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      • SCIEKCI등재

        사과 겹무늬썩음병에 걸린 가지로부터 분산되는 병포자의 정량적 조사법 개발

        양희정,최창희,우현,김대희,엄재열,Yang, Hee-Jung,Choi, Chang-Hee,Woo, Hyun,Kim, Dai-Hee,Uhm, Jae-Youl 한국식물병리학회 1998 Plant Pathology Journal Vol.14 No.4

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        On the basis of the fact that the pycnidiospore of Botryosphaeria dothidea, the causal fungus of apple white rot is a typical water borne spore, a method for quantitative analysis of pycnidiospore dispersal from the warts produced on the diseased apple tree stem was developed. The warts on which cracks developed either on or around them were cut off at the base, and shaked in the water for 4hours at 2$0^{\circ}C$, in which condition the maximum number of spores were released. The volume of shaking solution was calculated as 1 ml per one wart. At the end of shaking, Trio, a household detergent was added to the shaking solution to the concentration of 0.1%, and shaked for additional 10 minutes at 35$^{\circ}C$ to take off the spores attached on the glass ware. One milliliter of the spore suspension thus prepared were passed through transparent membrane filter (pore size : 3.0 ${\mu}{\textrm}{m}$), and the spores attached on the filter were counted under a microscope ($\times$200) after staining them with lactophenol supplemented with aniline blue. The results thus obtained were statistically consistent when at least 30 warts were used simultaneously in single shaking. This method can be applicable in the elucidation of ecology of sporulation and spore dispersal, and also in the screening of the sporulation inhibitor which can be used in the control of the disease by reducing the inoculum density.

      • KCI등재

        High Yield Bacterial Expression and Purification of Active Cytochrome P450 p-coumarate-3-hydroxylase (C3H), the Arabidopsis Membrane Protein

        양희정,김완연,윤영주,윤지원,권태우,윤혜숙,윤부현,Yang, Hee-Jung,Kim, Wan-Yeon,Yun, Young-Ju,Yoon, Ji-Won,Kwon, Tae-Woo,Youn, Hye-Sook,Youn, Bu-Hyun Korean Society of Life Science 2009 생명과학회지 Vol.19 No.8

        다양한 천연물의 합성대사에 관여하는 식물 cytochrome P450 (P450s)은 그 기능적 다양성에도 불구하고, 이들 효소의 광범위한 기질 특이성을 설명해 줄 수 있는 구조분석에 대해서는 충분한 연구가 이루어지지 못하고 있는 실정이다. 식물 p-coumarate 3-hydroxylase (C3H)에 의해 매개되는 효소 반응은 lignin 과 다양한 phenylpropanoid 부산물들의 생합성에 매우 중요한 것으로 여겨지지만, 막 단백질인 C3H의 발현 및 정제가 효과적으로 이루어지지 못하여, 활성을 측정하기 위한 분석방법이 체계화 되지 못하고 있다. C3H의 작용기작과 기질특이성에 대해 폭넓은 이해를 위한 구조분석의 선행단계는 활성을 갖는 C3H를 밀리그램 단위로 분리, 정제하는 실험적 방법을 확립하는 것이라 할 수 있다. 이를 위해, 본 연구에서는 다양한 돌연변이 방법을 도입하여 식물 막단백질 C3H를 대장균 시스템에서 효과적으로 발현 및 정제할 수 있는 시스템을 사용하였다. 변형된 cytochrome P450 C3H ($C3H_{mod}$)을 세포막으로부터 고농도의 염완충용액을 이용하여 계면활성제 없이 추출하였으며, 2단계 chromatography를 통해 활성을 유지한 상태로 분리할 수 있었다. 이러한 실험적 기법은 NMR 및 X-ray crystallography와 같은 구조분석을 통한 C3H의 효과적인 분석에 적용될 수 있을 것이며, 또한 다른 식물 cytochrome P450 단백질의 효과적인 분석에도 적용 될 수 있을 것이다. The cytochrome P450s (P450s) metabolizing natural products are among the most versatile biological catalysts known in plants, but knowledge of the structural basis for their broad substrate specificity has been limited. The activity of p-coumarate 3-hydroxylase (C3H) is thought to be essential for the biosynthesis of lignin and many other phenylpropanoid pathway products in plants however, all attempts to express and purify the protein corresponding C3H gene have failed. As a result, no conditions suitable for the unambiguous assay of the enzyme are known. The detailed understanding of the mechanism and substrate-specificity of C3Hdemands a method for the production of active protein on the milligram scale. We have developed a bacterial expression and purification system for the plant C3H, which allows for the quick expression and purification of active wild-type C3H via introduction of combinational mutagenesis. The modified cytochrome P450 C3H ($C3H_{mod}$) could be purified in the absence of detergent using immobilized metal affinity chromatography and size exclusion chromatography following extraction from isolated membranes in a high salt buffer and catalytically activated. This method makes the use of isotopic labeling of C3H for NMRstudies and X-ray crystallography practical, and is also applicable to other plant cytochrome P450 proteins.

      • KCI등재

        장년 성인의 특성들을 고려한 웹기반 코스웨어 설계 방안

        양희정,이석균,Yang, Hee-Jung,Lee, Suk-Kyoon 한국정보처리학회 2003 정보처리학회논문지 A Vol.10 No.5

        As the use of computers and internet service has increased fast, various web-based coursewares for the educational purpose have been devoloped. However, the development of web-based coursewares for old-aged adults is far from satisfactory. Since most existing web-based coursewares are designed for the youth, they are not proper for old aged people to use confidently. In this paper, we analyse psychological, social and physical characteristics of old aged adults and propose the starategy for designing web based coursewares for them. Then, We apply the design strategy to develop the web-based courseware in Excel subject and analyse the effect of the design strategy by means of questioning and answring. 컴퓨터와 인터넷 사용의 급속한 확산에 따라 다양한 교육용 웹기반 코스웨어의 개발이 이루어지고 있다. 그러나, 장년 성인학습자를 위한 웹기반 코스웨어의 개발은 아직 미흡한 편이다. 기존의 웹기반 코스웨어는 주로 젊은 층을 대상으로 설계되어 있어 장년 성인들이 사용하기에 적당하지 않다. 본 논문에서는 장년의 성인들의 심리적, 사회적, 신체적 특성을 분석하고 이들을 위한 웹기반 코스웨어의 설계 방안을 제시한다. 그리고 제안한 설계 방안을 엑셀과목의 웹기반 코스웨어에 적용하고 그 결과를 설문조사를 통해 분석하였다.

      • KCI등재

        한국 에듀테인먼트 영상 콘텐츠의 아랍어 더빙 번역 연구: <뽀롱뽀롱 뽀로로>를 중심으로

        양희정 ( Yang Hee Jung ) 한국외국어대학교 중동연구소 2021 중동연구 Vol.40 No.1

        This study examines the Korean-Arabic translation strategy adopted to dub video content for the Korean edutainment animation series < Pororo the Little Penguin >. The use of Internet-based over-the-top(OTT) streaming platforms is increasing rapidly in the current age of distant communication resulted by the Covid-19 pandemic. Animations are spotlighted as educational content for the home, and < Pororo > has become extremely popular in both the domestic and foreign markets. This series has been operating an official YouTube channel since 2011 and offers content dubbed in 15 foreign languages, including Arabic. This study compared and analysed the titles and dialogues of the Korean, English and Arabic versions of < Pororo >. The results found that the animation series made use of relatively simple words and sentences to avoid complications in matching mouth movements. Additionally, the Korean original included numerous interjections, many of which were omitted in the Arabic version. The influence of English, the intermediate language, caused some of the lines to be omitted in the Arabic dubbing, which also intentionally excluded some episodes in consideration of the local culture and use of the content by the target audience comprising Muslim children.

      • KCI등재

        HLB 변화와 전상유화에 의해 형성된 에멀젼의 안정성

        양희정(Hee Jung Yang),김재현(Jae Hyun Kim),조완구(Wan Goo Cho),박수남(Soo Nam Park) 한국유화학회 2008 한국응용과학기술학회지 Vol.26 No.2

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        Caprylic/Capric triglyceride-in-water emulsions stabilized by Nikkol HCO-60 and HCO-10 were prepared using emulsion inversion point method at different HLB values. Emulsions with various droplet sizes were formed, and emulsion inversion point was detected by electrical conductivity. The change in emulsion droplet sizes and long term stability were monitored using laser scattering method and visual method. The droplet sizes and stability of emulsions were affected by HLB of surfactant. At emulsion inversion point, the water volume fraction increased as the HLB of surfactants decreased. According to our analysis, this resulted from a tendency of forming the W/O (water-in-oil) emulsion as the HLB of surfactants was decreased. The emulsion inversion point was clearly detected by the microscope and the electric conductivity meter. Nanometer-sized emulsion was obtained at the optimum HLB by using emulsion inversion point method. The main pattern of instability of emulsions in HLB 12 and 13 systems was Ostwald ripening. However, The patterns of instability of emulsions below 11 of HLB systems were Ostwald ripening and coalescence. All emulsions produced with surfactants in the range of HLB 8-13, creaming caused by density difference between water phase and oil phase.

      • KCI등재

        경한 난시를 보이는 환자의 백내장 수술 후 각막 후면 및 전체 난시의 변화

        양희정(Hee Jung Yang),박율리(Yu Li Park),김현승(Hyun Seung Kim) 대한안과학회 2015 대한안과학회지 Vol.56 No.11

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        목적: 각막 전면난시가 1.0D 미만인 환자에서 백내장 수술 후 각막 후면난시 및 전체 난시의 변화를 비교 분석하였다. 대상과 방법: 각막곡률이 가파른 축에 백내장 수술을 시행 받은 1.0D 미만의 각막 전면난시를 가진 환자 52안을 직난시를 보이는 33안(1군)과 도난시를 보이는 19안(2군)으로 분류하여 Pentacam으로 측정한 각막 전면, 후면, 전체 난시를 비교하였다. 결과: 직난시군에서 술 전 전면, 후면, 전체 난시는 0.55 ± 0.44D, 0.31 ± 0.14D, 0.30 ± 0.72D였으며 술 후 2개월 전면, 후면, 전체 난시는 0.51 ± 0.67D, 0.31 ± 0.15D, 0.35 ± 0.81D였다. 도난시군에서는 술 전 전면, 후면, 전체 난시는 -0.48 ± 0.46D, 0.26 ± 0.09D, -0.51 ± 0.65D였으며 술 후 2개월 전면, 후면, 전체 난시는 -0.17 ± 0.68D, 0.25 ± 0.13D, -0.30 ± 0.55D였다. 두 군에서 수술 전후 전면, 후면, 전체 난시에 유의한 차이가 없었다. 수술 2개월 후 수술 후 유발난시량의 vector analysis 시행 시 직난시군의 경우 전면난시의 surgically induced astigmatism (SIA)은 0.03D @ 30, 전체 난시의 SIA가 0.07D @ 74였으며 도난시군 의 경우 전면난시의 SIA는 0.27D @ 100, 전체 난시의 SIA가 0.36D @ 86로 측정되었다. 결론: 술 전 전체 각막난시가 1.0D 미만일 경우 후면난시가 수술 후 유발난시량의 예측 및 술 후 전체 난시에 미치는 영향은 적을 것으로 생각된다. Purpose: To analyze the change in posterior corneal astigmatism and total corneal astigmatism in patients with anterior corneal astigmatism less than 1.0 diopter (D). Methods: In the present study we evaluated 52 eyes with anterior corneal astigmatism less than 1.0 D. Patients were divided into 2 groups according to steep axis: Group 1 included 33 eyes with within-the-rule (WTR) astigmatism and Group 2 included 19 eyes with against-the-rule (ATR) astigmatism. Anterior, posterior and total corneal astigmatism were measured using Scheimpflug imaging (Pentacam). Results: In Group 1, preoperative anterior astigmatism, posterior astigmatism and total astigmatism were 0.55 ± 0.44 D, 0.31 ± 0.14 D and 0.30 ± 0.72 D, respectively. At postoperative 2 months, anterior astigmatism, posterior astigmatism and total astig-matism were 0.51 ± 0.67 D, 0.31 ± 0.15 D and 0.35 ± 0.81 D, respectively. There was no statistically significant difference be-tween preoperative and postoperative anterior, posterior and total corneal astigmatism in Group 1. In Group 2, preoperative an-terior astigmatism, posterior astigmatism and total astigmatism were -0.48 ± 0.46 D, 0.26 ± 0.09 D and -0.51 ± 0.65 D, respectively. At postoperative 2 months, anterior astigmatism, posterior astigmatism and total astigmatism were -0.17 ± 0.68 D, 0.25 ± 0.13 D and -0.30 ± 0.55 D, respectively. There was no statistically significant difference between preoperative and post-operative anterior, posterior and total corneal astigmatism in the 2 groups. There was no statistical correlation between pre-operative posterior corneal astigmatism and postoperative 2 months total corneal astigmatism. After vector analysis, surgically induced astigmatism (SIA) of the anterior and total astigmatism in Group 1 were 0.03 D @ 30° and 0.07 D @ 74°, respectively, and in Group 2 were 0.27 D @ 100° and 0.36 D @ 86°, respectively. Conclusions: In patients with preoperative total corneal astigmatism less than 1.0 D, posterior corneal astigmatism had a small effect on postoperative total corneal astigmatism.

      • 하수처리장의 방류수를 이용한 냉난방 시스템

        양희정(Hee-Jung Yang),백성권(Sung-Kwon Back),채규정(Kyu-Jung Chae),이동철(Dong-Chul Lee) 한국지반환경공학회 2012 한국지반환경공학회 학술발표회논문집 Vol.2012 No.9

        In this project aims at using thermal energy from the sewage system for heating and cooling buildings in the management office. The advantages of sewage is high value as a heat source. The temperature of the effluent of the wastewater treatment plant varies from 8 to 20 ℃ and thus contains thermal energy. When compared to air heat source is considered to be in good condition. In this study first analyzed the characteristics of the effluent characteristics located in Yong-in City, the last three years of data Giheung-Reseupiah. The sewage heat recovery system was designed using a heat pump as much as possible to reduce energy. We will analyze the cooling and heating performance of the experimental data. Finally, we are going to find the optimum operational conditions of economic and efficient.

      • KCI등재

        High Yield Bacterial Expression and Purification of Active Cytochrome P450 p-coumarate-3-hydroxylase (C3H), the Arabidopsis Membrane Protein

        Hee Jung Yang(양희정),Wanyeon Kim(김완연),Young Ju Yun(윤영주),Ji Won Yoon(윤지원),TaeWoo Kwon(권태우),HyeSook Youn(윤혜숙),BuHyun Youn(윤부현) 한국생명과학회 2009 생명과학회지 Vol.19 No.8

        다양한 천연물의 합성대사에 관여하는 식물 cytochrome P450 (P450s)은 그 기능적 다양성에도 불구하고, 이들 효소의 광범위한 기질 특이성을 설명해 줄 수 있는 구조분석에 대해서는 충분한 연구가 이루어지지 못하고 있는 실정이다. 식물 p-coumarate 3-hydroxylase (C3H)에 의해 매개되는 효소 반응은 lignin 과 다양한 phenylpropanoid 부산물들의 생합성에 매우 중요한 것으로 여겨지지만, 막 단백질인 C3H의 발현 및 정제가 효과적으로 이루어지지 못하여, 활성을 측정하기 위한 분석방법이 체계화 되지 못하고 있다. C3H의 작용기작과 기질특이성에 대해 폭넓은 이해를 위한 구조분석의 선행단계는 활성을 갖는 C3H를 밀리그램 단위로 분리, 정제하는 실험적 방법을 확립하는 것이라 할 수 있다. 이를 위해, 본 연구에서는 다양한 돌연변이 방법을 도입하여 식물 막단백질 C3H를 대장균 시스템에서 효과적으로 발현 및 정제할 수 있는 시스템을 사용하였다. 변형된 cytochrome P450 C3H (C3Hmod)을 세포막으로부터 고농도의 염완충용액을 이용하여 계면활성제 없이 추출하였으며, 2단계 chromatography를 통해 활성을 유지한 상태로 분리할 수 있었다. 이러한 실험적 기법은 NMR 및 X-ray crystallography와 같은 구조분석을 통한 C3H의 효과적인 분석에 적용될 수 있을 것이며, 또한 다른 식물 cytochrome P450 단백질의 효과적인 분석에도 적용 될 수 있을 것이다. The cytochrome P450s (P450s) metabolizing natural products are among the most versatile biological catalysts known in plants, but knowledge of the structural basis for their broad substrate specificity has been limited. The activity of p-coumarate 3-hydroxylase (C3H) is thought to be essential for the biosynthesis of lignin and many other phenylpropanoid pathway products in plants however, all attempts to express and purify the protein corresponding C3H gene have failed. As a result, no conditions suitable for the unambiguous assay of the enzyme are known. The detailed understanding of the mechanism and substrate-specificity of C3Hdemands a method for the production of active protein on the milligram scale. We have developed a bacterial expression and purification system for the plant C3H, which allows for the quick expression and purification of active wild-type C3H via introduction of combinational mutagenesis. The modified cytochrome P450 C3H (C3Hmod) could be purified in the absence of detergent using immobilized metal affinity chromatography and size exclusion chromatography following extraction from isolated membranes in a high salt buffer and catalytically activated. This method makes the use of isotopic labeling of C3H for NMRstudies and X-ray crystallography practical, and is also applicable to other plant cytochrome P450 proteins.

      • KCI등재
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