Saccharomyces cerevisiae에서 Cycloinulooligosaccharide Fructanotransferase 유전자의 표층 발현

김현진,이재형,김현철,김연희,권현주,남수완,Kim, Hyun-Jin,Lee, Jae-Hyung,Kim, Hyun-Chul,Kim, Yeon-Hee,Kwon, Hyun-Ju,Nam, Soo-Wan 한국생명과학회 2007 생명과학회지 Vol.17 No.2

Paenibacillus macerans 유래의 cycloinulooligosaccharide fructanotransferase (CFTase) 유전자(cft, 2832 bp, 103.8 kDa)를 효모 표층발현 vector인 pCTcon (GAL1 promoter)에 subcloning 하였다. 구축된 pCTECFTN (9.0 kb)를 숙주세포인 s. cerevisiae EBY100에 형질전환한 후, uracil이 결핍된 배지와 inulin 함유배지에서 선별하였다. cft 유전자는 GAL1 promoter에 의해 효모 형질전환체에서 성공적으로 발현되었다. inulin으로부터 cyclofructans (CFs)로 생산하는 효소적 능력으로부터 표층발현 유무를 확인하였다. YPDG배지에서 48시간 배양 후 분획한 균체는 5.52 unit/ l 의 활성을 보였다. CF 생산을 위한 효소의 최적 반응 조건으로 pH 8.0, 반응온도 $50^{\circ}C$, 기질농도 5%, 기질은 Jerusalem artichoke 등의 inulin과 표층 발현 CFTase 효소반응 결과, cycloinulohexaose (CF6), cycloinuloheptaose (CF7), 그리고 cycloinulooctaose (CF8)이 생성되었고, 이 중에서 CF6가 주 생성물이었다. The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTECFTN (9.0 kb) was introduced to S. cerevisiae EBY100 cell and then east transformants were selected on the synthetic defined medium lacking uracil and on the inulin containing medium. The surface display of CFTase was confirmed by immunofluorescence microscopy and its enzymatic ability to form cycloinulooligosaccharides(cyclofructans, CFs) from inulin. The total activity of the CFTase was reached about 5.52 unit/1 by cultivation of yeast transformant on YPDG medium. The optimized conditions determined were as follows; pH, 8.0; temperature, $50^{\circ}C$ ; substrate concentration, 5%; inulin source, Jerusalem artichoke. By the reaction with inulin, CFs consisting of cycloinulohexaose (CF6), cycloinuloheptaose (CF7), and cycloinulooctaose (CF8) were produced and CF6 was the major product.

Burden of Disease Due to Outdoor Air Pollution in Korea: Based on PM₁₀

김현진,윤석준,김형수,이건세,김은정,조민우,오인환,Kim, Hyun-Jin,Yoon, Seok-Jun,Kim, Hyeong-Su,Lee, Kun-Sei,Kim, Eun-Jung,Jo, Min-Woo,Oh, In-Hwan Korean Society of Environmental Health 2011 한국환경보건학회지 Vol.37 No.5

Purpose: This study measured the burden of disease in Korea related to outdoor air pollution using disability-adjusted life year (DALY). Materials and Methods: As a risk factor of outdoor air pollution, particulate matter with a diameter less than 10 ${\mu}m$($PM_{10}$) was used. First, $PM_{10}$-related diseases and their relative risk (RR) were selected by means of a literature review. Second, population attributable fractions were computed by using formulae including RR and population exposure to $PM_{10}$. Third, DALYs of $PM_{10}$-related diseases in Korea were estimated. Finally, the attributable burden of disease due to $PM_{10}$ was measured as the sum of the products that multiplied the DALYs of $PM_{10}$-related diseases by their population attributable fractions. Results: The disease burden of PM10 was 6.9 DALY per 1,000 persons in 2007. The attributable burden of $PM_{10}$ was 2.68 for lung cancer, 2.41 for COPD, 0.62 for ischemic heart disease, 0.61 for pneumonia, 0.55 for asthma, and 0.03 for preterm. Conclusions: This study showed the environmental burden of disease of $PM_{10}$ and burden of $PM_{10}$-related disease through objective data. It also suggested that active efforts are needed to continuously measure and reduce the burden of environmental diseases in Korea.

음파 가진을 이용한 2차원 웨이크 유동 제어에 관한 연구

김현진,김재호,김명균,Kim, Hyeon-Jin,Kim, Jae-Ho,Kim, Myeong-Gyun 대한기계학회 1998 大韓機械學會論文集B Vol.22 No.6

In a low speed open-type wind tunnel, a group of parallel wakes downstream of two dimensional grid model consisting of several circular cylinders were experimentally investigated to study the response of the wake flows to the acoustic excitation, in hoping to promote the understanding of the underlying mechanism behind the gross flow change due to artificial excitation. In the unexcited wake flows, the development of the individual wakes behind cylinders was almost uniform for the ratio of the spacing to the cylinder diameter of s/d.geq.1.5. For smaller s/d, however, the jet streams issued through the gaps between the cylinders became biased in one side and the cylinders had wakes of different sizes. At s/d=1.25, the gap flow directions change in time, leading to unstable wake patterns. Further reduction in s/d made this unstable flip-flopping of the jets stable. The most effective excitation frequency was found to be in the Strouhal number range of St=0.5-0.6. This frequency was related to the vortex shedding. At s/d=1.75, the excitation frequency was 2 or 4 times the vortex shedding frequency. When the flow was excited at this frequency, the vortex sheddings were energized, and pairings between neighboring vortices were generated. Also, the merging process between individual wakes was accelerated. The unstable and unbalanced wake patterns at s/d=2.15 were made stable and balanced. The unstable and unbalanced wake patterns at s/d=2.15 were made stable and balanced. For smaller spacing of s/d .leq,1.0, the acoustic excitation became less effective in controlling the flow.

Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands

정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3

침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.

고령자 그룹홈의 운영과 거주자의 생활 실태

김현진,안옥희,Kim Hyun-Jin,An Ok-Hee 한국주거학회 2006 한국주거학회 논문집 Vol.17 No.4

To investigate the actual management condition of group homes for the elderly and their living environment, four group homes and 26 elderly people living in the group home were surveyed. As the results, the relationships between the administrator and the elderly were acceptable, but the group homes seem suffer from insufficient administrative supports. The most required service in the group homes was a conversational partner service. Moreover, the most satisfactory point for the elderly living in group homes was the settlement of food, clothing, and housing. The unsatisfactory things were the service related to medical, leisure, and house work and an invasion of their private life. The resident elderly in group homes were fully satisfied with their living environment including the relationship with the manager and the people in the facility. They, however, do not have much chance to make an exchange with their neighbor. Overall, the elderly want to stay continuously in the group homes where they are living current.

고유가 정착 및 교토의 정서 발효가 기업에 미치는 영향 및 대응

김현진,Kim, Hyeon-Jin 대한석유협회 2005 석유와 에너지 Vol.2005 No.3

스트레스 완화 목적의 아로마 요법에 관한 문헌고찰

김현진,정수현,정혜인,김경한,Kim, Hyeon-Jin,Jeong, Soo-Hyun,Jeong, Hye-In,Kim, Kyeong Han 대한예방한의학회 2021 대한예방한의학회지 Vol.25 No.2

Objective : This study was aimed to review randomized controlled trials (RCTs) about whether aromatherapy relieves stress. Method : We searched document about criteria to use words like 'Aroma', 'Oil' and 'Stress'. The study included 24 RCTs which were selected by total 167 studies searched in Korean Journal by searching OASIS, ScienceON, KISS, RISS. Cases that cannot be performed alone are excluded. Results : We got 24 domestic standard documents. Of the 24 studies, 14 were for students, and 6 were for patients receiving hospital treatment. Among the 7 treatments, dry-inhalation was used 13 times, and necklace-inhalation was used 9 times. Of the 24 Studies, lavender oil was used 19 times and sweet orange was used 4 times. Among the 28 types of measuring instruments used, 10 related to the autonomic nervous system and 8 STAIs and VASs were used respectively. Conclusion : It was possible to conclude that aromatherapy was effective in relieving stress. Through further research, it is necessary to study effective oil mixing methods, methods for measuring subjective stress, multimodal intervention, and effective intervention periods.

독일 기록관리 담론에서의 평가론

김현진,Kim, Hyun-Jin 한국기록학회 2006 기록학연구 Vol.0 No.14

이 글은 독일 기록관리 이론의 역사를 평가론 중심으로 개괄한다. 18세기 이전 독일의 기록관리의 목표는 법률적, 정치적 지식을 조직하는 것이었다. 따라서 기록 소유자의 법률적 권리보전을 위해 특별히 중요한 증명서나, 정치적으로 중요한 문서들만 선별, 관리되었다. 19세기에, 구체제의 붕괴로 보호해야 할 특권이 없어지면서 기록관리의 목적은 연구기능을 지향하게 되었다. 다양한 기관에서 생산되어 집적된 기록들을 조직하기 위해 출처주의가 확립되었고, 학문적인 필요에 부합하는 내용지향적인 평가방식이 선호되었다. 1차 대전은 현대 기록학 이론 형성의 결정적인 계기가 된다. 전쟁수행 중 생산된 방대한 양의 기록을 처리해야 하는 당면 과제가 종전 이후의 민주화 분위기와 결합하여 출처 지향적 평가론이 형성된다. 기록의 가치는 생산기관의 업무와 활동을 증거하는 능력에 따라 평가되는 것이다. 나찌 시대를 지나 온 전후 독일의 반(反)관료제적인 분위기 속에서 출처 지향적 평가론 대신 내용지향적인 평가방식이 다시 등장한다. 봄스로 대표되는 이 이론은 20세기 후반 다큐멘테이션 전략 이론의 토대가 된다. In diesem Aufsatz wird die Geschichte der Bewertungsdiskussion in Deutschland vorgestellt. Bis zum 18. Jh. war das Ziel der Archivarbeit die Systematisierung der rechtlichen und politischen Kenntnissen. Der juristisch geschulte Archivar hob Unterlagen in der Regel aus $Gr{\ddot{u}}nden$ der Rechtssicherheit auf. Vernichtet wurde, was schon seit $l{\ddot{a}}ngerem$ im Archiv lag. Nach dem Zusammenfall des alten Regimes haben die Archivare keine mehr mit dem Recht des Herrschers zu tun. Und die Archive wurden zu $Lagerst{\ddot{a}}tten$ der Geschichte und Forschung. $F{\ddot{u}}r$ den Ordnung der Archivgut, die aus den verschiedener Dienststellen stammten, wurde das Provenienzprinzip gebildet. Die Archivare richtete sich auf das inhaltsorientierten Bewertungsverfahren, das die wissenschaftlichen $Bed{\ddot{u}}rfnisse$ befriedigte. Der Anlass der Bildung der modernen Archivtheorie war der ersten Weltkrieg. Das Problem, wie man die Massenakten, die $W{\ddot{a}}hrend$ dieses Krieges entstanden, behandeln sollten, $f{\ddot{u}}hrte$ unter dem $Einflu{\ss}$ der Demokratie zur Bildung der Provenienz-orientierte Bewertungsdiskussion. Aufbau, Zielsetzung und Verfahren der aktenbildenden Stelle bildeten den Ausgang der Bewertung. Im Klima ${\ddot{o}}ffentlicher$ $B{\ddot{u}}rokratieablehnung$ der 50er und 60er Jahren wurde die inhaltsorientierte Auswahl wieder die vorherrschende Methodenvorgabe. Diese Theorie, die Booms vertritt, $f{\ddot{u}}hrt$ zu der Theorie der dokumentation strategy von Ende 20. Jh.

농촌노인 여가복지시설 이용 실태 및 만족도에 관한 연구 - 의성군 지역을 중심으로 -

김현진,김용범,Kim, Hyun-Jin,Kim, Yong-Bum 한국주거학회 2010 한국주거학회 논문집 Vol.21 No.5

Although the government financial aid is insufficient and the lack of programs and facilities prevent it from developing and activation in the rural local society, welfare centers for the aged are formed an ever-greater part of the leisure and welfare facility for the aged in rural areas. As a result, the objectives of this study are to investigate and identify the actual conditions of use by the aged and the degree of satisfaction for welfare centers for the aged in Uiseong district. The results of this study are as follows. In the survey sample characteristics based on a total of 303 survey, the percentage of women (55.2%) was much higher than men (44.8%), average age is 73.3 years old. According to the results of the analysis, most important motivation is for health and making friends, and major useful facilities and programs are related to physical activities, sing a song and rehabilitation. From the results of the overall satisfaction, positive promotions and sufficient aid are required for the aged in the rural local society. Further any programs developed internally are required to promote and activate the leisure activity programs for the aged.

감염인 커뮤니티의 현재와 미래대응 방향

김현진,Kim, Hyeon-Jin 대한에이즈예방협회 2007 레드리본 Vol.76 No.-