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박종숙,Park, Jong-Suk 한국식품연구원 2010 한맛한얼 Vol.3 No.1
입춘이 지나면 살림을 사는 가정부인들은 한해의 시작으로 장 담그기를 준비한다. 우리 음식의 근간이 되어 온 전통장은 우리 밥상에서 조미료의 역할과 기본 반찬으로써의 역할을 충실히 해왔다. 간장과 된자을 따로따로 담그는 일본 장과는 달리 우리의 전통장은 대부분 한 번의 수고로 얻을 수 있다.
박종숙,김석원,인동수,은종선,Park, Jong-Suk,Kim, Suk-Weon,In, Dong-Su,Eun, Jong-Seon 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.1
고구마 품종 '율미'를 이용해 2,4-D 1.0 mg/L가 첨가된 MS 배지에 정단분열조직배양을 통하여 발생된 배발생 캘러스를 실험재료로 여러 가지 동결보호제를 처리하여 2-step method 에 의해 동결보존을 실시하였다. ABA 10m/L가 포함된 배지에서 전처리된 배발생 캘러스는 ABA 1.0mg/L 처리구보다 액체질소에 저장 후 생존율이 더 높게 나타났다. TTC방법과 FDA 염색법을 통해 초저온 보존 후에 생존을 확인한 결과 10mg/L의 ABA를 전처리 한 0.4M sucrose가 포함된 1.28M DMSO 처리구에서 46.8%의 가장 높은 생존율을 나타냈다. 캘러스를 1.0mg/L 2,4-D가 포함된 MS 고체배지에서 암배양한 결과 배양 4주일 후부터 1.28 M DMSO 단독처리구에서 캘러스가 생장하는 것을 육안 관찰할 수 있었으며 배양 8주일 후에는 배가 발생하였고, 0.1 mg/L 2,4-D+0.1 mg/L kinetin 혼용구에 2주일간 계대배양한 다음 MS기본배지에서 완전한 식물체로 재생되었다. Cryopreservation of embryogenic callus derived from apical meristem culture was attempted by slow prefreezing method (two-step method) with various cryoprotectants in sweetpotato cv. 'Yulmi' Precultured embryogenic calli on medium containing 10 mg/L ABA prior to slow prefreezing in liquid nitrogen indicated higher survival rate than 1.0 mg/L ABA preteatment. The cryoprotectant comprising 1.28 M DMSO in 0.4 M sucrose solution gave the best survival (over 46%) of sweetpotato cells exposed to liquid nitrogen as determined by TTC reduction and FDA staining method. Cryopreserved calli cultured on MS medium with 1.0 mg/L 2,4-D were grown for 4 weeks in the dark and induced embryos after another 4 weeks. They were subcultured on MS medium supplemented with 0.1 mg/L 2,4-D+0.1 mg/L kinetin for 2 weeks and regenerated into normal plantlets in MS basal medium.
Plasma Cytokine Analysis between Steroid Responders and Steroid Non-responders in Asthmatics
박종숙,( Young Suk Choi ),( An Soo Jang ),( Soo Hyun Kim ),( Jeong Seok Heo ),( Ji Da Choi ),( Si Young Lee ),( Eun Som Kim ),( Yoo Jung Lim ),( Choon Sik Park ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
Background: Inhaled corticosteroids (ICSs) have revolutionized the treatment of asthma and are now the mainstay of therapy for patients with chronic asthma. Interleukin (IL)-33 is a recently characterized IL-1 family cytokine and found to be expressed in inflammatory diseases, including severe asthma and inflammatory bowle disease. IL-32 is a proinflammatory cytokine originally described as a transcript termed NK4 found in activated natural killer (NK) cells and T lymphocytes. analysis of IL-32 and IL-33 cytokine may be important for understanding the pathobiology of steroid asthma at the protein levels. Objective We studied to identify biomarkers in steroid non-responder asthma as comparing protein profiles in plasma between steroid non-responders and steroid responers in asthmatics. Methods: We investigated that the IL-1b, IL-32 and IL-33 level of plasma between nomal control (n=10), steroid non responders (n=14) and steroid responders (n=20) in non-smoker asthmatics by ELISA. Results: We found that IL-33 level was lower significantly in the steroid non-responders (n=14) than the steroid responders(n=20) in asthmatics (p=0.01). But the level of IL-1b, IL-32 had no significant differences between the steroid responders(n=20) and the steroid non-responders (n=14) in asthmatics (p>0.05). Conclusion: This study suggested that the IL-33 level of plasma might be a biomarker of steroid non- respondes in asthmatics.
The "SCH Biobank": an Innovative Resource for Allergy and Respiratory Diseases Research
박종숙,( Tae Hoon Kim ),( Myoung Ran Lee ),( Eun Young Kim ),( An Soo Jang ),( Sung Woo Park ),( Do Jin Kim ),( Soo Taek Uh ),( Choon Sik Park ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
In these days, paradigms of medical science have been changed from the treatment of diseases to predictive and preventive medicine. Discovery of Biomarkers certainly play an important role in the development of new diagnostic and therapeutic strategies for the future personalized medicine. High-quality biospecimens from the patients and normal subjects are indis-pensible for successes of these strategies. Therefore, biobanks, particularly dealing with human - derived biomaterials with good clinical data, can provide key resources to the basic scientist and researchers for their translational studies and discovery of new biomarkers. The Soonchunhyang University Bucheon Hospital Human Resource Biobank is a Collaborative Biobank of Korea and has been supported by a grant from the Korea Health 21 R&D Project, Ministry of Health and Welfare and Family, Republic of Korea.Up to date, 30,000 samples were collected. The bank has covered common respiratory diseases including asthma, COPD, lung cancer, tuberculosis as well as rare ones such as pulmonary fibrosis. The bank has collected lung tissues, bronchial epithelium, plasma, DNA bronchoalveolar lavage fluids, sputum and urine from over 10,000 donors. The mission is to collect and provide the human derived bio-resources for the scientist and physicians trying to discover the molecular and cellular biomarkers on non-commercial and academic base. The Bioresources in the SCH Biobank have been distributed to several academic institutions and NIH in Korea and also to international institutions such as deCODE genetics.
Expression of Muc1 Gene in OZONE Induced Airway Injury
박종숙,( Da Jeong Bae ),( An Soo Jang ),( Sung Woo Park ),( Soo Taek Uh ),( Yong Hoon Kim ),( Choon Sik Park ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
Background: Tyrosine phosphorylation of MUC1 CT domain leads to inhibition of TLR signaling and down-regulation of inflammation. We tested, the expression of Muc1 gene in OZONE induced airway injury using animal models. Methods: The mice housed in whole-body exposure chambers were exposed to ozone concentrations of 2 ppm for 3 h (n=6) daily. After measurements of differential cell count were done in bronchoalveolar lavage (BAL) fluid, MUC1and proliferating cell nuclear antigen (PCNA) protein expression were observed in lung tissue using immunohistochemical stain (IHC). Results: 1. 3 weeks OZONE exposure increased the numbers of total cells, and neutrophils in BAL fluids of OZONE- sensitized/challenged mice in a time -dependent manner (P<0.05). But, on 14th days, the total cells and neutrophils were decreased again in 1weeks OZONE exposure mice. 2. MUC1 expressions were decreased in the 3 weeks OZONE exposure mice in a time -dependent manner (P<0.05). PCNA expressions were significantly increased in the 3 weeks OZONE exposure mice in a time -dependent manner until 14th days (P<0.05). 3. 1 weeks OZONE exposure were decreased in a time -dependent manner (P<0.05). But it was increased again after 14th days. PCNA expressions were increased in the OZONE exposure mice in a time -dependent manner until 7th days. But, with decreased after 14th days (P<0.05). Conclusions: These results indicate that MUC1 expressions by ozone were decreased, and this may lead to excessive airway inflammation.
말초성 담관암에 의한 속발성 식도이완불능증 및 유문부 협착 1 예
박종숙(Jong Suk Park),박효진(Hyo Jin Park),최성우(Sung Woo Choi),윤상욱(Sang Wook Yoon),이상인(Sang In Lee),박인서(In Suh Park) 대한소화기학회 2002 대한소화기학회지 Vol.39 No.5
We have infrequently experienced several cases of secondary achalasia caused by malignancy. Gastric carcinoma is the most common cause of secondary achalasia but other types of malignancy are rarely reported as a cause. Recently, we experienced a case of secondary achalasia and pyloric stenosis in a 62- year-old woman. She had a peripheral cholangiocarcinoma in the left lobe of the liver, which caused secondary achalasia by the direct invasion into the gastroesophageal junction. After 2 months, this malignancy caused pyloric stenosis by direct invasion of the gastric pylorus. (Korean J Gastroenterol 2002; 39: 370-374)