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녹용(鹿茸), 홍화자(紅花子) 단일 및 혼합 물 추출물( 抽出物)의 파골세포(破骨細胞) 분화(分化) 억제(抑制)와 골흡수(骨吸收) 억제(抑制) 효과(效果)
안지영,김주호,기지예,곽한복,오재민,김윤경,Ann, Ji-Young,Kim, Ju-Ho,Ki, Ji-Ye,Kwak, Han-Bok,Oh, Jae-Min,Kim, Yun-Kyung 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.2
Cervi parvum cornu (Deer Antler) and Carthami tinctorii fructus (Also known as Carthami seed) are widely used for treating osteoporosis and rheumatoid arthritis. In this study, We found out that the water extract of Cervi parvum cornu(WECPC), Carthami tinctorii fructus(WECTF) and their combination have effects of suppressing the RANKL-induced osteoclast differentiation. We assayed mRNA expression levels of NFATc1, c-Fos, TRAP and GAPDHS from bone marrow macrophages(BMMs) by means of RT-PCR. Similarly, the protein expression levels of NFATc1, c-Fos, MAPKs and $\beta$-actin in cell lysates were analyzed by means of Western blotting. then we determined the anti-osteoporotic effects of WECPC, WECTF and their combination using Lipopolysaccharide (LPS)-induced bone-loss mouse. WECPC, WECTF and their combination showed remarkable inhibition on RANKL-treated osteoclast differentiation without cytotoxicity. WECPC suppressed degradation of I-${\kappa}B$. WECPC, WECTF and their combination down-regulated the induction of c-Fos and NFATc1 by RANKL. Lastly, in vivo data showed that WECPC, WECTF and their combination rescued the bone erosion by LPS treatment. Thus, these results demonstrate that WECPC, WECTF and their combination can be efficacious remedies for bone-loss diseases such as osteoporosis and rheumatoid arthritis.
국내학술지에 발표된 한약추출물의 골다공증에 대한 효능실험 결과 고찰
안지영,기지예,김주호,박혜정,김현주,이정호,김윤경,Ann, Ji-Young,Ki, Ji-Ye,Kim, Ju-Ho,Park, Hye-Jung,Kim, Hyun-Ju,Lee, Jeong-Ho,Kim, Yun-Kyung 대한한의학방제학회 2008 大韓韓醫學方劑學會誌 Vol.16 No.2
Objectives : The purpose of this study is to inspect trends of the papers of osteoporosis in the field of herbal medicines and to establish the future direction for evaluation and development of natural medicines. Methods : We reviewed 80 oriental medicine papers in Korean journals published between 1994 and 2008. Results : There were 6 review, 2 clinical paper, 72 experimental papers and we confirmed that the drugs used in the experiments are almost yang-invigorating drugs which are good for kidney deficiency pattern Conclusions : We found out some of the herbal extracts are very efficacious for osteoporosis. More studies of oriental medicinal herbs are needed for developing anti-osteoporosis drugs with these basic data.
김은정,박혜정,김현주,김주호,안지영,이정호,김윤경,Kim, Eun-Jeong,Park, Hye-Jung,Kim, Hyun-Ju,Kim, Ju-Ho,Ann, Ji-Young,Lee, Jeong-Ho,Kim, Yun-Kyung 대한한의학방제학회 2008 大韓韓醫學方劑學會誌 Vol.16 No.1
Objectives : Hwangnyeonhaedok-tang(HNHDT) is the one of well-known traditional herbal formula and has been widely used for the treatment of various diseases in Korea. For the quality improvement of commercial traditional herbal formula, we investigated the quality of prepared HNHDT. Methods: We measured the contents of baicalin, berberine and geniposide in the HNHDT Ex preparations on the market by HPLC and compared with the official notice of ingredients. Results: In 3 preparations of HNHDT covered with the national health insurance(NHI), the content of baicalin was various from 32.83mg to 185.05mg, the total berberine was $12.0{\sim}50.75mg$ and geniposide was $12.57{\sim}44.84mg$. Contents of marker ingredients in NHI Preparations were below the standard and showed a large variation. In 7 non NHI Preparations of HNHDT and the standard(STD) HNHDT manufactured in laboratory, the content of baicalin was $37.16{\sim}115.69mg$, the total berberine was $5.62{\sim}28.43mg$ and geniposide was $10.98{\sim}113.47mg$. But, because composition of non-NHI preparations is different from NHI preparations, every maker's contents were up to the notice for baicalin and berberine. There was newly enforced standard regulations of geniposide in non-NHI preparations, so contents of geniposide was up to that standard only in JI and STD. Conclusions: There was a large variation of contents in the HNHDT Ex preparations on the market and the quality differences were existed between NHI and non-NHI preparations of HNHDT. Further studies for evaluation of efficacy differences and standardization of manufacturing processes are necessary.
김주호 ( Ju Ho Kim ),기지예 ( Ji Ye Ki ),안지영 ( Ji Young Ann ),박혜정 ( Hye Jung Park ),김현주 ( Hyun Ju Kim ),곽한복 ( Han Bok Kwak ),오재민 ( Jae Min Oh ),김윤경 ( Yun Kyung Kim ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.1
Objectives: Achyranthis Bidentatae Radix (ABR) has been used for treating of many symptoms especially osteoporosis and rheumatoid arthritis. In this study, we determined the effects of water extract of ABR in RANKL (Receptor Activator for Nuclear Factor κ B Ligand)-induced osteoclast differentiation culture system. Methods: We assayed mRNA expression levels of NFATc1, c-Fos, TRAP, OSCAR, FcRγ, DAP12 and GAPDH in bone marrow macrophages (BMMs) treated with ABR. The protein expression levels of NFATc1, c-Fos, MAPKs and β-actin in cell lysates treated with ABR were analysed by Western blotting. In addition we determined the effects of water extract of ABR on LPS-induced bone-loss mouse. Results: Water extract of ABR showed remarkable inhibition on RANKL-treated osteoclast differentiation without cytotoxicity. ABR down-regulated the induction of c-Fos and NFATc1 by RANKL. ABR suppressed phosphorylation of JNK, p38 and I-κB. ABR rescued bone erosion by LPS induction in vivo study. Conclusions: These results demonstrate that ABR may be a useful remedy for curing of bone-loss disease such as osteoporosis.