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냉동보관 제대혈 신생혈관 유도성세포배양과 허혈성 하지 쥐모델에서의 치료효과연구
김성환 ( Sung Whan Kim ),손영덕 ( Young Doug Sohn ),변기현 ( Ki Hyun Byun ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Currently, human bone marrow was thought to be the main source of stem cells. However, bone marrow derived stem cell is a limited source of cells for allograft transplantation, because it is not easy to collect bone marrow and they also have the possibility of viral infection. Therefore, as an alternative stem cell source, human umbilical cord blood(hUCB) could overcome the limitations of bone marrow derived stem cells. However, it has been difficult to isolate mesenchymal stem cells(MSCs) from cryopreserved hUCB due to the containment of rare populations of mesenchymal progenitor cells(MPCs). We tried to establish the culture system for angiogenic cells isolation from cryopreserved hUCB. As a result, we successfully could culture angiogenic cells that have endothelial specific surface markers and the capacity for differentiation potential into endothelial cells. In addition, transplantation of these cells into ischemic hindlimb mice showed that increased blood flow and higher limb salvage rate in the hindlimb ischemia. Immunohistochemistry results also demonstrated that hUCB derived cells are detected in the arterial walls of the ischemic hindlimb. Therefore, it is suggested that transplantation of cultured angiogenic cells derived from cryopreserved hUCB may be a novel and useful therapeutic armament for similar ischemic diseases.
효모 트레오닌 생합성 유전자의 subcloning 및 염색체 통합
김경훈,이호주,이우영,손영덕 한국유전학회 1995 Genes & Genomics Vol.17 No.1
The yeast gene THR4 encodes the threonine synthase (EC.4.2.99.2) which catalyses the last step of the threonine biosynthesis pathway. A recombinant plasmid pYL1(vector YCp50) has been cloned into E. coli HB101 from a yeast genomic library through its complementing activity of thr4 mutation in a yeast recipient strain M21-59. When subcloned into pYL12(vector YCp50) and pYL121(vector YIpS), the ClaI-ClaI fragment (4.4 kbp) of pYL1 insert was positive in threonine complementing activity in M21-59. The KpnI linearized pYL121 was introduced into the original recipient yeast strain and mitotically stable chromosomal integrants were identified among transformants. Using the genetic method of the tetrad analysis, the integration site of pYL121 was localized to the region of THR4 structural gene at the expected right arm of yeast chromosome III. It was therefore genetically proved that the cloned ClaI fragment contains the yeast THR4 structural gene of the genomic origin.