미나리추출물이 사염화탄소에 의한 마우스 간손상에 미치는 영향

이상일(Sang-ll Lee),박용수(Yong-Su Park),조수열(Soo-Yeul Cho) 한국식품영양과학회 1993 한국식품영양과학회지 Vol.22 No.4

미나리의 해독작용 기전을 구명하기 위하여 미나리 n-butanol추출분획을 실험동물에 전처치한 다음, 간독 소인 사염화탄소를 투여하고 급성 간손상의 정도를 확인함과 동시에 조직손상과 관련된 free radical 생성계 및 해독계효소의 활성에 미치는 영향을 검토하였다. 사염화탄소를 투여하였을 때 혈청 ALT 활성 및 간조직과산화지질의 함량이 대조군에 비하여 현저히 증가되었으나, 미나리추출물을 전처치하므로서 증가현상이 유의하게 억제되었다. 한편 미나리추출물 (50㎎/㎏)을 실험동물에 투여하였을 때, 간조직의 free radical 생성계 효소인 aniline hydroxylase의 활성 및 free radical 해독계 효소인 catalase 및 glutathione S-transferase의 활성과 간조직 glutathione의 함량은 유의한 변동을 나타내지 않았다. 그러나 free radical 생성계 효소의 일종인 간조직 xanthine oxidase의 활성은 미나리추출물의 투여량과 투여기간에 반비례하여 감소되었으며, 50㎎/㎏ 및 5일간 투여군에서 유의한 억제현상을 관찰할 수 있었다. 시험관내에 미나리 추출물의 첨가농도를 증가시켜가면서 간조직 xanthine oxidase의 활성을 측정하였을 때 효소의 활성은 변동이 없었다. 이상의 실험성적들을 종합해 볼 때, 미나리 n-butanol 추출분획은 free radical 생성계 효소인 간조직 xanthine oxidase의 활성을 조절하므로서 사염화탄소에 의한 간손상을 보호해줄 것으로 사료되어진다. The present work was undertaken to investigate the protective mechanism of Oenanthe javanica n-butanol extract on the carbon tetrachloride-induced hepatotoxicity in mice. It was observed that a striking enhancement of serum alanine aminotransferase and hepatic lipid peroxide content after carbon tetrachloride administration were markedly decreased by the pretreatment of Oenanthe javanica extract for 5 days. It was also observed that the hepatic aniline hydroxylase, catalase, glutathione S-transferase activity and glutathione content were not changed by the injection of Oenanthe javanica extract for 5 days. Whereas, hepatic xanthine oxidase activity was inhibited by the treatment of Oenanthe javanica extract for 5 days. After treatment with Oenanthe javanica extract, xanthine oxidase activity was decreased with dose and time-dependent manner as compared to control group. However, hepatic xanthine oxidase activity was not affected by the addition of Oenanthe javanica extract in vitro. These results suggest that the inhibition of hepatic xanthine oxidase activity by the injection of Oenanthe javanica extract is believed to be a possible protective mechanism for the carbon tetrachlorideinduced hepatotoxicity in mice.

마우스의 아세트알테히드 대사에 미치는 인삼 부탄을 분획 (分劃)의 영향

이상일(Sang ll Lee),최종원(Chong Won Choi),허청(Keun Huh),박종민(Chong Min Park) 영남대학교 자원문제연구소 1986 資源問題硏究 Vol.5 No.-

반응표면분석에 의한 발효 청미래덩굴(Smilax china L.) 잎 열수 추출조건의 최적화

김재원 ( Jae Won Kim ),이상일 ( Sang Ll Lee ),이예경 ( Ye Kyung Lee ),양승환 ( Seung Hwan Yang ),김순동 ( Soon Dong Kim ),서주원 ( Joo Won Suh ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.5

In this study, we investigated the contents of total polyphenol (TP), total flavonoid, and absorbance at 475 nm (OD475) which may produced in solid-fermented leaf of Smilax china L. by Aspergillus oryzae as a new functional components with reddish brown color, contents of water soluble substance (WSS), electron donating ability (EDA), Hunter L*, a*, b* values, sensory overall acceptability (OA) and also, the inhibitory activities (XOI and AOI) against partial purified xanthine oxidase (XO) and aldehyde oxidase (AO) from rabbit liver which were well known to relate the gout, and alcoholic liver disease, respectively in order to optimize water extraction using response surface methodology (RSM). All the R2 values of the second-order polymonials ranged from 0.85 to 0.98, except for the EDA (0.69) and the XOI (0.78). However, the activities of the EDA and XOI were relatively high in the lower concentration of the fermented Smilax china L. leaf. The effects on the water extraction were highest in the concentration, among the dependent variables, and showed significant differences at the 1% level in the TP, TF and WSS contents and the a*, b* and OD475 values, but the OA showed significant differences at the 5% level. The optimal values of AOI, which was the most important functionality in the Smilax china L. that was predicted via RSM, were 59.48% at the 2.19% concentration, a 90.02°C extraction temperature and a 4.03 minute extraction time (R2: 0.93, p<0.007). The ranges of all the dependent variables of the optimal water extraction were 1.6~1.8% for the concentration, 83~93°C for the temperature and 3.4~4.4 minutes for the extraction time; and the optimal water extraction conditions were a 1.7% concentration, an 88°C extraction temperature and a 3.9-min extraction time.

흰쥐에서 에탄올이 유도한 간 Aniline Hydroxylase 활성에 미치는 인삼의 영향

허근(Keun Huh),이상일(Sang ll Lee),임상규(Sung Kyu Lim),최종원(Chong Won Choi),박종민(Chong Min Park) 영남대학교 자원문제연구소 1986 資源問題硏究 Vol.5 No.-

Effect of Garlic on the Hepatic Xanthine Oxidase Activity in Rats

허근(Keun Huh),이상일(Sang ll Lee),박종민(Jong Min Park) 영남대학교 자원문제연구소 1986 資源問題硏究 Vol.5 No.-

Purine 대사과정에 미치는 마늘 수침액의 영향

허근(Keun Huh),이상일(Sang ll Lee),김석환(Seok Hwan Kim),박종민(Jong Min Park) 영남대학교 자원문제연구소 1987 資源問題硏究 Vol.6 No.-

3상 일체화된 자속구속형 전류제한기의 동작특성

이나영(Na-Young Le),최효상(Hyo-Sang Choi),정수복(Soo-Bok Jeong),이상일(Sang-ll Lee),남긍현(Gueng-Hyun Nam),임성훈(Sung-Hun Lim) 한국조명·전기설비학회 2006 한국조명·전기설비학회 학술대회논문집 Vol.2006 No.5월

Superconducting fault current(SFCLs) are expected to improve not only reliability but also stability of real power systems. The analysis on the single line-to-ground fault current of the integrated three phase flux-lock type SFCL, which consists of three flux-lock reactor wound on an iron core in each single phase and three YBCO thin films, was investigated in current limiting operating characteristics. We compared additive polarity winding with the subtractive one in the flux lock reactor. Its turns ratio each phase between the primary and the secondary coils is 63:42. When a single line-to-ground fault occurred in any phase, the peak value of line current in the fault phase of the additive polarity winding increased up to 31.44[A] during first-half cycle. On the other hand, the peak value in the subtractive polarity winding increased up to 81.77[A] under the same conditions. This is because the current flow between the primary and the secondary windings becomes to be additive or subtractive in each winding direction. We confirmed that the current limiting behavior in the additive polarity winding was more effective for a single-line-to ground fault.

흰쥐의 肝 Xanthine Oxidase 활성에 미치는 Ethionine의 影響

尹鍾國(Jeog Gook Yoon),申重圭(Joong Ku Sìn),李相日(Sang ll Lee) 한국환경보건학회 1990 한국환경보건학회지 Vol.16 No.2

Liver and serum xanthine oxidase(XOD) activity were determined in rats treated with DL-ethionine. COIlcomitantly, the enzyme activity was compared with that of rats treated with CC14‘ actinomycin D and those fed a protein depleted diet. The activity of XOD in liver was inhibited by injection ethionine to the rats. But. there were no differences in activity of serum XOD between control group and ethionine-treated rats‘ And the pattern of changes in enzyme activity of serum and liver in ethionine-treated rats. was simiJar with actinomycin D treated rats or those fed a protein depleted diet. On the other hand , the activity of XOD was rather elevated both in serum and liver by injection of CC14 to rats

식이성 Tungstate가 사염화탄소 투여에 의한 흰쥐 간 손상에 미치는 영향

윤종국(Chong-Guk Yoon),박해숙(Hae-Sook Park),이상일(Sang-ll Lee) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.22 No.6

흰쥐를 tungstate첨가식이 및 tungstate를 첨가시키지 않은 표준식으로 약 1개월간 성장시킨 후 간 및 혈청 xanthine oxidase활성을 측정한 결과, xanthine oxidase활성이 표준식이군 보다 tungstate첨가식이군이 현저히 감소됨으로서 tungstate첨가식이군이 xanthine oxidase결핍 실험동물모델로 확인되었다. 이때 CCI₄투여 (0.1㎖/100g body wt., 1일 간격 두번)로 인한 간무게 및 혈청 alanine aminotransferase 활성 증가율과 간세포 중 단백질 감소율은 tungstate첨가식이군이 표준식이군 보다 낮게 나타났다. 또한 병리조직검사 소견에서도 tungstate첨가식이군이 표준식이군 보다 간손상의 정도가 낮게 나타났다. 따라서 tungstate식이군이 표준식이군 보다 간손상이 경미하게 나타남을 확인할 수 있었다. 한편 간조직 중 xanthine oxidase의 type O 활성은 대조군 및 CCI₄투여군 모두 tungstate첨가식이군이 표준식이군 보다 현저히 감소되었다. 그리고 간 microsomal aniline hydroxylase활성은 tungstate 식이군이 표준식이군 보다 높게 나타났으며, CCI₄투여로 인한 본 효소의 감소율 역시 tungstate 식이군이 표준식이군 보다 높게 관찰되었다. 또한 간조직 중 glutathione함량과 이의 포함효소인 glutathione S-transferase활성과 catalase, glutathione peroxidase, superoxide dismutase활성변동에 있어서는 대조군 및 CCI₄투여군 모두 표준식이군과 tungstate첨가식이군간에 대체적으로 통계적인 차이를 볼 수 없었다. 이상 실험결과로 보아 식이성 tungstate에 의한 CCI₄로 유도된 간손상의 감소현상은 xanthine oxidase의 활성이 tungstate에 의해 억제되므로써로 이로 인하여 인하여 oxygen free radical의 생성율이 저하된 때문에 나타난 결과로 생각된다. To evaluate the role of xanthine oxidase in liver damage by CCI₄, a group of rats were fed tungstate for a month, which suppressed the activities of xanthine oxidase in serum and liver. Control group of rats were fed standard diet without tungstate. Liver damage was induced both in tungstate fed and control groups by two intraperitoneal injections of CCI₄ at the level of 0.1㎖/100g body weight at intervals of 24 hours. Increases in the levels of serum alanine aminotransferase by CCI₄ were significantly smaller in tungstate fed rats than in control rats. Concomitantly, histopathologic changes were less in tungstate fed rats than in control ones. In rats either treated with CCI₄ or not, hepatic type O xanthine oxidase activities were remarkably reduced by tungstate feeding. Hepatic aniline hydroxylase activities, were higher in rats fed tungstate than control rats when animals were not treated with CCI₄, but the enzyme activities were lower in tungstate fed rats than control when they were treated with CCI₄. Neither tungstate feeding nor CCI₄ treatment caused any significant changes in hepatic glutathione contents, and activities of hepatic glutathione S-transferase, glutathione peroxidase and superoxide dismutase. It is concluded that xanthine oxidase reaction augment CCI₄ induced liver damage via oxygen free radical system.

지역인적자원개발 거버넌스

이상일 인제대학교 2009 仁濟論叢 Vol.24 No.1

The result of local human resources development(LHRD) depends on whether or not to construct ’good’ governance which is appropriate to local circumstances. In Korea, for the last 10 odd years, a variety of construction plans for LHRD have been presented, and some laws also have been consolidated. However, the governance issue of human resources development does not get along well yet at local levels. One of reasons why the governance of LHRD has not been fixed in Korea is due to insufficient efforts to investigate theoretically the governance of LHRD. Without sufficient theoretical investigation, approaches to construct the governance of LHRD may result in lots of trial and error. This paper aims to examine governance theories such as liberalism theory, network management theory, decentered theory, delegation theory, and to consider how these theories can be useful in constructing the governance of LHRD. This paper also tries to investigate theoretical analyses of Korea’s current system of LHRD with British and American governance experiences of LHRD