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자극(刺鍼)이 난소(卵巢)를 절제(切除)한 흰쥐의 골밀도(骨密度)에 미치는 영향(影響)
김미려,양재하,Kim Mi-Ryeo,Yang Chae-Ha 대한침구의학회 1998 대한침구의학회지 Vol.15 No.2
Effects of acupuncture at Sanyinjiao(SP6) and Yanglingquan(GB34) on bone mineral sensity(BMD) was investigated in ovariectomized(OVX) rats. BMD of OVX rats was measured left femur and tibia 2 and 4 weeks after acupuncture stimulation, respectively. Compared with sham-operated rats, a significant decrease in spine, pelvic and left femoral and tibial BMD was observed 2 and 4 weeks after OVX, respectively. In contrast, a significant increase of spinal, pelvic and left femoral and tibial BMD was elicited 2 and/or 4 weeks after acupuncture stimulation, respectively. These results show the possibility that acupuncture can play a role of ameliorating osteoporosis by elevating serum levels of female hormones related with bone metabolism.
증례(證例) : 한방차의 여성 생리통 완화에 대한 임상체험 평가
김미려 ( Mi Ryeo Kim ) 대구한의대학교 제한동의학술원 2012 東西醫學 Vol.37 No.1
Objective: The object of this study was to investigate the efficacy of oriental herb tea in women with dysmenorrhea. Methods: 52 women with dysmenorrhea drank oriental herb tea twice daily for 1 month. The premenstrual symptoms and degree, VAS (Visual Analog Scale) and MVRS (Multidismensional Verbal Rating Scale) of dysmenorrhea was evaluated before and after treatment. And the Affinity of the product was investigated. Results: After treatment, premenstrual mood disorder, degree and duration time of dysmenorrhea and disorder of daily living and sleeping was reduced. And repurchase opinion of the product was 60.4%. Conclusion: These result suggest that drinking oriental herb tea is useful for improvement dysmenorrhea. Also it is needed the various attempts to change formulation, as well as development of herbal tea using medicinal herbs for reducing dysmenorrhea.
추출조건에 따른 차전초 추출물이 3T3-L1 세포의 지방 축적에 미치는 영향
정미진 ( Mi-jin Jeong ),김소영 ( So-young Kim ),김유진 ( Yoo-jin Kim ),이은탁 ( Eun-tag Lee ),추성태 ( Sung-tae Choo ),김한혁 ( Han-hyuk Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.2
Objectives : Plantaginis asiaticae Folium (PA) has been widely used in Korean medicine for treatment of liver disease, stomach troubles and inflammation. We investigated the effect of PA on lipid accumulation in 3T3-L1 according to extraction conditions. Methods : The MTT assay was employed to evaluate the cytotoxicity of PA extracted by different solvents (water, 50% ethanol, and 95% ethanol) on 3T3-L1 preadipocytes. Oil red O staining was used to identify intracellular lipid accumulation in 3T3-L1. 3T3-L1 adipocytes were treated with PA at concentration ranging in 0.1, 0.2 and 0.4 ㎎/㎖. PA was extracted by different extraction conditions such as extraction solvents, extraction time, and extraction temperature. In addition, UPLC analysis was used for determination of candidates of active ingredients in PA. Results : 3T3-L1 preadipocytes were treated with PA extracted by different solvents (water, 50% ethanol, and 95% ethanol) and there was no cytotoxicity. Oil red O staining was employed to identify the effect of PA on lipid accumulation in 3T3-L1. In the present study, PA water extraction at 70℃ for 6 hours decreased greatly in lipid accumulation. The range of concentrations was 0.1, 0.2 and 0.4 ㎎/㎖. Concentration at 0.2 ㎎/㎖ was the most effective one among them. Candidates of active ingredients in PA were shown plantamajoside and acteoside through UPLC. Conclusions : These results suggest that the effect of PA water extraction at 70℃ on lipid accumulation in 3T3-L1 is superior to other extraction conditions. We suppose that plantamajoside and acteoside may be candidates of active ingredients in PA.
차전초 잎의 물 추출물이 고지방식이로 비만 유도된 C57BL/6 생쥐의 체지방 감소에 미치는 영향
김소영 ( So Young Kim ),정미진 ( Mi Jin Jeong ),김유진 ( Yoo Jin Kim ),이은탁 ( Un-tak Lee ),추성태 ( Sung-tae Choo ),김한혁 ( Han-hyuk Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.2
Objective : Plantago asiatica L. has been used for many years in Korea, China, and Japan for the treatment of many diseases such as anti-inflammatory, anti-asthma and diuretic. But the anti-obesity effect of Plantaginis asiaticae Folium has not been studied yet. Thus, this study aimed to demonstrate the anti-obesity efficacy of Plantaginis asiaticae Folium water extract (PAF) in high fat diet-induced obesity animal model. Method : Male, five-weeks old C57BL/6 mice were divided into 5 groups; ND (normal diet + 0.9% saline), HFD (highfat diet + 0.9% saline), PC (high-fat diet + Garcinia cambogia 500 ㎎/㎏), PAF 100, 300 (high-fat diet + PAF 100, 300 ㎎/㎏. Treatments were performed daily for 6 weeks per os. We evaluated the changes of body weight, white adipose tissues weight, hepatic and fecal lipids level, pancreatic lipase activity and lipid profiles in plasma. Result : Body weights, adipose tissue weights and FER were significantly lower in mice fed the high-fat diet with PAF treatment than in mice fed the high-fat diet alone. In plasma, TG, TC, LDL-C, ATS and ALT levels significantly decreased in PAF groups compared with HFD group. Also, hepatic triglyceride (TG) levels decreased and fecal TG increased in PAF groups compared to HFD group. In addition, the activity of pancreatic lipase was decreased in the PAF groups compared to that of HFD group. Conclusion: These results suggest PAF extract is effective in preventing and improvement obesity, so it might be a potential and safe material for anti-obesity agent of weight control or functional supplements.
한약재 복합추출물이 모발 성장 및 멜라닌 생성 촉진에 미치는 영향
김지윤 ( Ji Yoon Kim ),김유진 ( Yoo-jin Kim ),김문주 ( Moon Ju Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.5
Objectives : This study was performed to determine the transdermal effects of ethanol extract from medicinal herbal mixture (SHJ) on hair growth in C57BL/6 mice and melanogenesis in melanoma cells. Methods : Mice were divided into 3 experimental groups including vehicle (CON), SHJ extract and 5% minoxidil (MNXD, positive control)-treated group. SHJ was applied topically on the hair-shaved skin of C57BL/6 mice everyday for 15 days. The thickness and density of hair with a folliscope and morphometry of hair follicle with a H&E staining were monitored at last day. Also then, hair growth-associated gene expressions were measured by immunoblot assay. Results : The MNXD or SHJ-treated group promoted on hair growth compared to that of vehicle-treated group (CON). Hair density and thickness of MNXD or SHJ treated-group increased compared to that of vehicle application on the 15 days, respectively. Induction of insulin-like growth factor (IGF)-1 and vascular endothelial growth factor (VEGF) were also accelerated by application of SHJ extract compared to those of CON group. But expression of transforming growth factor (TGF)-β1 decreased in SHJ treated-group compared to that of CON group. Furthermore, SHJ extract showed to increase melanin contents in a dose-dependent manner. Tyrosinase activity significantly increased in SHJ-treated group compared with CON group in dose-dependant manner. Conclusions : These results suggest that SHJ can be used as a component of cosmeceuticals for hair care via promoting growth and melanogenesis of hair.
차전초 잎의 물 추출물이 고지방식이로 비만이 유도된 C57BL/6 생쥐에서 지질대사 단백질 발현에 미치는 영향
김소영 ( So Young Kim ),정미진 ( Mi Jin Jeong ),김유진 ( Yoo Jin Kim ),이은탁 ( Un-tak Lee ),추성태 ( Sung-tae Choo ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.3
Objective : Previous studies showed that water extract of Plantago asiatica (Plantaginis asiaticae Folium, PAF) significantly controlled in body weights, adipose tissue weights and blood lipid profiles in obese C57BL/6 mice. To investigate the mechanism of anti-obesity action of PAF, expressions of obesity-related proteins were identified such as p-AMPK and p-ACC in hypothalamus, UCP-1 in brown adipose tissue, p-AMPK, p-ACC, SREBP-1c, PPARγ, HMGCR and CPT-1 in liver. Method : Five-weeks old male C57BL/6 mice were divided into 5 groups; ND (normal diet + 0.9% saline), HFD (high-fat diet + 0.9% saline), PC (high-fat diet+Garcinia cambogia 500 ㎎/㎏), PAF 100 and 300 (high-fat diet + PAF 100 or 300 ㎎/㎏). PAF was treated orally for 6 weeks. The protein expression of AMPK, p-AMPK, ACC, p-ACC, PPARγ, SREBP-1c, HMGCR, CPT-1 and UCP-1 were identified by expression levels of proteins through western blot analysis. Result : The results showed that protein expressions on hypothalamic p-AMPK and p-ACC did not differ between the HFD and PAF groups. In addition, PAF did not affect the increase of UCP-1 in brown adipose tissue. The protein expression levels of hepatic p-AMPK, p-ACC and CPT-1 increased in PAF groups compared to HFD group. And those of PPARγ, SREBP-1c and HMG-CoA decreased in PAF groups compared to HFD group. Conclusion : These results suggest that the PAF administration induce weight loss via inhibition of lipid metabolism-related protein expressions in hepatic tissues. Therefore, PAF could be used as a potent material of anti-obesity products for prevention and treatment of obesity.