전염성 췌장 괴저 바이러스의 어류세포에의 감염에 미치는 리소솜 친화성 약염기의 영향

박정우,하영칠,정가진 ( Jeong Woo Park,Yung Chil Hah,GajIn Jeong ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.4

We investigated the effect of ammonium chloride, which is known to affect lysosome-mediated viral internalization, on the infectious of fish cells, CHSE-214, with Infectious Pancreatic Necrosis Virus (IPNV). Progeny production of IPNV was delayed 6 to 8 hours in IPNV infected CHSE-214 cells by the addition of NH₄C1 at the final concentration of 20 mM. The multiplication of IPNV, however, was no longer inhibited when NH₄C1 was present only for the first 4 hours after viral infection. Thus the inhibitory effect of NH₄C1 seems to be on some other steps but internalization. For the inhibitory effect of NH₄C1, time of addition is not very important, but the length of treatment is rather critical. Ammonium chloride attenuated the synthesis of almost all the proteins in IPNV-infected CHSE-214 cells. Thus the primary effect of NH₄C1 on virus-infected host cells is to inhibit the synthesis of proteins resulting in the delay of IPNV growth. Therefore, we conclude that IPNV penetration into CHSE-214 cells does not require the low-pH dependent activity of lysosome.

기술표준동향 - ISO 26000 사회적 책임 (Social Responsibility) 국제표준동향

박정우,Park, Jeong-Woo 기술표준원 2009 기술표준 Vol.89 No.-

한방 치료와 코어 운동을 병행한 퇴행성 슬관절염 환자 1례에 대한 증례보고

박정우,정수현,Park, Jung-Woo,Jeong, Su-Hyeon 척추신경추나의학회 2013 척추신경추나의학회지 Vol.8 No.2

Objectives : The objective of this study is to report the effect of Traditional Korean medical therapy and core exercise on osteoarthritis of knee joint by observing a clinical case study. Methods : Patient with a diagnosis of osteoarthritis of knee joint, was treated by acupuncture, herb medicine, physical theraphy and core exercise. Results : After treatment, Visual Analogue Scale(VAS), Korean version of the Western Ontario and McMaster Universities(KWOMAC), Pain Rating Score(PRS) were reduced from 5 to 2, from 25 to 16, from 55 to 27, and CENTAUR test results were improved. Conclusions : This study showed that Traditional Korean medical therapy and core exercise has meaningful effect on osteoarthritis of knee joint, and more researches should be followed.

국내분리 Aujeszky's disease virus의 실험적 감염 자돈에 대한 바이러스학적 연구

박정우,전무형,안수환,Park, Jeong-woo,Jun, Moo-hyung,An, Soo-hwan 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.2

To investigate the etiology, pathogenicity and virological properties of NYJ-1-87 strain of Aujeszky's disease virus (ADV) that was isolated from the diseased piglet in Korea, the virus at $10^{6.0}TCID_{50}/0.1ml$ was inoculated intranasally and subcutaneously into 30 to 35 days-old piglets. Results obtained through the experiments were summarized as follows. 1. Ten of the infected piglets were clinically observed for 15 days. On the 2nd day post-inoculation(pi), the signs of pyrexia, anorexia and convulsion were noted. On the 4th to 7th days pi, nervous signs of incoordination and intermittent spasm were shown in the most of piglets, and one out of 5 piglets infected intranasally was died with severe nervous signs at the 7th day pi. The signs became relieved on the 8th day pi and all of remainder were completely recovered on the 13th to 14th days pi. 2. In hematological study, prominent decrease in the number of total leukocyte and lymphocyte was shown in the ADV-infected piglets on the 6th day pi. On the 8th day pi, the cell numbers were slightly increased and returned to normal level on the 10th day pi. 3. Viral excretion of the ADV-inoculated piglets was examined by swabbing of nasal and oral cavities, and rectal feces. During the periods of the 3rd to 11th days pi, the virus was excreted intermittently from nasal and oral cavities, and rectal feces. The nasal excretions were shown the highest virus concentration of $10^{5.2}TCID_{50}/0.1ml$ at the 5th day pi. 4. Recovery of the inoculated virus from various organs of the piglets that were died or experimentally slaughtered was attempted, and the virus was isolated from the tissues of brain and tonsil by the cultured cell-inoculation method. The highest recovery rate was noted in the tonsil. By indirect immunofluorescence antibody assay using ADV-monoclonal antibody, the viral antigens were detected in tissues of spleen and liver as well as brain and tonsil on the 7th to 9th days pi. The virus was not isolated from blood and the tissues of lung and kidney throughout the experiments. 5. Titers of virus neutralizing antibody in the piglets experimentally infected with ADV became increased after the 6th to 9th days pi in both of intranasal and subcutaneous inoculation showing the highest titers of 64 to 128 on the 29th day pi. When the antibody levels were measured by radial immunodiffusion enzyme assay, the reactive diameter was enlarged to be positive after the 4th to 6th days pi in both of intranasal and subcutaneous inoculation showing the largest diameter of 13 to 14mm on the 29th day pi.

바위 자고새의 흑두병 발생예

박정우,전무형,조성환,조우영,Park, Jeong-woo,Jun, Moo-hyung,Cho, Sung-whan,Cho, Woo-young 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.2

The diseased Chuckar partridges that were raised at the wild bird-raising farms located in Ockchun, Chungbuk province, were submitted for diagnosis in the laboratory in July. 1990. The diseased partridges aged 3~4 weeks old were shown clinical signs such as anorexia, depression, white-colored or transparent-watery diarrhea and dropping of the head and wings. At necropsy, major gross lesions were found in the livers, ceca and lungs. Histopathologically, numerous histomonads were observed in the necrotic tissues of the livers and ceca. In the cecal specimens, it was found that the ameboid histomonads have flagellum and the size of 10~20 micrometer. The case was confirmed as histomoniasis due to the infection of Histomonas meleagridis.

인산 및 수증기 활성화에 의한 대나무 활성탄 제조 및 특성 연구

박정우,리황부,오창호,김승수,Park, Jeong-Woo,Ly, Hoang Vu,Oh, Changho,Kim, Seung-Soo 한국청정기술학회 2019 청정기술 Vol.25 No.2

Bamboo is an evergreen perennial plant, and it is known as one of the most productive and fastest-growing plants in the world. It grows quickly in moderate climates with only moderate water and fertilizer. Traditionally in Asia, bamboo is used for building materials, as a food source, and as versatile raw materials. Bamboo as a biomass feedstock can be transformed to prepare activated carbon using the thermal treatment of pyrolysis. The effect of process variables such as carbonization temperature, activation temperature, activation time, the amount of steam, and the mixing ratio of phosphoric acid and bamboo were systematically investigated to optimize the preparation conditions. Steam activation was proceeded after carbonization with a vapor flow rate of $0.8{\sim}1.8mL-H_2O\;g-char^{-1}\;h^{-1}$ and activation time of 1 ~ 3 h at $700{\sim}900^{\circ}C$. Carbon yield and surface area reached 2.04 ~ 20.59 wt% and $499.17{\sim}1074.04m^2\;g^{-1}$, respectively, with a steam flow rate of $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$ for 2 h. Also, the carbon yield and surface area were 24.67 wt% and $1389.59m^2\;g^{-1}$, respectively, when the bamboo and phosphoric acid were mixed in a 1:1 weight ratio ($700^{\circ}C$, 2 h, $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$). The adsorption of methylene blue into the bamboo activated carbon was studied based on pseudo first order and second order kinetics models. The adsorption kinetics were found to follow the pseudo second order model, which is governed by chemisorption. 대나무는 지구상에 존재하는 식물 중 적절한 기후와 토양조건에서 생산성이 가장 높고, 성장속도가 가장 빠른 다년생 식물로 알려져 있다. 전통적으로 아시아에서 대나무는 음식, 건축 및 다양한 재료로 활용되고 있다. 바이오매스 자원으로 대나무는 열분해과정을 거쳐 활성탄으로 제조될 수 있다. 본 연구에서는 탄화온도, 활성화 온도, 시간, 수증기의 양, 그리고 인산의 양 등을 변화에 따른 최적의 대나무 활성탄 제조 연구를 수행하였다. 대나무 탄화 후 수증기 활성화를 위해 $700{\sim}900^{\circ}C$의 온도, $0.8{\sim}1.8mL-H_2O\;g-char^{-1}\;h^{-1}$ 수증기 유량 범위에서 1 ~ 3 h 동안 활성화를 진행하였다. 수증기 유량을 $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$으로 2 h 동안 실험한 결과 활성탄 수율과 비표면적은 각각 2.04 ~ 20.59 wt%, $499.17{\sim}1074.04m^2\;g^{-1}$의 값이 나왔다. 대나무와 인산의 질량비를 1:1로 혼합한 후 $700^{\circ}C$에서 유량 $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$ 속도로 2 h 동안 활성화를 진행한 결과 활성탄 수율과 비표면적은 각각 24.67 wt%, $1389.59m^2\;g^{-1}$의 값이 나타냈다. 제조된 대나무 활성탄을 대상으로 메틸렌블루 흡착 실험을 통해 유사 1차, 2차 속도식 모델을 적용하였으며, 화학적 흡착을 의미하는 유사 2차 속도식에 따랐다.

진행성 위암에서 종양 맥관형성이 예후인자로서의 가치를 가지는가?

박정우(Jeong-Woo Park),이민효(Min-Hyo Lee),주정일(Jeong-Il Joo),주영태(Young-Tae Ju),정치영(Chi-Young Jeong),하우송(Woo-Song Ha),박순태(Soon-Tae Park),최상경(Sang-Kyung Choi),홍순찬(Soon-Chan Hong),권수인(Soo-In Kwon),이영준(Young-Jo 대한외과학회 1999 Annals of Surgical Treatment and Research(ASRT) Vol.57 No.3

Slocum 수중 글라이더의 유영 받음각에 대한 전산유동해석 및 항력계수 연구

박정우(Jeong-Woo Park),이정우(Jung-Woo Lee),최영호(Young-Ho Choi),서갑호(Kap-Ho Seo),서진호(Jin-Ho Suh),박종진(Jong-Jin Park) 한국해양공학회 2016 韓國海洋工學會誌 Vol.30 No.5

An underwater glider makes it easy to explore a wide area with low power. However, an underwater glider is difficult to use for rapid collection, because the surfacing location cannot be predicted after a dive. Thus, simulation research is needed to predict the swimming path. In this paper, based on research, a linearized equation is derived for the drag coefficient at each angle of attack by assuming the boundary conditions for the Slocum underwater glider and performing a computational flow analysis.

망막병증을 동반하지 않은 초기 제2형 당뇨병 환자에서 망막혈관 직경의 변화

박정우(Jeong Woo Park),배정훈(Jeong Hun Bae),송수정(Su Jeong Song),김준모(Joon Mo Kim) 대한안과학회 2022 대한안과학회지 Vol.63 No.1

목적: 망막병증이 동반되지 않은 초기 제2형 당뇨병 환자에서 망막혈관의 직경을 측정하고, 정상안과 비교하여 그 변화를 알아보고자 하였다. 대상과 방법: 제2형 당뇨병으로 진단받은 지 10년 이내의 환자 85명과 나이와 성별이 유사한 건강한 대조군 48명의 의무기록을 후향적으로 분석하였다. 모든 환자들은 산동 후 안저검사를 통해 당뇨망막병증이 동반되지 않음을 확인하였고, 안저카메라를 이용하여 안저촬영을 시행하였다. 망막혈관의 직경을 반자동으로 측정해주는 프로그램을 이용하여 중심망막동맥대응치, 중심망막정맥대응치, 동정맥비 값을 구하였다. 대조군도 환자군과 같은 방법으로 검사를 시행하고, 두 군을 비교 분석하였다. 결과: 환자군과 대조군의 중심망막동맥대응치는 각각 149.92 ± 21.29 μm, 144.85 ± 17.14 μm로 두 군 간에 유의한 차이가 없었고(p=0.137), 중심망막정맥대응치도 환자군과 대조군에서 각각 195.85 ± 22.94 μm, 196.69 ± 18.43 μm로 차이가 없었다(p=0.829). 그러나, 동정맥비는 환자군에서 0.768 ± 0.088이었고, 대조군은 0.737 ± 0.061로 두 군 사이에 유의한 차이가 있었다(p=0.031). 환자군에서 동정맥비는 당화혈색소와 유의한 상관관계가 있었으나(r=0.325, p=0.006), 체질량지수와는 관련이 없었다(p=0.478). 결론: 시신경유두 주위 동정맥비의 상승은 제2형 당뇨병 환자에서 관찰되는 망막혈관의 초기 변화로 생각되며, 이는 혈당의 증가와 밀접한 관련이 있다. Purpose: To evaluate the retinal vascular caliber in early type 2 diabetic patients without retinopathy. Methods: This retrospective study reviewed the medical records of 85 patients who were diagnosed with type 2 diabetes (age, 10-48 years) and sex-matched healthy controls. Dilated fundus examinations were performed to confirm the absence of diabetic retinopathy. Fundus photographs were acquired and analyzed using software that semi-automatically measured retinal vascular caliber. Central retinal arteriolar equivalent (CRAE), central retinal venular equivalent (CRVE), and arteriovenous ratio (AVR) were determined using the revised Parr-Hubbard formula. Healthy controls were examined using the same approach. Results: There were no significant differences between patients and controls in terms of CRAE (149.92 ± 21.29 μm and 144.85 ± 17.14 μm, respectively; p = 0.137) or CRVE (195.85 ± 22.94 μm and 196.69 ± 18.43 μm, respectively; p = 0.829). However, AVR was significantly higher in the patient group (0.768 ± 0.088 vs. 0.737 ± 0.061; p = 0.031). In the patient group, AVR was significantly correlated with hemoglobin A1c (r = 0.325, p = 0.006), but not with body mass index (p = 0.478). Conclusions: An increase in peripapillary AVR may be an early feature of retinal vessels observed in patients with type 2 diabetes, which is closely related to elevated blood sugar.

악성 위험지수 (Risk of Malignancy Index) 1을 이용한 골반내 종양의 분석

박정우 ( Jung Woo Park ),황성욱 ( Sung Ook Hwang ),박지현 ( Jee Hyun Park ),이병익 ( Byoung Ick Lee ),이정훈 ( Jeong Hoon Lee ),김기원 ( Ki Won Kim ),김경미 ( Kyoung Mi Kim ),정민재 ( Min Jae Jung ),윤내리 ( Nae Ri Yun ),송은섭 ( 대한폐경학회 2013 대한폐경학회지 Vol.19 No.1

Objectives: To assess the ability of risk of malignancy index (RMI) 1 to discriminate between benign and malignant pelvic masses. Methods: Between January 2007 and December 2010, 547 women with pelvic masses were evaluated. Their medical records are reviewed here retrospectively. The sensitivity, specificity and positive and negative predictive values of the cancer antigen (CA) 125 level, ultrasound findings and menopausal status in the prediction of malignant pelvic masses were calculated and compared individually or combined using the RMI 1. Results: The receiver operating characteristic (ROC) curves of CA 125, the ultrasound score and the RMI 1 were all found to be relevant predictors of malignancy. ROC analysis of the RMI 1, CA 125 serum levels, ultrasound score and menopausal status showed areas under the curves of 0.795, 0.782, 0.784 and 0.594, respectively. The RMI 1 was found to be statistically significantly correlated with menopausal status (P = 0.001), while not statistically significantly correlated with CA 125 (P = 0.628) or the ultrasound score (P = 0.541). The RMI 1 at a cut-off of 150 - with a sensitivity of 77.9%, specificity of 81.1%, positive predictive value of 51.7% and negative predictive value of 93.4% - showed the highest performance in determining the malignant tendency of pelvic masses. Conclusion: Accepting a RMI 1 cut-off value of 150 results in statistically more significant diagnostic criteria than menopausal status for the discrimination of benign and malignant pelvic masses. (J Korean Soc Menopause 2013;19:18-25)