홍삼사포닌 투여의 다낭성난소에 의한 불임 치료효과 및 기작연구

김세은,오동민,심경미,정문진,임성철,나승열,이윤렬,강성수,문창종,김종춘,김성호,배춘식,Kim, Se-Eun,Oh, Dong-Min,Sim, Kyung-Mi,Jeong, Moon-Jin,Lim, Sung-Chul,Nah, Seung-Yeol,Lee, Yun-Lyul,Kang, Seong-Soo,Moon, Chang-Jong,Kim, Jong-Choon,Kim 한국현미경학회 2009 Applied microscopy Vol.39 No.1

사람에서의 PCO와 몇몇 측면에서 유사한, 실험적으로 유발된 다낭성난소(polycystic ovary, PCO)는 장기간 작용하는 estradiol valerate (EV)에 의해 유발된다. 랫드에서 스테로이드로 유발한 PCO 모델에서 홍삼 총사포닌의 역할에 대한 우리의 이전연구는 전침이 난소의 nerve growth factor (NGF) 농도를 조절하는 것을 검증하였다. 실제로 PCO와 관련된 난소 기능부전의 병리기전에서 신경성 요소의 관련성은 난소로의 교감신경유출(sympathetic outflow)의 증가로 인해 높아진다. 따라서 본 연구에서는 치료제로서의 GTS 투여가 PCO를 유발한 랫드에서 교감신경 활성을 조절할 것이라는 가설을 시험해보고자 하였다. 본 연구에서는 스테로이드로 유발한 PCO에 내재하고 있는 병태생리학적 과정과 연관된 NGF 단백질과 NGF mRNA를 분석함으로써 이루어졌다. PCO가 유발된 랫드에서 EV 대조군은 oil 대조군과 비교하였을 때 유의적으로 높은 NGF mRNA의 발현을 나타내었으며, GTS 투여군은 EV 대조군에 비해 유의적으로 낮은 NGF mRNA의 발현을 나타내었다. 그러나 NGF 단백질은 oil 대조군에 비해 EV 대조군과 GTS 투여군에서도 유의적인 변화를 발견할 수 없었다. 이러한 결과는 EV가 설치류에서 낭종 형성과 무배란을 유발하는 병리학적 단계의 구성요소일 수 있는 난소의 신경향성 단계(neurotrophic state)를 조절한다고 생각할 수 있다. Experimental induction of polycystic ovary (PCO) resembling some aspects of human PCO syndrome was produced using the long-acting compound estradiol valerate (EV). Our previous study on the role of Korean red ginseng total saponins (GTS) in a steroid-induced PCO rat model demonstrated that electro-acupuncture modulates nerve growth factor (NGF) concentration in the ovaries. In fact, the involvement of a neurogenic component in the pathology of PCO-related ovarian dysfunction is preceded by an increase in sympathetic outflow to the ovaries. In the present study, we tested the hypothesis that therapeutic GTS administration modulates sympathetic nerve activity in rats with PCO. This was done by analyzing NGF protein and NGF mRNA expression involved in the pathophysiological process underlying steroid-induced PCO. EV injection resulted in significantly higher ovarian NGF mRNA expression in PCO rats compared to control rats, and PCO ovaries were counteracted by GTS administration with significantly lower expression of NGF mRNA compared to EV treated ovaries. However, NGF protein was unaffected in both EV and GTS treated ovaries compared to control rats. These results indicate that EV modulates the neurotrophic state of the ovaries, which may be a component of the pathological process by which EV induces cyst formation and anovulation in rodents.

약용 식물의 암세포 다제내성 조절 활성 검색

김세은,황방연,김영호,김영중,이경순,이정준,Kim, Se-Eun,Hwang, Bang-Yeon,Kim, Young-Ho,Kim, Young-Choong,Lee, Kyong-Soon,Lee, Jung-Joon 한국생약학회 1997 생약학회지 Vol.28 No.4

Methanol extracts from 450 plants were screened for muttidrug-resistance reversing activity using drug sensitive KB-3-1 and multidrug-resistant KB-Vl cells. Among them, the extracts of Cynanchum wilfordii, Torilis japonica, Celastrus orbiculatus, Melia toosendan and Teminialia chebula strongly potentiated vinblastine cytotoxicity in KB-Vl cells. But their cytotoxicities to both sensitive KB-3-1 and resistant KB-Vl cells were in the same order of magnitude. These results indicate that the above samples would contain the active principles which do not exert their ativity solely by cytotoxicity.

복시를 주소로 내원한 에드하임-체스터병 1예

김세은,김숙영,김근해,Se Eun Kim,Sook Young Kim,Keun Hae Kim 대한안과학회 2014 대한안과학회지 Vol.55 No.2

Purpose: We present a case of Erdheim-Chester disease (ECD) with diplopia. Case summary: A 56-year-old woman came to the hospital with a 6-week history of diplopia on left lateral gaze. The right eye showed mildly limited adduction. Humphrey automated perimetry demonstrated inferior bitemporal quadrantanopia. Orbital and brain magnetic resonance imaging revealed well-defined orbital masses in both intraconal orbits with homogenous enhancement, as well as multiple masses of homogenous signal intensity in the brain. Systemic evaluation showed involvement of the long bones, and retroperitoneum, but no involvement of the heart, or lungs. Incisional biopsy of the right orbital mass was performed. Histopathological examination showed numerous lipid-laden histiocytes and few multinucleated Touton giant cells. Immunohistochemical staining showed positivity for CD68, but negativity for CD1a, and ECD was therefore diagnosed. The patient received treatment with radiation therapy and interferon-α, but died due to sepsis secondary to urinary tract infection after 2 months. Conclusions: Except exophthalmos, diplopia may be the only initial symptom of an orbital mass. Although rare, the possibility of ECD should be considered in the differential diagnosis of both retrobulbar and orbital masses with diplopia. J Korean Ophthalmol Soc 2014;55(2):283-288

대학 캠퍼스 내 무인 식품 매점 운영에 대한 고객 요구도 분석

김세은,박민서,배현주,Kim, Se-Eun,Park, Min-Seo,Bae, Hyun-Joo 한국영양학회 2022 Journal of Nutrition and Health Vol.55 No.5

대학구성원의 식생활 개선을 위해 24시간 운영하는 '무인 식품 매점' 운영에 대한 고객 요구도를 총 329명을 대상으로 온라인 설문조사를 한 결과 조사대상자 중 남자는 31.3%, 여자는 68.7%였고, 1인 가구는 전체의 54.1%였으며, 아침식사 결식률은 39.2%였고, 섭취가 부족한 식품군은 과일류 (35.6%), 채소류 (32.8%) 순으로 높았다. 점심식사는 배달음식을 이용하는 경우가 32.2%로 가장 많았고, 점심식사로 선호하는 메뉴는 한식 (74.2%), 일품요리 (34.3%), 일식 (31.9%), 패스트푸드 (28.0%), 중식 (17.0%)의 순이었다. 점심식사와 점심식사 장소에 대한 전체적인 만족도는 평균 3.91점/5점이었고, 전체적인 만족도는 직접 조리해서 먹는 경우가 유의적으로 가장 높았고, 편의점에서 식사하는 경우가 가장 낮았으며 (p < 0.001), 가족과 식사하는 경우가 친구나 혼자 식사하는 경우보다 유의적으로 가장 높았다 (p < 0.001). 무인 매점 이용 의향은 평균 3.98점/5점으로, 직접 조리하는 경우 (3.76점)가 유의적으로 가장 낮았고, 편의점에서 식사하는 경우 (4.38점)가 유의적으로 가장 높았다 (p < 0.05). 무인 식품 매점 희망 이용 시간대는 점심시간 (54.1%), 저녁시간 (46.2%), 오후 간식 시간 (41.9%) 순이었고, 구입 희망 식품은 도시락류 (62.0%), 샌드위치류 (56.2%), 컵과일류 (54.4%), 샐러드류 (51.7%), 김밥류 (50.5%) 순이었다. 판매 예정 식품의 구입 희망 평균 가격은 도시락류는 4,991원, 샐러드류가 3,403원, 컵과일류는 2,745원, 샌드위치류는 2,609원, 김밥류는 2,235원이었다. 대학교 내 무인 매점 경영자는 무인 식품 매점의 성공적인 운영을 위해서 고객의 요구를 실시간 파악하여 고객만족을 극대화할 수 있는 효과적인 마케팅 전략을 수립해 나가야 할 것이다. Purpose: This study was performed to analyze customer needs for the operation of unmanned food stores on a university campus. Methods: A total of 329 responses were collected from customers who had purchased food at the stores. Statistical analyses were conducted using the SPSS program for frequency analysis, χ2-test, t-test, one-way analysis of variance, and Duncan's multiple range test. Results: On average, the overall satisfaction score for lunch and the eating location was 3.91 out of 5 points. Overall satisfaction was significantly higher for home cooking, and lower for eating at convenience stores (p < 0.001), and the score for eating with family was significantly higher than for eating alone or with friends (p < 0.001). On average, the intention to use an unmanned store received a score of 3.98 out of 5 points, while 'home cooking (3.76)' was significantly lower than other eating locations and eating at 'convenience stores (4.38)' was significantly higher than other eating locations (p < 0.05). The desired time of use for unmanned food stores was 'lunch (54.1%)', 'dinner (46.2%)', and 'afternoon snack (41.9%)', and the desired foods were 'doshirak (62.0%)', 'sandwich (56.2%)', 'fruit cup (54.4%)', 'salad (51.7%)', and 'kimbap (50.5%)'. There were significantly more opinions that single-person households would use unmanned food stores for dinner more than family households (p < 0.05), and significantly more willingness to purchase fruit cup (p < 0.05). The average prices (Korean won) that the subjects were willing to pay for the purchase of food were 4,991 for doshirak, 3,403 for salad, 2,745 for fruit cup, 2,609 for sandwich, and 2,235 for kimbap. Conclusion: For the successful operation of the unmanned food stores on the university campus, the store manager should grasp the customer's needs in real-time and establish an effective marketing strategy.

섬유주세포에서 베바시주맙과 라니비주맙이 일산화질소합성효소의 발현에 미치는 영향

김세은,김재우,Se Eun Kim,Jae Woo Kim 대한안과학회 2014 대한안과학회지 Vol.55 No.8

Purpose: To compare the effects of bevacizumab and ranibizumab on the expression of eNOS in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to 0.5 mg/mL bevacizumab and ranibizumab using 1% serum-containing media for 30 minutes. Expression of eNOS mRNA was assessed with RT-PCR. Additionally, after exposure to 20 ng/mL of vascular endothelial growth factor (VEGF) and 0.5 mg/mL bevacizumab and ranibizumab, the production of nitric oxide was assessed with Griess assay. Results: VEGF increased the production of nitric oxide in HTMC. Bevacizumab and ranibizumab decreased the expression of eNOS mRNA and production of nitric oxide (<em>p</em> < 0.05) in HTMC. The decrease in eNOS mRNA expression and production of nitric oxide was not significant between bevacizumab and ranibizumab (<em>p</em> > 0.05). Conclusions: In HTMC, both bevacizumab and ranibizumab decreased the expression of eNOS mRNA with no significant difference observed between the two drugs. J Korean Ophthalmol Soc 2014;55(8):1208-1212

국소성 분절성 사구체 경화증에 의한 영아형 신증후군 1례

김세은,한영심,송민섭,정우영,Kim Se Eun,Han Young Sim,Song Min Seop,Chung Woo Yeong 대한소아신장학회 1999 Childhood kidney diseases Vol.3 No.1

Focal Segmental Glomerulosclerosis(FSGS) is one of the underlying pathology of congenital and infantile nephrotic syndrome. There is no ultimate curative treatment except renal transplantation. We have experienced a case of infantile nephrotic syndrome In a 10 month old boy who presented with proteinuria and hematuria. His elder brother also suffered from nephrotic syndrome and died at the age of 18 months due to sepsis. We have diagnosed this patient with clinical manifestations, laboratory data and pathologic findings which was done by open renal biopsy. The patient expired 54 days after admission because or progressive uremia and sepsis.

디지털 프린팅을 활용한 고부가 가치 티셔츠 디자인

김세은 ( Se Eun Kim ),유영선 ( Young Sun Yoo ) 服飾文化學會 2007 服飾文化硏究 Vol.15 No.3

방송통신대학 간호학 전공과정 간호사의 수업만족도와 학업적 자기효능감이 자기주도적 학습능력에 미치는 영향

김세은(Se-Eun Kim) 한국콘텐츠학회 2016 한국콘텐츠학회논문지 Vol.16 No.11

본 연구는 방송통신대학 간호학 전공과정 간호사를 대상으로 수업만족도, 학업적 자기효능감, 자기주도적 학습능력을 확인하고 자기주도적 학습능력에 미치는 영향을 파악하고자 수행하였다. 자료수집은 학업을 병행하고 있는 방송통신대학 간호학 전공 과정에 있는 간호사 231명을 대상으로 하였다. 자료분석은 퉁계분석 프로그램을 이용하여 기술통계, 상관관계분석, 다중회귀분석을 이용하였다. 연구결과 방송통신대학 간호학 전공 과정 간호사의 수업만족도 및 자기주도적 학습능력, 학업적 자기효능감 간에는 모두 유의한 양의 상관관계가 있는 것으로 나타났으며, 수업만족도와 자기주도적 학습능력은 학업적 자기효능감에 영향을 미치는 요인으로 파악되었다. 이에 본 연구 결과가 방송통신대학 간호학 전공과정 간호사의 자기주도적 학습능력 향상 프로그램 개발의 근거 자료로 활용될 수 있을 것으로 기대하며, 개발된 교육 프로그램의 타당성과 효과성을 분석하는 중재 연구 또한 필요함을 제안한다. This study was performed by targeting nurses majoring in nursing science of Korea National Open University in order to examine the level of their class satisfaction, academic self-efficacy and self-directed learning ability and to identify their effects on self-directed learning ability. Data was collected by targeting 231 nurses majoring in nursing science of Korea National Open University and for data analysis, technical statistics, correlation analysis and multiple regression analysis were performed through statistical analysis program. This study revealed that there was significant correlation among the levels of participants class satisfaction, self-directed learning ability and academic self-efficacy and it was identified that class satisfaction and self-directed learning ability affected academic self-efficacy. The findings of this study could be utilized as base data when developing a program to enhance nursing students self-directed learning ability and it is suggested that an interventional research of analyzing validity and effectiveness of developed education program would be also required.

중학교 과학교사들의 창조와 진화에 대한 관점연구

김세은(Se-Eun Kim),조정일(Jungil Cho) 전남대학교 종교문화연구소 2008 종교문화학보 Vol.5 No.-

우리나라의 과학과 교육과정에서는 생명과 종의 기원에 대한 내용이 진화론에 기초하여 다루어지고 있다. 현재 생명의 기원에 대한 논의가 계속되는 가운데, 학생들의 진화에 관련된 이론 수용에 많은 영향을 주는 중학교 과학교사들의 생명의 기원에 대한 관점을 알아보고자 하였다. 먼저 중학교 과학3의 진화단원의 내용을 중심으로 검사 도구를 만들었으며 광주광역시와 전라남도 시지역의 중학교 과학교사들을 상대로 질문지를 발송하여 회수된 115개의 질문지를 분석하였다. 중학교 과학교사들의 진화에 관련된 각 지식주장들에 대한 관점의 빈도와 평균값을 조사해본 결과 대체적으로 진화론적 관점과 반진화론적 관점 중 어느 한 쪽으로 쏠리지 않고 중간 값을 나타내었다. 지질시대와 화석 영역에서 가장 진화론적인 관점을 보여주었고, 시조새, 척추동물의 배 발생 과정, 돌연변이와 자연선택에 대해서는 대체로 진화론적인 관점을 보였다. 한편, 인류의 진화에 대해서는 대체로 반진화론적인 관점을 나타내었다. 중학교 과학교사들의 기원에 대한 관점은 종교별로 차이를 보였으나, 교사들이 진화단원을 가르칠 때 66%의 교사들이 교과서의 내용을 기초로 하되 진화의 한계와 그 대안인 창조론에 대해 소개하는 것으로 나타났다. 또한 52%의 과학교사들이 현재의 진화에 대한 교육 자료가 충분하지 않다고 생각하였으며, 47%의 과학교사들이 기원에 관한 수업을 준비할 때 창조론을 지도할 수 있는 교육 자료가 필요하다고 응답하였다. The purpose of this study is to find out viewpoints of junior high school science teachers on origins and teaching the topics. A questionnaire which was used in this study contained 32 questions about origins and 12 questions asking about teaching the topic. On those questions, teachers were asked to respond with 5 Likert-type responses. Questionnaires of a total of 115 teachers were analyzed. The total mean of 32 questions was 2.95, with the range of 2.5~3.4. The most evolutionary responses were obtained from questions about fossils and geological ages, and the relatively high evolutionary responses were obtained from questions about archaeopteryx fossil, homology, the mammal embryo development, mutation and natural selection. Whereas, teachers showed anti-evolutionary point of view on origin of human being. About 50 percent of teachers responded that they introduced alternative theory of origin and limit of evolutionary theory at the same time they taught the topic based on the curriculum. In addition, teachers responded that they wanted material developed from various perspectives on the origin of life. The results of the study suggested that alternative theory of origin may help teachers get better prepared to real classrooms consisting of students who had various point of views on the origin.

당뇨병 치료약제와 심혈관 질환

김세은 ( Se-eun Kim ),유병수 ( Byung-su Yoo ) 대한내과학회 2021 대한내과학회지 Vol.96 No.2

Diabetes is one of the important risk factors in cardiovascular disease associated with atherosclerosis, and cardiovascular disease is the leading cause of death in patients with diabetes mellitus. Recent randomized placebo-controlled cardiovascular outcome trials of all new antidiabetic drugs have linked SGLT-2 inhibitors and GLP1-agonists to not only increased cardiovascular stability but significant reduction of cardiovascular disease. These results have led to preferential selection of the most effective and beneficial antidiabetic drugs with the evidence of cardiovascular safety and efficacy. Herein, we address cardiovascular stability and the effectiveness of antidiabetic drugs, focusing on recently developed ones. (Korean J Med 2021;96:85-91)