임플란트 지대주 나사에 요구되는 물리적 성질

오동준 ( Dong Joon Oh ) , 한명주 ( Myung Joo Han ) , 정재헌 ( Chae Heon Chung ) 조선대학교 구강생물학연구소 2005 Oral Biology Research (Oral Biol Res) Vol.29 No.3

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BCAR3 Activates the Estrogen Response Element through the PI3-kinase/Akt Pathway in Human Breast MCF-12A Cells

Myung-Ju Oh(오명주),Joo-Yeon Ha(하주연),Byung H. Jhun(전병학) 한국생명과학회 2022 생명과학회지 Vol.32 No.11

Breast cancer anti-estrogen resistance 3 (BCAR3)는 유방암에서 항에스트로겐 내성을 유도하는 유전자들 중의 하나로 발견되었다. 우리는 이미 BCAR3가 c-jun, activator protein-1, serum response element의 promoter 등을 활성화하는 것을 보고하였다. 본 연구에서 우리는 정상 유방세포인 MCF-12A에서 estrogen response element (ERE) 활성에서의 BCAR3의 기능을 조사하였다. BCAR3의 발현이 ERE를 활성화하는 것을 발견하였다. 이 ERE 활성화는 17β-estradiol에 의해 더욱 증가하였고, 이는 항에스트론겐인 tamoxifen에 의해 억제되지 않았다. 다음으로 우리는 ERE 활성화를 이끄는 BCAR3의 신호전달 경로를 연구하였다. BCAR3에 의한 ERE 활성화는 phosphatidylinositol (PI) 3-kinase 경로 억제제인 LY294002와 AZD5363에 의해서는 억제되었으나, Mitogen-activated protein kinase 경로 억제제인 PD98059와 U0126에 의해서는 억제되지 않았다. ERE 활성화는 PI3-kinase의 catalytic subunit p110α와 Akt의 active mutant에 의해서는 유도되었고, 이 활성화는 추가적인 BCAR3에 의해서는 더욱 증가하지 않았다. 이러한 결과로부터 우리는 BCAR3가 PI3-kinase/Akt 신호전달경로를 통하여 ERE 활성화에 중요한 역할을 하는 것을 제시한다. Breast cancer anti-estrogen resistance 3 (BCAR3) has been identified as one of the genes that induces anti-estrogen resistance in breast cancer. We have previously reported that BCAR3 activates promoters of c-Jun, activator protein-1, and the serum response element. In this study, we investigated the functional role of BCAR3 in the activation of the estrogen response element (ERE) in normal human breast MCF-12A cells. Transient expression of BCAR3 induced ERE activation, which was further increased by 17β-estradiol treatment but was not blocked by the anti-estrogen tamoxifen. Next, we studied the signaling pathway of BCAR3 leading to ERE activation. BCAR3-mediated ERE activation was inhibited by LY294002 and AZD5363, inhibitors of the phosphatidylinositol (PI) 3-kinase pathway, but not by PD98059 and U0126, inhibitors of the mitogen-activated protein kinase pathway. ERE activation was induced by the catalytic subunit p110α of PI3-kinase or the active mutant of Akt, and this activation was not further increased by additional BCAR3 transfection. Based on these results, we propose that BCAR3 plays an important role in ERE activation through the PI3-kinase/Akt pathway in human breast MCF-12A cells.

중합효소연쇄반응법(Polymerase Chain Reaction, PCR)에 의한 남해안 양식산 어류로부터 Birnavirus의 검출

오명주,정성주,김영진,Oh, Myung-Joo,Jung, Sung-Ju,Kim, Young-Jin 한국어병학회 1999 한국어병학회지 Vol.12 No.1

복부팽만, 안구돌출 및 체색혹화의 외부증상을 대표적으로 나타내며, 세균 및 기생충과 같은 병원체 감염의 원인이 아닌 버나바이러스성으로 판단되어지는 남해안 양식산 넙치, 조피볼락, 농어를 대상으로 RT-PCR 법에 의한 체내 감염 바이러스 검출을 행하였다. IPNV 및 MBV만을 선택적으로 확인 검출 할 수 있으며 감염어체를 사용한 검출에서 배양 세포법(12/50)에 비하여 높은 검출 감도(46/50)를 나타내는 RT-PCR 진단법을 도입하였다. 아울러 우리나라 남해안 양식장에 버나바이러스의 감염이 폭넓게 진행되고 있음을 확인할 수 있었다. To detect birnavirus from cultured marine fish, RT-PCR assay was developed. This method was specific for aquatic birnaviruses that include IPNV Sp., IPNV Ab, IPNV VR-299 and MBV Y6. The birnavirus gene was detected (birnavirus positive samples detected 46/50) from clinical samples signed with abdominal distension and overall darkening even though the samples gave negative results in virus isolation (birnavirus isolate with CHSE-214 cell showed 12/50).

계란난황항체의 Edwardsiella tarda에 대한 효능

김영대,오명주,정성주,Kim, Young-Dae,Oh, Myung-Joo,Jung, Sung-Ju 한국해양바이오학회 2006 한국해양바이오학회지 Vol.1 No.3

어류의 세균성질병 원인체인 Edwardsiella tarda 감염의 예방과 치료에 E. tarda로 면역한 계란으로부터 얻어진 난황항체 (IgY)의 잠정적인 사용을 평가하였다. PEG법으로 정제된 IgY는 64 kDa의 heavy chain과 27 kDa의 light chain을 가지고 있었다. IgY는 사료 공급 후 위의 pH가 3.4로 낮아지는 2시간째에는 대부분의 활성을 상실하였으나, 장내에서는 IgY의 활성이 나타났다. IgY의 효능 실험 결과 감염 초기에 IgY를 20 mg/fish로 공급한 그룹은 모든 실험에서 대조 그룹에 비해 높은 생존율을 나타내었으며, 장기 내에서 균의 감염 정도 역시 낮았다. 그러나 감염 후기에서는 생존율에 있어서의 유의적인 차이를 확인하기 힘들었다. 이는 넙치의 위에서 대부분의 IgY가 활성을 잃어 낮은 농도만이 장으로 이동하기 때문일 것으로 사료되며, 이 농도에서 IgY는 E. tarda에 대한 뚜렷한 예방이나 치료의 효과 보다는 감염시기를 어느 정도 연장시키는 역할을 하는 것으로 추정된다. The present study evaluated effect of egg yolk immunoglobulin (IgY) from the hen immunized with Edwardsiella tarda. The purification of anti-E. tarda IgY was performed by polyethylene glycol (PEG). Purified IgY had heavy chain of 64 kDa and light chain of 27 kDa size. The IgY was instable against olive flounders digestive factors and artificially modulated pH 2 and 3. Nevertheless, some activity of IgY appeared in intestine. IgY was orally administered with viable E. tarda to the olive flounders and the efficacy of protection against E. tarda infection was evaluated. Orally administered IgY at a dose of 20 mg/fish delayed infection period of E. tarda cannulated at $10^{6{\sim}8}CFU/fish$ to small size (30~40 g) and middle size (110~120 g) flounder. Moreover, orally administered IgY at dose of 20 mg/fish inhibited the penetration of E. tarda cannulated at $10^8CFU/fish$ into the liver, kidney, spleen and gill via intestine. The fish orally administered with IgY showed increased survival rate. These results suggest that egg yolk containing anti-E. tarda IgY is effective in preventing edwardsiellosis.

넙치 유래 viral hemorrhagic septicemia virus (VHSV, genotype IVa)에 대한 단클론 항체 생산

정하나,장민석,오명주,김위식,Jeong, Ha-Na,Jang, Min-Seok,Oh, Myung-Joo,Kim, Wi-Sik 한국어병학회 2017 한국어병학회지 Vol.30 No.2

Mouse monoclonal antibodies (MAbs) were produced by using viral hemorrhagic septicemia virus (VHSV, genotype IVa) as an immunogen, isolated from diseased olive flounder (Paralichthys olivaceus). Four hybridoma clones secreting MAbs against VHSV were established. The MAbs were recognized the nucleoprotein (MAb 4), phosphoprotein (MAb 1) and matrix protein (MAbs 2 and 3) of VHSV by western blot analysis. Among them, the MAbs 1 and 4 strongly reacted with the VHSV-infected FHM cells, but not normal FHM cells. In enzyme linked immunosorbent assay, the four MAbs reacted with the VHSV, but not different six fish viruses (infectious hematopoietic necrosis virus, hirame rhabdovirus, spring viraemia of carp virus, infectious pancreatic necrosis virus, marine birnavirus and nervous necrosis virus). These results indicate that the MAbs are useful for diagnosis of VHSV infection.

국내 양식산 대하(Penaeus chinensis)에 있어서의 white spot syndrome virus (WSSV) 감염특성

김영진(Yeong Jin KIM),오명주(Myung Joo OH) 전남대학교 수산과학연구소 2004 수산과학연구소논문집 Vol.13 No.-

Since 1993, mass mortality has been caused by viral infection on aquaculturing shrimp, and gave to enormous losses to culturing fishery every year. The causative virus associated with white spot syndrome that showed the white spots on the inside of the carapace resulted in mass mortality to the Asian shrimp industry as well as Korea. This study was performed to reveal the infective features of the causative agent through internal and external observation on infected shrimps. In external signs, showed the symptom that red coloration of appendage, and discoloration of body as well as white spots. In histopathological observation, infected shrimp tissues were identified inclusion bodies as in other shrimp viruses, Baculovirus penaei (BP), baculoviral mid-gut gland necrosis virus (BMNV), Penaeus monodon-type baculovirus (MBV), etc. Also, in some case, the histopathological sign by hepatopancreatic parvovirus (HPV) was identified. Infected shrimp by WSSV has a hematological characteristics as follows. First, the number of shrimp hemocyte was slightly decreased as time goes by viral infection. Second, hemocyte composition in the infected shrimp showed that granular and semi-granular cell was decreased, however, hyaline cell increased. In view of the fact that granular cells play an important role in non-specific defense mechanism for foreign pathogen in shrimp.

임플란트 지대주 나사에 요구되는 물리적 성질

오동준 ( Dong Joon Oh ),한명주 ( Myung Joo Han ),정재헌 ( Chae Heon Chung ) 조선대학교 구강생물학연구소 2005 Oral Biology Research (Oral Biol Res) Vol.29 No.3

다시마(Saccharina japonica) 배우체의 미동정 진균증

정하나 ( Ha-na Jeong ),오명주 ( Myung-joo Oh ),최성제 ( Sung-je Choi ),서정수 ( Jung-soo Seo ),박명애 ( Myoung-ae Park ),김위식 ( Wi-sik Kim ) 한국수산과학회 2017 한국수산과학회지 Vol.50 No.2

In 2015, white cottony tufts were observed on gametophytes of the kelp Saccharina japonica. Wet mount and histopathology examination revealed numerous fungal hyphae and mycelium around the gametophytes. The gametophytes surrounded by fungal hyphae were generally round and empty. A specific 610-bp fragment of the internal transcribed spacer (ITS)-5.8S rDNA-ITS gene of fungi was amplified by polymerase chain reaction and the nucleotide sequence showed 100% identity with those of Acremonium sclerotigenum, Acremonium sp. and Ascomycota sp. When fungus-infected gametophytes were mixed with healthy gametophytes, a high transmission rate (100%) resulted. This is the first report of mycosis of gametophytes in Korea.

흰다리새우(Litopenaeus vannamei )에서 분리된 WSSV의 전장유전체 분석

이아름,공경희,김휘진,오명주,김도형,김종오,김위식,Lee, A-reum,Kong, Kyoung-Hui,Kim, Hwi-Jin,Oh, Myung-Joo,Kim, Do-Hyung,Kim, Jong-Oh,Kim, Wi-Sik 한국어병학회 2022 한국어병학회지 Vol.35 No.1

The full genome sequence of a Korean white spot syndrome virus (WSSV, isolate: WSSV-GoC18) is presented here. We obtained a total of 12,320,554 reads with 291,172 bases, 170 gene, and 170 coding DNA sequence, which were assembled in 1 contig. Phylogenetic analysis revealed that the WSSV-GoC18 was closely related to Chinese isolate (WSSV-PC) and distinctly different with previously reported a Korean isolate (WSSV K-LV1). The complete genome sequence of WSSV isolates will be of great help in molecular epidemiological studies, contributing to molecular diagnosis and disease prevention in shrimp aquaculture.

Infectious hematopoietic necrosis virus (IHNV)에 대한 단클론 항체 생산

공경희 ( Kyoung-hui Kong ),오명주 ( Myung-joo Oh ),김춘섭 ( Choon-sup Kim ),김위식 ( Wi-sik Kim ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2

Infectious hematopoietic necrosis virus (IHNV) is s significant viral pathogen affecting cultured rainbow trout (Oncorhynchus mykiss) in Korea. In this study, five monoclonal antibodies (mAbs) (IHNV-1, 2, 3, 4, and 5) were produced using purified IHNV. Reactivities of these mAbs were analyzed by western blot (WB), enzyme-linked immunosorbent assay (ELISA), and indirect fluorescent antibody test (IFAT). These mAbs recognized glycoprotein (69 kDa, IHNV-1), nucleocapsid protein (39 kDa, IHNV-3, 4, and 5), or phosphoprotein (27 kDa, IHNV-2) of IHNV by WB analysis. ELISA results indicated that these five mAbs were specific to IHNV without showing any cross-reactivity against other fish viruses (hirame rhabdovirus, infectious pancreatic necrosis virus, and viral hemorrhagic septicemia virus). IFAT demonstrated specific fluorescence signals of IHNV-infected epithelioma papulosum cyprini (EPC) cells, whereas no reactivity of normal EPC cells was observed. These mAbs can be very useful for immuno-diagnosis of IHNV infection.