골밀도와 수냉증과의 상관성 연구

이동녕 ( Dong Nyung Lee ),이정임 ( Jeong Im Lee ),고호연 ( Ho Yeon Go ),김형준 ( Hyung Jun Kim ) 대한한방부인과학회 2015 大韓韓方婦人科學會誌 Vol.28 No.4

Objectives: The purpose of this study was to examine the relationship between cold hypersensitivity and bone mineral density. Methods: 28 outpatients who visited○○University Oriental Hospital from July 11th, 2013 to August 28th, 2013 were analyzed. Patients were subjected to thermometer, and those with thermal difference greater than 0.3 ℃ between upper arm and palm and also with more than VAS 4 of cold hypersensitivity were diagnosed with cold sensitivity. Lumba spine (L2-4) and heel bone measurement were obtained on dual energy X-ray absorptiometry (DEXA) and Quantitative Computed Tomography (QCT). Results: 1. There was statistically significant correlation between T-score of DEXA and body weight (0.522), BMI (0.643), OSTA (Osteoporosis elf-assesment Tool for Asians, 0.579), BMI, T-score and Z-score of QCT. OSTA and T-score of QCT (0.521) also had statistically significance. 2. There was no statistically significant correlation between body fat, abdominal obesity rate, degree of cold hypersensitivity, upper arm and palm’s temperature difference, palm temperature, height and Z-score, T-score of QCT or T-sore of DEXA. Conclusions: Bone mineral density was lower in women who had cold hypersensitivity of hand and feet. But there was no statistically significant correlation between bone mineral density and degree of cold hypersensitivity, upper arm and palm’s temperature difference.

한의표준임상진료지침 개발을 위한 수족냉증에 대한 한의사의 인식과 치료현황

이동녕 ( Dong-nyung Lee ),김형준 ( Hyung-jun Kim ),유준상 ( Jun-sang Yu ) 대한한방부인과학회 2017 大韓韓方婦人科學會誌 Vol.30 No.3

Objectives: The purpose of this study were to researched a Korean medicine doctors` recognition about coldness of hands and feet, and developing of korean medicine clinical practice guidelines (CPG) for coldness of hands and feet. Methods: We conducted a questionnaire survey targeting 399 Korean medicine doctors belonging to the Association of Korean Medicine by e-mail and analyzed the answers. Results: 1. 86.86% of the respondents agreed about the necessity of CPG for coldness of hands and feet. 2. 84.2% of respondents wanted coding of Korean Standard Classification of Diseases (KCD) on coldness of hands and feet. 3. To diagnosis a coldness of hands and feet, the respondents used a Subjective symptoms (98.5%), Infrared thermographic imaging device (DITI) (26.32%) Heart rate variablity test (HRV) (17.04%), Thermometer (9.77%), Cold stress test (2.76%) 4. Causing of coldness of hands and feet, the respondents considered a constitution or heredity (84.71%), stress (73.66%), lack of exercise (64.91%), irregular eating habits (51.63%), Cold meals (32.83%), depression (31.33%), etc. 5. Treating coldness of hands and feet, the respondents used a herbal medicine (66.85%), acupuncture (70.7%) Pharmacopuncture (23.85%) and moxibustion (60.08%) for 10.91±8.03 week. Conclusions: We researched a Korean Medicine doctors` recognition of CPG, clinical diagnosis, treatment on a coldness of hands and feet, and policy they required.

Gardnerella vaginalis에 대한 목단피, 소목, 어성초, 연교, 하고초, 현삼의 항균효과

장경호,이동녕,김형준,Zhang, Kyung-Ho,Lee, Dong-Nyung,Kim, Hyung-Jun 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3

The purpose of this thesis is to dedicate to get the objectivity of herbal medicine treatments by choosing herbs likely to work as antibacterial agents, Moutan Cortex Radicis, Caesalpiniae Lignum, Houttuyniae Herba, Forsythiae Fructus, Prunellae Herba, Scrophulariae Radix against Gardnerella vaginalis which is associated with Bacterial vaginosis, making experiments on them and getting the significant results. Each herb's efficacy on control the number of Bacterial vaginosis is noticed by using Disk Susceptibility test with six herbs medicine and Broth dilution assay of the culture. Disk Suseptibility Test : The efficiency strength is as follows in a row : Caesaipiniae Lignum, Moutan Cortex Radicis, Prunellae Herba, Forsythiae Fructus, Houttuyniae Herba, Scrophulariae Radix. MIC Prunellae Herba is 1.5 mg/ml, Caesalpiniae Lignum is 5 mg/ml, Forsythiae Fructus and Moutan Corex Radicis is 10mg/ml for G. vaginalis. As a results, herbmed which tested in this study have an antibacterial effects against G. vaginalis and that may be used for treament of Bacterial vaginosis. Assistant use of external medicine to p.o medicine is expected to bring the good effect of treatment which is used for the origin of this disease.

온경탕이 족부냉증에 미치는 영향에 대한 다기관 이중맹검 무작위 대조군 연구

권나연,유준상,김형준,이동녕,Kwon, Na-Yoen,Yoo, Joon-Sang,Kim, Hyeong-Jun,Lee, Dong Nyung 대한한방부인과학회 2020 大韓韓方婦人科學會誌 Vol.33 No.3

Objectives: This study was aimed to figure out the effect of Onkyung-tang (OKT) on cold hypersensitivity on feet in female patients. Methods: This study was designed to implement multi-center randomized clinical trial in three korean medical hospitals. The female patients who were enrolled in this study were 112 women who suffered from cold hypersensitivity on feet. They were randomly allocated into OKT group or control group in 1:1 ratio. The primary outcome was change in score of visual analogue scale of hypersensitivity on feet, and the secondary outcomes were thermometer of LU4, PC8, ST32, LR3, visual analogue scale of hypersensitivity on hands, result of cold stress test, and score of World Health Organization Quality of Life-BREF. Results: In both group, the VAS of hypersensitivity on feet and hands was decreased while taking the medicine, and the effect of decreasing the VAS continues for the 4 weeks after the end of medication (p<0.001, 0.003, 0.001). In OKT group, the body temperature difference in lower extremity (ST32-LR3) was steadily decreased at every visit and it was statistically significant(p=0.003, <0.001). On the other hand, in control group, the body temperature difference in lower extremity was decreased only while taking the medicine (p<0.001). Conclusions: OKT significantly reduced discomfort coldness on feet in patients, and the effect of treatment was maintained at least for four weeks after medication.

과루인이 자궁경부암세포의 성장억제 및 세포고사에 미치는 영향

이정구,김연희,이동녕,김형준,Lee Jeong Gu,Kim Yeon Hee,Lee Dong Nyung,Kim Hyung Jun 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3

To investigate the effects of Trichosanthes semen extract on the growth and apoptosis of human uterine cervical carcinoma cells. Effects of Trichosanthes semen extract on the growth of ME-180 cells were assayed by MTT assay. Apoptosis induced by Trichosanthes semen extract was detected by fluorescent microscopy, DNA fragmentation analysis and flow cytometry. Caspase-3 and caspase-8 activities were assayed. Trichosanthes semen extract induced ME-180 cells to die in a dose- and time-dependent manner. ME-180 cells treated with Trichosanthes semen extract exhibited typical characteristics of apoptosis. The population of Sub-G1 cells increased significantly, and the cells represented the reduced size, condensed chromatin and apoptotic bodies. They showed the decreased mitochondrial membrane potential and increased activities of caspase-3 and caspase-8. The results suggest that Trichosanthes semen extract induced ME-180 cell apoptosis and the activation of caspase and mitochondrial pathway were involved in the process of Trichosanthes semen extract-induced apoptosis.

수족냉증에 대한 전침치료의 사상체질별 효과 및 안전성: 다기관 무작위 대조군 연구

권나연,유준상,김동일,김형준,이동녕,Kwon, Na-Yoen,Yu, Jun-Sang,Kim, Dong-Il,Kim, Hyeong-Jun,Lee, Dong-Nyung 사상체질의학회 2021 사상체질의학회지 Vol.33 No.2

Objectives The purpose of this study was to figure out the effect and safety of electro-acupuncture and acupuncture on cold hypersensitivity on hands and feet(CHHF) by Sasang constitution. Methods 72 subjects were recruited from three Korean medical hospitals from October 2019 to October 2020. Subjects were randomly allocated in 1:1:1 ratio to electro-acupuncture group(EA group), acupuncture group(AC group), and control group. The EA and AC group received treatment twice a week for 5 weeks. We tried to confirm the effectiveness of electro-acupuncture and acupuncture treatment by checking changes in Visual analogue score(VAS), body temperature of LU4, PC8, ST32, LR3, and WHOQOL-BREF score. Results The VAS score of CHHF was reduced after treatment, and the effect continued for 4 weeks after the end of treatment in all Sasang constitution, all treatment groups. In all Sasang constitution, the body temperature of the electro-acupuncture group or acupuncture grouop was higher than that of the control group except LU4 in visit 11. And no clinically significant adverse events have been identified. Conclusions Electro-acupuncture and acupuncture treatment are effective on hypersensitivity on hands on feet, and the effect was shown regardless of Sasang constitution.

보중익기탕(補中益氣湯)이 실험적으로 유발된 흰쥐의 자궁내막증(子宮內膜症) 발달에 미치는 영향

이정구 ( Jeong Gu Lee ),이동녕 ( Dong Nyung Lee ),서일복 ( Il Bock Seo ),김형준 ( Hyung Jun Kim ) 대한한방부인과학회 2009 大韓韓方婦人科學會誌 Vol.22 No.3

紫河車(자하거) 藥鍼(약침)이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향

유영기 ( Yung Ki Yoo ),김형준 ( Hyung Jun Kim ),신미란 ( Mi Ran Sin ),이동녕 ( Dong Nyung Lee ) 대한한방부인과학회 2014 大韓韓方婦人科學會誌 Vol.27 No.1

This study was performed to investigate the effects of Hominis Placentapharmacopuncture (HPP) therapy on the experimentally-induced endometriosis in the rats. Materials and Methods: Endometriosis was induced in rats by autotransplantinguterine tissue to the peritoneum and divided them into three groups: (1) sham-operatedgroup (n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3) surgically induced endometriosis and HPP treated group. Sham-operatedgroup and control group were inject with normal saline once a every other day for30days, while treated group was injected with HPP extract once a every other dayfor same duration. Injected point of HPP and normal saline were subcutaneous tissueat Gwanwon (CV4) acupoint. Then we measured the body weight, the volume ofendometriotic implants, the weigh of uterus and ovaries, and investigated theconcentration of cytokines (MCP-1, TNF-α) in peritoneal fluids. Histopathology, immunohistochemisty for COX-2 and VEGF, and histochemistry for mast cell intransplanted uterine tissue were performed. Results: The volume (mm2) of endometriotic implants in HPP treated group(55.4±41.6) was significantly decreased (p<0.01) compared with control group (140±66.1). And the concentration (pg/ml) of MCP-1 in peritoneal fluids in HPP treated group(1117.6±60.5) was significantly decreased (p<0.01) compared with control group(1446.2±280.3). The concentration (pg/ml) of TNF-α in peritoneal fluids in HPP treatedgroup (80.6±31.4) was decreased (p<0.01) compared with control group (145.3±86.9). Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatorycell and angiogenesis in transplanted uterine tissue of HPP treated group wereweakly observed than those of control group. The COX-2 expression in endometrial, epithelial and stromal cells in transplanted uterine tissue of HPP treated group wasdecreased compared with control group. The VEGF expression of endometriotic epithelia, neovascular endothelia and stromal cell in transplanted uterine tissue of HPP treatedgroup were weakly observed than those of control goup. Conclusions: HPP is effect on Endometriosis of rats by Experimentally-induced.

瓜蔞仁이 자궁경부암세포의 성장억제 및 세포고사에 미치는 영향

이정구(Jeong Gu Lee),김연희(Yeon Hee Kim),이동녕(Dong Nyung Lee),김형준(Hyung Jun Kim) 한의병리학회 2005 동의생리병리학회지 Vol.19 No.4

혈부축어탕(血府逐瘀湯)이 Estradiol Valerate로 유발된 흰쥐의 다낭성 난소 및 수태에 미치는 영향

배상진 ( Sang Jin Bae ),김형준 ( Hyung Jun Kim ),이동녕 ( Dong Nyung Lee ) 대한한방부인과학회 2012 大韓韓方婦人科學會誌 Vol.25 No.2

Objectives: This study was designed to investigate the effects of Hyeolbuchukeotang( HCT) on the Estradiol Valerate(EV) induced Polycystic Ovaries and the Implantation of rats. Methods: PCO was induced by single intramuscular(IM) injection with EV in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for 60 days. Control group(n=8) were injected with EV and orally administrated distilled water for 60 days. HCT treated group(n=8) were injected with EV and orally administrated HCT for 60 days. At the end day of experiment, we measured weights of body, ovaries, adrenal glands, uterus and contents of serum LH, FSH, ADD. The histomorphometrical changes of ovaries were also evaluated. And we observed the NGF and CRF expression by immunohistochemistry. Also we observed the mating and count the number of implantation. Results: - The weights(㎎) of ovaries in HCT treated group(58.9±6.5) were significantly increased (p<0.01) compared with control group (42.3±8.5). - The number of mature follicles in HCT treated group(9.8±2.6) was significantly increased (p<0.01) compared with control group(6.1±2.1). - The number of cystic follicles in HCT treated group(1.6±1.2) was significantly decreased (p<0.01) compared with control group(3.8±1.5). - The value of serum FSH(mIU/㎖) in HCT treated group(3.870±2.151) was significantly decreased(p<0.05) compared with control group(10.476± 6.294). - The expression of NGF-immunoreactive cells in the ovarian cells in HCT treated group was lesser observed than control group. - The number of implantation in HCT treated group(7.4±1.8) was significantly increased (p<0.05) compared with control group(3.1±4.4). Conclusions: Hyeolbuchukeo-tang(HCT) is effect on polycystic ovaries and the implantation of rats by EV-induced.