High Pressure Freezing (HPF)을 이용한 조류 Ptilota filicina의 미세구조 관찰:HPF 고정법과 화학 고정법의 비교

이상희,김윤중,정종만,김진규,김영민,권희석,문원진,이석훈,Lee, Sang-Hee,Kim, Youn-Joong,Jeong, Jong-Man,Kim, Jin-Gyu,Kim, Young-Min,Kweon, Hee-Seok,Moon, Won-Jin,Lee, Seok-Hoon 한국조류학회(藻類) 2006 ALGAE Vol.21 No.4

In preparation of the biological samples for electron microscopy, the chemical fixation by glutaraldehyde, paraformaldehyde, and OsO4 has been generally used for a long time. However, the chemical fixation method has some problems: the infiltration time is a little bit long and the ultrastructure of cell or tissue transforms before complete fixation of sample. So, recently, cryo-fixation is considered more often in biomedical field. In this study, we compared High Pressure Freezing (HPF) method with chemical fixation method using a algal sample (Ptilota filicina J. Agardh), which was difficult to fix using chemical fixation method. In chloroplast, the ultrastructure of thylakoid lamella and phycobilisome can not show clearly by chemical fixation. In this study we could observe the ultrastructure of thylakoid lamella and phycobilisome of chloroplast very clearly using HPF fixation. An improved images of ultrastructures of nucleus, mitochondrion and floridean starch could obtain. These results suggest that HPF method is very useful method in algal specimen for electron microscopy.

그물형 하천에서의 용존산소 시뮬레이션

이상희,박석순 ( Sang Hee Lee,Seok Soon Park ) 한국물환경학회 1997 한국물환경학회지 Vol.13 No.1

A water quality modeling study was performed for network stream located at western part of Lower Nakdong River. Model construction was based on SNSIM(Stream Network Simulation Model which was developed by United States Environmental Protection Agency. The study area includes Western Nakdong River, Joman River, and their tributaries. The area was divided into 22 reaches according to the model structure. Each reach was then subdivided into uniform computational element of 0.1km. In order to characterize study area, two synoptic field surveys were performed during February 6∼7 and March 29∼30, 1991 periods at network stream and input tributaries. The model was calibrated to the February survey data and verified to the March survey data. The water quality parameters simulated in the model included dissolved oxygen(DO), carbonaceous biochemical oxygen demand(CBOD), and nitrogenous biochemical oxygen demand (NBOD). Computer code of SNSIM model was modified in this study to be executable on IBM PC or comparable with metric input/output unit. The study indicated that the modified SNSIM model would be an effective tool to simulate the water qualities in the complicated stream network. The predicted water qualities displayed reasonable agreements with the field measurements during both surveys.

남 · 북한강 유입 지천 수질 비교를 위한 윌콕슨 순위합 검정법 적용

이상희,박석순 ( Sang Hee Lee,Seok Soon Park ) 대한환경공학회 1993 환경종합 학술대회 Vol.- No.-

신기술 신소재 - 식품산업에서의 분자 각인 고분자 (Molecularly Imprinted Polymer)의 활용

이상희,Lee, Sang-Hee 한국식품연구원 2011 食品技術 Vol.24 No.4

타우린 처리에 의한 나리 기내 식물체 생산체계 최적화

이상희,양환래,김선태,전태환,김용철,김종보,Lee, Sang-Hee,Yang, Hwan-Rae,Kim, Sun Tae,Jun, Tae Hwan,Kim, Yong Chul,Kim, Jong Bo 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.4

나리는 절화용으로 대한민국에서 인기 있는 작물 중 하나로 고품질의 우수한 나리 품종이 개발되어야 한다. 이러한 우수 품종이 개발된 후에는 고효율의 번식체계가 확립되어야 한다. 본 연구는 타우린 처리에 의해서 신초 및 구근형성, 생체중 증가 및 발근율 향상 등의 생장촉진 효과와 갈변율 감소효과를 통한 나리 '레드플레임' 품종의 기내 식물체 생육 조건 체계 확립을 위해 수행되었다. 타우린이 생장촉진물질로 효능이 있는지 검증하기 위해 타우린을 0, 2.5, 5. 10, 15 및 20 mg/l을 첨가하였다. 그 결과, 타우린 20 mg/l 처리구에서 85 %의 신초형성률을 나타내었고 생체중은 5.5배 증가로 무처리구에서의 4.35배보다 더 높은 증가를 보였다. 구근형성 및 발근율 촉진 효과로는 타우린 20 mg/l에서 80%의 multiple 구근 형성률과 82%의 발근율을 나타내었다. 무처리구는 각각 50%의 multiple 구근 형성률과 60%의 발근율로 타우린을 첨가한 처리구의 효율이 더높았다. 또한 타우린 2.5 mg/l 처리구에서는 10.6%의 갈변율을 보인 반면에, 20 mg/l 처리구에서 0.8%의 갈변율을 보여 갈변율 감소에도 효과를 나타내었다. 타우린 처리구가 생체중 증가, 신초 생성률, 발근율 및 구근 형성 등의 전반적인 생장요인 들에 있어서 긍정적인 효과를 보여 줌으로써 나리를 포함하는 식물조직배양에서 타우린이 구연산 및 비타민 C와 같은 항산화제 또는 아미노산 대체제로서 사용 가능성을 보여 주었다. Lilies as cut flowers are one of the most popular ornamental plants in South Korea. It is necessary to develop lily cultivars with high qualities. Therefore, highly efficient propagation systems are needed following release of elite cultivars. In this study, we used taurine treatment to improve the growth conditions including shoot and bulb formation, fresh weight gain, and reduction of rooting and browning. We experimentally evaluated the effect of taurine as a growth stimulator, at concentrations of 0, 2.5, 5, 10, 15 and 20 mg/l. The results showed that 20 mg of taurine enhanced shoot formation by 85% and increased fresh weight 5.5-fold, which was higher than the approximately four-fold increase in the control. In addition, multiple bulb formation rate was increased by 80% and rooting by 82% following exposure to 20 mg/l of taurine. The efficiency of taurine treatment was higher than that of control with 50% multiple bulb formation rate and 60% rooting rate. The browning was 10.6% at 2.5 mg/l of taurine when compared with 0.8% at 20 mg/l. Taurine showed a positive effect on the overall growth of lily plants in terms of increased fresh weight, shoot formation rate, rooting, and formation of multiple bulbs, indicating that taurine can be used as an alternative to amino acids or as an antioxidant such as citrate and vitamin C in plant tissue culture.

중풍환자의 구(灸)치료에 따른 기능회복도에 관한 임상연구

이상희,김재규,손연희,정현윤,김정훈,권정남,김영균,Lee, Sang-Hee,Kim, Jae-Kyu,Son, Yeon-Hui,Jeong, Hyun-Yun,Kim, Jung-Hoon,Kwon, Jung-Nam,Kim, Young-Kyun 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.1

Objective : The purpose of this clinical study was to investigate the effects of moxibustion on functional recovery in stroke patients. Methods : Forty two stroke patients were randomized into either the standard physiotherapy treatment combined with moxibustion group or a control group with standard physiotherapy alone. They were 8 weeks from onset to the start of this study. Moxibustion was applied at 合谷(LI14), 外關(TE5), 曲池(LI11), 太衝(LIV3), 懸鍾(絶骨,G39), 足三里(S36) in hemiplegic upper and lower extremity, once a day for 6 weeks. The effect of treatment on functional recovery was assessed using the Functional Independence Measure scale. Statistical significance was achieved if the probability was less than 5%(p<0.05). Result : These 2 groups had comparable clinical characteristics; sex, age, lesion, and pre-treatment FIM score. After 6 weeks, patients in the moxibustion group performed better on FIM. The differences were significant(P=0.001). Conclusion : These results suggest that moxibustion is an effective treatment for functional recovery in stroke patients.

Crossing Dynamics of Leader-guided Two Flocks

이상희,Lee, Sang-Hee The Korea Society for Simulation 2010 한국시뮬레이션학회 논문지 Vol.19 No.3

In field, one can observe without difficulties that two flocks are intersected or combined with each other. For example, a fish flock in a stream separates into two part by obstacles (e.g. stone) and rejoins behind the obstacles. The dynamics of two flocks guided by their leader were studied in the situation where the flocks cross each other with a crossing angle, ${\theta}$, between their moving directions. Each leader is unaffected by its flock members whereas each member is influenced by its leader and other members. To understand the dynamics, I investigated the order parameter, ${\phi}$, defined by the absolute value of the average unit velocity of the flocks' members. When the two flocks were encountered, the first peak in ${\phi}$ was appeared due to the breaking of the flocks' momentum balance. When the flocks began to separate, the second peak in ${\phi}$ was observed. Subsequently, erratic peaks were emerged by some individuals that were delayed to rejoin their flock. The amplitude of the two peaks, $d_1$ (first) and $d_2$ (second), were measured. Interestingly, they exhibited a synchronized behavior for different ${\theta}$. This simulation model can be a useful tool to explore animal behavior and to develop multi-agent robot systems.

RF Plasma CVD 에 의한 다이아몬드 박막의 합성

이상희,이병수,김영봉,김보열,이종태,우호환,박상현,이덕출 ( Sang Hee Lee,Byoung Soo Lee,Young Bong Kim,Bo Youl Kim,Jong Tae Lee,Ho Whan Woo,Sang Hyun Park,Duck Chool Lee ) 한국전기전자재료학회 1997 한국전기전자재료학회 추계학술대회논문집 Vol.1997 No.1

계류 척수 증후군에 의한 야뇨증과 신부전 1례

이상희,이윤경,김동언,김영훈,김소영,한승훈,Lee Sang Hee,Lee Yoon Kyung,Kim Dong Un,Kim Young Hoon,Kim So Young,Han Seung-Hoon 대한소아신장학회 2002 Childhood kidney diseases Vol.6 No.1

저자들은 야뇨증을 주소로 내원한 12살 남아에서 천추 부위의 피부 보조개를 발견하여 정밀 검사를 통한 계류 척수 증후군을 확인하였고 이에 따른 신경원성 방광과 만성 신부전을 경험하였기에 문헌 고찰을 통한 증례 보고를 하는 바이다. The tethered cord syndrome is characterized by sensory and motor disturbances of the lower extremities and incontinence. We report a 12-year-old boy with sacral lipoma and a tethered cord syndrome, whose chief complaint is enuresis. This complication of tethered cord syndrome was most likely due to a renal failure and hydronephrosis secondary to a neurogenic bladder. Thus, the spinal lesions have to be considered in patients with enuresis.(J Korean Soc Pediatr Nephrol 2002 ;6 : 109-13)

영상 압축 부호화를 위한 DCT영역에서의 예측 부호화 방법

이상희,김재균,Lee, Sang-Hee,Kim, Jae-Kyoon 대한전자공학회 1998 電子工學會論文誌, S Vol.s35 No.8

Intra-frame video compression, which cannot make use of temporal predictions, requires much higher bit rates compared with inter-frame schemes. In order to reduce bit rates, intra-frame predictive coding methods in DCT domain have been studied especially within the framework of the MPEG-4 video coding standard currently being developed. In this paper, we propose novel intra-frame predictive coding methods in DCT domain with the marginal complexity increase over the conventional methods . The proposed methods consist of a DC coefficient prediction method and two AC coefficient prediction methods. The proposed methods consist of a DC coefficient prediction method and two AC coefficient prediction methods. The proposed DC coefficient prediction method makes it possible to adaptively select the prediction directions without overhead bits, by comparing gradients of DC coefficients from neighboring blocks. As the AC coefficient prediction methods, first, we present an effective method which can improve the prediction directions of the MPEG-4 scheme by considering the DC coefficient of the current block to be coded. And, we present another effective method that decision on the prediction is carried out for each AC coefficient. Simulation results show that substantial bit savings can be achieved by the proposed methods. 시간 방향 예측을 사용할 수 없는 Intra-프레임 부호화는 Inter-프레임 부호화에 비해 상당히 높은 비트율을 필요로 한다. 이러한 Intra-프레임의 비트율 감축을 위하여 최근 표준화가 진행중인 MPEG-4에서는 DCT영역에서의 화면내 예측 부호화 방식에 관한 연구를 진행해 왔다. 본 논문에서는 기존 방식들에 비해 약간의 복잡도가 추가된 DCT 영역에서의 화면내 예측 부호화 방식들을 제안한다. 제안 방식은 DC 계수 예측 방식과 2가지의 AC 계수 예측 방식들로 구성된다. DC 계수 예측 방식으로는, 이웃 블록들의 DC 계수간 기 울기를 이용하여 부가 정보없이 선택적으로 예측 방향을 결정하는 방식을 제안한다. AC 계수 예측 방식으로는, 현재 블록의 DC 계수를 고려하여 MPEG-4의 AC 계수 예측 방향을 개선한 방식을 제안하며, 또한, AC 계수 단위로 예측 여부를 결정하는 방식을 제안한다. 실험을 통하여 제안 방식에 의해 상당한 비트율 감축을 얻을 수 있음을 보인다.