동결보존배를 이용한 일란성 다태생산에 관한 연구

신상태,Shin Sang-Tae 한국임상수의학회 1993 한국임상수의학회지 Vol.10 No.2

Although plenty of experiments for production of genetically identical twins have been reported, most of these reports were focused on microsurgical bisection of morula stage embryos, and little research has been performed for the production with frozen emb교os. The objective of these experiments were to assess the in vitro and in vitro developmental potential of blastomeres isolated from frozen-thawed 4-cell and 8-cell mouse embryos and to produce the identical multiplets from those embryos. The percentages of isolated 1/4, 2/4, 4/4, (control), 1/8, 2/8, 3/8, 4/8 and 8/8(control) blastomeres of 4-cell and 8-cell mouse emb교os which developed to normal blastocyst were 38.7%, 73.6%, 96.2(control), 15.1%, 40.1%, 65.9%, 88.3%, and 98.5%(control), respectively. The percentages of isolated 1/4, 2/4, 4/4(control), 1/8, 2/8, 3/8, 4/8 and 8/8(control) blastomeres of frozen-thawed 4-cell and 8-cell mouse embryos which developed to normal blastocyst were 35.6%, 68.5%, 100.0%(control), 16.1%, 50.6%, 71.7%, 90.9% and 100.0%(control), respectively. Normal 26 pups were obtained by transfer of 240 blastocyts developed 1, on 1/4 to 8/8 blastomeres. Those 26 pups obtained, 4 identical twins were produced from 2/4, 2/8, 3/8 and 4/8 blastomeres.

Selenium-Vitamin E 투여와 미량원소의 혈중수준이 젖소의 태반정체 발생에 미치는 영향

신상태,조충호,Shin, Sang-tae,Jo, Choong-ho 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.1

The effect of selenium and vitamin E on the incidence of retained fetal membranes(RFM) was evaluated in dairy cows raised in Kyonggi-do province from July through September 1985. Sodium selenite(0.1mg/kg of body weight) and vitamin E (1,000 IU) were simultaneously injected 21 days prior to the expected calving day to Holstein cows and the incidence in the treated group was compared with that in the non-treated control group. Serum levels of selenium, vitamin E, calcium, inorganic phosphorus and magnesium were also determined in the treated and the non-treated groups to compare the status of vitamin E and other minerals during periparturient period in the cows with RFM and the normal ones. The incidence of RFM was 34.5% in cows of the non-treated group (29 heads), whereas it was significantly reduced to 9.7% in cows of the treated group (31 heads) (p<0.05). Data for serum mineral concentrations showed that the prepartal inorganic phosphorus concentration was significantly lower in the RFM than in the not-retained group(p<0.01). As a result, the prepartal Ca/P ratio was significantly higher in the RFM group(p<0.01). It appears that a single injection of 0.1mg of sodium selenite per kg body weight and 1,000 IU of vitamin E 21 days prior to the expected calving day is an effective prophylactic for prevention of RFM, and that RFM may be related to imbalances in calcium and phosphorus metabolism.

동결보호제 및 Sucrose 농도가 급속동결한 마우스 집합배의 생존성에 미치는 영향

신상태,Shin, Sang-tae 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.4

The effects of ethylene glycol, DMSO and glycerol as cryoprotectants and the effect of concentrations(0, 0.25, 0.5 and 1.0M) of sucrose in the diluent on the viability of the aggregated morulae frozen rapidly in liquid nitrogen$(LN_2)$ vapour were examined. The morulae were produced by aggregation of ICR and CBA mice embryos at 8-cell stage. Before freezing the embryos were equilibrated in 1.5M cryoprotectants+0.25M sucrose in oae-step or in 3.0M cryoprotectants+0.25M sucrose in two-steps. The embryos were pipetted into the freezing medium fraction of 0.25ml plastic straws. The straws were frozeu by directly transfer into $LN_2$ vapour(about lcm above $LN_2$) for 2 minutes, and then plunged into $LN_2$. After thawing the cryoprotectants were diluted with 0, 0.25, 0.5 or 1.0M sucrose solution. The post-thawed in vitro viability of the aggregated embryos was significantly dependent on the type and concentration of cryoprotectants in the freezing medium and also on the concentration of sucrose in the diluent. When the aggregated embryos were equilibrated in 1.5M cryoprotectants +0.25M sucrose in one-step and diluted with 0.5M sucrose after thawing, the survival rate of the embryo5 was significantly(p<0.05) higher in DMSO(62.5%) or ethylene glycol(52.2%) than in glycerol(33.3 %). In the case that the concentration of the cryoprotectants was raised to 3.0M in two-steps, thc higher survival rate of the embryos was obtained in ethylene glycol or glycerol than in DMSO followed by diluting them with 0.5 or 1.0M sucrose after thawing(p<0.01).

사냥개에서의 Babesia gibsoni 감염

신상태,최희인,성재기,이창우,Shin Sang-Tae,Choi Hee-In,Sung Jai-Ki,Lee Chang-Woo 한국임상수의학회 1987 한국임상수의학회지 Vol.4 No.2

Babesia gibsoni infection was diagnosed and treated in three hunting dogs which were hospitalized to the Veterinary Teaching Hospital, College of Veterinary Medicine, Seoul National University between April 4 and August 29, 1987. All three dogs revealed severe anemia, hemoglobinuria, splenomegaly and markedly decreased PCV, RBC count and hemoglobin. The anemia was regenerative, as characterized by increased numbers of nucleated erythrocytes, polychromasia, anisocytosis, reticulocytosis. B. gibsoni was identified by examination of blood smear stained with Giemsa stain. The forms of B. gibsoni identified in this report were pleomorphic such as singnet ring, oval, comma, dot and elongated forms. The maximal percentages of erythrocytes infected with one or more B. gibsoni organisms were 39%, 20% and 40%, respectively. The Tick, Haemophysalis longicornis was assumed to be the vector of babesiosis in these cases. Specific treatment consisted of diminazene aceturate and supportive treatment consisted of whole blood transfusion, lactated Ringer's solution, vitamin B complex and broad spectrum antibiotics. All three dogs had convalesced successfully after treatment.

동결보존한 마우스 집합배의 생존성과 chimera의 생산에 관한 연구

신상태,조충호,Shin, Sang-tae,Jo, Choong-ho 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.2

The present study was carried out to investigate the viability of frozen-thawed aggregated mouse embryos and to produce the chimeras. Different phenotypic embryos were obtained by mating ICR female mice with ICR or CBA male mice. The aggregated morulae were produced following aggregation of embryos at 4-, 8- and 12- to 16-cell stage. The desirable stage for the aggregation of the mouse embryos was 8- to 16-cell stage. The post-thawed in vitro survival rates of aggregated embryos in glycerol, DMSO and ethylene glycerol were 51.5, 78.6 and 69.4%, respectively. Although the higher survival was obtained in DMSO, there were no significant differences in the survival rates among the three cryoprotectants. A total of 155 frozen-thawed aggregated embryos were transferred to 11 recipient mice, 3 out of 7 offsprings were born to overt chimera. These experiments have proven that mouse chimeras can be obtained from frozen-thawed aggregated embryos.

해사를 잔골재를 사용한 콘크리트의 촉진중성화에 관한 연구

신상태,유택동,최기봉,서치호,Shin, Sang-Tae,Yoo, Taek-Dong,Choi, Ki-Bong,Seo, Chee-Ho 한국구조물진단유지관리공학회 1999 한국구조물진단유지관리공학회 논문집 Vol.3 No.4

In this study, we executed fundamental experiment to investigate properties of accelerated carbonation with changing chloride content of concrete used sea sand in order to examine durability. So we obtained the results of following properties of mechanics, durability, concrete with sea sand, determined concrete w/C 30%, 40%, 50%, and fine aggregate 40% and changing containing chloride 0, 0.3, 0.6, $0.9kg/m^3$ by the experiment of accelerated neutralization. The results of this study as follows: 1) As result of changing chloride content of concrete used sea sand augmented in stages $0.3kg/m^3$, accelerated carbonation was increased as increment chloride content. The increment depth was decreased as it went long term age. It was shown the chloride content effected increment of carbonation depth in concrete 2) As a result of changing W/C of concrete used sea sand augmented in stages 10% at a time from 30% to 50%, accelerated carbonation depth of concrete was increased as W/C ratio. 3) As the carbonation concrete used sea sand, compressive strength between 8 weeks and accelerated carbonation depth of 1 weeks, 2 weeks, 4 weeks, 8 weeks was inversion proportion.

동결보존한 마우스 이분집합배의 생존에 관한 연구

신상태,조충호,Shin, Sang-tae,Jo, Choong-ho 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.2

Thc chimeric morulae were produced following aggregation of the half embryos which were microsurgically bisected at 8-cell and early morula stage. Different phenotypic embryos were obtained by mating ICR female mice with ICR or CBA male mice. The early morula stage was thc desirable stage for the aggregation of mouse embryos after bisection. The post-thawed survival rates of bisected-aggregated embryos that developed into normal blastocyst after conventional freezing in DMSO and ethylene glycol were 30.5 and 32.896, respectively. One offspring was produced by transferring the 67 frozen-thawed bisected-aggregated embryos.

간편한 Blowgun의 제작법

신상태,Shin Sang-Tae 한국임상수의학회 1987 한국임상수의학회지 Vol.4 No.1

A cheap, simple, and exact method for the preparation of handmade blowgun was studied. The blowgun-dart-adhesive -stand made of plastic syringe shaft was simple and convenient to make a blowgun dart accuratly. The needle end sealing method with wire-lead(solder) made the needle good to secure and simple to make. Because of easy exchangeability, the tail-piece made of yarn and a rivet is able to use permanently. And the materials of this blowgun were inexpensive, common and easily obtainable. This blowgun dart had various capacity up to 12ml.

사회안전망구축과 시민문화 및 지역사회결속에 대한 차이검증

신상태 ( Shin Sang-tae ) 한국안전문화연구원 2018 현대사회와 안전문화 Vol.1 No.1

연구목적: 본 연구는 사회안전망구축과 시민문화 및 지역사회결속에 대한 차이를 분석하는데 있다. 연구방법: 본 연구의 목적을 달성하기 위하여 2014년 7월 15일부터 9월 14일 까지 서울지역(강동, 강서, 강남, 강북) 일반 시민들을 모집단으로 선정한 다음 집락무선표집법(cluster random sampling)을 이용, 400명을 표집 하였다. 불성실한 자료를 제외하고 최종분석에 사용된 사례 수는 337명이다. 수집된 자료는 SPSSWIN 18.0을 이용하여 연구의 목적에 따라 분석하였으며, 통계기법은 요인분석, 신뢰도분석, 독립표본 t 검증, 일원변량분석 등의 방법이 활용되었다. 이 연구에서는 전문가회의, 예비검사, 요인분석, 신뢰도 분석 등을 통해 설문지의 타당도 및 신뢰도를 검증 하였다. 설문지의 신뢰도는 α=.727~α=.939으로 나타났다. 결론: 첫째, 사회인구학적 특성에 따라 사회안전망, 시민문화, 지역사회결속은 차이가 있다. 성별에 따른 지자체안전교육, 경찰치안서비스, 범죄예방설계는 남성 집단에서 높게 나타났다. 연령에 따라서는 60대 이상 집단에서 자율방범활동, 지자체안전교육, 경찰치안서비스가 높게 나타났다. 학력에 따른 자율방범활동, 지자체안전교육, 경찰치안서비스는 고졸이하 집단에서 높게 나타났다. 거주지에 따른 자율방범활동, 거리CCTV시설, 민간경비서비스는 강남 지역에서 높게 나타났으며, 지자체 안전교육은 강서 지역이 높게 인식하고 있음을 알 수 있다. 거주유형에 따라서는 아파트 집단에서 지자체안전교육, 민간경비서비스가 높게 나타났고, 자율방범활동은 단독주택집단에서 높게 나타났다. 거주기간에 따라서는 8~10년 집단에서 자율방범활동, 지자체안전교육, 경찰치안서비스가 높게 나타났다. 직업에 따른 경찰치안서비스는 자영업에서 높게 나타났고, 민간경비서비스는 가정주부 집단에서 그리고 자율방범활동, 지자체안전교육은 무직·기타 집단에서 높게 나타났다. 월수입에 따라서는 자율방범활동이 100만원미만 집단에서 높게 나타났고, 경찰치안서비스는 200~300만원 집단에서 그리고 범죄예방설계는 400만원 이상 집단에서 높게 나타났다. 시민문화에서 성별에 따른 참여의식은 남성이 높게 나타났다. 연령에 따른 참여의식에는 60대 이상이 높게 나타났다. 학력에 따라서는 고졸이하집단에서 참여의식이 높게 나타났다. 거주유형에 따라서는 아파트 집단에서 참여의식이 높게 나타났고, 단독주택 집단에서도 질서준법정신, 참여의식이 높게 나타났음을 알 수 있다. 거주기간에서는 8~10년 집단에서 참여의식이 높게 나타났다. 직업에 따라서는 가정주부 집단에서 참여의식이 높게 나타났다. 월수입에 따라서는 400만원 이상 집단에서 참여의식, 관용정신 모두 높게 나타났다. 지역사회결속에서 성별에 따른 안정감, 공동체의식, 지역사회제도는 남성 집단에서 높게 나타났다. 연령에 따라서는 50대 이상 집단에서 안정감, 공동체의식이 높게 나타났고, 60대 이상 집단에서 지역사회제도가 높게 나타났다. 학력에 따라서는 고졸이하 집단에서 공동체의식, 지역사회제도 모두 높게 나타났다. 거주지에 따라서는 강남지역에서 지역사회제도가 높게 나타났다. 거주유형에 따른 안정감, 지역사회제도는 아파트집단에서 높게 나타났다. 거주기간은 5~8년 집단에서 안정감이 높았다. 직업에 따른 지역사회제도는 무직·기타 집단이 높았다. 월수입에 따라서는 400만원 이상 집단에서 안정감이 높게 나타났다. Purpose: The purpose of this study is to analyze the difference between social safety net construction and civic culture and community solidarity. Methods: In order to accomplish the purpose of this study, general population of Seoul area (Gangdong, Gangseo, Gangnam, Kangbuk) was selected as a population from July 15 to September 14, 2014 and cluster random sampling was used. 400 people were sampled. Except for unfaithful data, the number of cases used in the final analysis is 337. The collected data were analyzed according to the purpose of the study using SPSSWIN 18.0. Statistical methods were factor analysis, reliability analysis, independent sample t - test, and one - way ANOVA. In this study, the validity and reliability of questionnaires were verified through expert meetings, preliminary tests, factor analysis, and reliability analysis. The reliability of the questionnaire was α = .727 ~ α = .939. Conclusion: The conclusion is as follows. First, the social safety net varies according to socio - demographic characteristics. The residence type is apartment group and single house, residence period is resident group of 8 ~ 10 years, occupation is self - employment and housewife group , was higher in the monthly income less than 1 million won group and 200 ~ 300 manwon group, more than 4 million won group. The difference according to citizen culture is male according to sex, age is 60, school is less than high school, residence type is apartment group and single house, 8 ~ 10 years group in residence period, family housewife group according to occupation, In the group of over 4 million won, participation consciousness, tolerance spirit is high. Differences in community cohesion gender male group, age was more than 50 groups and 60 or more groups, education was high school or less collective, Living in the Gangnam area, residence type apartments group, length of stay is 5-8 years group, Occupation was higher in unemployed and other groups, and monthly income was higher than 4 million won.

소 수정란이식(受精卵移植)에 있어서 수란우(受卵牛)와 수정란(受精卵)의 조건이 수태율(受胎率)에 미치는 영향

이정호,박항균,신상태,Lee, Jung-ho,Park, Hang-kyun,Shin, Sang-tae 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.1

This study was carried out to determine suitable selection factors for recipients and embryos which could improve pregnancy rates following bovine embryo transfer. The experiment included 52 surgical transfers from February, 1985 through June, 1986 performed on Kyungpook Breeding Center in southern Korea. The pregnancy rate was highest when recipients were in estrus within 6 hours before the donor to 12 hours after the donor (78.3% versus 50% for recipients in estrus earlier or later). Pregnancy rates were acceptable following culture under field conditions for up to 17 hours. More recipients over 15 months of age (76.1%) remained pregnant than those under 15 months (66.7%). Embryos transferred during the months from February to July resulted in higher pregnancy rates than those transferred during the remaining 6 months (77.3% versus 57.1%). Transferrable embryos were classified A (best) to C (worst); those graded A or B resulted in significantly higher pregnancy rates than those graded C (81.8% and 73.3% versus 25.0%, p<.05). Pregnancy rates among recipients of the Korean native breed tended to be higher than among Holstein recipients (100% versus 71.1%). Similarly, when the embryo was transferred to the right uterine horn, pregnancy rates tended to be higher than when it was transferred to the left (81.3% versus 65%). Pregnancy rates did not differ according to the stage of development of the embryo; they were for morulae, tight morulae, blastocysts, and advanced blastocysts, respectively: 75.0%, 66.7%, 75.0%, and 77.4%.