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        • KCI등재

          산화네오디뮴 기도투여에 따른 폐내 활성산소종 발생 및 DNA의 산화적 손상

          김종규 ( Jong Kyu Kim ), 김수진 ( Soo Jin Kim ), 강민구 ( Min Gu Kang ), 송세욱 ( Se Wook Song ) 한국산업위생학회 2014 한국산업보건학회지 Vol.24 No.3

          Objectives: This study was performed to assay the effect of neodymium oxide on the generation of reactive oxygen species and DNA oxidative damage by intratracheal instillation. Methods: Two groups of rats were exposed to neodymium oxide (Nd2O3) via intratracheal instillation with doses of 0.5 mg and 2.0 mg, respectively. At two days and at 12 weeks after exposure, the contents of neodymium oxide in the lung, liver, kidney, heart and brain, leukocyte, olive tail moment, ROS, RNS, lactate dehydrogenase, albumin, cytokine and MDA from BALF were measured. Results: Neodymium oxide contents in the liver, kidney, heart, and brain were detected at less than 1 μg/g tissue concentration. However, in the lungs at four weeks the highest amount were detected and then found to be drastically reduced at 12 weeks. ROS and RNS in bronchoalveolar lavage increased in concentration dependently at two days, four weeks and 12 weeks after neodymium oxide instillation. However, ROS and RNS decreased with the passage of time. At two days the total number of WBC in BALF in the high concentration group was significantly increased, and at four weeks the total number of WBC were significantly increased in the low and high concentration groups (p<0.01). At two days after exposure, the LDH of the low and high concentration groups was significantly increased. At 12 weeks, only the LDH of the high concentration group was significantly increased compared to in the control group (p<0.01). As a result of Comet assay, after two days, damage to the DNA of the low and high concentration groups was observed. Conclusions: Intratracheal instillation of neodymium oxide induces the generation of ROS and DNA damage in rats.

        • KCI등재
        • KCI등재

          은나노입자에 대한 방진마스크 포집효율 및 총누설율

          김종규 ( Jong-kyu Kim ) 한국산업보건학회 (구 한국산업위생학회) 2016 한국산업보건학회지 Vol.26 No.3

          Objectives: The production and use of nanoparticles have been increased. In 2014 Workplace Survey Results, 335 companies produce and treat nanoparticls. However, lack of data on nano-toxicity and a method for risk management and regulation on nanoparticles and the standard test method are not sufficient. Protective equipment selection guidelines for nanoparticles are not established. It is required to carry out respirator efficiency test against nanoparticles. This study was performed to evaluate filtration efficiency and manikin-based total inward leakage of particle filtering mask using in Korean country challenged with silver nanoparticles. Methods: We investigated filtration efficiency and total inward leakage of 7 respirator with silver nanoparticle. Results: The geometric mean diameters of Silver nanoparticles were 30 ㎚and number concentration were about 106 #/㎤. Filtration efficiency of six of the seven particle filtering masks was more than 98% and one particle filtering masks filtration efficiency was 94.9%. The filtration efficiency of particle filtering masks to 20 ㎚silver nanoparticels was highest. Artificial breathing machine with manikin based total inward leakage were 7.6% ~ 42.3%. Conclusions: The results of this study nano-silver filter efficiency was high but the total inward leakage was higher than filter penetration. Therefore, education on how to wear a respirator should be demanded. Especially for workers handling nanoparticles and toxic material, user seal checking and fit test must be performed.

        • KCI등재

          산화란타늄, 산화네오디뮴이 세포독성 및 DNA손상에 미치는 영향

          김종규 ( Jong Kyu Kim ), 김수진 ( Soo Jin Kim ), 강민구 ( Min Gu Kang ), 정용현 ( Young Hyun Chung ) 한국산업위생학회 2013 한국산업보건학회지 Vol.23 No.2

          Objectives: The present study investigated cytotoxicity and DNA damage in human lung cells in vitro. Methods: Neodymium oxide and lanthanum oxide were dispersed by ultrasonic treatments. The assay was performed with MRC-5 (Human male fetus lung cell). Cytotoxicity and comet assay of lanthanum oxide and neodymium oxide were measured after 24 and 48 hours incubation. Results: After 24 hours of exposure to rare earth metals, the cytotoxicities of lanthanum oxide in more than 1μM concentration groups were significantly increased when compared to the control group, but the cytotoxicities of neodymiun oxide in more than 100 μM concentration groups were statistically increased. After 48 hours exposure, cytotoxicities of both materials were statistically increased in 100,000 μM concentration groups. Olive tail moments of the lanthanum oxide treated group were significantly increased when compared to the control group. Conclusions: The cytotoxicity of lanthanum oxide was higher than that of neodymium oxide. The DNA of MRC-5 cells treated with lanthanum oxide for 48 hours were significantly damaged.

        • KCI등재후보

          GIS를 이용한 해도정보의 활용방안 연구

          김종규(JONG-KYU KIM), 김정현(JUNG-HYUN KIM) 한국해양공학회 2003 韓國海洋工學會誌 Vol.17 No.1

          A nautical chart is an essential material not only for the safety of navigation at sea, but also for the research and understanding of the ocean. Paper nautical charts have been used for navigational purposes for several hundreds years, but it was shown that paper nautical charts have certain limitations, because the speed of ships has increased dramatically, and Global Positioning Systems (GPS) are now available at sea. Thus, the development of digital charts has been conducted by a number of countries. As a result, the International Hydrographic Organization (IHO) has developed a standard format, Special Publication No. 57 (S-57), as a guideline for the production of electronic navigational charts (ENC). Even though ENCs are a state-of-the-art navigational tool, because they are produced on the basis of Geographical Information System (GIS) technology, they can be utilized for various purposes. In this paper, the usage of paper nautical charts, ENCs, and digital charts is reviewed. Also, the various application systems that make use of electronic navigational charts and digital charts, as a basic geographical source of information, will be examined on the basis of GIS.

        • KCI등재

          크리깅 근사모델을 이용한 마이크로 자이로스코프의 구조설계

          김종규(Jong-Kyu Kim), 이권희(Kwon-Hee Lee) 한국기계가공학회 2008 한국기계가공학회지 Vol.7 No.4

          The concept of robust design was introduced by Dr. G. Taguchi in the late 1940s, and his technique has become commonly known as the Taguchi method or the robust design. In this research, a robust design procedure for microgyroscope is suggested based on the kriging and optimization approaches. The kriging interpolation method is introduced to obtain the surrogate approximation model of true function. Robustness is calculated by the kriging model to reduce real function calculations. For this, objective function is represented by the probability of success, thus facilitating robust optimization. The statistics such as mean and variance are obtained based on the reliable kriging model and the second-order statistical approximation method.

        • KCI등재후보

          청소년 엘리트 선수들의 도핑의식화 운동영양 보조물 섭취의 관련성

          김종규 ( Jong Kyu Kim ), 강성기 ( Seoung Ki Kang ), 천유석 ( Yoo Seouk Chun ), 정승호 ( Seung Ho Jung ) 한국운동영양학회 2010 Journal of exercise nutrition & biochemistry Vol.14 No.4

          Dietary supplement uses and information about anti-doping knowledge in elite adult athletes have been reported in various countries including Canada. United Kingdom, Singapore and Republic of Korea, However, no study has been reported to investigate dietary supplement use and information about anti-doping knowledge in Korean elite junior athletes. Therefore, the purpose of this study was to investigate Korean elite junior athletes` anti-doping knowledge and awareness with their dietary supplement uses and patterns. 572 males and 156 female junior elite athletes from physical education high schools in 4 different cities in Korea provided written consents before their participation in the study. Among them, 44% reported that they take dietary supplements for reasons of maintaining physical strength (45%), recovering quickly from training (29%) and improving muscular strength (24%). Male and female athletes reported to receive dietary supplement information mostly from their coach (43%) and parents (58%) respectively. Our data showed that the most taken dietary supplement is herbal products (men: 36% and women: 34%), including oriental medicine, followed by vitamin (men: 26% and women: 32%), amino acids (men: 8% and women: 11%), minerals (men & women: 7%) and creatine (men: 9% and women: 4%). Our data showed that 84% reports their anti-doping awareness as less than 3 (below than normal), while 16% reports higher level of anti-doping awareness. In conclusion, this study provided information about Korean elite junior athletes from 4 different cities in Korea, resulting in that special education necessitates them being aware of anti-doping and dietary supplement use.

        • KCI등재

          조류발생 제거기술 동향 및 친환경적 조류 제어기술 방안

          김종규 ( Jong Kyu Kim ), 강동근 ( Dong Geun Kang ), 여우석 ( Wooseok Yeo ), 김헌희 ( Hun Hee Kim ) 한국수처리학회 2017 한국수처리학회지 Vol.25 No.1

          Harmful algae blooming(HAB) is transient increases or accumulations of algae in river, reservoir and marine environments that cause some degree of negative effect to aqutatic systems or human health. Some algae such as cyanobacteria naturally produce toxins. Cyanobacteria in freshwater systems form high biomass blooms and may produce toxins, and have impacted human health, destroyed aquatic organisms and harmed fishes and other industries in Korea and worldwide. To control HAB effectively, physical, chemical, and biological techniques can be applied directly. However, application of this single treatment technique is very likely to cause secondary pollution problems in the aquatic ecosystem. Therefor, as suggested in this study, it is considered that HAB will be controlled in an eco-friendly and highly efficient manner by using a hybrid- technology that applies appropriate processing techniques to each stage of algae initial occurring and algae generation. In other word, it will be possible to restore ecological function and contribute to sustainable water quality management by spreading environmentally friendly technologies through the commercialization of environmentally friendly technologies. In order to do this, research on biological methods and chemical salvation methods, in which economics and application cases are continuously ensured, must be pursued. It is also necessary to construct an early warning system for algae that allows for more systematic monitoring so that an effective control method for the occurrence of algae can be applied.

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