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치주인대세포와 치은섬유아세포의 분화에 미치는 rhBMP-2의 효과에 대한 연구
김현종,최상묵,구영,류인철,정종평,한수부,이용무,Kim, Hyeon-Jong,Choi, Sang-Mook,Ku, Young,Rhyu, In-Chul,Chung, Chong-Pyoung,Han, Soo-Boo,Lee, Yong-Moo 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.2
BMP can induce ectopic bone formation when implanted into sites such as rat muscle and can greatly enhance healing of bony defects when applied exogenously. In addition, BMP stimulated osteoblastic differentiation in vitro in various types of cells. The aim of this study was to investigate the effect of recombinant human bone morphogenetic protein(rhBMP-2) on the proliferation and osteoblastic differentiation of human periodontal ligament cells and gingival fibroblasts. The cell number and alkaline phosphatase activity were measured in 3 experimental groups of human periodontal ligament cells and gingival fibroblasts (control group, rhBMP-2 50ng/ml group, and rhBMP-2 100ng/ml group) at 1 and 2 weeks after culture. At the same time, total RNA of cultured cells were extracted and reverse trascription polymerase chain reaction(RT-PCR) was performed to determine the expression of mRNA of bone matrix protein. RhBMP-2 had no effect on the cell proliferation of human periodontal ligament cells and gingival fibroblasts. Alkaline phosphatase activity was elevated significantly by rhBMP-2 in both cells. And periodontal ligament cells showed significantly higher alkaline phosphatase activity than gingival fibroblasts. ${\beta}$-actin, type I collagen, alkaline phosphatase, BMP-2 mRNA were expressed in all of the samples. Osteopontin, osteocalcin mRNA were expressed in all periodontal ligament cell groups, and rhBMP-2 50ng/ml group, rhBMP-2 100ng/ml group of 2 week culture period of gingival fibroblasts. Bone sialoprotein mRNA was only expressed in rhBMP-2 50ng/ml group and rhBMP-2 100ng/ml group of 2-week culture period. These results suggest that rhBMP-2 stimulates osteoblastic differentiation in human periodontal ligament cells and gingival fibroblasts in vitro.
HTML을 지원하는 라이브러리를 이용한 웹 문서 생성 시스템의 설계 및 구현
김현종(Hyeon jong Kim),이기호(Ki Ho Lee) 한국정보과학회 1997 정보과학회 컴퓨팅의 실제 논문지 Vol.3 No.4
본 논문은 동적인 웹 문서 생성을 위해 프로그래밍 언어 Python에 HTML을 지원하는 라이브러리를 제공하고, 웹 브라우저 상에서 그래픽 사용자 인터페이스를 통해 HTML을 지원하는 라이브러리 내에 정의된 함수를 직접적으로 호출하여 웹 문서를 편리하게 생성할 수 있는 시스템을 설계 및 구현하였다. HTML을 지원하는 라이브러리는 HTML 문장을 객체화하여 웹 문서의 생성에 유용한 모듈로서 웹 문서 생성시 코드의 재사용으로 프로그램의 길이를 단축시키고 정확한 HTML 문장을 생성하도록 하며, 비순차적으로 웹 문서를 구성하게 하여 대형 문서의 작성뿐 아니라 변경 및 확장 등 웹 정보의 유지보수를 용이하게 한다. In this paper, I add a HTML-supporting library to an object-oriented programming language, Python, and implement the web document creating system. This system makes a user to be able to create a web document by calling the functions defined in the library with GUI on a web browser. The most common approach to make a CGI program is executing a sequence of print statements that create the HTML documents one line at a time. This approach makes the program longer and makes it difficult to modify and extend a web document. A HTML-supporting library is a module useful for generating web documents matching object-oriented design and supports nonsequential construction of HTML document. By using this module, users can generate a compact program and the HTML produced is correct. Documents constructed by this technique is easy to maintain as well as modify and extend.
교원섬유 기질을 이용한 3차원 세포 배양 모델에 기계적인 힘을 가하는 경우 기질을 통하여 세포에 가해지는 힘의 특성에 대한 비교 분석 연구
김현종(Hyeon-Jong Kim),류인철(In-Chul Rhyu),박준우(Jun-Woo Park),김성곤(Seong-Gon Kim) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.34 No.2
Different kinds of forces can be applied to the biological tissue. The analysis of the applied force is highly important to explain the mechanism of cellular response. In this study, the applied force to the collagen gel was analyzed by the finite elements analysis. The model received two different kinds of static force (compression and tension). The force range was 50g to 400g. In results, von Mises stress was concentrated in the peripheral region in the compression model. It was concentrated in the central area in the tension model. However, the compressive force was high in the peripheral area of the compression model and the tensional force was also high in the same area of the tension model. In conclusion, the applied force could be different to the region and it should be considered in the experiment to analyze the effects of the mechanical force on the cells.
BcN에서 IMS기반 멀티미디어 서비스의 호 처리 성능 분석
이동현(Dong Hyeon Lee),김현종(Hyun Jong Kim),최성곤(Seong Gon Choi) 대한전자공학회 2008 電子工學會論文誌-CI (Computer and Information) Vol.45 No.5
본 논문은 BcN(Broadband convergence Network)에서 IMS(IP Multimedia Subsystem)기반 멀티미디어 서비스를 제공함에 있어 세션 제어를 담당하는 CSCF(Call Session Control Function)의 호 처리 성능을 분석한 것이다. 지금까지 SIP기반의 음성 서비스의 호/세션 관련된 성능분석은 연구되고 있으나 IMS기반 멀티미디어 서비스의 호 처리 성능 분석에 대한 연구는 미흡한 실정이다. 본 논문에서는 M/M/1을 적용한 큐잉 모델과 OPNET 시뮬레이션을 통해 CSCF의 처리 용량과 가입자 수를 변화시키면서 IMS의 세션 설정 절차에 따른 호 설정 지연 시간을 측정하였다. 그 결과 총 사용자의 세션 설정 요구율이 CSCF 처리 용량의 13%이상일 경우에는 급격한 지연 증가가 나타남을 알 수 있었다. 따라서 본 성능 분석을 통해 세션 설정 지연 허용값에 맞는 CSCF의 용량과 가능한 사용자 수를 계산할 수 있을 것이다. In this paper, the call setup performance of the CSCF(Call Session Control Function) that manages the session control in providing the IMS(IP Multimedia subsystem)-based multimedia service in the BcN(Broadband convergence Network) is analyzed. While the performance related with the call/session of the SIP-Based voice service is analysed, the study for the call processing performance of the IMS-based multimedia service is insufficient. In this paper, as, the processing capacity and subscriber number of CSCF were changed, the call setup delay time according to the session setup procedure of IMS was measured using the M/M/1 queuing model and OPNET simulation. The experimental results show that the sudden increased delay showed up in case the session establishment requirement ratio of total users over 13% of the CSCF processing capacity. Therefore, the user number and capacity of CSCF suitable for the session establishment delay threshold can be calculated or estimated.