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김재환,김영록,김해영,Kim, Jae-Hwan,Kim, Young-Rok,Kim, Hae-Yeong 한국식물생명공학회 2011 식물생명공학회지 Vol.38 No.2
1994년 처음으로 GM 토마토인 Flavr Savr가 시장에 나온 이후, 2010년 현재 140여 품목의 GM식물이 전 세계적으로 상업화되었다. GM식물들에 대한 안전성 승인여부의 확인 및 표시제관리를 위하여 이들 GM식물내로 도입된 삽입유전자의 정보를 이용한 검정방법이 도입되었으며, 또한 도입유전자의 발현된 단백질을 분석하기 위하여 정성 및 정량을 위한 면역학적 방법이 도입되었다. 본 총설에서는 국내 외적으로 개발된 콩, 옥수수, 카놀라, 면화 등의 GM식물에 적용된 multiplex PCR, real-time PCR 방법과 최신 개발 중인 microarray, 나노기술 등을 활용한 방법들을 조사하였다. Since the first commercial GM plant, the FlavrSavr tomato, authorized in 1994, more than 140 GM plants were authorized for marketing globally. For the authorization and labelling of GM plants, the detection methods for genes introduced and proteins expressed in GM plants were developed qualitatively and quantitatively. This review presented the detection methods, conventional PCR, multiplex PCR and real-time PCR, for soybean, maize, canola and cotton as the dominant GM plants. Also, microarray assay and nanotechnology as new approaches for detection methods for GM plants were investigated.
김재환,한민수,이장존,송치영,이재만,김민지,이명성,Kim, Jae-Hwan,Han, Min-Su,Lee, Jang-Jon,Song, Chi-Young,Lee, Jae-Man,Kim, Min-Ji,Lee, Myeong-Sung 국립문화재연구소 2009 保存科學硏究 Vol.30 No.-
Stone cultural heritages are mostly situated in field, so they are damaged from mechanical, chemical and biological weathering, and their degree of strength is also weaken. Therefore, the scientific conservation of the stone cultural heritages are necessary in order to the long-term maintenance and safety conservation. In this study, we attempt to estimate on the field application of consolidants which are Wacker OH 100, Remmers KSE 300 and 1T1G according to the quality of the rocks. Based on the ultrasonic velocity, conditions of weathering damage before treatment are mainly ranked completely weathering condition (CW) or partly highly weathering condition (HW). After the first treatment, weathering damage conditions are changed by the high weathering condition (HW). These results shows that the internal pores of stone are filled with consolidant materials, so average ultrasonic velocities increases after treatment. And Remmers KSE 300 which is consolidant material is highest rate of increase of average ultrasonic velocities.
김재환,이명성,이재만,이미혜,박성미,Kim, Jae-Hwan,Lee, Myeong-Seong,Lee, Jae-Man,Lee, Mi-Hye,Park, Sung-Mi 국립문화재연구소 2010 保存科學硏究 Vol.31 No.-
This study were performed thermal shock test for four kind of different rocks (Iksan granite, Namsan granite, Jeongseon marble, Yeongyang sandstone), and according to heating temperature($400^{\circ}C$, $600^{\circ}C$) on samples were investigated physical properties such as specify gravity, porosity, p-wave velocity. As a result, the tendency was appeared that porosity increased, and specific gravity and p-wave velocity decreased at a more higher temperature. But, the situation of change appeared characteristic according to temperature and rock types. In the case of Yeongyang sandstone, it appeared in especially porosity increasing at $400^{\circ}C$. The specific gravity was little change in the all the rock at $400^{\circ}C$ but the decreased at $600^{\circ}C$. Therefore the specific gravity in the temperature range is due to the relatively small impact on the change is expected. Porosity of the granite at $400^{\circ}C$ changes little. but marble in the rate of change is large. Conversely, the sandstone porosity decreased. At $600^{\circ}C$ increased porosity in all of rocks. particularly sandstone the smallest increase in porosity. Experiments showed that p-wave velocity measured through dry rocks was sensitive to quantify the thermal damage. The p-wave velocity of all rocks decreased with increasing temperature. In the relation between porosity and p-wave velocity, p-wave velocity decreased with increasing porosity. On the other hand, in case of Yeongyang sandstone p-wave velocity decreased with decreasing porosity. thus, development of microcracks more affects p-wave velocity than porosity. In this study, damage intensity was well explained with porosity and p-wave velocity values depending on temperature increase.
김재환,김상우,조원모,강희설,조영무,Kim, Jae-Hwan,Kim, Sang-Woo,Cho, Won-Mo,Kang, Hee-Seol,Cho, Young-Moo 한국축산환경학회 1996 축산시설환경학회지 Vol.2 No.2
The objective of this study was to estimate the investment limit in equipment capital by Capital Recovery Method. The data used was collected from 23 Hanwoo(Korean Native Cattle) farms in 1994. The necessary time for herd size expansion was average 5 years from opening(9.7 heads) to 50 heads, 4 years from 50 to 100heads, 3 years from 100 to 150 heads, and 2 years from 150 to 200 heads, respectively. It took 14 years from opening to 200 heads of herd size. The debts for fattening cattle farms were 15.2million won for the size of 50 heads, 37.1 million won for the size of 100 heads and 89.0 million won for the size 200 heads, respectively. Average rates of debt interest were 5.1 % 7.2 and 10.8, correspendingly. As the hear size increased, debt interest rates also increased due to government funds limit. The investment limits in equipment capital perfarm household were 77.7 million won for 50 heads, 135.1 for 100 heads, and 294.3 for 200 heads for 5,500 won per kg liveweight, 70.7 million won for 50 heads, 122.6 for 100 heads and 269.3 for 200 heads for 5,000 won per kg liveweight, 63.6 million won for 50 heads 110.0 for 100 heads and 244.2 for 200 heads for 4,500 won per kg liveweight.