유기용매에 의한 EcoRI 제한효소의 특이성 변화

엄재영,박충웅,이강민,Um, Jae-Young,Park, Chung-Ung,Lee, Kag-Min 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5

DNA를 인식하여 절단하는 제한효소의 발견은 실험실에서 유전자를 연구, 조작할 수 있게되어 분자생물학 연구에 큰 발전을 가져왔다. 제한효소의 인식자리는 반응용액의 산도, 이온세기, 소수성, 유기용매, 효소의 양에 따라서 달라질 수 있다. 본 연구는 유전공학에서 가장 많이 이용되고 있으며 그의 3차 구조가 밝혀진 EcoRI 제한효소가 유기용매에 의한 특이성 변화를 연구하였다. 이 효소의 특이성은 에탄올, ethyleneglycol, DMSO와 같은 유기용매에 의하여 변화되며, 이 변화는 유기용매의 소수성(LogP)값과 밀접히 관계있다. EcoRI의 유기용매에 의한 특이성변화는 LogP값이 -2.0~0 사이에서 일어난다. Acetone, 2-methyl-propanol같은 그 효소를 쉽게 비활성화시키는 유기용매는 특이성을 변화에 영향을 주지못한다. 이러한 특이성 변화는 무질서하게 일어나지 않고 순서적으로 일어난다. 10% DMSO에서 EcoRI을 이용하여 pGEM3를 절단할 때 다음 절단자리는 TAATTC, GAGTTC순서로 절단된다. 이와 같은 제한효소의 반응조건을 바꾸면 고유의 절단자리가 아닌 다른자리를 절단할 수 있으며 이러한 기술은 유전공학에 이용될 수 있다. In molecular biology, type-II restriction endonuclease. which specifically cleave DNA at a limited number of sites, have been exploited as a means of characterizing DNA fragments, DNA mapping and of modifying DNA for genetic engineering. Recently, many type-II restriction endonucleases have been found to decrease their substrate specificity under modified conditions such as extreme pH, low ionic strength, high enzyme concentration, substitution of metallic cofactors, or addition of organic solvents. This study used restriction endonuclease EcoRI which are used most frequently in genetic engineering. We investigated their specificity change in buffer condition including various organic solvents. The specificity of cleavage of EcoRI is altered in the presence of hydrophobic reagents, such as ethanol, ethyleneglycol and DMSO. The enzyme recognition site was not changed randomly but by preferential order by increasing the concentration of organic solvent. When EcoRI reacted with substrate pGEM3 vector which have one canonical recognition site (GAATTC), EcoRI cleaved noncanonical TAATTC and GAGTTC subsequently in more than 10% DMSO solution. These changes of specificities depended on the hydrophobicity of organic solvent (LogP: partition coefficient). As a results, the recognition sequence site was changed in the presence of organic solvents whose LogP are -2.0~0. The specificities were not easily changed in enzyme inactivating organic solvent such as acetone, 2-methyl-2-propanol, 2-methyl-1-propanol. These results might show that restriction enzyme could be used to cleave at unusual site by changing the reaction condition.

태권도 태극품새와 가라테 카타 평안의 기술체계 분석

엄재영(Um, Jae-Young),이재봉(Lee, Jae-Bong) 세계태권도문화학회 2016 세계태권도문화학회지 Vol.- No.13

This thesis is to compare Taekwondo s Teageuk Poomsae and Karate Kata Pingan to analyse relation between these two in order to show what are the similarities and differences. Therefore, new Poomsae can be designed to fulfill the needs of new era. Thesis uses purposeful sampling and gathers 3 Karate experts who holds more than 5th degree black belt in Taekwondo. They were to discuss between TaeKondo and Karate with more than five times, and brought up their opinions on both martial art. Also research on the thesis is based on not only new theory but also old Korean martial writing materials. The results are as below. First, Taegeuk Poomsae is form to fulfill the needs of Minster of Education to be used and a physical education program in middle and high school. However, it is also to be used as training program in Taekwondo Do-Jang to teach beginners and trainees. Kata Pingan is also created by Itos Yasutsune with same purpose in order to follow Japan s national P.E. program planning. In 1905, Okinawa 1st Middle School and Okinawa school of education made Karate as official program. Because of this, Pinan do excluded lethal technique such as attacks a vital point or turning over joints. Even though, Taegeuk and Pinan was made with similar purpose there is no evidence that one is used the other in its forming. Second, system of Taeguek and Pinan is similar. However, its technique is very different. Taeguek includes Dollyeochgi, pyojeokchagi, ieochagi Ttwieochagi, Dubaldangseongchagi and etc... Kata Pinan does not have above high level kick technique. Taekwondo has Bakkanmakgi, Dollyeochgi, and Apseogi which Karate Kata doesn t have. It means that Taekwondo and Karate have close system but not the techniques according to the research of this thesis. Third, kicking technique of Taekwondo has been traditional Korean style ever since Chosun period as it written in the book Haedongjukji and Taekwondo in modern history and the new controversy. Pinan has low kick and body kick. However, there is no head kick or any other high kicks in the Kata according to study of this thesis. Forth, Taekwondo took some of Karate Kata s system and structure. and rules. However, after Japanese colonial era, Taekwondo re-organized as Korean martial art and developed rapidly. Fifth, in 1960 s Taekwondo has better practical technique and it is proved by its competition style. Karate used Sundome. This means that an attacker stops his or her striking before the target. Taekwondo, on the other hand, made a rule of full powered real striking to targets and show its unique style of fighting to the world. Taekwondo is so powerful that people had to wear bamboo protecting gear and now Electronic protecting gear is developed. Later this became Hogu and electronic Hogu. Lastly, with the study of color belt level Poomsae, study on black belt Poomsae can be more expected next. To improve Poomsae in Taekwondo, new and better Poomsae must be made soon. The new Poomsae must include higher level kicking techniques to characterize Taekwondo s style stronger. Also there is no Pommsae-Pulee for Taegeuk, therefore, there should be more study on the matter.

수풍순기환 분할처방 투여가 고지방, 고탄수화물 식이로 유발된 비만형 제2형 당뇨병 동물모델에 미치는 영향

박은영,안세영,안영민,엄재영,장형진,이병철,Park, Eun-Young,Ahn, Se-Young,Ahn, Young-Min,Um, Jae-Young,Jang, Hyeung-Jin,Lee, Byung-Cheol 대한한방내과학회 2011 大韓韓方內科學會誌 Vol.32 No.3

Objectives : Recently a lot of research is being done for find antidiabetic medicine which has no side effects. This study aimed to investigate the antidiabetic and antiobesity effects of Supungsunki-hwan partitioned prescriptions on obese type 2 diabetes mouse. Methods : Type 2 diabetes mellitus and obesity were induced by Surwit's high fat, high sucrose diet for 8 weeks. Mice were divided into 3 groups of ND (normal diet, n=10) HFD (high fat and high sucrose diet, n=10) and SPP (high fat and high sucrose diet with Supungsunki-hwan partitioned prescriptions, n=10) groups. Body weights were measured every week. After 7 weeks, fasting blood sugar and oral glucose tolerance tests were conducted. After 8 weeks, blood samples of all mice were taken from their heart and analyzed biochemically. At the same time, epididymal fat pad and liver weights were measured. Histological size of white adipocyte were measured as well. Results : Compared with a HFD group, body weight, fructosamine, epididymal fat pad weight and white adipocyte size decreased. High-density lipoprotein cholesterol levels increased in the SPP group. Conclusions : These results suggest that SPP has antidiabetic and antiobesity effects in high fat, high sucrose diet induced obese mice.

搜風順氣丸加減方 투여가 비만형 제2형 당뇨병 동물모델의 당대사 및 지질대사에 미치는 영향

송상열(Sang-Yel Song),안세영(Se-Young Ahn),안영민(Young-Min Ahn),엄재영(Jae-Young Um),장형진(Hyeung-Jin Jang),이병철(Byung-Cheol Lee) 대한한의학회 2011 대한한의학회지 Vol.32 No.5

클로렐라의 항 피로 효과 연구

안효진 ( Hyo Jin An ),서상완 ( Sang Wan Seo ),심경식 ( Kyung Sik Sim ),김정숙 ( Jung Suk Kim ),김은희 ( Eun Hee Kim ),이미옥 ( Mi Ok Lee ),박형석 ( Hyeung Suk Park ),한재갑 ( Jae Gab Han ),이은희 ( Eun Hee Lee ),엄재영 ( Jae Young 한국식품영양학회 2006 韓國食品營養學會誌 Vol.19 No.2

난소암 조직에서 Mycoplasma DAN 존재의 의의 : PCR-ELISA 기법을 이용한 검출 Detection by PCR-ELISA Technique

이명희,남주현,김용만,김종혁,목정은,나준희,김영탁,엄재영 대한부인종양 콜포스코피학회 1998 Journal of Gynecologic Oncology Vol.9 No.1

Mycoplasmas, cell wall-less bacteria of class Mollicutes, are among the smallest self-replicating organisms known and reside ubiquitously at the cell membrane or internalized into the cell. They mimic viruses in many of their activities and further they may have oncogenic activity. The oncogenic potential of mycoplasmas was only recently realized when they were shown to cause chromosomal changes and in vitro cell transformations through gradual progressive chromosomal loss and translocations. The association between these organisms and human cancers has been evaluated and actually mycoplasmas were detected in 50% of gastric cancers. In gynecologic cancer, one study demonstrated a 59.3% prevalence rate of mycoplasmas in malignant ovarian tumors but the explanations for the association between the organisms and ovarian cancer might be somewhat confusing, at least in part, due to absence of normal control. The present objective was to determine the presence of mycoplasma DNA in ovarian cancer tissues and normal ovary in Korea. Fresh frozen tissue samples stored at -72 ℃were used for mycoplasma DNA assay. The study materials comprised twenty-nine human ovarian cancer tissues and ten normal ovarian tissues. After extraction of DNA, the combined PCR-ELISA(polymerase chain reaction and enzyme linked immunosorbent assay) procedure was performed with consensus primers targeting for 15 species of mycoplasmas and acholeplasmas together with negative and positive controls, which was known as very sensitive method. The results showed mycoplasma DNA were present in none of normal ovarian tissue and in 13.8%(4 of 29) of the ovarian cancer specimens, which is much lower than that of the previousstudy. Three positive cases showed very strong reactivities, but there was no significant correlation between presence of mycoplasma DNA and the clinicopathological characteristics of the patients. These results suggest that mycoplasma can not be the contributor in the mechanism of carcinogenesis in the most of ovarian cancers in Korea,ut the association between mycoplasma and ovarian cancer is worth to be investigated.