엘더베리 복합추출물이 함유된 프로바이오틱스 효능 평가

홍준성(Jun Sung Hong),배일권(Il kwon Bae) 한국동물보건학회 2023 한국동물보건학회지 Vol.2 No.4

Since COVID-19 pandemic, interest in lactic acid bacteria is increasing as a major health functional food as people recognize the importance of enhancing immunity in terms of physical and mental health of humans as well as pets. We aim to evaluate the efficacy of probiotics that can help improve immunity formulated using elderberries, chicory, bananas, and grains in vitro. Elderberries, chicory roots, and banana complex extracts were fermented using lactic acid bacteria and grains were added to use as samples for this study. Elderberry, chicory root, and banana complex extracts were fermented using lactic acid bacteria and grains were added to use as samples in this study. The antioxidant activity, cell viability, NO production, and inflammatory cytokine production were measured by ABTS radical scavenging activity, MTT assay, and nitrite (NO2-) measurement using LPS-stimulated raw264.7 cells. For evaluating the antioxidant efficacy using ABTS scavenging ability, it was confirmed that the radical scavenging ability was excellent performance depending on the concentration of the sample and the MTT assay performance result also did not affect the cell viability. The NO production inhibitory effect was confirmed depending on the concentration of cells induced by LPS. In addition, the expression amounts of TNF-α and IL-1β decreased as the indicators of cell anti-inflammatory efficacy. The antioxidant and anti-inflammatory activities of the sample used in this study can be identified, and it seems that it can be used as a material with the function of an immune-enhancing aid against diseases such as reduced immunity.

혈액검체에서 분리된 Streptococcus oralis 의 항균제 내성

배일권(Il Kwon Bae) 한국구강보건과학회 2017 한국구강보건과학회지 Vol.5 No.2

A rapid increase in antimicrobial resistant bacteria has become a serious problem in many countries including Korea. Streptococcus oralis is an early colonizer of the oral cavity that contributes to dental plaque formation. Many different genotypes can coexist in the same individual and cause opportunistic infection such as bacterial endocarditis. The aim of this study was to determine the prevalence of antimicrobial resistance among blood culture isolates of S. oralis in Korea. S. oralis had been isolated during the period from January to December in 2013. The resistance rate of 36 clinical isolates of S. oralis were 25.0% to penicillin, 22.2% to ampicillin, 19.4% to cefotaxime, 19.4% to cefepime, 2.8% to meropenem, 5.5% to clindamycin, 27.8% to chloramphenicol, 47.2% erythromycin, and 47.2% to tetracycline. None was resistant to vancomycin. Strict infection control and active surveillance program will be required to manage the antimicrobial resistance problem.

거주공간 내 그람음성 세균 분포 현황

배일권(Il Kwon Bae),강두호(Doo Ho Kang),김선민(Seon Min Kim),배영민(Yeong Min Bae) 한국구강보건과학회 2017 한국구강보건과학회지 Vol.5 No.1

Indoor environments of house and family member can become contaminated with potential variety bacteria. The aim of this study is to accesses the identification and distribution of indoor environments of house contaminated bacterial strains. We sampled living room floor, bathroom floor, bathroom knob, bottom of bathtub, refrigerator, refrigerator handle, desk, table, sink, bottom of shoes rack, hand of family member, nose of family member, and toilet bowl. All samples were cultured on blood agar media (BAM) and MacConkey agar media (MAM). The colonies grown on each media were identified with matrix-assisted laser desorption/ioniazation-time of flight mass spectrometry (MALDI-TOF) using MALDI Biotyper and 16S rDNA sequencing. Fifty-five isolates on BAM and MAM were identified as Acinetobacter soli (n=8), Acinetobacter lwoffii (n=6), Acinetobacter baylyi (n=3), Acinetobacter pittii (n=3), Acinetobacter baumannii (n=3), Acinetobacter junii (n=2), Acinetobacter radioresistnes (n=1), Aeromonas hydrophila (n=3), Aeromonas jandaei (n=1), Enterobacter cloacae (n=5), Enterobacter aerogenes (n=4), Klebsiella pneumoniae (n=1), Moraxella osloensis (n=1), Massilia timonae (n=1), Pseudomonas oryzihabitans (n=5), Pseudomonas aeruginosa (n=2), Pseudomonas mosselii (n=1), Pseudomonas stutzeri (n=1), Raoultella ornithinolytica (n=2), Serratia marcescens (n=2). These results suggest that indoor environments of house and family members were contaminated with variety nomal flora and nosocomial bacteria.

국내 개, 고양이, 사람 및 환경에서 유래된 항생제 내성 그람음성세균

홍준성(Jun Sung Hong),배일권(Il kwon Bae) 한국동물보건학회 2023 한국동물보건학회지 Vol.2 No.3

옥수수수염 추출액의 Klebsiella pneumoniae에 대한 항균활성

강현경(Hyun-Kyung Kang),배일권(Il Kwon Bae) 한국생명과학회 2015 생명과학회지 Vol.25 No.12

Klebsiella pneumoniae는 인간의 피부, 구강, 호흡기, 요로, 장관 등에서 일반적으로 존재하는 상재세균이다. 하지만 지역사회획득 폐렴의 주요 원인이 되는 기회감염균이기도 하다. 옥수수수염은 K. pneumoniae, Staphylococcus aureus, Bacillus subtilis, Shigella spp., Salmonella spp., Escherichia coli, Pseudomonas aeruginosa 등 병원성 세균에 대해 항균활성이 있는 것으로 알려져 있다. 본 연구에서는 옥수수수염 농축액의 K. pneumoniae에 대한 항균능을 확인하고자 하였다. 이를 위해 K. pneumoniae ATCC 13883, broad-spectrum β-lactamase (BSBL) 생성균주, exteded-spectrum β-lactamase (ESBL) 생성균주 및 carbapenemase 생성균주를 수집하였다. 항균제 감수성 검사는 디스크 확산법을 사용하였고 내성유전자는 PCR 증폭과 염기서열 분석을 통해 확인하였다. 또한 항균활성 실험을 위하여 옥수수수염 농축액을 이용하여 MacConkey agar 평판배지(50%, 100%)를 제조하였고 균주의 성장억제를 확인하기 위하여 제조된 배지에 K. pneumoniae를 평판도말하여 37℃ 항온기에서 18시간동안 배양하였다. 수집된 K. pneumoniae는 SHV-1 (n=8), SHV-2a (n=8), SHV-5 (n=2), SHV-11 (n=2), SHV-12 (n=18), TEM-1 (n=10), CTX-M-3 (n=2), CTX-M-14 (n=2), CTX-M-15 (n=1), GES-5 (n=5), KPC-2 (n=6), KPC-3 (n=4) 및 NDM-1 (n=2)을 보유하고 있었다. 시험에 사용된 K. pneumoniae 균주 가운데 K. pneumoniae ATCC 13883균주에 대해 항균활성이 있었으나 BSBL, ESBL 및 carbapenemase 생성균주에 대한 항균활성을 확인할 수는 없었다. 따라서 옥수수수염 추출액은 K. pneumoniae에 의한 지역사회획득 감염증 예방과 회복에 도움을 줄 수 있을 것으로 생각된다. Klebsiella pneumoniae is found in the normal flora of the skin, mouth, respiratory tract, urinary tract, and intestines in human. However, the stain is opportunistic pathogen, which is the causative agent of community acquired pneumonia. Corn silk has been known to be effective for antimicrobial activity against pathogenic bacteria, including K. pneumoniae, Staphylococcus aureus, Bacillus subtilis, Shigella spp., Salmonella spp., Escherichia coli, Pseudomonas aeruginosa, et al. In this study we focused on the antimicrobial properties of con silk water extract of K. penumoniae. K. pneumoniae isolates K. pneumoniae ATCC 13883 and broad-spectrum β-lactamase (BSBL), exteded-spectrum β-lactamase (ESBL), carbapenemase- producers. Antimicrobial susceptibilities were determined by the disk diffusion method. Searches for bla genes were performed by PCR amplication and direct sequencing. MacConkey agar plate medium was prepared using the corn silk extracts (50% or 100%) instead of distilled water for antimicrobial activity test. The microbial growth inhibitory potential of K. pneumoniae was determined by using the MacConkey agar plate spreading method, and the plate was incubated 18 hr at 37℃. Genes encoding β-lactamases including SHV-1 (n=8), SHV-2a (n=8), SHV-5 (n=2), SHV-11 (n=2), SHV-12 (n=18), TEM-1 (n=10), CTX-M-3 (n=2), CTX-M-14 (n=2), CTX-M-15 (n=1), GES-5 (n=5), KPC-2 (n=6), KPC-3 (n=4), and NDM-1 (n=2) were detected. The corn silk extract showed significantly antimicrobial activity against K. pneumoniae ATCC 13883, but BSBLs, ESBLs, and carbapenemase producers were not. Therefore, corn silk extract is thought to be able to assist in the prevention and rapid recovery of infectious disease caused by K. pneumoniae.

부산의 실내환경에서 분리된 항생제 내성유전자

홍준성(Jun Sung Hong),배일권(Il Kwon Bae) 한국동물보건학회 2023 한국동물보건학회지 Vol.2 No.3

국내에서 개발된 기구소독용 발포정의 슈퍼박테리아에 대한 살균력 평가

정소영(So-Young Jung),배일권(Il Kwon Bae) 한국구강보건과학회 2016 한국구강보건과학회지 Vol.4 No.1

Dental Clinic staffs are exposed to the risk of cross infection due to patient s blood, saliva and contaminated dental instruments. This study was designed to evaluate the performance of AOS Zero Germ (AOS Co. Ltd) effervescent labware wash tablet. A total of six types carbapenemase-producing Gram negative bacteria (KPC-2-producing Klebsiella pneumoniae , KPC-3-producing K. pneumoniae, NDM-1-producing K. pneumoniae , VIM-2-producing Enterobacter cloacae , VIM-2-producing Pseudomonas aeruginosa , OXA-23-producing Acinetobacter baumannii ), methicillin-resistant Staphylococcus aureus , vancomycin-resistant Enterococcus faecium were prepared in this study. All the isolates were exposed to the distilled water prepared with AOS Zero Germ. After the exposure the mixture of bacteria and AOS Zero Germ was inoculated onto MacConkey agar plate or blood agar plate and cultured at 37℃. All isolates were killed immediately of an exposure to AOS Zero Germ. It may be recommended that AOS Zero Germ can be used as an effective labware washer for clinical laboratory and an intermediate-level disinfectant for hospital infection control.

부산지역 치과병원의 환경과 종사자의 원내감염 세균 오염

정소영(So Young Jung),박민아(Min-Ah Park),정혜인(Hye-In Jung),배일권(Il Kwon Bae) 한국구강보건과학회 2016 한국구강보건과학회지 Vol.4 No.1

Dental hospital environments and staffs can become contaminated with potentially variety pathogenic bacteria and have a possibility of causing cross infections. The aim of this study is to assess the identification and dissemination of dental hospital contaminated bacterial strains. We sampled computer keyboard, dental gown, light handle, mobile phone, nasal cavity, office phone, scaler, 3-way syringe, dental spittoon, handpiece, staff hands, and headrest. All the samples were cultured on blood agar plate (BAP) and MacConkey agar plate. The colonies grown on each plate were identified with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF) using MALDI Biotyper. Sixty-three strains on BAP growth that were identified as Arthrobacter castelli (n=1), Corynebacterium pseudodiphtheriticum (n=2), Corynebacterium propinquum (n=1), Lactobacillius fructivorans (n=1), Micrococcus luteus (n=2), Staphylococcus aureus (n=11), Staphylococcus capitis (n=2), Staphylococcus epidermidis (n=34), Staphylococcus haemolyticus (n=3), and Staphylococcus warneri (n=4). Sixty-nine strains on MacConkey agar plate were identified as Acidovorax delafieldii (n=3), Acidovorax temperans (n=3), A. baumannii (n=2), Acinetobacter johnsonii (n=3), Acinetobacter lwoffii (n=3), Acinetobacter pittii (n=3), Acinetobacter radioresistens (n=1), Acinetobacter spp. (n=1), Chryseobacterium spp. (n=1), Citrobacter freundii (n=2), Comamonas testosteroni (n=1), Cupriavidus metallidurans (n=1), Delftia acidovorans (n=1), Enterobacter aerogenes (n=6), Enterobacter cancerogenus (n=1), Klebsiella oxytoca (n=2), K. pneumoniae (n=4), Klebsiella spp. (n=1), Moraxella osloensis (n=2), Moraxella spp. (n=2), P. aeruginosa (n=4), Pseudomonas asplenii (n=1), Pseudomonas entomophila (n=1), Pseudomonas jessenii (n=1) Pseudomonas luteola (n=1), Pseudomonas monteilii (n=2), Pseudomonas oryzihabitans (n=1), Pseudomonas rhodesiae (n=3), Rhizobium tumefaciens (n=1), Serratia marcescens (n=2), Sphingomonas paucimobilis (n=1), Sphingomonas spp. (n=3), Stenotrophomonas maltophilia (n=2), and Stenotrophomonas spp. (n=2). Our results showed that dental hospital environments and staffs were contaminated with variety nosocomial bacteria.

일부지역 근로자의 구취측정정도에 따른 구강건강관리행태와 주관적 구강건조증

장경애(Kyeung-Ae Jang),강현경(Hyun-Kyung Kang),배일권(IL Kwon Bae),이숙정(Sook Jeong Lee) 한국구강보건과학회 2014 한국구강보건과학회지 Vol.2 No.1

We investigated subjective conditions of dry mouth and health management behaviors of the oral cavity according to the bad breath measurements. This results will apply to increase habits of appropriate oral health management and prevention of bad breath. A survey was conducted from May 4 through July 29, 2013, by using structured, self-administered questionnaires. All statistical analyses were performed using SPSS. Population of bad breath 39 value less than or equal to the amount of drinking in response characteristics of normal according to the measured value of BB checker and 1-5 cups was higher than the population of bad breath value of 40 or more. Population who answered brushing the number of daily three or more times in health care behavior of the oral cavity in accordance with the measured value of BB checker was higher than the population of bad breath value more than 40 groups of bad breath values 3 9 below, (p<0.05). In the population answered to use interdental brush was higher than the population of bad breath value more than 40 groups of bad breath values 3 9 below, (p<0.05). Population who answered when the toothpaste, brushing the tongue and was higher than the population of bad breath value more than 40 groups of bad breath values 3 9 below (p<0.01). It is necessary for systematical managemental programs for prevention of bad breath. Conditions of dry mouth and oral heath care actions were related to measurements of the BB checker.

부산지역 치과환경에서 분리된 coagulase-negative staphylococci의 특성

정혜인(Hye-In Jung),정소영(So Young Jung),박인달(Indal Park),배일권(Il Kwon Bae) 한국생명과학회 2016 생명과학회지 Vol.26 No.2

본 연구는 치과 병의원 진료실 주변환경과 치과종사자의 휴대전화에서 분리된 coagulase-negative staphylococci(CNS)의 분리율과 항균제 내성양상 및 분자 역학적 특성을 분석하고자 하였다. 2014년 12월부터 2015년 1월까지 부산지역 10개의 치과 병의원에서 총 154개의 샘플을 수집하여 MALDI-TOF분석법을 통해 동정하였다. 항균제 감수성검사는 디스크 확산법을 시행하였고 mupA, mecA 유전자 보유현황 및 SCCmec type은 PCR과 염기서열분석에 의해 결정하였다. 154개의 샘플 중 10개(6.5%)에서 CNS 균주(Staphylococcus epidermidis 5주, Staphylococcus capitis 2주, Staphylococcus warneri 2주, Staphylococcus haemolyticus 1주) 가 분리되었다. 항균제 감수성검사에서 penicillin 10주, mupirocin 6주, gentamicin 5주, tetracycline 3주 및 cefoxitin과 erythromycin 2주가 내성이었고 clindamycin, ciprofloxacin, teicoplanin, trimethoprim-sulfamethoxazole에 내성인 세균은 없었다. 2개의 CNS균주(S. warneri, S. haemlyticus)에서 mecA 유전자가 검출되었고 1개의 CNS균주(S. epidermidis)에서 mupA가 확인되었다. Methicillin 내성 CNS균주 가운데 1주는 SCCmec I형이었고 1균주는 SCCmec의 유전형이 구분되지 않았다. 본 연구를 통하여 다약제 내성을 보이는 CNS균주가 더 이상 우리나라 치과병의원 환경에서 드물지 않음을 알 수 있었다. Coagulase-negative staphylococci (CNS) have recently become the bacteria most frequently found in clinical infections. The aim of this study was to investigate the prevalence, antimicrobial susceptibilities, and molecular characteristics of CNS isolates from dental clinic environments in Busan, Korea. One hundred and fifty-four samples were collected from 10 dental clinics and dental hospitals in Busan from December 2014 to January 2015. Species were identified by matrix-assisted laser desorption/ionization–time-of-flight. Antimicrobial susceptibility was determined by disk diffusion methods. A polymerase chain reaction was performed to detect mecA, mupA gene, and SCCmec types. Of the 154 samples, 10(6.5%) isolates were identified as CNS (5 Staphylococcus epidermidis, 2 Staphylococcus capitis, 2 Staphylococcus, and 1 Staphylococcus haemolyticus). Among the 10 isolates, 6 were resistant to penicillin, 5 were resistant to gentamicin, 3 were resistant to tetracycline, and 2 were resistant to cefoxitin and erythromycin. However, clindamycin, ciprofloxacin, teicoplanin, and trimethoprim-sulfamethoxazole resistant isolates were not present. Genes encoding mecA were detected in 4 (2 S. warneri and 2 S. haemolyticus) isolates, and mupA in 1 (S. epidermidis) isolate. One methicillin-resistant CNS (S. warneri) isolate was determined as being of the SCCmec type I. It is concluded that CNS resistant to various antimicrobial agents was widely distributed in dental clinic environments in Korea.