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高磁氣場處理가 벼 幼苗生長과 培養細胞 再分化 및 蛋白質 二次元 電氣泳動패턴에 미치는 影響
Soon Tae Kwon(權純泰),Sei Myoung Oh(吳世明) 한국육종학회 1994 한국육종학회지 Vol.26 No.1
Responses of rice seeds and cultured cells subjected to high magnetic field stress ranging from 0.5 kilogauss(k gauss) to 4.0 k gauss were determined. The growth of roots exposed to magnetic field at 4.0 k gauss was stimulated by 8.8 and 14% over that of the control, whereas shoots inhibited by 7.0 and 10% in dry and imbibe treatment, respectively. Treatments of magnetic fields at 1.0 and 4.0 k gauss significantly increased the regeneration ability of roots from the cultured cells up to 60.7 and 63.7%, respectively, as compared with untreated control showing 50.1% at 30 days after treatment. Some increased and newly synthesized polypeptides, particularly the size of 22.3 kilodalton and pI 7.0, in the cells exposed to magnetic field of 4.0 k gauss were identified using two dimentional polyacrylamide electrophoresis, demonstrating that cultured rice cells respond to high magnetic field stress by changing their protein patterns.
방사선과 자외선에 대한 고추 식물체 및 배양세포의 생장반응과 Capsidiol 생합성 유전자의 발현 차이
안정희,김재성,정정학,오세명,권순태,An, Jung-Hee,Kim, Jae-Sung,Jeong, Jeong-Hag,Oh, Sei-Myoung,Kwon, Soon-Tae 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2
Differential responses of red pepper plant and cultured cells to enhanced ${\gamma}$-ray($^{60}$ Co) and ultraviolet(UV) stress were investigated. In seed treatment, 1 Gy of ${\gamma}$-ray increased seedling dry weight up to 19.1%, but 50 Gy treatment markedly ingibited seed germination and subsequent growth of seedling. UV treatment to seed did not change the germination ability of seeds and the growth of seedlings regardless of duration of UV treatment until 24 hrs. In case of UV treatment to seedlings, plant injury was seriously progressed even after the seedlings were returned to no UV condition, and eventually all the leaves showed chlorosis by the stress. However, progress of plant injury by ${\gamma}$-ray stress slower than that caused by UV stress, and even at the high dose of ${\gamma}$-ray 50 Gy, did not caused the cholrosis of stressed plant leaf. Amount of electrolytes leakage from plant leaf by UV treatment for 24hrs was increased up to 28.8 folds in comparison with untreated control, whereas that of 50 Gy of ${\gamma}$-ray was increased only 1.2 folds. UV stress induced the production of capsidiol, antimicrobial phytoalexin, by activation of gene expression involved in capsidiol biosynthesis, such as sesquiterpene cyclase and cyclase and cytochrome P450 hydroxylase in the leaf and cultured cell, but ${\gamma}$-ray stress induced neither the production of capsidiol nor expression of the genes.
권오흔,오세명 安東大學校 農業科學技術硏究所 1998 農業科學技術 硏究論文集 Vol.5 No.-
본 시험은 참당귀의 교잡육종을 위한 기초자료를 얻고자 참당귀 6개 주산지의 지방종에 대하여 수량과 생육특성을 비교하고 각 형질간의 상관관계를 조사하였으며 그 결과를 요약하면 다음과 같다. 지상부 생육에서 出?期는 5월 1~8일로 '봉화종'보다 '인제종'과 '무주종'이 6~7일정도 빨랐고 草長은 '봉화' '무주종'이 '태백종'보다 6.3~5.1cm 컸으며,葉數는 지방종간에 차이가 없었다. 추대는 6월 15일부터 시작하여 6월 15일~7월 15일 사이에 가장 많았으며, '인제종', '울진종' 및 '태백종'은 매우 낮은 추대율을 나타내었다. 各 지방종의 지하부 생육은 根長이 45.3~52.2cm 범위였고 根徑은 45.6~51.1mm로 '무주종'이 다소 컸으나 모두 차이는 인정되지 않았다. 株當生根重은 227~374g으로 '무주종', '태백종'이 다소 높게 나타났으나 유의성이 없었고, 10a당 乾根收量은 '태백종','인제종','울진종'이 '봉화종'보다 各各 288%, 260%, 244%로 다른 계통보다 많았다. 참당귀의 수량에 가장 크게 관여하는 형질은 추대율로서 고도에 負의 相關關係를 나타내었고 근형질 중에서는 근장이 正相關 關係를 보였다. 추대율은 초장과 正相關을 根長과는 負相關關係를 나타내었다. This study was conducted to obtain basic information on crop breeding for developing bolting resistant variety of Angelica gigas N which has been subjected to severe yield reduction resulted from flower-stalk initiation during the growth. Some agronomic characteristics such as plant height, number of leaves, emergence date and bolting rates of 6 local cultivars were investigated together with root characteristics and yields. Results obtained are summarized as follows. Emergence date of cultivars investigated in this experiment were from May 1 to May 8. Emergence date of Ponghwa-cultivar was May 8, and that of Inje, Muju-cultivar were May 1 and May 2, respectively. All cultivars except Ulchin-cultivar bolted on June 15. Of 6 cultivars tested, Inje, Ulchin and Taebaek-cultivar were appeared to be low-bolting cultivars showing 1.6%, 2.0%, and 9.0% bolting rates, respectively, whereas Ponghwa and Muju-cultivar were high-bolting cultivar showing more than 40% bolting rate. Root yields based on dry weight were higher in Taebaek(535kg/10a), Inje(483kg/10a), Ulchin(454kg/10a), Pyeongchang, Muju and Ponghwa-cultivar in order. Based on the analysis of correlation coefficients among various growth characteristic of Angelica gigas N root yield show significantly negative correlation with bolting rate suggesting that bolting is definitively influence on root quality and yield. Howerer, bolting rate showed positive correlation with plant height and negative correlation with root length.