삼배체 괴경형성 ( 塊莖形成 ) Solanum 을 이용한 종의 구분에 관한 연구

김혜영,H . T . Erickson (Hei Young Kim) 한국식물학회 1979 Journal of Plant Biology Vol.22 No.3

Triploids of inter-and intra-specific hybrid of tuber-bearing Solanum, involving 4x S. tuberosum, S. stoloniferum, S. fendleri and S. chacoense and other diploid species were used to study the affinity between the species. The number of multivalent formations in triploids depended on the cross combination. However, multivalent formation from triploids produced by 4x×2x crosses within S. chacoense did not differ from triploids from S. tuberosum (4x)×S. chacoense (2x). Many of the triploid hybrids had 13 or more bivalents plus trivalents and quadrivalents indicating slight autosyndesis. As the basic chromosome number in potato is assumed to be 12, the slight excess pairing might be due to structural differences of chromosomes and eventually to non- or partial-homologous pairing. Male fertility of triploid hybrids was very low (less than 7.2% stainable pollen) with exceptions of triploids, involving S. tuberosum × S. chacoense (49.16%) and S. Chacoense (4x) × S. chacoense (2x) (30.25%). Pillen fertility was associated with chromosome affinity at first metaphase rather than with chromosome distribution at second metaphase. The large number of multivalents and high pollen fertility in the triploid hybrid of S. tuberosum × S. chacoense, indicates a very high affinity of these two species.

Particle Bombardment에 의해 전처리 된 참나리(Lilium lancifolium Thunb.) 캘러스의 Agrobacterium tumefaciens을 통한 형질전환

남상욱,김혜영,Nam, Sang-Wook,Kim, Hei-Young 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.1

형질전환 효율을 높이기 위하여, NPTII와 GUS유전자를 포함하고 있는 pIG121Hm을 미세 입자에 코팅하여 particle bombardment를 수행한 후 pIG121Hm가 도입된 Agrobacterium tumefaciens EHA101와 3일간 공동 배양하였다. 그 후 캘러스를 1mg/L 2,4-D, 0.1mg/L BAP, 100mg/L kanamycin, 그리고 200mg/L carbenicillin이 포함된 MS배지로 옮겨 4주간 성장시킨 후 kanamycin저항성 캘러스를 선발하여 GUS 발현을 관찰하였고, PCR 분석을 통하여 700 bp의 NPT II 유전자가 도입된 것을 확인하였다. Particle bombardment 후 Agrobacterium과 공동배양 한 처리구가 Agrobacterium과 공동배양만 한 처리구보다 GUS발현 분석에 의한 형질전환 효율이 3배정도 더 높았다 형질전환 된 재분화 식물체를 얻기위해 다시 선발된 kanamycin저항성 캘러스는 1mg/L NAA와 1mg/L BAP가 포함된 재분화 배지에 옮겨져 4주 후 형질전환 된 소인경을 형성하였다. To improve transformation efficiency, the callus of Lilium lancifolium Thunb. were bombarded by particles coated with pIG 121 Hm which include NPT II and GUS genes, and then cocultivated with Agrobacterium tumefaciens EHA101 which contain pIG121Hm binary vector, carrying neomycin phosphotransferase (NPT II) and $\beta$-Glucuronidase (GUS) genes. Three days after cocultivation with Agrobacterium tumefaciens and particle bombardment, the callus clusters were transferred to MS medium containing 1mg/L 2,4-D, 0.1mg/L BAP, 100mg/L kanamycin and 200mg/L carbenicillin. Four weeks after transfer to the selection medium, GUS expression was detected and PCR analysis revealed the presence of NPT II fragment of the expected size (700 bp) in the transformed callus. The GUS expression from Agrobacterium-mediated transformants after particle bombardment increased to over 3-folds compared with that of callus cocultivated with Agrobacterium tumefaciens without particle bombardment. The callus clusters containing kanamycin resistant gene were transferred to MS medium containing 1mg/L NAA and 1mg/L BAP. Somatic embryos were developed in four weeks and microbulbs expressing GUS were formed.

참나리 (Lilium lancifolium Thunb.) 인편으로부터 부정아 발생과 캘러스 유도를 통한 식물체 재생

남상욱,김혜영,Nam, Sang-Wook,Kim, Hei-Young 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.1

여러가지 생장조절제를 첨가한 MS배지에 참나리의 기내배양 소인편과 실생인편을 치상하여 재분화 과정을 알아보았다. 소인편의 부정아 발생은 BAP 1.0mg/L와 NAA 0.1 mg/L을 혼합 처리한 MS배지에서 4주간 배양했을 때 가장 왕성하게 일어났으며, 실생인편의 부정아 발생률은 BAP 0.5mg/L와 NAA 0.1 mg/L에서 4주간 암배양했을 때 가장 높았다. 소인편의 캘러스 발생은 BAP 0.1 mg/L와 2,4-D 1.0 mg/L 혼합 처리구에서 가장 높았다. 또한. 캘러스의 재분화는 NAA 0.5 mg/L와 BAP 0.1 mg/L를 혼합 처리한 MS배지에서 8주간 명배양했을 때 가장 높았다. 뿌리는 MS배지에서 쉽게 발달되었으며, 8주 후 굵고 긴 뿌리를 갖춘 완전한 재분화 식물체로 성장하여 화분에 이식하였다. 참나리의 재분화 전, 후의 염색체 수는 모두 2n=24로서 조직배양에 의한 염색체수의 변화가 없음을 확인하였다. This study was performed to investigate the effects of various media compositions in regeneration of Lilium lancifolium. The adventitious bud initiation from microscale was the best on MS medium supplemented with BAP 1.0 mg/L and NAA 0.1 mg/L after 4 weeks of culture. However, from bulbscales, adventitious bud initiation was the best in dark condition on MS medium supplemented with BAP 0.5 mg/L and NAA 0.1 mg/L. On the other hand, callus induction was found to be the best from the microscales incubated in complete dark condition for 8 weeks on MS medium supplemented with 2,4-D 1.0 mg/L and BAP 0.1 mg/L. The highest plantlet regeneration from callus was obtained after incubation in the light condition for 8 weeks on MS medium supplemented with NAA 0.5 mg/L and BAP 0.1 mg/L. Rooting of shoots was obtained easily on MS medium and the plantlets were transferred to soil pots after 8 weeks. The chromosome analysis of the root tip cells was revealed that the callus-derived plantlets had normal chromosome number, 2n=24. No variation was observed in the morphology of the plantlets.

한국인 여성 중 양성 또는 악성 유방 종양 조직에서 FISH 에 의해 발견된 염색체의 이수성

김진미(Jin Mi Kim),박소연(So Yeon Park),김혜영(Hei Young Kim) 한국유전학회 2001 Genes & Genomics Vol.23 No.1

N/A Several reports have described aberrations of chromosome 1, 11, and 17 in breast tumor, which harbour genes of causative importance for tumorigenesis and propagation. The occurrence of aneuploidy involving chromosomes 1, 11, and 17 in malignant and benign breast tumor tissues has not been studied systematically in Korean women. Fifteen benign and thirty malignant breast tumor tissues in Korean women were studied in interphase nuclei using fluorescence in situ hybridization (FISH) with centromere-specific probes for chromosomes 1, 11, and 17. All the benign tumor tissues showed normal diploidy patterns, except one case which was monosomic for chromosome 1. However, twenty-six of the thirty malignant breast tumor samples (86.67%) had aneuploidy at least in one of the three chromosomes tested. A significant difference was noted between malignant breast tumors and normal or benign tumor tissues. The frequency of cells with disomy and polysomy of chromosomes 1, 11, and 17 in the malignant tumors were significantly different from the normal or benign tumors in the malignant tumors were significantly different from the normal or benign tumors by student's T-test. None of the benign breast tumors were different from the normal samples in the frequencies of monosomy, disomy, and polysomy in chromosomes 1, 11, and 17 except monosomy and polysomy of the chromosome 17 by student's T-test. The frequencies of cells with aneuploidy for chromosome 1, 11, and 17 in malignant breast tumors were 90%, 53.33%, and 76.67%. Since the frequencies of aneuploidy of chromosomes 1, 11, and 17 in malignant breast tumor, examination of aneuploidy by FISH can be efficiently applied to the diagnosis of malignant breast tumors.

四倍體와 二倍體間의 種間交雜에 의한 감자 半數體 誘起

趙賢默(Cho Hyun Mook),金惠英(Kim Hei Young),睦一振(Mok Il Gin) 한국원예학회 1995 Horticulture, Environment, and Biotechnology Vol.36 No.2

감자 엽조직 원형질체로부터 식물체 재분화

안율균(Yul Kyun Ahn),김혜영(Hei Young Kim),윤진영(Jin Young Yoon),박효근(Hyo Guen Park) 한국원예학회 2001 Horticulture, Environment, and Biotechnology Vol.42 No.4

재배종 감자(Solanum tuberosum)와 야생종(S. brevidens)과의 종간 체세포잡종 식물체 생산

안율균(Yul Kyun Ahn),김혜영(Hei Young Kim),최학순(Hak Sun Choi),김기택(Ki-Taek Kim),박효근(Hyo Guen Park) 한국원예학회 2001 Horticulture, Environment, and Biotechnology Vol.42 No.4

감자의 4x×2x 종간교잡 후대 식물체의 배수성 차이에 따른 氣孔細胞의 量的形質 變化와 반수체식물 선발효과

Hyun Mook Cho(趙賢默),Hei Young Kim-Lee(金惠英),Il Gin Mok(睦一振) 한국육종학회 1994 한국육종학회지 Vol.26 No.1

To select haploids from the progeny plants obtained by interploidal and interspecific crosses of potatoes, stomatal cell characters were examined at three different growth stages as a potential indicator of ploidy level. The mean stomatal cell densities were 66.9, 45.0 and 38.5 per unit area(34 ㎛²) in haploid, triploid and tetraploid plants, respectively. The length and width of stomata were 27.5 : 18.8㎛, 34.3 : 21.0㎛ and 36.5 : 22.0㎛ in haploid, triploid and tetraploid plants, respectively. These characters had significant differences between ploidy levels with in the progeny plants but had relatively large quantitative variation within ploidy levels. On the other hand, the number of chloroplasts in a pair of guard cells, having the mean of 12.2, 18.4 and 20.2 in haploid, triploid and tetraploid plants, respectively, had large differences between ploidy levels with narrow variation within a ploidy level. These results indicated that counting chroloplasts in the guard cells among stomatal cell characters could be adopted as a rapid and accurate method for identifying haploids from large number of progeny plants of interploidal and interspecific crosses.

單位生殖에 의한 감자 半數體植物 誘起 Ⅲ. 色素遺傳子 發現樣相에 따른 半數體植物 選拔

Hyun Mook Cho(趙賢默),Hei Young Kim Lee(金惠英),Il Gin Mok(睦一振) 한국육종학회 1993 한국육종학회지 Vol.25 No.1

Interspecific and Interploidal crosses were made between tetraploids and diploids to induce potato haploids. Tetraploids female parents were grouped into three different types according to the pigments on the stem. Pigments on seeds, seedlings and mature plants were used as phenotyphic markers to select haploids. Haploids were derived only from non-embryo spot seeds from all of the three types of the female parents. The positive selection for non-pigmented plants at seed and seedling stage was highly accurate to select haploid in the progenies of the female parents with green stem (group G), except the cv. Russet Burbank. Most of seedlings with white hypocotyl in the progenies of female parents with basal purple(group BP) or whole purple stem(group P), were haploids. However, 45.5% and 69.5% of the seedlings with white-purple hypocotyl derived from these groups were haploids, respectively. Because of the matroclinous effect of their female parents on plant pigmentation, the negative selection for purple pigmented plants at seed, seedling, growth and subsequently, harvest stage, was effective. As a consequence, a two step selection method was applicable for group BP and P ; select the seedlings with only white hypocotyl at seedling stage as haploids, and discard all the plants with purple pigments on the hypocotyl, stem and tuber skin according to gradual plant growth. Up to now, from 1,253 of non-embryo spot seeds, 199 haploid plants were confirmed by the phenotypic markers and subsequently root-tip chromosome counting. The efficiency of haploid induction through interspecific and interploidal crosses was affected by different tetraploids female parents.

裁培種 감자 半數體와 近緣野生種間 交雜에서 endosperm balance number(EBN)의 영향

조현묵(Hyun Mook Cho),김혜영(Hei Young Kim-Lee),엄영현(Young Hyun Om),김정간(Jung Kan Kim) 한국육종학회 1997 한국육종학회지 Vol.29 No.2

The effect of Endosperm Balance Number (EBN) in interploidal and interspecific crosses between S. tuberosum dihaploids and tuber-bearing wild species was investigated. The crossability was evaluated by the percentage of berry set and the average number of seeds per berry. The percentages of berry set were 11.5%, 11.4%, 7.4% and 2.2% in 4x×4x, 4x×2x, 2x×2x and 2x×4x crosses, respectively. The number of seeds per berry was in the same order as the percentages of berry set. The crossability was greatly affected by the ratio of the maternal and parternal EBN in the endosperm. The crosses with the maternal and parternal EBN ratio of 1 : 1, 1 : 1/2 and 4 : 1 in the developing endosperm did not produce hybrid seeds. However, a lot of plump hybrid seeds were produced only in 2 : 1 of EBN ratio irrespective of differences of the species used or ploidy levels of the parents.