한국건축의장에 대한 고민과 갈등

김흥곤,Kim, Heung-Gon 대한건축사협회 1980 建築士 Vol.1980 No.12

鐵道事故患者의 傷害傾向의 分析 및 統計的觀察

金興坤(Heung Gon Kim) 대한외과학회 1968 Annals of Surgical Treatment and Research Vol.10 No.12

자궁경부암의 Chemotherapeutic Response를 증가시키는 Baicalin Hydrate의 세포 내 신호전달 기전

김병륜 ( Kim Byeong Lyun ),김인숙 ( Kim In Sug ),고경희 ( Go Gyeong Hui ),이제중 ( Lee Je Jung ),김흥곤 ( Kim Heung Gon ),박래길 ( Park Lae Gil ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.10

Baicalin hydrate는 baicalin이 수화된 것으로 flavonoid계 물질로 PC-SPES의 주요성분이다. PC-SPES는 전립선암 환자와 전립선암을 가진 쥐들에 투여한 결과 환자들의 전립선특이성항원 수치를 낮추고 쥐의 전립선암 종양 크기를 줄인다고 알려져 있다. Baicalin은 항산화작용, 세포독성효과, 역전사효소의 억제에 의한 항바이러스효과 및 혈소판이나 백혈구 등의 골수유래세포, 상피세포, 신경세포 등에 함유되는 12-Lipoxyg Baicalin is flavonoid and major component of PC-SPES. Flavonoids including baicalin have been reported to not only function as anti-oxidant but also cause cytotoxic effect. Baicalin hydrate has been reported to induce cell death, however the mechanism by

난소에 발생한 Sertoli - Leydig Cell Tumor 1 예

김인숙(In Sook Kim),최정호(Jung Ho Choi),김신호(Sin Ho Kim),박현진(Hyun Jin Park),김흥곤(Heung Gon Kim),문형배(Hyung Bae Moon) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.6

The Sertoli-Leydig cell tumor is a gonadal tumor of sex-cord type, similar to that seen in the various phase of testicular development in the male. It is the most common type of all virilizing ovarian tumors and account for less than 0.5% of all ovarian tumors. This case has been presented of a 16 years old girl who had Sertoli-Leydig cell tumor of the right ovary. The clinical manifestation included deepening voice and hirsutism. The removal of the tumor, which proved to be a Sertoli-Leydig cell tumor, so we present it with a brief review of literature.

Nitroprusside가 인간정자의 생존력, 운동성, Reactive Oxygen Species 발생에 미치는 영향

민부기,이희민,김기석,이희섭,김흥곤,홍기연,이봉주,Min, Bu-Kie,Lee, Hee-Min,Kim, Ki-Seok,Lee, Hee-Sup,Kim, Heung-Gon,Hong, Gi-Youn,Lee, Bong-Ju 대한생식의학회 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.3

Objective: To analyze the direct effect of nitre oxide, generated from sodium nitroprusside, on sperm motility and reactive oxygen species. Design: Human sperm samples were treated to allow swim-up and washing. And the samples were devided into four aliquots. Each aliquot was incubated with either concentration at 0, 100nM, $10{\mu}M$, 1mM of nitroprusside. Intervention: Samples were measured chemiluminosence for reactive oxygen species of each aliquot with concentrations at 0, 100nM, $10{\mu}M$, 1mM of nitroprusside at allowing swim-up and washing of sperm. Main Outcome Measures: Percent motion parameters and viability were asse-ssed at 0, 3, 6, 12, 24 hours incubation. Results: The percent viablity was lower slightly in control group (50.2%) than that in sperm treated with 100nM of nitroprusside(57.5%) at 24 hours after incubation, while was reduced significantly in sperm with concentra-tion of $10{\mu}M(42.1%)$ and 1mM(21.3%)of nitroprusside at 6 hours after incubation. And the sperm treated with 1mM of nitroprusside was immotile totally at 6 hours after incubation. The straight line$(35.3{\pm}5.6%)$, the rapid forward$(37.2{\pm}6.4%)$ and the weak curvilinear velocity$(9.6{\pm}2.4%)$were more favorable comparing with those ($32.4{\pm}4.2%$, $30.0{\pm}7.8%$ and $18.0{\pm}4.6%$ respectively) in control group at 3 hours after incubation, but reduced significantly in sperm treated with $10{\mu}M$ and 1mM of nitroprusside. The levels of reactive oxygen species in control(700 c.p.m.) is lower significantly than that in each experimental groups of sperm treated with nitroprusside. And the levels of reactive oxygen species were 2200 c.p.m. in 100nM, 6200c.p.m. in $1{\mu}M$ and 12800c.p.m. in 1mM respectively. Conclusion: These results suggested that the concentration of 100nM of nitroprusside on sperm is beneficial to the maintanance of viablity and motile velocity, but detriment in high concentration of $10{\mu}M$ or 1mM of nitroprusside.

인간 난관세포와의 체외 공동배양과정에서 혈소판 활성요소가 생쥐배의 발달에 미치는영향

민부기,김기석,이희섭,홍기연,김흥곤,신무철,이찬근,최은하,Min, Bu-Kie,Kim, Kie-Seok,Lee, Hee-Sup,Hong, Kie-Youn,Kim, Heung-Gon,Shin, Mu-Cheol,Lee, Chan-Kun,Choi, Eun-Ha 대한생식의학회 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.1

There are a number of problems during the process of culture in vitro on fertilization and embryo development compared to those on in vivo counterparts. And the platelet activating factor (PAF), which is found not only in mammalian spermatozoa but also preembryos, is implicated on reproductive process. To improve the environment of culture on in vitro fertilization and embryo development, coculture using salpingeal epithelial cells has been considered to accept the better result on pregnancy rate. This study was designed to determine if two different culture systems, coculture alone and PAF treated coculture, are positive or negative influence on process of in vitro fertilization and embryo culture in mouse. The cell cleavage rate reached to 2-4 cell stage at 24 hours of culture is 56.81% (50/88) and 48.21%(54/112) respectively, in PAF treated group which is added PAF on coculture and in coculture group. But the rate of cells cleavage was similar in both group after 48 hours of culture. The rate of unfertilization after insemination of oocytes was higher in coculture group(55..53%) than in PAF treated group(42.37%). And in assessment of undeveped embryos, the rate of equalized cell block was similar on both, coculture alone (35.3%)and PAF treated coculture(35.5%). while unequalized cell block was higher rate in PAF treated coculture(19.4%) than coculture alone (11.8%). But the rate of cytoplasmic degeneration of undeveloped embryos was significantly higher in PAF treated coculture than coculture alone. In conclusion, we have observed that PAF treated coculture is superior in the rates of in vitro fertilization and early embryo cell cleavage compared to those in coculture alone, but there is no difference on the rates of embryo develpments, cell degeneration, cell quality in both PAF treated coculture and coculture alone when the embryo cells were continuosly cultured for 48 hours or more.

자간전증에서 혈청내 혈관내피 성장인자의 연구

김신호 ( Shin Ho Kim ),최기욱 ( Gee Uook Choi ),최정호 ( Jeong Ho Choi ),조해중 ( Hae Joong Cho ),박성남 ( Seong Nam Park ),홍기연 ( Gi Youn Hong ),김흥곤 ( Heung Gon Kim ),민부기 ( Boo Kee Min ) 대한주산의학회 2004 Perinatology Vol.15 No.3

목적 : 진단 기준에 따른 경증 전자간증과 중증 전자간증 임신부에서 혈관 내피 성장 인자가 전자간증의 중등도에 미치는 영향을 평가하기 위해서 본 연구을 실시하였다. 방법 : 1999년 1월 1일부터 2001년 6월 30일까지, 임신 28~40주 사이의 경증 전자간증 46명과 28명의 중증 전자간증 임신부를 대상으로 하였다. 각각의 경증과 중증 전자간증 임신부에서 혈청 내 간 효소치, 혈소판 수, 크리아티닌를 측정하였고, 태아의 제대동맥에서 수축/이완 비와 혈관 내피세포의 손상을 확인하기 위해 혈청 혈관 내피 성장인자의 농도를 측정하였다. 결과 : 간 효소치 상승, 혈수판 수 감소, 고크레아티닌은 경증 전자간증에 비해 중증 전자간증에서 보다 높은 비율을 보였다. HELLP증후군의 빈도는 중증 전자간증에서만 높은 비율을 보였다. 제대 동맥의 수축/이완의 비는 경증 전자간증 임신부의 4/46 (8.7%)에서 >3.0 이상이고, 중증 전자간증 임신부는 15/28 (53.6%)에서 3.0~4.5로 중증 전자간증에서 높은 빈도의 태반 혈류 저항을 보였다. 혈청 내에 혈관 내피 성장인자의 농도는 경증 전자간증에서 7.5±4.9 ng/mL, 중증 전자간증에서는 19.3±8.8 ng/mL로 경증 전자간증에 비해 중증 전자간증에서 통계적으로 유의한 차이를 보였다(p<0.05). 결론 : 제대 동맥의 수축/이완 비가 증가하는 것과 미세 혈관 투과성 및 혈액 응고성을 증가시키는 혈청 혈관 내피 성장인자 농도는 경증 전자간증에 비해 중증 전자간증 임신부에서 높게 측정 되었다. 따라서 혈청 혈관 내피 성장인자 농도 변화는 전자간증 임신부의 예후와 태아 발육부전을 예측하는 지표가 된다. Objective : The purpose of this study was to assess the impact of vascular endothelial growth factor (VEGF) in pregnancies with mild and severe preeclampsia. Methods : From January 1999 to June 2001, we studied the severity for pregnant women with pregnancy induced hypertension between 28 and 40 weeks gestation. In the mild (n=46) and severe preeclamptic women (n=28), the laboratory evaluation included liver function test, platelet counts, and serum creatinine. The systolic/diastolic (S/D) ratio of the fetal umbilical artery flow for placental resistance was measured by ultrasonographic doppler velocimetry. To detect the damage of vascular endothelial cells in all preeclamptic women, serum concentrations of VEGF were measured. Results : Severe preeclampsia had more elevated liver enzymes, thrombocytopenia, high creatinine than mild preeclampsia. HELLP syndrome (hemolysis, elevated liver enzymes, and low platelets) was encountered in 8/28 (28.6%) of severe preeclampsia. Fifteen out of twenty-eight cases (53.6%) in severe preeclampsia had elevated S/D ratio from 3.0 to 4.5 including 6 cases with absent end diastolic velocity, whereas 4/46 (8.7%) was elevated SD ratio (>3.0) in mild preeclampsia. Serum concentrations of VEGF were elevated in both mild (7.5±4.9 ng/mL, p<0.05) and severe preeclampsia (19.3±8.8 ng/mL, p<0.05) compared to normal pregnancy (0.5~2.1 ng/mL). Conclusion : The higher serum concentration of VEGF and elevated S/D ratio of umbilical artery were responsible for the changes of the resistance of placental blood flow in severe preeclampsia. Furthermore, elevated S/D ratio of umbilical artery velocity was essential as a surveillance method of fetal health status with IUGR (Intrauterine growth restriction) by vascular declination of placenta.

Cisplatin에 의한 HeLa세포의 세포고사에 미치는 활성산소종의 영향

고경희 ( Go Gyeong Hui ),김신호 ( Kim Sin Ho ),조해중 ( Jo Hae Jung ),오성환 ( O Seong Hwan ),김흥곤 ( Kim Heung Gon ),박래길 ( Park Lae Gil ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.12

목적 : 본 연구는 cisplatin이 자궁경부암세포주 HeLa세포에서 세포 고사를 유발하는 신호전달 과정에 세포내 산화제의 축적을 포함하는지의 여부를 알아보기 위하여 수행하였다. 연구 방법 : 자궁경부암 세포주 HeLa세포를 이용하여 체외 실험을 통해 항암치료중 활성산소종의 생성 여부와 항산화 물질을 세포내 투입하여 cisplatin 유래 활성 산소종에 의한 세포사멸 보호효과를 flow cytometry에 의해 측정하였다. 결과 : cisplatin에 Objective : To determine whether oxidants are formed as part of the cisplatin-induced apoptotic process, intracellular markers of oxidative stress were examined. Methods : Apoptotic death of HeLa cells by cisplatin was confirmed by flow cytometry. Results

자궁내막증 치료 전후 환자의 혈청이 생쥐 난자의 수정률에 미치는 영향

김기석,민부기,이희섭,홍기연,이선영,박현진,김흥곤,Kim, Kie-Suck,Min, Bu-Kie,Rhee, Hee-Sub,Hong, Kie-Youn,Lee, Sun-Young,Park, Heon-Jin,Kim, Heung-Gon 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.3

Objective: To evaluate the effect of serum obtained before and after treatment for endometriosis on in vitro fertilization and development of two cell mouse embryo. Design: Pretreatment and posttreatment comparoson of fertilization of mouse oocyte and embryo development in serum supplement from patients with endometriosis; result were compared using Stuent T-test analysis. Method: Infertility Clinic, Department of Obstetrics and Gynecology, Collage of Medicine, Won kwang university, Korea. Patients was chosed eleven consecutive women with endometriosis. Interventions was all patient underwent laparoscopic or conservative surgery. This was followed by a 6-month course of burserelin acetate $900{\mu}g/d$. Main outcome was measured total number of fertilization and embryo that was fertilization after 24 hours and reached blastocyst stage after 72 hours of incubation were compared before and after treatment. Result: Before treatment, 47% of the oocyte were fertilized and 31% of the embryo reached blastocyst stage. After treatment, Significantly more fertilized and Significantly more embryo developed to blastocyst on the stage I and II of endometriosis. Conclusion: The fertilization and embryo toxicity of serum samples from patients with endometriosis is lost after treatment.

자궁내 피임장치와 동반된 골반방선균증 2례

이금신(Keum Sin Lee),김훈영(Hun Young Kim),김병륜(Byung Ryun Kim),이봉주(Bong Ju Lee),김흥곤(Heung Gon Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.5

Actinomycosis is a rare cause of indolent pelvic infection secondary to the seeding of upper genital tract from other intraabdominal or hematogenous dissemination of actinomyces. In addition, ascending pelvic actinomycotic infection has been described in patients with IUD. Pelvic actinomycosis has variable clinical manifestations, which make it diffcult to diagnose preoperatively. Colonization of lower genital tract by Actinomyces occurs most often in IUD users and increases with the duration of IUD use. We have experienced two cases of actinomycosis in IUD users and report them with a review of literatures.