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저위전방절제술 후에 발생한 특발성 후복막강 섬유증과 모자 용종증
송림화 ( Lim Hwa Song ),전병우 ( Byung Woo Jhun ),박지현 ( Ji Hyeon Park ),김다민 ( Da Min Kim ),장동경 ( Dong Kyung Chang ),김영호 ( Young Ho Kim ),김재준 ( Jae Jun Kim ),김진용 ( Jin Yong Kim ) 대한소화기학회 2011 대한소화기학회지 Vol.58 No.5
An optimal treatment for cap polyposis has not been established. Several treatment approaches, including anti-inflammatory agents, antibiotics, immunomodulators, and endoscopic therapy have been described. Surgical resection of the affected colon and rectum may be indicated for patients with persistent disease. Repeat surgery is indicated in cases of recurrence after surgery. However, symptomatic polyposis may still recur, and spontaneous resolution of cap polyposis is possible. We report a case of recurrent cap polyposis complicated with retroperitoneal fibrosis after inadequate low anterior resection with a positive resection margin. Surgical approaches for the treatment of cap polyposis should be carefully considered before treatment. (Korean J Gastroenterol 2011;58:275-279)
형광영상을 이용한 옥수수 반수체 종자 선발 효율성 향상
김영욱(Younguk Kim),한정헌(Jeong Heon Han),김재영(Jaeyoung Kim),김영태(Yeongtae Kim),김년희(Nyunhee Kim),이채원(Chaewon Lee),이서연(Seoyeoun Lee),김송림(Song Lim Kim),김문종(Moon Jong Kim),류시환(Si Hwan Ryu),이홍로(Hongro Lee),지현소(H 한국육종학회 2022 한국육종학회지 Vol.54 No.4
Many studies concerning breeding maize varieties are in progress in Korea and other countries. Double haploid technology is widely used for the development of commercial maize varieties worldwide, and has also been utilized in Korea since its introduction by the Maize Research Institute, Gangwondo. We performed a study to improve the efficiency of selecting haploid maize seeds using fluorescence imaging. It was verified that anthocyanin produced by the expression of R1-nj gene can be detected by fluorescence imaging, and we developed a high-throughput method for discriminating between haploid and diploid seeds. Compared with discriminating with naked eye, this method reduced the time for discriminating haploid and diploid maize by 91.7% and increased selection accuracy by 16.8% for haploid and 2.2% for diploid maize. This method enabled the acquisition of more haploid seeds and improved the efficiency of breeding research by shortening the time involved.
애기장대(Arabidopsis thaliana)잎 절편에서 NAA와 BA에 의한 신초, 부정근, 모용 및 캘러스 형성 결정 시기
김송림,한태진,Kim, Song-Lim,Han, Tae-Jin 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2
The effect of NAA and Benzyladenine(BA) for determination times on the formation of adventitious shoots, roots trichmoes and calli in MS basal medium was investigated in leaf segments from ecotype 'Nosses' of Arabidopsis thalliana. Adventitious shoots, roots, trichomes and calli were formed fromed from leaf segments in a wede range of NAA and BA. The optimal combination of hormones for adventitious shoots formation, 20mg/L NAA for trichome formation, 100mg/L for callus formation. Inductive times for formation of adventitious shoots, roots, trichomes and calli were determined at 14, 4, 6 and 18 days respectively by periodical transfer of leaf segments from hormines containing media to hormone free medium.
벼의 CRISPR/Cas9 기반 유전자 교정을 통한 내병성 및 내재해성 증진 연구 동향
김유림(Yurim Kim),최시은(Sieun Choi),윤인선(In Sun Yoon),김송림(Song Lim Kim),모영준(Youngjun Mo) 한국육종학회 2022 한국육종학회지 Vol.54 No.4
As recent advances in gene editing technologies have enabled rapid and accurate modification of target genes, new varieties are being developed through the application of gene editing technologies in various crop species. In particular, the CRISPR/Cas9 system has become a tool of choice for gene editing because it is much more economical and efficient than previous tools such as ZFN and TALEN, and is being actively used to improve various breeding traits, including biotic and abiotic stress tolerance to overcome the limitations of conventional plant breeding technologies. In this review, we retrieved 210 papers describing the utilization of CRISPR/Cas9 in rice published between 2013 and 2021 and classified them according to the field of study and traits of interest. Further case studies were conducted on 21 and 12 research papers that reported the enhancement of biotic and abiotic stress tolerance, respectively. This demonstrated that CRISPR/Cas9-based gene editing can be highly effective in improving resistance to bacterial (bacterial leaf blight and bacterial leaf streak), fungal (blast, sheath blight), and viral (rice tungro spherical virus, rice black streak virus) diseases as well as various abiotic stresses, including drought, salinity, cold, and heat, in many cases, without diminishing important agronomic traits. As recent technological advances have begun to overcome the major limitations of CRISPR/Cas9 gene editing, such as low HDR efficiency and off-target effects, it is expected that more research on gene function and cultivar development will adopt CRISPR/Cas9 as a major gene editing tool in the future. To effectively apply such innovative technologies in crop improvement, much effort is required to establish more reasonable and detailed policies for regulating crops developed through new breeding technologies.
Delta 15 desaturase 유전자 억제에 의해 알파리놀렌산 함량이 낮은 들깨 육성
김경환(Kyung-Hwan Kim),이경렬(Kyeong-Ryeol Lee),김정봉(Jung-Bong Kim),이명희(Myoung Hee Lee),이은경(Eungyeong Lee),김년희(Nyunhee Kim),이홍석(Hongseok Lee),김송림(Song Lim Kim),백정호(JeongHo Baek),최인찬(Inchan Choi),지현소(Hyeonso 한국육종학회 2018 한국육종학회지 Vol.50 No.4
Perilla is an oilseed crop cultivated in Korea since ancient times. Due to the high α-linolenic acid content in perilla, perilla seed oil can easily become rancid. α-Linolenic acid is synthesized by two enzymes, endoplasmic reticulum-localized Δ15 desaturase (FAD3) and chloroplast-localized Δ15 desaturase (FAD7) in vivo. In order to lower the α-linolenic acid content of the seed oil without disturbing plant growth, we tried to suppress the expression of only the FAD3 gene using RNA interference, whilst maintaining the expression of the FAD7 gene. Seventeen transgenic plants with herbicide (Basta TM ) resistance were obtained by Agrobacterium-mediated transformation using hypocotyls of perilla plants. The transgenic plants were firstly confirmed by treatment with 0.3% (v/v) Basta TM herbicide, and the expression of FAD3 was measured by Northern blot analysis. The α-linolenic acid content was 10-20%, 30-40%, and 60% in two, seven, and three of the twelve T1 transgenic perilla plants which had enough seeds to be analyzed for fatty acid composition, respectively. Analysis of the fatty acid composition of T2 progeny seeds from T1 plants with the lowest α-linolenic acid content showed that the homozygous lines had 6-10% α-linolenic acid content and the heterozygous lines had 20-26% α-linolenic acid content. It is expected that the reduction in α-linolenic acid content in perilla seed oil will prevent rancidity and can be utilized for the production of high-value functional ingredients such as high γ-linolenic acid.
고압나트륨등 조사가 파프리카의 생장 및 수확량에 미치는 영향
장경만 ( Kyung-man Jang ),김세광 ( Segwang Kim ),신민경 ( Min Kyung Shin ),박규현 ( Gyu Hyeon Park ),신영자 ( Young Ja Shin ),박창록 ( Chark Lok Park ),오송림 ( Song Lim Oh ),이정현 ( Jeong Hyun Lee ) 한국농업기계학회 2019 한국농업기계학회 학술발표논문집 Vol.24 No.1
연구의 목적은 낮은 외부 광조건과 겨울철 일조시간을 연장시켜 파프리카의 생육 및 수확량에 미치는 영향을 구명하고자 수행하였다. 광원은 고압나트륨등(High pressure sodium lamps, 400W)을 이용하여 광량자속밀도 umol/ ㎡/s로 조사하였다. 전남대학교 벤로형 유리온실 1개 구역(4.8*5m)에서 파프리카 공시품종‘Scirocco’(Enza Zaden, The Netherlands)를 2018년 9월 12일 240구 암면 두 트레이에 표준양액(EC 2.5dSㆍm<sup>-1</sup>)으로 포습하여 파종하였고 본 엽이 2~3매 전개되었을 때 유묘를 표준양액(EC 3.0dSㆍm<sup>-1</sup>)으로 포습 된 코코피트배지 (100×20×15cm, Daeyoung GS, Korea)에 2018년 10월 1일에 정식하였다. 줄기밀도는 7.5stems/ ㎡로 재배하였다. HPS 처리는 정식 후 17일부터 시작하였으며 1:30~17:30까지 하루 16시간동안 외부광도가 200W/ ㎡ 이하일 때 조사하였고, 파프리카의 동적생육정보와 수확량을 조사하기 위해 주기적으로 조사 하여 대조구(무처리구)와 비교 분석 하였다. 엽 건물중은 대조구와 유의적 차이를 보이지 않았으나 줄기, 생식기관, 총건물중에서 대조구에 비해 HPS 처리구에서 각각 34%, 89%, 47%가 증가하는 유의성을 나타냈다. 초기 수확소요일은 HPS 처리구에서 20일 정도 빨랐고 단위 면적당 누적수확량은 정식 후 148일에 유의적 차이를 보였으며 HPS 처리구에서 2.992±0.528 kg/ ㎡ 대조구에서 0.785±0.168 kg/ ㎡, 177일에는 유의적 차이는 없었으나 HPS 처리구에서 4.663±1.144 kg/ ㎡ 대조구에서 3.469±0.962kg/ ㎡로 HPS처리구가 대조구에 비해 높은 수치를 보였다. HPS 처리구는 초기 수확 소요일을 앞당겼으며 초기 생육과 건물생산의 증가에 영향을 미쳤다.
고추 탄저병 정밀 탐색을 위한 영상분석 기술에 대한 연구
백정호(JeongHo Baek),김년희(Nyunhee Kim),이은경(Eungyeong Lee),이홍석(Hongseok Lee),김송림(Song Lim Kim),박상렬(Sang Ryeol Park),지현소(Hyeonso Ji),최인찬(Inchan Choi),김경환(Kyung-Hwan Kim) 한국산업정보학회 2020 한국산업정보학회논문지 Vol.25 No.5
전세계적으로 널리 재배되는 고추 (Capsicum annuum)는 한국에서 소비가 많은 채소류 중 매우 중요한 작물중 하나이다. 고추는 생육기간 동안에 고추 탄저병에 심한 피해를 입어 방제나 저항성 품종에 대한 연구가 중요하다. 본 연구에서는 탄저병에 저항성이 있는 고추 4품종과 민감성을 가진 1품종에 대해 K1탄저균을 접종하였으며, 시간이 지나면서 나타나는 병 면적을 촬영하여 프로그램을 통해 정량화한 내용을 분석하였다. 고추과일 면적과 병면적의 비율을 통해 감수성 품종인 An-S는 약 40%로 약하게 나타났으며, 저항성 품종인 An-12R (23%), AR-Tan (21%), An-9R (19%)로 비교적 강하게 나타났고, PBC81는 약 11%로 탄저균에 강한 병 반응을 보였다. 이와 같은 정량적인 수치는 탄저병 품종이나 탄저균에 대한 저항성 연구를 수행하는데 비교할 수 있는 기초자료로 활용할 수 있다. One of the most important vegetables consumed in Korea, chili peppers (Capsicum annuum) are widely cultivated around the world. Chili peppers have been severely damaged by anthracnose during their growth, so it is important to study prevention and resistance varieties. K1 anthracnose was inoculated against four cultivar of chili peppers that are resistant to anthracnose and one cultivar that is sensitive. The area of the disease that appeared over time was photographed and quantified through the program was analyzed. Through the ratio of the area of chili pepper fruit and the area of the bottle, the sensitive variety An-S showed weak reactions to anthracnose with about 40%, the resistant variety An-12R (23%), An-Tan (21%), and An-9R (19%), and PBC81 showed a strong response to anthracnose with about 11%. These quantitative value can be used as a basis for comparison in conducting resistance studies for new varieties.
한태진(Han Tae Jin),김인현(Kim In Hyeon),김송림(Kim Song Lim),김준철(Kim Joon Chul),임창진(Lim Chang Jin),진창덕(Jin Chang Duck) 강원대학교 기초과학연구소 1999 기초과학연구 Vol.10 No.-
The effect of auxins and cytokinins on the formation of adventitious shoots, adventitious roots, trichomes, and calli in MS basal medium was investigated in leaf segments from ecotype Columbia of Arabidopsis thallana. Adventitious shoots, adventitious roots, trichome, and calli were formed from leaf segments by a wide range of hormone concentrations and combinations. Adventitious shoots were formed respectively in treatment with 0.1 mg/L IAA and 10 mg/L BA. Adventitious roots were formed in treatments with low concentration of IAA and NAA. Trichomes and calli were formed by increasing the concentration of IAA and NAA. The optimal combination was 0.5 mg/L NAA and 0.1 mg/L BA for trichome formation, 10 mg/L NAA and 10 mg/L BA for calli formation. When NAA was treated alone in culture media, adventitious roots were formed in 0.1 mg/L, trichomes were formed in 2.0 mg/L, and calli were formed in 1.0 mg/L Inductive time for formation of adventitious roots, trichomes and calli were determined at 6,7 and 18 days respectively by periodical transfer of leaf segments from NAA containing medium to NAA free medium. Key words: Adventitious shoot, Iaa kinetin, BA 2,4-D
MutMap 분석에 의한 벼 왜성 돌연변이 계통의 변이 유전자 탐색
오준(Jun Oh),천경성(Kyeong-Seong Cheon),강도유(Do-Yu Kang),김송림(Song Lim Kim),이은경(Eungyeong Lee),김년희(Nyunhee Kim),오효자(Hyoja Oh),최인찬(Inchan Choi),백정호(Jeongho Baek),윤인선(In Sun Yoon),김경환(Kyung-Hwan Kim),정남진(Nam-J 한국육종학회 2020 한국육종학회지 Vol.52 No.1
A dwarf mutant rice line was selected from an Ac/Ds insertion mutant population and named dwf1. The phenotype of F1 and F2 plants derived from a cross between dwf1 and Dongjin indicated that a single recessive gene is responsible for the mutant phenotype, and we named this gene dwf1. Resequencing of the dwf1 line and Dongjin (wild type) revealed 42,386 homozygous single nucleotide polymorphisms (SNPs) between dwf1 line and Dongjin. MutMap analysis was performed by sequencing a DNA pool prepared from 100 mutant type plants in the dwf1/Dongjin F2 population, and it was found that the dwf1 gene was located in the 23 ~ 30 Mbp region on chromosome 4. In this region, we found a non-synonymous SNP in the Os04g0469800 gene, which was reported as D11 gene encoding a cytochrome P450 family protein involved in the biosynthesis of brassinosteroids (BRs). This SNP was regarded as the causative SNP for the dwf1 phenotype, and the dwf1 gene is a novel allele of D11. We performed mapping of the dwf1 gene with five SNP markers on chromosome 4 with 190 dwf1/Dongjin F2 plants. The phenotype of F2 plants was completely co-segregated with genotypes of the J10402 marker, which was developed based on the non-synonymous SNP in the D11 gene. These results will contribute to the study of the molecular biological functions of the D11 gene and BRs.