기능성 자기공명영상을 이용한 뇌기능 영상

배선준 ( Sun Joon Bai ) 대한소화기기능성질환·운동학회 2004 Journal of Neurogastroenterology and Motility (JNM Vol.10 No.1

약제내성 Mycobacterium tuberculosis의 rpoB 유전자 분석과 클로닝 발현

최은경,권태동,배선준,조해선,홍성갑,Choi, Eun Kyeong,Kweon, Tae-Dong,Bai, Sun-Joon,Cho, Hae Sun,Hong, Seong-Karp 한국정보통신학회 2013 한국정보통신학회논문지 Vol.17 No.4

마산병원과 결핵연구원에서 rifampin 내성균주를 확보하여 기존의 전통방법으로 검사된 항결핵제 내성균의 rifampin 내성관련 유전자인 rpoB (RNA polymerase beta subunit)의 변이를 DNA 염기서열 분석방법에 의하여 분석하였다. 그 결과 국내에서 분리되는 결핵균에서는 기존에 보고된 rpoB 변이부위와는 다른 위치의 DNA 염기 변이가 확인되었고 국내에서 여러 내성 결핵균주에서 변이가 발견되고 있지만 이러한 변이가 리팜핀 내성을 실제로 유발하는지 실험적으로 검증된 바는 없었다. 따라서 이러한 특이 부위의 rpoB변이들이 실제로 리팜핀 내성을 유발하는가를 확인하기 위하여 내성 결핵균주들의 rpoB 변이유전자를 polymerase chain reaction(PCR)으로 증폭하고 이것을 리팜핀 감수성 결핵균주에 cloning(클로닝)하고 발현시켜 rpoB 변이가 리팜핀에 대한 내성을 발생시켰음을 실험적으로 확인하였다. Using DNA sequencing method, we analyzed mutations of rpoB (RNA polymerase beta subunit) rifampin-resistant Mycobaterium tuberculosis strains which were identified by conventional test at Masan National Hospital and The Korean Institute of Tuberculosis. Though it has been reported different mutations of rpoB region of rifampin-resistant M. tuberculosis strains in the south of Korea, it is not confirmed whether these mutations of rpoB region actually express rifampin resistance through experiment. We confirmed experimentally these mutations of rpoB region of M. tuberculosis strains induced rifampin-resistance through ampified rpoB by polymerase chain reaction (PCR) and cloning of mutant rpoB into rifampin sensitive-M. tuberculosis strain.

침의 진통효과: 체성감각신경자극으로 유도된 진통작용에 대한 기능성자기공명영상장치를 이용한 연구

조장희,황선출,손영돈,강창기,배선준,이언정,성강경,박태석,김영보,민훈기,Cho, Zang-hee,Hwang, Seon-chool,Son, Young-don,Kang, Chang-ki,Wong, Edward K.,Bai, Sun-joon,Lee, Un-jung,Sung, Kang-kyung,Park, Tae-seok,Kim, Young-bo,Min, Hoon 대한침구의학회 2004 대한침구의학회지 Vol.21 No.2

Objective : Physiological evidence regarding acupuncture's effect in human patients is not yet well established, despite considerable evidence for its therapeutic efficacy. Besides target or disease specificity of acupuncture, acupuncture analgesia (AA) appears to be another large subclass that poses many questions, such as whether there is point specificity with respect to which acupoint is most effective for a particular condition. Methods : We observed brain activation with functional magnetic resonance imaging (fMRI) using a set of stimuli that consist of pain, pain following Meridian acupuncture, and pain following Sham acupuncture. Results : Among the new observations, the most interesting fact is that data sets of both Meridian acupuncture and Sham acupuncture show decreased activation of the same brain areas related to the pain processing signals. Present functional MRI study demonstrate two important biological observations that could elucidate AA mechanism in human participants: the effects of acupuncture occur through mediation of the higher brain areas. Sham acupuncture stimulation appears to be almost as effective as traditional Meridian acupoint stimulation, suggesting that acupuncture is not entirely point specific. Decreased activation in the limbic paleo cortical areas appears to be the probable neurological manifestation of AA and strongly implies that acupuncture stimulation inhibits the transmission of ascending pain signals to the higher cortical areas by the previously known descending pain inhibitory circuit. Conclusion : We, therefore, a hypothesized that this pain inhibitory circuit is initiated and mediated via the broad sense Hypothalamus Pituitary Adrenal (BS HPA) axis in conjunction to the "sensory stimulation."

백서의 관절염 모델에서 KHA-30의 통증 억제 효과

이배한 ( Bae Hwan Lee ),신양식 ( Yang Shik Shin ),조형준 ( Hyung Joon Cho ),심인섭 ( In Sop Shim ),이혜정 ( Hye Jeong Lee ),윤덕미 ( Duck Mi Yoon ),배선준 ( Sun Joon Bai ) 대한통증학회 2003 The Korean Journal of Pain Vol.16 No.2

슬관절경 시술 후 관절강내 모르핀 투여 효과

남순호,안은경,배선준,조범준,이행철 대한마취과학회 1998 Korean Journal of Anesthesiology Vol.35 No.1

Background : Opioids can produce potent antinociceptive effects by interacting with local opioid receptors in inflamed peripheral tissue. However, reports on pain relief with intra-articular morphine after arthroscopic knee operations are conflicting. In this study we examined the analgesic effects of the intraarticular administration of morphine after knee surgery. Methods : In a double-blind, randomized trial, we studied 26 patients who had received one of two injections at the end of surgery. The patients in group M(n=11) received 3 mg of morphine intraarticularly; those in group P(n=15), saline 20 ml intraarticularly as a placebo. Results : Patients in the morphine group had significantly lower pain scores throughout the 24-h postoperative period compared with those in the placebo group(P<0.05). There was less requirement for supplementary analgesics in the morphine group. Conclusions : Low doses of intraarticular morphine can significantly reduce pain after knee surgery without any systemic side effect (Korean J Anesthesiol 1998; 35: 120∼124)

노인에서의 고관절 성형술을 위한 요근구역 차단법

신양식,이기영,배선준 대한마취과학회 1995 Korean Journal of Anesthesiology Vol.28 No.3

Psoas compartment block for hip arthroplasty was evaluated clinically in 32, either sex, geriatric patients. To perform the block, the patient was placed in a lateral decubitus position and a line was drawn, connecting both iliac crests (intercristal line). 22G teflon-coated needle of 10cm-length was inserted at a point 3cm caudad to the intercristal line and 5cm lateral to the spinal midline on the side to be blocked. The nerve stimulator (1-3mA, 1Hz), Pajunk (GMBH, Geisingen, West-Germany), was used to elicit paresthesia or muscle twitching on the patient's ipsilateral leg. The block was performed with administration of a mixture of 15 ml of 1.5 % lidocaine, 15 ml of 0.5 % bupivacaine and 0.15 mg of epinephrine. The onset time of the block was 4.4±1.6 minutes and its duration was 3.8±1.5 hours. The mean value of the lowest mean arterial pressure during the first 1 hour after the injection of local anesthetics, 85.7±9.8 mmHg was lower, compared to the preanesthetic baseline value, 98.2±16.5 mmHg. The fai rate was 9.4% and any significant systemic toxicity was not seen. There were some intraoperative complaints such as the discomfort due to position bars. However, there was no remarkable problem intraoperatively. The results indicate that psoas compartment block is a recommendable safe anesthetic technique for hip arthroplasty in geriatric patients.

심장 이식술을 시행받은 환자의 저비중 척추마취

구민우,남용택,배선준,이행철 대한마취과학회 1998 Korean Journal of Anesthesiology Vol.35 No.5

Heart transplantation is an accepted procedure for treatment of end-staged cardiac failure. A return to near-normal quality on life can be expected in many patients with a nonrejecting cardiac allograft, and many of these patients will return to the operating room for noncardiac surgical procedures. Anesthesiologists should be alert to recognizing problems caused by the presence of infection in immunosuppressed patients, modes of presentation of rejection phenomena and how transplanted organs, notably significantly denervated ones, may behave and respond under the pathophysiologic circumstance that arise during surgery, resuscitation and intensive care. The use of regional techniques require adequate preloading to avoid exaggerated hypotension and aseptic technique to avoid infection. Hypobaric spinal anesthesia has some benefit. It does not depress cardiovascular and respiratory system and keep adequate venous return by trendelenberg position. We report herein a case of successfully undergone total hip replacement in a patient who had previously undergone orthotopic heart transplantation under hypobaric spinal anesthesia. (Korean J Anesthesiol 1998; 35: 999∼1002)

Desflurane 이 심근 소포체로부터의 Ca2+ 배출에 미치는 영향

김진수,이영석,심재광,남용택,배선준,문진천 대한마취과학회 2001 Korean Journal of Anesthesiology Vol.40 No.3

저비중 척추마취시 국소마취제의 용적이 마취 높이에 미치는 영향

김승준,한동우,남용택,배선준,오경미 대한마취과학회 2001 Korean Journal of Anesthesiology Vol.41 No.2

The Effect of the Injected Volume on the Spread of Spinal Anesthesia with Hypobaric Tetracaine Dong Woo Han, M.D., Seung Jun Kim, M.D., Sun Joon Bai, M.D. Kyeong Mee Oh, M.D., and Yong Taek Nam, M.D. Department of Anesthesiology, Yonsei University College of Medicine, Seoul, Korea Background: The properties of hypobaric spinal anesthesia are excellent in sensory and motor block with low concentration solutions, increased venous return, and hemodynamic stability. Much volume is needed with hypobaric spinal anesthesia because low concentration solutions are used. The purpose of this study was to examine the effects of the injected volume on the level of anesthesia in hypobaric spinal anesthesia during a total hip replacement. Methods: Twenty-seven adult patients scheduled for a total hip replacement were assigned randomly to two groups, one with 0.1% (n = 14), and the other with 0.2% (n = 13) hypobaric tetracaine spinal anesthesia. 8 mg of tetracaine was used for male patients and 7 mg for female patients. Hence the injected volumes were 8 ml for male and 7 ml for female patients in 0.1% solution and 4 ml for male and 3.5 ml for female patients in 0.2% solution, respectively. Epinephrine was mixed to either solution in concentrations of 1 : 200,000. The speed of injection was fixed at the rate of 0.2 ml/sec. Results: The height of sensory block with the 0.1% was one dermatome higher (T5.7) than with the 0.2% tetracaine solution (T6.7). The time for sensory block (11.4 min vs 12.3 min) and the time for motor block (10.0 min vs 15.0 min) with 0.1% was shorter than that with the 0.2% tetracaine solution. The duration of sensory block and the duration of motor bllock were not significantly different in either groups. Complete motor block was achieved in all patients. Conclusions: Both 0.1% and 0.2% hypobaric tetracaine spinal anesthesia are suitable for a total hip replacement. However we got better results with 0.1% than with 0.2% retracaine such as rapid and high sensory block with rapid motor block. (Korean J Anesthesiol 2001; 41: 159~164)

토끼에서 급성출혈성 쇽시 전해질용액 종류에 따른 비교

김정섭,남순호,남용택,배선준 대한마취과학회 1998 Korean Journal of Anesthesiology Vol.35 No.1

Background : The purpose of this study is to evaluate what kind of crystalloid solution could function as the best buffer and correct the metabolic acidosis most effectively in rabbits with hemorrhagic shock. Methods : Twenty eight rabbits were bled until mean arterial pressure(MAP) became 70% of control. Thirty minutes after hemorrhagic shock fluid resuscitation was started with either plasmalyte or Hartmann's solution or 0.9% normal saline until MAP returned to 90% of the control. Hemodynamic and blood gas study, plasma lactate and electrolyte concentration were measured before, during, and 30 minutes after recovery from shock. Results : The amount of shed blood to reduce MAP to 70% of control ranged 76∼87 ml. And the volume for fluid resuscitation was 274∼324 ml. There was no statistically significant difference among the three groups. The pH decreased during shock in all group and still decreased after resuscitation in Hartmann's solution and normal saline. But it increased significantly after resuscitaon in plasmalyte. Lactate was increased in all group during shock and decreased by 22 and 23 mg/dl after resuscitation in plasmalyte and normal saline. But it still increased by 40 mg/dl in Hartmann's solution. Serum potassium level decreased significantly after resuscitation with normal saline. Serum calcium level decreased significantly after resuscitation with plasmalyte and normal saline. Conclusion: With the above results the plasmalyte which has pH closer to that of normal blood might be able to avoid the metabolic acidosis and maintain acid-base equilibrium effectively after fluid resuscitation in acute hemorrhagic shock. (Korean J Anesthesiol 1998; 35: 16∼22)