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- 저자 : 이봉재(Bong-Jae Lee) (검색결과 208 건)
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시험관내 항원 투여가 알레르기성 비염 환자의 비점막내 호산구 침윤과 접착분자의 발현에 미치는 영향
이봉재,신현수 (Bong Jae Lee,Hyun Soo Shin) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.2
We aimed to investigate the effect of in vitro allergen challenge on activation of eosinophils and expression of intercellular adhesion molecule-1(ICAM-1) in the epithelial and endothelial cells of the nasal mucosa of allergic rhinitics. Materials and methods . Inferior turbinate mucosal specimens which were obtained from 16 perennial allergic rhinitics and sensitized to D. farinae during conchotomy operations were used. Each mucosal specimen was divided into six pieces. The first two pieces were used as basic control group without culture, the second two pieces as culture-control group which was cultured with diluent only, and the last two pieces as antigen-challenge group cultured with D. farinae solution for 24 hours. Specimens were immunohistochemically stained using monoclonal anti- body against eosinophil cationic protein(EG1 for all eosinophils and EG2 for activated forms) and ICAM-1. Results .' Average counts of EG1 cells and EG2+ cells within 1 mm of lamina propria of nasal mucosa were 14 and 11 respectively in the basic control group, 17 and 19 in culture control group, and 16 and 16 in the antigen-challenge group. ICAM-1 expression on vessels was observed more than half of t,he vessels in the nasal mucosa and there was no significant difference between groups, however, its expression on the epithelial cells was most prominent in the antigen-challenge group (2.1 in basic control group, 3.3 in culture control group, and 6.2 in antigen-challenge group). Conclusion: Most of the eosinophils infiltrat,ing into the nasal mucosae out of vessels in allergic rhinitics are activated forms. Significant up-regulation of epithelial ICAM-1 expression by antigen-challenge suggests that ICAM-1 contributes to the transmigration of eosinophils from the subepithelial tissue into the epithelial cell layer and then finally into the nasal secretion.
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원전해체 시 콘크리트 구조물 절단을 위한 밀기형 절단장치 개발
이봉재,권용규,홍창동,이동원,민경남,Lee, Bong-Jae,Kwon, Yong-Kyu,Hong, Chang-Dong,Lee, Dong-Won,Min, Kyong-Nam 한국방사성폐기물학회 2020 방사성폐기물학회지 Vol.18 No.1
Pulling-type cutting devices, which use a diamond wire saw, have been used generally for cutting concrete structures. In this study, a pushing-type cutting device with a collection cover was developed by overcoming the disadvantages of pulling-type devices. In this device, dry or liquid methods can be selected to cool frictional heat. Operation and leakage tests of the dust generated during the dismantling of a concrete structure were carried out, confirming the suitable operation of the fabricated cutting device; the leakage rate was approximately 1.7%. For a conservative evaluation, the internal dose of workers was estimated in dismantling the core center part of biological shield concrete with a specific activity of 99.5 Bq·g<sup>-1</sup>. The committed effective dose per worker was 0.25 mSv. The developed cutting device contributed to reducing radioactive concrete waste and minimizing worker exposure due to its easy installation. Therefore, it can be utilized as a cutting apparatus for dismantling not only reinforced concrete structures but also radioactive biological shield concrete in nuclear power plant decommissioning efforts.
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이봉재,Lee, Bong-Jae 한국정신신체의학회 2008 정신신체의학 Vol.16 No.2
마취는 수술전 환자평가로부터 시작된다. 환자평가의 목적은 수술에 앞서 환자가 정신적으로나 신체적으로 최상의 상태에 있도록 하여 수술과정에 발생할 수 있는 이환율(morbidity)과 사망률(mortality)을 감소시키는데 있다. 환자의 정보를 수집하고 신체상태를 평가하여 마취계획을 세우기 위해서는 환자를 직접 만나야 한다. 수술전 환자방문은 안전과 효과적인면에 있어서 수술중 환자 관리만큼이나 중요하다. Every anesthetic begins with preoperative evaluation. This meeting of patient and anesthesiologist allows for an exchange of information, an assessment of physical status, and the formulation of an anesthetic plan. The aim is to have the patient in the best possible condition, both mental and physical, prior to surgery. The preoperative visit is as important as any aspect of introperative management in providing a safe, effective anesthetic.
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이봉재,박영웅,Lee, Bong-Jae,Park, Yeong-Ung 한국동위원소협회 2006 동위원소회보 Vol.21 No.4
A passive type radon monitor adopting two silicon PIN detector as radiation detector has been developed, manufactured and test-evaluated. A radiation signal processing circuit has been electronically tested and then the radiation detection characteristics of this instrument has been performance-tested by using reference radon concentration and a reference photon radiation field. As a result, in a electronic performance test, radiation signals from each detector were well observed in each signal processing circuit. The radiation detection sensitivity of this instrument after several test-irradiations to a Cs-137 gamma radiation source and a standard radon concentration appeared to be 1.37 cph/$\mu$Svh-1 and 1.66 pCi/L respectively. The developed radon monitor in this paper could be used conveniently in monitoring of radon concentration in buildings which population utilize in Korea.
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Branched-Chain 아미노산 Aminotransferase에 관한 연구(IV) 악하선의 Branched-Chain Amino Acid Aminotransferase에 관하여
이봉재,장양섭,이근배,Lee, Bong-Jae,Chang, Ryang-Sub,Lee, Keun-Bai 생화학분자생물학회 1980 한국생화학회지 Vol.13 No.4
한우의 악하선에 있는 branched-chain아미노산 aminotransferase [EC 2.6.1.6]의 분포 및 isozyme의 pattern을 관찰하였다. 효소의 활성도는 Ichihara and Koyama의 방법으로 측정하였으며 isozyme은 ammonium sulfate에 의한 침전 및 DEAE-cellulose column chromatography에 의하여 분리하였다. 이상과 같이 하여 다음과 같은 결론을 얻었다. 1. 악하선의 branched-chain amino acid aminotransferase 활성도는 흰쥐의 정상조직인 심장, 장관 및 췌장에 비하여 낮았다. 2. BCAT는 세포질, mitochondria 및 핵분획에서 발견되며, 세포질에 약 85%, mitochondria에 약 8%, 핵분획에 약 6%가 분포되어 있었다. 3. 기질에 대한 효소활성도는 L-leucine이 가장 높았다. 4. 세포질에 있는 이 효소를 부분 정제하여 isozyme의 pattern을 관찰하기 위하여 DEAE-cellulose column chromatography를 행한 결과 용출액인 인산염 완충액의 농도가 20mM에서 Enzyme I 만이 검출되었다. 5. 소의 악하선에는 BCAT의 isozyme인 Enzyme II 및 III은 발견할 수 없었다. The activity and isozyme pattern of branched-chain amino acid aminotromsferase (EC 2.6.1.6) in bull submaxillary gland have been studied. The activity of branched-chain amino acid aminotransferase was measured by the method of Ichihara and Koyama. Isozyme pattern of this enzyme was also examined by using of DEAE-cellulose column chromatography. The results obtained were as follows; 1. In the bull submaxillary gland, the aminotransferase activities for branched-chain amino acids are relatively low compared with those in rat tissues, i, e., intestine, pancreas, and heart. 2. This enzyme was localized in the cytosolic, mitochondrial and nucleic fraction. About 85% of the total activity was found in the cytosolic fraction, 8% in the mitochondrial fraction and 6% in the nucleic fraction. 3. Cytosolic fraction of bull submaxillary gland contained Enzyme I, but not Enzyme II and III, of branched chain amino acid aminotransferase Enzyme I was eluted by 20mM phosphate buffer from DEAE-cellulose column and catalyzed the transamination of all three branched-chain amino acids, 4. The chromatographic elution profile of the Enzyme I from the bull submaxillary gland was very similar with those of normal rat liver and brain.
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