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        • 흰쥐에서 발암물질로 유발된 갑상선 종양과 p21 및 p53 단백질의 발현

          백종민(Jong Min Baek),장석균(Suk Kyun Chang) 대한두경부종양학회 1999 대한두경부 종양학회지 Vol.15 No.2

          '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

          Objectives: The development of thyroid tumor has a relationship with carcinogen, oncogene and tumor suppressor gene. With aminotriazole, radioactive iodine and nitrosomethylurea as carcinogens in rat, authors investigate the incidence in type of the thyroid tumors, p21 and p53 protein expression pattern by immunohistochemical stain and the relationship between the tumors and p21-p53 protein expressions. Materials and Methods: 80 experimental animals were divided into four groups; group 1(control, no carcinogen, n=20), group 2(oral administration of aminotriazole for 36 weeks, n=20), group 3(intraperitoneal injection of 131I for one time and oral administration of aminotriazole for 36 weeks, n=20), group 4(oral administration of nitrosomethylurea for 3 days and aminotriazole for 36 weeks, n=20). After 40 weeks they were sacrificed with pathologic examination and we performed immunohistochemical staining with pan-ras monoclonal antibody for p21 protein and CMI polyclonal antibody for p53 protein with paraffin-embedded specimens. Results: 1) No tumors were observed in group I, but 38.3% of nodular goiters, 11.7% of adenomas and 50.0% of carcinomas were observed in carcinogen treated groups(group 2, 3, 4). 2) The incidence of nodular goiter, adenoma and carcinoma were 70%, 20% and 10% in group 2, 40%, 15% and 45% in group 3 and 5%, 0% and 95% in group 4. 3) p21 protein was not expressed in normal thyroid tissues but was expressed in 26.1% of nodular goiters, 42.9% of adenomas and 6.7% of carcinomas. On the other hands, p53 protein was not expressed in normal thyroid tissues, nodular goiters, adenomas and in well differentiated thyroid carcinomas by immunohistochemical stain. Conclusion: The authors suggest that aminotrizole, 131I, nitrosomethylurea can be etiologic agents in the development of thyroid tumor and the p21 protein can be expressed in the early stage and in benign condition of thyroid tumor but p53 protein is not expressed in all conditions of development in rats.

        • KCI등재

          파골세포의 분화와 뼈 흡수에 미치는 칡(Pueraria lobata)의 영향

          백종민(Jong Min Baek),윤강휴(Kang Hugh Yoon),안성준(Sung-Jun Ahn),박선향(Sun-Hyang Park),천윤희(Yoon-Hee Cheon),김주영(Ju-Young Kim),오재민(Jaemin Oh) 대한체질인류학회 2014 대한체질인류학회지 Vol.27 No.4

          칡은 뼈소실 모델인 난소를 적출한 마우스와 랫드에서 뼈형성을 촉진하다고 밝혀진 바 있다. 그러나 뼈파괴세포의 분화와 뼈흡수능에 미치는 칡의 효과와 기전은 아직 밝혀지지 않았다. 그러므로 본 연구에서는 칡이 recetor activator of nuclear factor-κB ligand (RANKL)과 macrophage colony stimulating factor (M-CSF)로 유도한 뼈파괴세포의 분화에 미치는 효과와 그 기전을 연구하였다. 우선적으로 뼈파괴세포 분화에 억제효과를 확인하기 위하여 마우스에서 분리한 큰포식세포에 칡을 농도별로 처리하여 tartrate-resistant acid phosphatase (TRAP) staining을 시행하였다. 또한 칡이 작용하는 기전을 알아보기 위하여 western blot 분석과 RT-PCR을 시행하였고, 성숙한 뼈파괴세포의 뼈흡수능에 미치는 영향을 알아보고자 hydroxyapatite 플레이트로 실험을 진행하였다. 그 결과, 칡은 뼈파괴세포 분화단계의 필수적인 초기 신호전달경로인 p38, Akt, c-Jun N-terminal kinase (JNK), IκB의 인산화를 억제하였다. 또한 nuclear factor of activated T cells (NFAT)c1과 c-Fos의 비활성화를 유도함으로써 뼈파괴세포와 관련된 유전자인 osteoclast-associated receptor (OSCAR), TRAP, Integrin β3, osteoclast stimulatory transmembrane protein (OC-STAMP), dendritic cell-specific transmem-brane protein (DC-STAMP)의 발현을 저해하였다. 특히, hydroxyapatite로 코팅된 플레이트를 통해서 뼈흡수능을 억제하는 칡의 효과를 확인하였으며, 이와 깊은 연관성을 가지는 유전자인 Cathepsin K의 발현 또한 저해하였다. 이러한 결과를 통하여 뼈파괴세포 분화와 뼈흡수능을 저해하는 칡의 효과와 이와 관련된 분자적 기전에 대하여 규명하였다. Previous researches have proved that Pueraria lobata up-regulates bone mineral contents and bone mineral density in bone-loss model, ovariectomized mice and orchidectomized rats. However, the precise effects and mechanisms of Pueraria lobata on osteoclast differentiation and bone resorbing activity of mature osteoclasts still remains unknown. Therefore, we investigated the effect and mechanism of Pueraria lobata on receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony stimulation factor (M-CSF)-induced osteoclast differentiation in bone marrow macro-phages (BMMs). First of all, we treated BMMs derived from mice with various concentrations of Pueraria lobata in order to perform screening by tartrate-resistant acid phosphatase (TRAP) staining. Also, we conducted western blotting and RT-PCR for the purpose of verifying the treatment mechanism of Pueraria lobata and lastly, we used hydroxyapatite-coated plate to evaluate the effects of Pueraria lobata on bone resorbing activity of mature osteoclasts. As a result, Pueraria lobata has inhibitory effect on phosphorylation of p38, Akt, c-Jun N-terminal kinase (JNK), and IκB which are essential early signaling pathway of osteoclastogenesis. Also, the inactivation of nuclear factor of activated T cells (NFAT)c1, and c-Fos which is caused by Pueraria lobata is followed by the suppression effects of Pueraria lobata on osteoclastrelated various genes, osteoclast-associated receptor (OSCAR), TRAP, Integrin β3, osteoclast stimulatory transmembrane protein (OC-STAMP), and dendritic cell-specific transmembrane protein (DC-STAMP). Particularly, Pueraria lobata blocks the formation of pit area on hydroxyapatite-coated plate in a dose-dependent manner as well as the mRNA expression of Cathepsin K, which is associated with bone resorbing activity. These results demonstrate the molecular mechanism relating to anti-osteoclastogenesis effect of Pueraria lobata as well as the inhibitory effect of Pueraria lobata on mature osteoclast formation and bone resorbing activity.

        • KCI등재

          상기생 추출물이 파골세포 분화와 골흡수 억제에 미치는 효과

          백종민(Jong Min Baek),김주영(Ju Young Kim),이명수(Myeung Su Lee),정우진(Woo Jin Jeung),문서영(Seo Young Moon),전병훈(Byung Hoon Jeon),오재민(Jae Min Oh),최민규(Min Kyu Choi) 한의병리학회 2013 동의생리병리학회지 Vol.27 No.4

          Bone homeostasis is maintained by co-ordination of bone-resorbing osteoclasts and bone-forming osteoblasts. Imbalance between osteoclasts and osteoblasts leads to many bone diseases such as osteoporosis, rheumatoid arthritis. Taxillus chinensis is a herb that has been widely used to improve bone health. However, the effect and mechanism of Taxillus chinensis extract on osteoclast differentiation and bone resportion has been unknown. Thus, We investigated the effect of Taxillus chinensis on expression of receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclast differentiation and bone resorption. Also, the action of Taxillus chinensis on mechanisms relating to osteoclast differentiation was studied. In this results, we identified that Taxillus chinensis significantly inhibited RANKL-induced osteoclast differentiation and bone resportion. Moreover, Taxillus chinensis was suppressed the activation of NF-kB in bone marrow macrophage treated RANKL and M-CSF. Taxillus chinensis was down-regulated the mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP). The cell adhesion-related molecules such as integrin av and integrin b3, and the filamentous actin (F-actin) rings of mature osteoclasts-related molecules such as dendritic cell-specific transmembrane preotein (DC-STAMP) and cathepsin K are also suppressed. Taken together, these results indicated that Taxillus chinensis will be a good candidate to treat osteoclast-mediated bone diseases.

        • KCI등재

          천속단(Phlomis umbrosa Turcz)의 파골세포 분화에 미치는 영향

          백종민(Jong Min Baek),박주하(Ju Ha Park),송정훈(Jeong Hoon Song),박성남(Seong-Nam Park),정우진(Woo Jin Jeong),문서영(Seo Young Moon),이창훈(Chang Hoon Lee),최민규(Min-Kyu Choi),김정중(Jeong Joong Kim),오재민(Jaemin Oh),장성조(Sung Jo 대한체질인류학회 2013 대한체질인류학회지 Vol.26 No.3

          골다공증, 류마티스 관절염을 비롯한 많은 골질환은 파골세포의 지나친 골 흡수 활동으로 인하여 발생한다. 최근 들어, 천연물에서 추출한 성분을 이용하여 새로운 치료물질을 개발하기 위한 연구가 활발하게 진행되고 있다. 그 중, 천속단이라고 알려진 천연물질은 뼈를 튼튼하게 해주는 효능이 있는 것으로 알려져 있으나, 천속단의 골질환과 관련된 정확한 효과나 치료기전에 대한 정보는 아직 알려진 바가 없다. 따라서 본 연구에서는 골수탐식세포에서 receptor activator of nuclear factor-κB ligand (RANKL)로 유도한 파골세포의 분화와 골 흡수능에 천속단이 어떠한 영향을 미치는지 알아보았다. 또한, 파골세포의 분화와 관련된 작용기전을 규명하였다. 본 실험에서 천속단은 RANKL로 유도한 파골세포의 분화와 골 흡수능을 현저히 억제하였고, RANKL과 macrophage-colony stimulating factor (M-CSF)를 처리한 골수탐식세포에서 NF-κB의 발현을 저해하였다. 더 나아가, c-Fos를 비롯한 nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP)의 mRNA 발현이 천속단에 의해 억제됨을 확인하였으며, 세포융합과 관련 있는 유전자인 Integrin αν, β3와 actin ring에 관여하는 유전자인 dendritic cell-specific transmembrane preotein (DC-STAMP)그리고 골 흡수능과 연관된 Cathepsin K 또한 같은 결과를 나타내었다. 이러한 결과는 천속단의 골다공증을 비롯한 골질환 치료의 후보물질로써의 가능성을 제시하였다. Many bone-related diseases such as osteoporosis, rheumatoid arthritis are occurred by excessive bone resorbing activity of osteoclasts. Recently, many studies have been proceeded to find out the new therapeutic materials from natural products of plants. Phlomis umbrosa Turcz, one of the natural products of plants has been known to improve bone health. However, the precise effects and treatment mechanisms of Phlomis umbrosa Turcz about bone diseases has been unknown. So, we examined the effects of Phlomis umbrosa Turcz on expression of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) and bone resorption. Also, we investigated the treatment mechanisms of Phlomis umbrosa Turcz relating to osteoclast differentiation. Here, we showed that Phlomis umbrosa Turcz significantly suppressed RANKLinduced osteoclast differentiation and bone resportion. Furthermore, Phlomis umbrosa Turcz suppressed the activation of NF-κB in bone marrow macrophage treated RANKL and M-CSF. The mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP) in BMMs was inhibited by Phlomis umbrosa Turcz. Integrin αν, β3 relating to cell adhesion and dendritic cell-specific transmembrane protein (DC-STAMP) relating to the structure of filamentous actin (F-actin) ring and cathepsin K relating to bone resorbing activity are disrupted too. These results suggest that Phlomis umbrosa Turcz will be a good materials to treat bone diseases like osteoporosis.

        • KCI등재후보
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          윌슨병 환자에서 다발성 섬유선종을 동반한 거대유방증 1예

          김훈혁,성기영,백종민,이도상,김욱,박일영,원종만,Hoon Hyuk Kim,M.D.,Gi Young Sung,M.D.,Jong Min Baek,M.D.,Do Sang Lee,M.D.,Wook Kim,M.D.,Il Young Park,M.D. and Jong Man Won,M.D. 대한갑상선-내분비외과학회 2004 The Koreran journal of Endocrine Surgery Vol.4 No.2

          '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

          Wilson's disease is an autosomal recessive disorder of copper metabolism in individuals with mutant ATP7B genes. Impairment of normal excretion of hepatic copper results in toxic accumulation of the metal in liver, brain and other organs. Clinical manifestations include hepatic, neurologic or psychiatric disturbances. Penicillamine, as a chelator of copper, is the drug of choice in the treatment of Wilson's disease but after treatment of penicillamine, granulocytopenia, thrombocytopenia, the nephrotic syndrome, Goodpasture's syndrome, pemphigus vulgaris or pleural effusion may supervene. We report a case of macromastia with multiple fibroadenomas in a patient who was treated with penicillamine for Wilson's disease. (Korean J Endocrine Surg 2004;4:115-118)

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