (best). Results: There was a significant difference in the measurement of hemodialysis dose at least once a month between patients with health insurance and those with medical aid (OR 0.66, 95% CI = 0.43 - 0.99). However, frequency of hemodialysis, hemodialysis time, EPO use, measurement of phosphate at least once every three months, measurement of albumin at least once every three months, hemodialysis adequacy management, Hb${\geq}$11 g/dL, blood pressure within the range of 100-140 /60-90 mmHg, calcium x phosphate${\leq}$55 $g^2/dL^2$ and albumin${\geq}$4 g/dL were not significantly different between the groups. Conclusions: There were no significant differences in outcome indicators for hemodialysis between the groups. Further studies are warranted into the mechanism that results in no differences in the outcome indicators for hemodialysis.

히알루론산이 함유된 SIS 스폰지와 섬유륜세포를 이용한 디스크재생

홍희경(Hee Kyung Hong),이선경(Seon Kyoung Lee),송이슬(Yi Seul Song),김대성(Dae Sung Kim),엄신(Shin Eom),김형은(Hyoung Eun Kim),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2010 폴리머 Vol.34 No.3