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과산화수소에 의한 산화스트레스가 섬유주세포의 노화에 미치는 영향
김재우,김신후,이재형,Jae Woo Kim,Sin Hoo Kim,Jae Hyung Lee 대한안과학회 2008 대한안과학회지 Vol.49 No.10
Purpose: To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence. Methods: Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-β-gal assays, respectively. Results: Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence. Conclusions: Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma. J Korean Ophthalmol Soc 2008;49(10):1665-1670
은행잎추출물이 배양된 테논낭 섬유아세포의 증식에 미치는 영향
김재우,김성재,강현구,Jae Woo Kim,M,D,Seong Jae Kim,M,D,Hyun Ku Kang,M,D 대한안과학회 2006 대한안과학회지 Vol.47 No.4
Purpose: To investigate the effect of Ginkgo biloba extract (GBE) on the proliferation of cultured human Tenon capsule fibroblasts (HTCF). Methods: Free radical scavenging activity of GBE was assessed with a DPPH assay. Primarily cultured HTCF were exposed to 10 and 100 ?g/ml of GBE, and the effect of this extract on HTCF survival was assessed. Following 48 hr exposure to the media with or without serum, cellular survival and nitrite production were assessed by MTT and Griess assays. To evaluate whether GBE had a cytoproptective effect, HTCF were cultured in a combination of GBE and either sodium cyanide or hydrogen peroxide. Results: GBE showed free radical scavenging activity. GBE increased the cellular survival of HTCF significantly in a dose-dependent manner and provided a cytoprotective effect when cells were exposed to sodium cyanide or were deprived of serum, but not when hydrogen peroxide was added to the medium. GBE decreased nitric oxide production but not to a statistically degree. Conclusions: GBE promotes proliferation of HTCF and has a cytoprotective effect in serum-deprived or hypoxic conditions. This suggests that GBE may be involved in the regulation of conjunctival wound healing by increasing the survival of HTCF.
김재우,김예상,김영석,김상범,Kim, Jae-Woo,Kim, Yea-Sang,Kim, Young-Suk,Kim, Sang-Bum 한국건설관리학회 2008 건설관리 : 한국건설관리학회 학회지 Vol.9 No.5
생산성은 모든 생산조직의 견실성 및 생산 활동의 효율성 평가를 위한 매우 중요한 경영 지표 중 하나로써 생산성의 기능은 건설 산업에 있어서도 예외일 수 없다. 그러나 국내의 경우 생산성의 중요성과 그 기능에도 불구하고 건설 생산성 관리와 관련된 사항은 대부분 현장관리자의 경험과 직관에 의존하고 있으며 생산성 데이터를 데이터베이스화하여 공사계획 및 관리에 적극활용하지 못하고 있다. 이러한 문제는 생산성 데이터와 관련된 정보의 수집 및 분석 방법이 체계적이지 못한 결과로, 생산성 데이터의 효과적인 활용을 위해 우선적으로 WBS개선이 이루어져야 한다. 따라서 본 연구에서는 연구문헌 분석, 해외사례 분석으로 연구에 적용함에 따른 적합여부 및 개선사항을 도출하고, 현장 인터뷰를 통해 현장여건을 반영한 후, 설정된 기본방향을 바탕으로 생산성 데이터 수집 및 분석을 위한 WBS를 구축하였다. 마지막으로 생산성 데이터 수집 및 분석을 위한 체계로의 검증을 실시하였다. 향후 구축된 WBS를 통해 수집된 생산성 데이터는 OLAP을 이용한 생산성 분석 및 Data Mining을 이용한 생산성 예측에도 활용될 것으로 기대된다. Productivity is one of key management indexes for evaluating soundness of a manufacturing organization and its efficiency. In many aspects of productivity management in the construction industry, however, intuition of an experienced field manager still plays a greater role while productivity data is not utilized efficiently for the construction management purposes, because the collection and analysis of the productivity-related information are not systematic. Lack of systematic method in collecting and analyzing the productivity data results in such problems. The existing WBS should therefore be improved to solve them. The authors developed a new WBS for productivity data collection and analysis by following the research direction that was determined by literature reviews, overseas cases, and interviews with field engineers. The new breakdown structure was then evaluated for its feasibility as a productivity analysis framework. It is expected that the productivity data collected by the WBS will be used for OLAP and mining for future productivity forecast.
프로스타글란딘F2α가 배양된 섬모체근세포의 산화질소 생성에 미치는 영향
김재우,김윤영,김근해,Jae Woo Kim,M,D,Yoon Young Kim,M,D,Keun Hae Kim,M,D 대한안과학회 2005 대한안과학회지 Vol.46 No.1
Purpose: To investigate whether prostaglandin F2α (PGF2α) affects on the production of nitric oxide (NO) in cultured ciliary muscle (CM) cells. Methods: Following primary culture of CM cells from porcine eyes, the cells were exposed to PGF2α (PhXA85, latanoprost free acid) with and without pretreatments of NO synthase inhibitor (L-NAME, Nω- Nitro-L-arginine methyl ester) and cyclooxygenase inhibitors (indomethacin and dexamethasone) for 3 days. The cellular survivals were also evaluated in serum-deprived and hypoxic conditions induced by sodium cyanide. The cellular survival and nitrite production were assessed by MTT and Griess assay, respectively. Results: PGF2α enhanced the production of NO significantly in cultured CM cells in a dose-dependent manner, while various inhibitors abolished this effect. PGF2α was not cytoprotective in serum-deprived and hypoxic conditions. Conclusions: The current results suggest that PGF2α potentiates NO production but is not cytoprotective in CM cells. This PGF2α-induced NO production in CM cells may be involved in the regulation of uveoscleral outflow.
통합 모델 설계 방식 기반 통신 프로토콜 개발을 위한 SDL-OPNET 모델 변환 기법
김재우,김태형,Kim, Jae-Woo,Kim, Tae-Hyong 대한임베디드공학회 2010 대한임베디드공학회논문지 Vol.5 No.2
Although both functional verification and performance evaluation are necessary for the development of effective and reliable communication systems, they have been often performed independently; by functional modeling with formal language tools and by performance modeling with professional network performance evaluation tools, respectively. Separate and repeated modeling of one system, however, would often result in cost increase and inconsistency between the models. This paper proposes an integrated model design approach in order to overcome this problem that evaluates the performance of a communication protocol designed in SDL with SDL-OPNET model conversion. The proposed technique generates OPNET skeleton code from Tau-generated C code of the SDL model by analyzing the relations between SDL and OPNET models. IEEE 802.2 LLC protocol was used as an example of model conversion to show the applicability and effectiveness of the proposed technique.
Target DNA 염기서열 내에 존재하는 비상동성 간격이 상동성재조합을 이용한 클로닝 빈도에 미치는 영향
김재우,도은주,윤세련,정윤희,윤영호,임선희,선우양일,박인호,Kim Jae-Woo,Do Eun-Ju,Yoon Se-Lyun,Jeong Yun-Hee,Yoon Young-Ho,Leem Sun-Hee,Sunwoo Yangil,Park In-Ho 한국미생물학회 2005 미생물학회지 Vol.41 No.4
Transformation-associated recombination (TAR) 클로닝 법은 복잡한 게놈으로부터 염색체 내의 특정부위나 유전자를 선택적으로 분리할 수 있다. 이 방법은 목적 유전자에 근접한 작은 게놈DNA 염기서열 정보를 필요로 한다. 이 기술은 효모의 spheroplast transformation을 시키는 동안 목적으로 하는 유전자의 5' 또는 3' 서열을 포함하고 있는 TAR vector와 게놈DNA사이에서 일어나는 상동성재조합에 의해 이루어진다. 본 연구에서는 plasmid 모델시스템을 이용하여 target hooks 내에 존재하는 비상동성 염기서 열이 상동성재조합에 미치는 영향을 조사하였다. plasmid에 존재하는HIS3유전자와 변형시킨 his3-TRP1-his3 단편 사이의 상동성재조합의 효율은 $Ura^+$ 형질전환체의 형질분석에 의해 이루어졌다. $Ura^+$ 형질전환체의 수는 7종류의 서로 달리 변형된 his3-TRP1-his3 단편들을 사용하였을 매 거의 동일하게 나타났다. 그러나 $Trp^+His^+$ positive recombinants의 빈도는 변형된 his3-TRP1-his3 단편 내에 비상동성 영역에 부정확한 간격을 지닐 때 현저한 감소를 나타내었다. 이러한 결과로서, 부정확한 간격이 target hook과 substrate DNA 사이에 일어나는 상동성재조합을 방해하는 것으로 사료된다. 그러므로 이종간의 상동유전자를 클로닝 할 때에는 target hook내의 비상동성 염기서열이 존재한다면 이것이 정확한 간격을 지니는지 여부를 중요란 요인으로 고려해야 한다. Transformation-Associated Recombination (TAR) cloning technique allows selective isolation of chromosomal regions and genes from complex genomes. The procedure requires knowledge of relatively small genomic sequences that reside adjacent to the chromosomal region of interest. This technique involves homologous recombination during yeast spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences. In this study, we examined the effect of non-homologous spacing sequence in target hooks on homologous recombination using a plasmid model system. The efficiency of homologous recombination between the modified his3-TRP1-his3 fragments and HlS3 gene on plasmid were analyzed by the characterization of $Ura^+$ transformants. The numbers of $Ura^+$ transformant showed same level when seven different modified his3-TRP1-his3 fragments were used. But the percentage of positive recombinants. $Trp^+His^-$, dramatically decreased when used the modified his3-TRP1-his3 fragments contained incorrect spacing of nonhomologous region. As a result, we suggest that incorrect spacing inhibits the homologous recombination between target hook and substrate DNA. Therefore, we should consider the correct spacing in target hook when the target hook are used for cloning of orthologue gene.
김재우,Kim, Jae-Woo 한국정보기술전략혁신학회 2005 情報學硏究 Vol.8 No.2
The S.G. Policy form contains the words "the good ship or vessel called the.....". The words "good ship" mean that the ship is deemed to be seaworthy at the commencement of the voyage and this was very necessary in the day when a separate policy was issued for each voyage. In fact the warranty do seaworthiness still applies to all voyage policies. Nevertheless, the law does not apply an absolute warranty of seaworthiness to a time policy, so a ship is not required to be seaworthy at the time the hull policy is effected. The implied warranty of seaworthiness does not extend to good, for the underwriter is not responsible for their condition, apart fro the action of the perils insured against. The implied warranty of seaworthiness is limited to the vessel herself, and does not extend to a lighter or other craft used to convey the goods to the ship. The underwriters waive any breach of the implied warranties of the seaworthiness of the ship and fitness of the ship to carry the subject-matter insured to destination, unless the assured or their servants are privy to such unseaworthiness of unfitness.
쓰레기 소각재 사용 콘크리트의 품질특성에 대한 실험적 연구
김재우,최재진,문대중,Kim, Jae-Woo,Choi, Jae-Jin,Moon, Dae-Joong 한국건설순환자원학회 2014 한국건설순환자원학회 논문집 Vol.2 No.4
쓰레기 소각재(소각재)를 콘크리트용 재료로 재활용하기 위하여 소각재의 품질특성에 대하여 분석하였으며, 소각재를 사용한 콘크리트의 유동특성 및 강도특성에 대하여 검토하였다. 소각재의 화학성분은 CaO 성분이 50%이상을 차지하고 있었으며, 소각재의 누적입도분포 50%의 평균입경은 $25{\mu}m$ 정도를 나타내었다. 소각재 IA1의 입자형상은 미세입자가 뭉쳐져 구형상을 나타내고 있으며, 소각재 IA2의 입자형상은 미세입자가 판상 형상으로 겹쳐져 있는 구조로 제조공정에 따라 다르게 나타났다. 소각재를 사용하므로써 콘크리트의 품질특성에도 크게 영향을 미쳤으며, 소각재 IA2를 사용한 경우 슬럼프가 증가하고 고성능감 수제 사용량이 감소하였으나, 압축강도 발현은 저하하였다. 소각재 IA2와 규조분말을 병용하여 혼합하므로써 목표슬럼프를 얻기 위한 고성능감수제의 사용량은 증가하였으나, 재령 28일 압축강도는 향상되는 효과가 있었다. 또한, 압축강도와 인장강도와의 비는 CEB-FIP 코드의 85%~105% 범위내에 있었다. The Quality of municipal solid waste incineration ash (incineration ash) was analyzed for the purpose of the reusing for concrete material. The folwability and strength properties of concrete mixed with incinerator ash were investigated. CaO component was included more than 50% in chemical component of incinerator ash, mean size of 50% accumulated particle distribution of incinerator ash was about $25{\mu}m$. Particle shape of incinerator ash ($IA_1$) was massed the round shape with fine particle, particle shape of incinerator ash ($IA_2$) was piled up the sheet shape according to manufacture procedure. The Quality of concrete was effected by use of incinerator ash. When the incinerator Ash ($IA_2$) was used, slum of concrete was increased and dosage of high range water reducing agent was reduced. However, strength development of concrete was decreased. Dosage of high range water reducing agent was increased by combined use of incinerator ash ($IA_2$) and diatomite powder, but strength development of concrete was improved. Ratio of compressive strength and tensile strength was in the range 85%~105% of CEB-FIP model code.
일산화질소가 섬유주세포에 의한 교원질 겔의 수축에 미치는 영향
김재우,장우석,이수윤,Jae Woo Kim,M,D,Woo Seok Chang,M,D,Su Yoon Lee,M,D 대한안과학회 2007 대한안과학회지 Vol.48 No.11
Purpose: To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs). Methods: After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively. Results: Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05). Conclusions: NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow.
김재우,현진오,현민경,신원용,임성우,최은영,Kim, Jae-Woo,Hyun, Jin-Oh,Hyun, Min-Kyung,Sin, Won-Yong,Lim, Seong-Woo,Choi, En-Young 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.3
The purpose of this study is to develop an aproach to diagnosis and treatment of sequelae after removal of vestibular schwannoma through Oriental Medicine. An eighty year-old woman with sequelae after removal of vestibular schwannoma was observed and treated. She had difficulty of hearing, facial nerve palsy, dizziness and was generally weak. She was treated with herb medications for these symptoms. Having been treated for 27 days in hospital her symptoms improved. This report provides evidence for application of herbal medicine in treatment of sequelae. However, more cases are required for an in depth study of oriental treatments for this ailment in order to open this method of treatment to general application.