GnRH의 맥동성 투여에 의한 남성 성선기능저하증의 치료

박기현,지용석,이병석,이보연,조동제,송찬호,이무상,최형기,이현철,허갑범,Park, Ki-Hyun,Jee, Yong-Seok,Lee, Byung-Seok,Lee, Bo-Yun,Cho, Dong-Jae,Song, Chan-Ho,Lee, Moo-Sang,Choi, Hyung-Ki,Lee, Hyun-Chul,Heo, Kab-Beom 대한생식의학회 1992 Clinical and Experimental Reproductive Medicine Vol.19 No.1

The effects of pulsatile GnRH therapy have been studied for the treatment of different forms of hypogonadotropic hypogonadism, including idiopathic hypogonadotropic hypogonadism and hypogonadism developed as a result of treatment with combination of surgery and irradiation for pituitary or hypothalamic tumor. GnRH was administered subcutaneously in a dose of $10{\mu}g$ every 2 hours with the pulsatile infusion pump. With GnRH therapy, all patients improved secretion of LH, FSH and testosterone. Testicular volumes increased. Spermatogenesis was induced in 8 patients. Pulsatile GnRH therapy is an effective treatment for idiopathic hypogonadotropic hypogonadism and can have a role in hypogonadism previously treated with combination of surgery and irradiation for pituitary or hypothalamic tumor.

CMP 패드 강성에 따른 산화막 불균일성(WIWNU)에 관한 연구

박기현,정재우,박범영,서헌덕,이현섭,정해도,Park, Ki-Hyun,Jung, Jae-Woo,Park, Boum-Young,Seo, Heon-Deok,Lee, Hyun-Seop,Jeong, Hae-Do 한국전기전자재료학회 2005 전기전자재료학회논문지 Vol.18 No.6

Within wafer non-uniformity(WIWNU) improves as the stiffness of pad decrease. We designed the pad groove to study of pad stiffness on WIWNU in Chemical mechanical polishing(CMP) and measured the pad stiffness according to groove width. The groove influences effective pad stiffness although original mechanical properties of pad are unchanged by grooving. Also, it affects the flow of slurry that has an effect on the lubrication regime and polishing results. An Increase of the apparent contact area of pad by groove width results in decrease of effective pad stiffness. WIWNU and profile of removal tate improved as effective pad stiffness decreased. Because grooving the pad reduce its effective stiffness and it makes slurry distribution to be uniform. Futhermore, it ensures that pad conforms to wafer-scale flatness variability. By grooving the top pad, it is possible to reduce its stiffness and hence reduce WIWNU and edge effect.

백작약약침(白芍藥藥鍼)이 백서(白鼠)의 위궤양(胃潰瘍)에 미치는 면역조직화학적(免疫組織化學的) 연구(硏究)

박기현,한상원,박재현,Park Ki-Hyun,Han Sang-Won,Park Jae-Hyun 대한침구의학회 1998 대한침구의학회지 Vol.15 No.2

In order to study the immunohistochemical effects of herbal drug-acupuncture of Radix paeoniae lactiflorae on gastric ulcer induced by HCl-aspirin in rats. this experiment was done by herbal drug-acupuncture to Wisu($B_{21}$). Chung-Wan($CV_{12}$), Chok-Samni($S_{36}$) loci to measure histological features of ulcer lesion, the change of numbers of parietal cell, chief celI, gastrin and somatostatin-immunoreactive cell. The obtained results were as follows; 1. The ulcerative lesions of gastric mucosa were decreased to WiSU($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 2. In the numbers of parietal cell, the most remarkable decrease was observed to Wisu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 3. In the numbers of chief cell, the most remarkable increase was observed to Wisu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 4. In the numbers of gastrin-immunoreactive cell, the most remarkable decrease was observed to Wiu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 5. In the numbers of somatostatin-immunoreactive cell, the most remarkable decrease was observed to Wisu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups.

Effects of 3-Methylcholanthrene on DNA Damages and Replication Inhibition in the Presence of Metabolic Activation System

박기현,이정섭,이형호,박상대,Park, Ki-Hyun,Lee, Jung-Sup,Lee, Hyung-Ho,Park, Sang-Dai 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2

CHO-KI 세포를 재료로 발암 전구물질인 3-methylcholanthrene(3-MC)에 의한 DNA사 절단, 비주기성 DNA합성 및 복제억제에 마치는 영향을 쥐의 간 상등액(5-15)과 NADPH를 조효소로 하는 대사활성계를 이용하여 알키리 유출법, 자기방사법 및 알카리 농도구배 초원심 분리법으로 조사하였다. 3-MC를 S-15으로 활성화시키지 않을 경우 24시간 처리에도 비주기성 DNA합성이나 DNA사절단을 거의 일으키지 않았다. 그러나 S-15과 함께 3-MC를 1시간 처리하였을 경우 비주기성 DNA 합성과 DNA단사 절단율이 농도증가에 따라 증가하였으며, hydroxyurea와 ara-C를 후처리 하였을 때 현저한 DNA단사 축적양상이 보였다. 그리고 유발된 단사절단의 회복에 있어서는 3시간 이상의 시간에도 대조군 수준으로 회복되지 않았다. 이러한 결과는 3-MC가 DNA상해요인으로 작용하기 위해서는 대사활성이 반드시 필요하며 자외선형의 상해유발 물질임을 암시한다. 또한, DNA복제양상은 농도에 비 례 DNA합성률이 억제되면서, $10^{-6}\;M$ 이하의 농도에서는 DNA복제 개시 (replicon initiation)가, 그 이상의 농도에서는 DNA복제개시와 DNA사 생장(chain elongation)의 동시 억제양상이 보였다. DNA strand breaks and replication inhibition by 3-methylcholanthrene (3-MC) were determined in the presence or absence of metabolic activation in CHO-KI cells. DNA strand breaks were induced in a small extent by 3-MC for 24 hr treatment. However, the single strand breaks were effectively induced by 3-MC for 1 hr treatment with the metabolic activation system. The production of strand breaks were remarkably increased by post-incubation with hydorxyurea and ara-C. At low doses below $10^{-6}\;M$, inhibition of replicon initiation was observed, at higher doses non-specific inhibition of both replicon initiation and chain growth was shown.

국립공원 내 공중화장실에 대한 탐방객 인식에 관한 연구 : 지리산국립공원을 중심으로

이호(Ho Lee),이승록(Seung-Rok Lee),박기현(Ki-Hyun Park),박은희(Eun-Hee Park),차진열(Jin-Yeol Cha),심규원(Kyu-Won Sim),강동원(Dong-Won Gang) 국립공원연구원 2011 국립공원연구지 Vol.2 No.1

본 연구는 공원 내 공중화장실에 대한 탐방객의 인식이 만족도에 미치는 영향을 규명하고자 수행하였다. 현지 설문조사는 지리산국립공원의 성삼재와 중산리 일원을 방문한 탐방객 292명을 대상으로 이루어졌다. 분석결과, 공중화장실의 관리상태, 외관, 디자인 요인 모두 탐방객의 공중화장실 이용 만족에 영향을 주는 것으로 분석되었으며, 그 중 관리상태가 가장 큰 영향을 주는 것으로 나타났다. 따라서 탐방객의 인식과 만족을 향상시키기 위해서는 공중화장실의 청결한 관리 및 유지, 악취 방지, 소모품의 원활한 공급 등에 우선순위를 두어 집중적으로 관리할 필요가 있는 것으로 나타났다. This study conducts to investigate the relationship between visitors’ perception and satisfaction. Data of 292 visitors were collected from Seongsamjae and Jungsanri in Jirisan National Park. The results showed that maintenance, outlook and design of public toilet are positively related to visitors’ satisfaction. One of the major research findings is that the maintenance of public toilet most affects visitors’ satisfaction. Therefore, the park managers need to put great emphasis on the cleanliness and maintenance, preventing stinks, consumable supplies of public toilet in order to enhance visitors’ perception and satisfaction.

DNA 상해와 복제억제에 미치는 3 - methylcholanthrene 의 영향

박기현,이정섭,이형호,박상대 ( Ki Hyun Park,Jung Sup Lee,Hyung Ho Lee,Sang Dai Park ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.2

DNA strand breaks and replication inhibition by 3-methylcholanthrene (3-MC) were determined in the presence or absence of metabolic activation in CHO-Kl cells. DNA strand breaks were induced in a small extent by 3-MC for 24 hr treatment. However, the single strand breaks were effectively induced by 3-MC for 1 hr treatment with the metabolic activation system. The production of strand breaks were remarkably increased by post-incubation with hydorxyurea and ara-C. At low doses below 10^(-6) M, inhibition of replicon initiation was observed, at higher doses non-specific inhibition of both replicon initiation and chain growth was shown.

폐경전 무월경 여성에 있어서 골밀도 측정에 관한 연구

박기현,이병석,이보연,조동제,송찬호,Park, Ki-Hyun,Lee, Byung-Seok,Lee, Bo-Yon,Cho, Dong-Jae,Song, Chan-Ho 대한생식의학회 1992 Clinical and Experimental Reproductive Medicine Vol.19 No.1

Bone mineral density was measured to assess whether the patients with premonopausal hypogonadism, comprised of 19 patients with hypogonadotropic hypogonadism(HH), 55 with premature ovarian failure(POF), 23 with hyperprolactinemia(HPLN), and 8 with Sheehan's syndrome. All aged from 20 to 39, were associated with some decrease in regional bone mass compared with that of 63 normal-associated with some decrease in regional bone mass compared with that of 63 normal-cycling control women matched with age and sex. Measurement of bone mineral density was carried out using Dual photon absorptiometry at four sites; femur neck, ward's triangle, trochanter and spine(L2-L4). Bone mineral density at all four sites were significantly decreased in patients with HH(p<0.01), POF(p<0.01). In hyperprolactinemic patients, the decrease in bone mass was significant at femur neck and Ward's triangle(p<0.05). The patients with POF were noted to be associated with significant bone loss when their duration of amenorrhea exceeded one year. In this study, the degree of loss of bone mass and the affected sites seemed to be different depending on the various types of hypogonadism and POF was noted have caused the appearance of loss of bone mass earlier than other thpes of hypogondism we experienced.

연마불균일도에 영향을 미치는 패드 표면특성에 관한 연구

박기현,박범영,김형재,정해도,Park, Ki-Hyun,Park, Boum-Young,Kim, Hyoung-Jae,Jeong, Hae-Do 한국전기전자재료학회 2006 전기전자재료학회논문지 Vol.19 No.4

Pad surface characteristics such as roughness, groove and wear rate of pad have a effect on the within wafer non-uniformity(WIWNU) in chemical mechanical polishing(CMP). Although WIWNU increases as the uniformity of roughness(Rpk: Reduced peak height) becomes worse in an early stage of polishing time, WIWNU decreases as non-uniformity of the Rpk value. Also, WIWNU decreases with the reduction of the pad stiffness, though original mechanical properties of pad are unchanged by the grooving process. In addition, conditioning process causes the inequality of pad wear during in CMP. The profile of pad wear generated by the conditioning process has a significant effect on the WIWNU. These experiments results could help to understand the effect of pad surface characteristics in CMP.

유도물질에 의한 휜쥐조직의 Aryl Hydrocarbon Hydroxylase 수준비교와 간장에서 Cytochrome P - 450s 의 동위효소 유도

박기현,김정렬 ( Ki Hyun Park,Chung Ryul Kim ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.1

The induction levels of microsomal aryl hydrocarbon hydrosylase (AHHase, EC 1.14.14.1) in tissues of Sprague-Dawley rats (male, 160-180g) were investigated after i.p. administration with 3-methylcholanthrene (3-MC), phenobarbital (Pb), catechol and ethyl alcohol, or these mixtures. 3-MC, 3MC+ethyl alcohol or Pb treatment significantly induced AHHase level in all the groups tested, whereas AHHase activities by treatment of ethyl alcohol or catechol had not reached that of 3-MC or Pb in liver. When rats were treated with mixture of 3-MC and catechol, kidney was the tissue most sensitive to induction of AHHase in all cases. Pb-induced P-450 content was greatly higher than that of 3-MC, but apparent AHHase induction was observed in the livers of rats treated 3-MC as compared to that of the corresponding Pb. There was because the patterns of induction of AHHase activities by Pb or 3-MC were not only very different, but induction levels of P-450 isozymes (P-450-a, P-450-b, P-450-c, P-450-d) from liver were also different. The Pb-monoclonal antibody inhibited about 30~ of the AHHase activities of Pb-induced liver microsome at a concentration of 200~g ascites fluid protein per reaction mixture (㎖) . The liver microsome from 3-MCtreated rats also were inhibited about 70% of the AHHase activity by 3-MC-monoclonal antibody. These results demonstrate that the high induction of 30% of P-450-b and 70% of P-450-c in total P-450s of rat liver microsomes by Pb or 3-MC treatment, respectively.

항진균능을 가진 불용성 인산 가용화 세균의 분리 및 특성

박기현,손홍주,Park, Ki-Hyun,Son, Hong-Joo 한국미생물학회 2006 미생물학회지 Vol.42 No.3

다기능성 농업용 미생물 제제를 개발하기 위하여 항진균능을 가진 불용성 인산 가용화 세균을 식물의 근권 토양으로부터 분리하였다. 분리균주의 분류학적 위치를 검토한 결과, Pseudomonas fluorescens RAF15로 동정되었다. P. fluorescens RAF15는 농작물의 잿빛곰팡이병균 Botrytis cinerea와 줄기썩음병균 Rhizoctonia solani의 생육을 억제할 수 있었다. 불용성 인산인 $Ca_3(PO_4){_2}$로부터 가용성 인산을 생성하기 위한 최적 배지 및 배양조건은 glucose 1.5%, urea 0.005%, $MgCl_2{\cdot}6H_2\;0.3%,\;MgSO_4{\cdot}7H_2O\;0.01%,\;CaCl_2{\cdot}2H_2O$ 0.01%, NaCl 0.05%, 배양온도 $30^{\circ}C$ 및 초기 pH 7.0이었으며, 최적조건에서 배양 5일 후 863 mg/L의 가용성 인산이 생성되었다. 불용성 인산 가용화는 유기산 생성에 의한 배양액의 pH 감소와 밀접한 관계가 있었다. P. fluorescens RAF15는 $10-35^{\circ}C$, 1-4% 염 농도 및 pH 2.0-11.0의 범위에서도 가용성 인산을 생성할 수 있었다. 본 균주는 $CaHPO_4,\;Ca_3(PO_4){_2}$ 및 hydroxyapatite에서 각각 971-1121 mg/L, 791-908 mg/L 및 844 mg/L의 가용성 인산을 생성하였다. 그러나 $FePO_2$ 및 $AlPO_4$의 경우, 생성된 가용성 인산의 농도는 각각 18 mg/L, 5 mg/L이었다. To develop multifunctional microbial inoculant, an insluble phosphate-solubilizing bacterium with antifungal activity was isolated from plant rhizospheric soil. On the basis of its morphological, cultural and physiological characteristics and Biolog analysis, this bacterium was identified as Pseudomonas fluorescens RAF15. P. fluorescens RAF15 showed antifungal activities against phytopathogenic fungi Botrytis cinerea and Rhizoctonia solani. The optimal medium composition and cultural conditions for the solubilization of insoluble phosphate by P. fluorescens RAF15 were 1.5% of glucose, 0.005% of urea, 0.3% $MgCl_2{\cdot}6H_2\;0.01%\;of\;MgSO_4{\cdot}7H_2O\;0.01%,\;of\;CaCl_2{\cdot}2H_2O$, and 0.05% of NaCl along with initial pH 7.0 at $30^{\circ}C$. The soluble phosphate production under optimum condition was 863 mg/L after 5 days of cultivation. The solubilization of insoluble phosphates was associated with a drop in the pH of the culture medium. P. fluorescens RAF15 showed resistance against different environmental stresses like $10-35^{\circ}C$ temperature, 1-4% salt concentration and pH 2-11 range. The strain produced soluble phosphate to the culture broth with the concentrations of 971-1121 mg/L against $CaHPO_4$, 791-908 mg/L against $Ca_3(PO_4){_2}$, and 844 mg/L against hydroxyapatite, respectively. However, the strain produced soluble phosphate to the culture broth with the concentrations of 15 mg/L against $FePO_4$, and 5 mg/L against $AlPO_4$, respectively.