PET/PTT/Rayon 스테이플 혼방직물의 역학적 특성

이대훈,최규락,나미희,차희철,Lee, Dae-Hoon,Choi, Kue-Lak,Na, Mi-Hee,Cha, Hee-Chul 한국섬유공학회 2012 한국섬유공학회지 Vol.49 No.2

The mechanical properties and strain at applied loads for PET/PTT (polyester/poly trimethylene terephthalate)/rayon staple blended fabrics, PET/rayon blended fabrics, and PET/rayon containing PU fabrics were studied. The result of the research shows that PET/PTT/rayon staple blended fabrics have higher bending and shear properties than other fabrics in cases regarding the weft direction. Elastic recovery above a 30% strain of PET/PTT/rayon staple blended fabrics is larger than that of other fabrics. PET/PTT/rayon staple blended fabrics are easily extensible at the small as well as the large deformation region.

미세 연소기 개발 (III) - 감광 유리를 이용한 마이크로 엔진의 제작 -

이대훈,박대은,윤준보,윤의식,권세진,Lee, Dae-Hoon,Park, Dae-Eun,Yoon, Joon-Bo,Yoon, Eui-Sik,Kwon, Se-Jin 대한기계학회 2002 大韓機械學會論文集B Vol.26 No.12

Micro engine that includes Micro scale combustor is fabricated. Design target was focused on the observation of combustion driven actuation in MEMS scale. Combustor design parameters are somewhat less than the size recommended by feasibility test. The engine structure is fabricated by isotropic etching of the photosensitive glass wafers. Electrode formed by electroplating of the Nickel. Photosensitive glass can be etched isotropically with almost vertical angle. Bonding and assembly of structured photosensitive glass wafer form the engine. Combustor size was determined to be 1 mm scale. Movable piston is engraved inside the wafer. Ignition was done by nickel spark plug which was electroplated with thickness of 40 ${\mu}{\textrm}{m}$. The wafers were bonded by epoxy that resists high temperature. In firing test due to the bonding method and design tolerance pressure buildup by reaction was not confirmed. But ignition, flame propagation and actuation of micro structure from the reaction was observed. From the result basement of design and fabrication technology was obtained.

형상 축소된 연소기의 열손실 및 소염해석 모델

이대훈,권세진,Lee, Dae-Hoon,Kwon, Se-Jin 대한기계학회 2002 大韓機械學會論文集B Vol.26 No.7

Down scaled combustor undergoes increased heat loss that results in incomplete combustion or quenching of the flame as a consequence. Therefore, effect of enhanced heat loss should be understood to design a MEMS scale combustion devices. Existing combustion models are inadequate for micro combustors because they were developed for analysis of regular scale combustor where heat loss can be ignored during the flame propagation. In this research a combustion model is proposed in order to estimate the heat loss and predict quenching limit of flame in a down scaled combustor. Heat loss in the burned region is expressed in a convective form as a product of wall surface area, heat transfer coefficient and temperature difference. Comparison to the measurements showed satisfactory agreement of the pressure and temperature drop. Quenching is accounted for by introducing a correlation of quenching parameter and heat loss. The present model predicted burnt fraction of gases with reasonable accuracy and proved to be applicable in thermal design of a micro combustor.

불등풀가사리 다당류의 분자량에 따른 이화학적 품질특성 및 피부 주름 개선 효과

이대훈,홍주헌,Lee, Dae-Hoon,Hong, Joo-Heon 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.5

본 연구는 불등풀가사리로부터 분리된 조다당류와 산가수분해된 다당류의 분자량에 따른 이화학적 품질특성과 항산화 활성 및 피부 주름 개선 효과를 확인하였다. 산가수분해 분자량별 다당류의 수율은 100 kDa 이상에서 29.6%로 가장 높았으며, 10 kDa 이하에서 10.4%로 가장 낮았다. 이화학적 특성으로 총당, uronic acid, sulfate 함량은 100 kDa 이상에서 각각 85.82%, 32.85 g/100g 및 39.04%로 조다당에 비해 증가하였다. 색도은 분자량이 증가함에 따라 L 값, a 값 및 b 값이 감소하였으며, 점도는 분자량이 높을수록 증가하였다. ORAC 활성은 100 kDa 이상에서 $180.07{\mu}M$을 나타내어 조다당 $34.70{\mu}M$에 비해 항산화활성이 우수하였다. L132 세포 사멸에 대한 보호효과는 $H_2O_2$ 처리 구간대비 분자량 100 kDa 이상($0.5-5{\mu}g/mL$)에서 87.34-103.85%로 세포 활성이 증가하여 세포 보호효과를 나타내었다. MMP-1 활성은 세포에 UVB를 조사한 후 분자량 100 kDa 이상 $0.05-0.5{\mu}g/mL$ 농도에서 69.67-100.87%의 저해 활성을 나타내었다. 또한, pro-collagen 생합성 역시 UVB를 조사한 후 분자량 100 kDa 이상 $0.05-0.5{\mu}g/mL$ 농도에서 64.91-77.80%의 합성능을 나타내어 농도 의존적으로 증가하는 것을 확인하였다. 따라서, 불등풀가사리 유래 산가수분해된 다당류의 분자량에 따른 주름 개선 효과를 통해 피부 항노화 기능성 소재로 다양하게 활용 가능할 것으로 사료된다. In this study, the physicochemical properties and anti-wrinkle effect of polysaccharides with different molecular weights from Gloiopeltis furcata were investigated. Crude polysaccharides were isolated by viscozyme treatment followed by ethanol precipitation and lyophilization. Crude polysaccharides were hydrolyzed by acid (0.1 N HCl) and the molecular weight fractions were generated by centrifugal filter (<10 kDa, 10 to 100 kDa, and 100 kDa>). The yield of polysaccharides with different molecular weight fractions was 8.4-39.6%. The major constituents in molecular weight fractions were total sugar (81.37-85.82%), uronic acid (27.89-32.85 g/100 g), sulfate (33.38-39.04%), and protein (0.35-3.16%) The L, a, and b value of the 100 kDa group were decreased, but viscosity increased. The oxygen radical absorbance capacity of the 100 kDa group at $180.07{\mu}M$ was the highest among groups. The protective effects of 100 kDa group at 0.5 and $5{\mu}g/mL$ against $H_2O_2$-induced cytotoxicity in L132 cell were 87.34% and 103.85%, respectively. The matrix metalloproteinase-1 activity of 100 kDa group decreased in a dose-dependent manner. The pro-collagen synthesis activity of 100 kDa group at $0.05-0.5{\mu}g/mL$ was 64.91-77.80%. The polysaccharides with different molecular weights from Gloiopeltis furcata investigated herein are useful as a potential candidate for cosmedical materials.

효소종류에 따른 불등풀가사리 유래 다당류의 이화학적 특성 및 생리활성

이대훈,홍주헌,Lee, Dae-Hoon,Hong, Joo-Heon 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.3

본 연구에서는 불등풀가사리 유래 다당류의 기능성식품소재로의 활용성을 향상시키고자 불등풀가사리에 5종의 상업용 효소를 처리한 다음 분리된 다당류의 이화학적 특성 및 생리활성을 조사하였다. 효소 분해 구간의 다당 수율은 52.8-66.4%로 무처리 구간 50.6%에 비해 유의적으로 증가하였다. 이화학적 특성으로 총당 및 단백질 함량은 각각 71.04% 및 7.22%, uronic acid 및 sulfate 함량은 23.18 g/100 g 및 28.27%로 효소 분해를 통해 증가하였다. DPPH radical 소거활성 및 FRAP에 의한 항산화 활성은 23.10% 및 $218.50{\mu}M$을 나타내어 무처리 구간에 비해 항산화 활성이 우수하였으며, L132 세포 사멸에 대한 보호효과는 viscozyme 효소처리 구간($1{\mu}g/mL$)에서 $H_2O_2$를 처리한 구간 대비 세포 보호효과는 85.64%로 세포 활성이 증가하여 높은 세포 보호효과를 나타내었다. NO 생산량은 viscozyme 효소 처리구간 $5{\mu}g/mL$ 농도에서 $32.13{\mu}M$ 함량을 나타내어 LPS 대비 90% 높은 생성량을 나타내었으며, 4종의 암세포(A549, SNU719, HeLa 및 MCF7) 생존율은 $25{\mu}g/mL$농도에서 각각 69.57%, 61.06%, 52.74% 및 68.64%의 유의적으로 낮은 암세포 생존율을 나타내었다. 따라서 불등풀가사리의 효소 분해를 통해 다당의 이화학적 특성 및 생리활성이 향상됨에 따라 기능성 식품소재 로 다양하게 활용 가능할 것으로 사료된다. In this study, the biological activities and physicochemical properties of polysaccharides from Gloiopeltis furcata were investigated. Polysaccharides were isolated by enzymes treatment (celluclast, flavourzyme, papain, termamyl, viscozyme) followed by ethanol precipitation and lyophilization. The yield of polysaccharides by enzymes treatment group were 52.8-66.4%. The major constituents in viscozyme treatment group were total sugar (71.04%), protein (7.22%), uronic acid (23.18 g/100 g), and sulfate (28.27%), respectively. The DPPH radical scavenging activity and ferric reducing antioxidant potential of the viscozyme treatment group at 5 mg/mL were 23.10% and $218.50{\mu}M$, respectively. The protective effects against $H_2O_2$-induced cytotoxicity in L132 cell of viscozyme treatment group at $1{\mu}g/mL$ was 85.64%. The viscozyme treatment group increased the production of nitric oxide (NO) in a dose-dependent manner. The antitumor activity of viscozyme treatment group (at $25{\mu}g/mL$) in A549, HeLa, SNU719 and MCF7 was 69.57%, 52.74%, 61.06% and 68.64%, respectively. All of data showed that the biological activities and chemical characteristics of enzymes treatment group are higher than that of the control group. The polysaccharides isolated from Gloiopeltis furcata investigated herein are useful as functional materials agents.

파일럿 안정화 제트버너의 예혼합 화염이 미연가스 영역 난류특성에 미치는 영향

이대훈,권세진,Lee, Dae Hoon,Kwon, Sejin 대한기계학회 1999 大韓機械學會論文集B Vol.23 No.9

Comparisons of measured turbulence properties in the unburned gas region of turbulent premixed flame stabilized by pilot flame, in cases of combusting and non-combusting flow conditions, are presented. Methane-air premixed jet at fuel equivalence ratio of 0.6 and 1.0 and Reynolds number of 7,000 was diagnosed using two-color laser velocimeter to obtain turbulence statistics. Same set of measurements was repeated at 21 locations within the unburned gas region of both combusting and non-combusting conditions. Velocity data were analyzed to evaluate the spatial distribution of turbulence properties including Reynolds stress, probability densities, joint probability densities and auto correlations. Contrary to assumptions of current theoretical models, significant influence of flame was observed in every property that was studied in the present investigation. The effective viscosity increased ten-fold when flame was on from cold flow values. The effect of mixing on joint probability as well as in turbulence intensity was suppressed by the flame. The measurements suggest that common assumptions of premixed flame model may result in sizable error in prediction of flame length and temperature distribution in near-field.

정적 연소실에서의 열 손실 해석 모델

이대훈(Dae Hoon Lee),권세진(Sejin Kwon) 한국연소학회 2001 한국연소학회지 Vol.6 No.1

우렁쉥이(Halocynthia roretzi) 껍질로부터 분리된 다당류의 면역증강 효과

이대훈(Dae-Hoon Lee),홍주헌(Joo-Heon Hong) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.5

본 연구에서는 우렁쉥이 껍질 유래 다당류의 기능성식품 소재로의 활용 가능성을 확인하기 위하여 조다당류의 분리 및 정제를 통해 획분을 얻었으며 다양한 면역증강 효과를 확인하였다. 건조된 조다당류를 DEAE-sepharose CL-6B를 이용하여 크로마토그래피한 후 총당 및 uronic acid 함량을 분석한 결과, 증류수로 용출되는 비흡착 획분(APF-Ⅰ, fraction No. 11~17)과 흡착된 후 NaCl 용액에 의해 용출되는 획분(APF-Ⅱ, fraction No. 22~37)을 얻었다. 정제된 APF-Ⅰ 및 APF-Ⅱ의 이화학적 특성으로 총당 함량은 각각 66.62%, 27.03%, uronic acid 함량은 47.53%, 15.87%, hexosamine 함량은 16.62%, 46.79% 및 단백질 함량은 2.43%, 4.94%로 나타났다. APF-Ⅰ 및 APF-Ⅱ에 대해 RAW 264.7 세포에 처리하여 독성을 평가한 결과 5 μg/mL 농도까지 유의적으로 세포사멸이 나타나지 않아 세포독성이 없음을 확인할 수 있었다. Nitric oxide 생산량은 APF-Ⅰ이 5 μg/mL 농도에서 22.23 μm 함량을 나타내어 LPS 대비 73.48%로 타 구간에 비해 높은 생성량을 나타내었으며, cytokine 생성량(TNF-α 및 IL-6) 또한 APF-Ⅰ 5 μg/mL 농도에서 각각 LPS 대비 104%, 100.1%를 나타내어 LPS와 유사한 생성량을 나타내었다. Polymerase chain reaction을 통한 면역관련 유전자 발현 분석 결과, iNOS, COX-2, TNF-α, IL-6에서 APF-Ⅰ 구간에서 LPS 처리군 보다 높은 발현량을 나타내어 면역증강을 목적으로 한 기능성식품 개발에 활용 가능하다고 사료된다. In this study, the immune-enhancing effects of purified polysaccharides from ascidian (Halocynthia roretzi) tunic were investigated. Crude polysaccharides (AP) were isolated by enzyme extraction (neutrase, 60°C, 15 h), ethanol precipitation, and lyophilization. In addition, crude polysaccharides were further fractionated into unabsorbed fractions (APF-Ⅰ, fraction No. 11∼17) and absorbed fractions (APF-Ⅱ, fraction No. 22∼37) by DEAE-sepharose CL-6B column chromatography in order to isolate immune regulating polysaccharide. The major constituents in APF-Ⅰand APF-Ⅱ were total sugar (66.62% and 27.03%), uronic acid (47.53% and 15.87%), hexosamine (16.62% and 46.79%), and protein (2.43% and 4.94%), respectively. APF-Ⅰ increased production of nitric oxide (NO) and cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 in a dose-dependent manner. The mRNA expression levels of inducible NO synthetase, cyclooxygenase-2, TNF-α, and IL-6 were markedly increased as determined by polymerase chain reaction analysis. The above data led us to conclude that macrophage activation of purified polysaccharides was higher than that of crude polysaccharides. The polysaccharides isolated from ascidian tunic investigated herein are useful as natural immune enhancing agents.

추출조건에 따른 오만둥이 유래 다당류의 이화학적 특성 및 항산화 활성

이대훈 ( Dae Hoon Lee ),홍주헌 ( Joo Heon Hong ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.2

오만둥이 유래 다당류의 효율적인 추출공정을 확립하기 위하여 추출조건에 따른 이화학적 특성 및 항산화활성을 조사하였다. 다당류의 추출수율은 효소처리추출에서 11.90%로 가장 높았으며, 열수추출이 8.60%로 가장 낮았다. 다당류의 유용성분인 uronic acid, hexosamine 및 sulfate-glycosaminoglycans 함량은 효소처리방법에서 각각 100 g당 10.86 g, 21.40 g 및 33.78 g으로 가장 높게 분석되었다. 다당류의 총아미노산 함량은 열수추출이 167.06 mg/100 g으로 가장 적었으며, 효소처리추출에서 808.51 mg/100 g로 가장 많이 함유되어 있었고 무기질 함량은 P>Ca>Mg>K 순이었다. 다당 류의 FT-IR 스펙트럼은 3,300~3,400 cm -1에서 당고리의 전형적인 O-H stretching 진동이 관찰되었으며, 2,900 cm -1에서 C=H stretching 진동이, 1,650 cm -1에서 C=O, 820~850 cm -1 에서는 C-O-S peak band를 나타내어 황산기가 존재하고 있었으며, 열분해 특성 분석결과 220~370에서 최대의 무게 감소를 보여주었고 열분해 온도는 대략 300 정도로 열적 안정성이 우수하였다. ORAC 및 FRAP에 의한 환원력은 효소처리추출에서 각각 215.38 μmole TE/g 및 295.07 mM로 항산화 활성을 우수하였으며, DPPH radical, 환원력 및 tyrosinase 저해 활성은 효소처리방법에서 각각 36.52%, 0.264 및 28.56%로 분석되어 열수추출 및 가압추출에 비해 우수함을 확인하였다. In this study, we extracted polysaccharides from Styela plicata, and investigated to determine its physicochemical properties and antioxidant activities. The yields of hot water extract (SHE), autoclave extract (SAE), and enzyme extract (SEE) were 8.6, 10.4, and 11.9%, respectively. The uronic acid contents were 10.86 g/100 g (SEE), 6.13 g/100 g (SAE) and 3.12 g/100 g (SHE), respectively. The hexosamine and sulfate-glycosaminoglycans contents of SEE was highest of 21.40 g/100 g, 33.78 g/100 g respectively. Composition of amino acids was mainly leucine, valine, serine and threonine. With regard to mineral content, P was the highest in all samples, followed by Ca>Mg>K, and Fe contents. The spectrum of FT-IR showed wavenumber at 3,300~3,400 cm-1, 2,900 cm-1, 1,650 cm-1, and 820~850 cm-1 representing the polysaccharide. The decomposition temperature was in the range of 300 and showed relatively good enough for their thermal stabilities. The oxygen radical absorbance capacity (ORAC), FRAP activity and ferrous ion chelating effect of SEE were 215.38 μmole TE/g, 295.38 μM, and 27.79%, respectively. Electron donating abilities, reducing power and tyrosinase inhibition activity increased with an increase in extract concentration.

플라즈마의 대기오염 방지 기술 적용 사례와 전망

이대훈(Dae Hoon Lee),김관태(Kwan-Tae Kim),송영훈(Young-Hoon Song),강홍재(Hongjae Kang),김유나(Youna Kim),송호현(Hohyun Song),이희수(Heesoo Lee),최정안(Jungan Choi) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11