참깨 主要 特性의 遺傳

Nak Sul Seong(成洛戌),Jung Il Lee(李正日),Sang Il Park(朴相一) 한국육종학회 1987 한국육종학회지 Vol.19 No.4

Present study was conducted to investigate the inheritance of major agronomic characteristics in sesame with 6×6 diallel analysis. Over dominance was recognized in number of branches, number of grains per capsule and protein content, while plant height and 1,000 grain weight showed partial dominance. It was also recognized that dominant genes were prevalnt in the cultivars of Danbaekggae and Kansas-10 for plant height, in Ashrimutant for number of branches, in Kodal and Kansas-10 for number of grains per capsule, and in ashrimutant for 1,000 grain weight. Narrow sense heritabilities were relatively high with the range of 39-53% in plant height, number of branches and protein content, but number of grains per capsule and 1,000 grain weight showed lower heritablities.

참깨品種의 組合能力

Nak Sul Seong(成洛戌),Jung Il Lee(李正日),Sang Il Park(朴相一) 한국육종학회 1987 한국육종학회지 Vol.19 No.3

This study was conducted to investigate the combining ability and heterosis of sesame. 6x6 parental diallel analysis were attempted in 1986. Both GCA and SCA for all characteristics except palmitic acid content were significant. It was appeared that additive gene actions were more important for yield components, while non-additive gene actions were more important for palmitic acid, oleic acid and linoleic acid contents. ‘Danbaekggae’ was the best conbiner on yield related characteristics such as plant height, number of capsules per plant and grain weight per plant. Expression of heterosis was significant on yield related characteristics such as plant height, number of branchs, number of capules per plant and 1,000 grains weight per plant. Above results evidently indicated the possible use of Fi hybrid in sesame breeding.

토성에 따른 시호의 생육 및 Saikosaponin 함량

성낙술(Nak Sul Seong),김관수(Kwan Su Kim),소은희(Eun Hee Soh),채영암(Young Am Chae) 한국약용작물학회 1994 한국약용작물학회지 Vol.2 No.3

Bupleurum falcatum was cultivated in plots of different soil textures, which were sandy loam(SL), loam(L), and clay loam(CL). The growth characters of shoot and root parts in SL and L plots were better than CL one, and root yield was the most excellent in SL one. But the contents of total saikosaponins and methanol extract in its roots were the highest in CL plot. In any soil textures, one and two year old plants showed almost the same results in growth characters and saikosaponins contents. The growth and root yield of two year old plants were higher than those of one year old ones, but the contents of methanol extract and saikosaponins were lower. Plant characters were highly negative-correlated with the contents of saikosaponins and methanol extract.

산수유(山茱萸)(Cornus officinalisis)의 기내증식(器內增殖)에 관한 연구(硏究) I. 액아배양(腋芽培養)에 의한 Callus 유기(誘起), Shoot 증식(增殖) 및 뿌리 분화(分化)

박충헌,성낙술,이승택,윤규복,손서규,Park, Chung-Heon,Seong, Nak-Sul,Lee, Seung-Tack,Youn, Kyu-Bok,Son, Su-Gyu 한국약용작물학회 1993 韓國藥用作物學會誌 Vol.1 No.1

약용으로 널리 이용되는 산수유(Cornus officinalisis)의 기내육종을 위한 기초 연구로 액아배양을 통한 기내 대량증식 가성을 검토한 결과, 1) 액아 배양에 적합한 배지로는 MS 배지와 DKW 배지가 77%의 신초형성율을 보여 가장 양호하였고 WPM과 GD배지는 다소 저조하였다. 2) 얻어진 기내 Shoot의 증식에는 DKW가 6.8개의 신초를 형성하고 신초장이 4.5cm, 엽수도 10.8개로 가장 우수하였고 B5 > GD > MS >WPM의 순이었다. 3) Shoot 증식에 적합한 sucrose의 농도는 MS배지의 경우 3%가 좋았으며, 농도가 높아질수록 신초의 기내 생육에 제한적이었으며, 활성탄의 영향으로는 배지내 농도가 $2\;g\;/\;{\ell}$ 까지 증가할 수록 개체의 생육이 양호한 경향이었다. 4) MS배지의 경우 무기염류의 농도는 2MS에 비하여 1/4MS까지 낮아질수록 기내 생육에 적합하였다. 5) Shoot증식을 위하여 BA $0.5mg\;/\;{\ell}$에 auxin을 종류별로 혼합 첨가한 결과 2,4-D 첨가구의 경우 Callus의 생장이 가장 왕성하였으나 Shoot 증식에는 극히 제한적이었고, IAA와 IBA 처리는 Callus가 전혀 유기되지 않는 반면 multiple shoot가 형성되었으며, NAA에서는 Callus형성과 Shoot증식 이 모두 이루어졌다. 6) 신초의 기내 발근유도 시험에서는 MS매지에 NAA $0.5mg\;/\;{\ell}\;와\;BA\;0.5mg\;/\;{\ell}$를 첨가한 처리에서 신초로부터 정상근이 분화 되었으나 5% 비율로 낮았다. Present experiment were attempted to examine in vitro multiplication throughbud culture of Cornus officinalis. Bud derived shoot formation was established successfully on Murashige and Skoog's medium supplemented with $0.5mg\;/\;{\ell}$ BAP(N-benzyl amino purine). The shoot proliferation increased on the Driver Kuniyuki Walnut medium containing $0.5mg\;/\;{\ell}$ NAA(Napthalene acetic acid) and $0.5mg\;/\;{\ell}$ BAP. Addition of 2,4-D(2,4-Dichlorophenoxy acetic acid) to the media produced excessive callus inducton. IAA(Indole-3-acetic acid) and IBA (Indole-3-bu-tyric acid) enhanced multple shooting, and NAA showed callus induction and multiple shooting. Shoot growth was enhanced supplemented with 3% sucrose, $2g\;/\;{\ell}$ activated charcoal, and 1 / 4MS in organic salts. However, root formation of proliferated shoots was low about 5%

ACE 저해활성 측정

이승은,송진,성낙술,Lee Seung-Eun,Song Jin,Seong Nak-Sul 한국작물학회 2004 Korean journal of crop science Vol.49 No.suppl1

Genetic diversity and population structure of Atractylodes japonica $K_{OIDZ}.$ in Korea

허만규,성정숙,박춘근,박희운,성낙술,문성기,허홍욱,Huh, Man-Kyu,Sung, Jung-Sook,Park, Chun-Geon,Park, Hee-Woon,Seong, Nak-Sul,Moon, Sung-Gi,Huh, Hong-Wook The Korean Society of Medicinal Crop Science 2002 韓國藥用作物學會誌 Vol.10 No.1

한국내 분포하는 다년생 초본인 삽주 집단의 유전적 다양도와 집단구조를 조사하기 위해 전분 전기영동으로 분석하였다. 15 대립유전자좌위당 9개 좌위에서 다형현상(60.0%)을 보였으며, 유전적 다양도는 종수준에서 0.144로 높은 반면 집단수준이 이보다 약간 낮았다. 삽주의 유전적 다양도는 대부분 집단내에 존재하였고 유사한 생활양식을 가진 다른 식물종에 비해 높았다. 그 이유로는 유성생식, 다년생, 다산 등에 기인한다. 집단간 분화는 약 13%였고 지리적 거리와 유전적 거리의 상관은 높았다(r=0.65). 그럼에도 불구하고 일부 격리된 집단은 유효집단크기를 가지지 못하여 이형접합체의 결여가 유의성을 보여 다양도가 높은 집단의 보존이 요망된다. The study of genetic diversity was carried out in Atractylodes japonica $K_{OIDZ}$. Although this species has been regarded as medically important one, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of eight Korean populations of this species. Of the 15 genetic loci surveyed, nine (60.0%) was polymorphic in at least one population. Genetic diversity was high at the species level $(H_{es}=0.144)$, whereas, that of the population level was relatively low $(H_{ep}=0.128)$. Nearly 87% of the total genetic diversity in A. japonica was apportioned within populations. The sexual reproduction, high fecundity, and perennials are proposed as possible factors contributing to high genetic diversity. The indirect estimated of gene flow based on Gst was 1.69.

참취 분말이 에탄올을 투여한 흰쥐의 항산화계에 미치는 효과

이승은(Seung Eun Lee),성낙술(Nak Sul Seong),정태영(Tae Yung Chung),최미연(Mie Youn Choi),윤은경(Eun Kyong Yun),정유진(Yu Jin Jeung) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.6

In vitro 항산화 활성이 확인된 참취가 생체 내에서도 활성을 나타내는 지를 확인하기 위해 대조군과 5% 및 10% 참취분말을 첨가한 식이를 4주간 흰쥐에 투여하면서 마지막 1주간 알콜로 산화적 스트레스를 유발한 후 희생시킨 흰쥐의 체중 증가량과 함께 체중에 대한 장기의 상대 중량, 간, 신장에 서의 지질과산화, 항산화효소 활성, glutathione 및 혈청 알부민의 함량을 분석하였다. 4주간의 사육 후 체중 증가량은 대조군, 5% 참취첨가군 그리고 10% 참취첨가군의 순으로 높았으나 유의적인 차이는 없었으며 체중에 대한 상대적인 장기 중량 중에서 간, 비장은 대조군에 비해 참취 첨가군의 경우 더 낮은 비율을 나타내었다. 간에서 지질과산화로 생성되는 TBARS 함량은 대조군(66 μg)에 비해 5%(46 μg) 및 10% 참취 분말 식이군(35 μg)에서 참취첨가량이 증가할수록 낮았다. Mn-SOD와 catalase 활성은 참취 첨가군들에서 대조군보다 낮았으나 Cu, Zn-SOD와 glutathione peroxidase 활성 및 glutathione 함량은 실험군들간에 유의적인 차이를 나타내지 않았다. 신장에서는 Mn-SOD와 glutathione peroxidase 활성이 대조군보다 참취 첨가군에서 유사하거나 낮은 경향을 나타내었으나 Cu, Zn-SOD와 catalase의 활성 및 TBARS, glutathione의 함량은 실험군들간에 유의적인 차이를 보이지 않았다. 한편, 혈청 알부민의 함량은 실험군들간에 큰 차이가 없었다. 이상과 같은 실험결과를 종합할 때 참취는 에탄올에 의해 유발된 산화적인 스트레스를 적절하게 방어하여 특히 간장에서의 지질과산화 및 Mn-SOD 활성의 감소를 가져왔으며 그 효과는 참취의 첨가량에 비례하는 경향을 나타내었으므로 생체 안에서 알코올과 같은 산화적 스트레스에 대한 항산화제로서의 역할을 효과적으로 수행할 수 있을 것으로 기대된다. The present study was conducted to investigate effect of powdered herb of Aster scaber Thunb. (chamchwi) on antioxidant system in ethanol-administrated rats. Four week-old Sprague Dawley male rats which had initial body weights of 97.10±4.50 g were randomly divided into three groups: control (ethanol treated, vitamin E-deficient group); 5% chamchwi (ethanol-treated, 5% chamchwi powder-supplemented group): 10% chamchwi (ethanol-treated, 10% chamchwi powder-supplemented group). Three groups of rats were supplemented with three experimental diets for 4 weeks and orally administrated 10% ethanol (v/v) daily via drinking water in the last experimental week. Contents of TBARS (thiobarbituric acid reactive substance), glutathione in liver and kidney and serum albumin were determined. The activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-px) in liver and kidney were also analyzed. Relative weight of liver and spleen to body in chamchwi groups was lower than that in control group (p<0.05). The most remarkable result was that liver TBARS contents in chamchwi groups (5% chamchwi group, 46 μg in MDA value; 10% chamchwi group, 35μg) were significantly lower (p<0.05) than that in control group (66 μg). The supplement of chamchwi powder lowered the activity of manganese-superoxide dismutase (Mn-SOD), catalase in liver and GSH-px in kidney. The levels of glutathione in liver and kidney and serum albumin were not significantly different in all experimental groups (p<0.05). These results indicate that powdered herb of Aster scaber decreases lipid peroxidation and acitvity of Mn-SOD increased by alcohol-induced oxidative stress in liver of rats.

좌금환(左金丸)과 수련환(茱連丸)이 고지혈증에 미치는 영향

김이현 ( Yi Heon Kim ),성낙술 ( Nak Sul Seong ),이영종 ( Young Jong Lee ) 대한본초학회 2005 大韓本草學會誌 Vol.20 No.2

Multicolor FISH와 Feulgen 염색법을 이용한 Angelica속 식물의 세포유전학적 분석

구달회,김수영,방경환,성낙술,방재욱,Koo, Dal-Hoe,Kim, Soo-Young,Bang, Kyong-Hwan,Seong, Nak-Sul,Bang, Jae-Wook 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2

Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using multi-color fluorescence in situ hybridization (McFISH) technique were carried out in two Angelica species. The numbers of diploid chromosomes were the same in two same in two species as 2n=22, however the lengths of chromosomes were varied from 4.25 to 6.50 ${\mu}{\textrm}{m}$ in A gigas and 4.95 to 8.50 ${\mu}{\textrm}{m}$ in A acutiloba. The chromosomes of A. gigas were composed of five metacentric and six submetacentric pairs, while those of A. acutiloba were six metacentic, one submetacentric and four subtelocentric paris. In FISH experiments, the numbers and size of 45S rDNA signals were varied between two species, however dach signal of the 5S rDNA was observed in two species.

갯기름나물의 근과 경엽의 면역 및 항암 활성 비교 연구

조용호 ( Yong Ho Cho ),성낙술 ( Nak Sul Seong ),함인혜 ( In Hye Ham ),최호영 ( Ho Young Choi ) 대한본초학회 2004 大韓本草學會誌 Vol.19 No.3