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      • SCOPUSKCI등재후보

        공배양의 작용기전에 관한 연구

        김미경,주보선,김미선,문화숙,이규섭,김한도,Kim, Mi-Kyoung,Joo, Bo-Sun,Kim, Mi-Sun,Moon, Hwa-Sook,Lee, Kyu-Sup,Kim, Han-Do 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.1

        Objective: A number of studies to improve in vitro culture conditions have been tried over past ten years by using co-culture system with helper somatic cells. However, the mechanism of coculture is poorly understood. This study was designed to understand the mechanism for the mode of actual action of co-culture using co-culture system of ICR strain's 1-cell embryos with human oviduct epithelial cells by examining the effect of conditioned medium and contactless coculture using a cell culture insert on the embryo development and by measuring the level of superoxide anion from conditioned medium after co-culture. Methods: ICR strain's zygote embryos were cultured in medium alone (control), coculture, conditioned medium, or contactless coculture system for 6 days. Conditioned media (CM) were prepared as following 5 groups. All CM were collected after culturing oviduct cells for 2 days. CM-1 was stored at $-20^{\circ}C$ until use, and CM-2 was prepared just before use as a culture medium. CM-3 was cocultured with embryos and retrieved just before use. CM-4 and CM-5 were derives from the microfilteration of CM-2 and CM-3, respectively, using Microcon-10 (10 kDa molecular weight cut-off). The percentage of the embryos developed to hatched blastocyst stage and the level of superoxide anion in supernatant from medium alone culture (control), coculture, and contactless coculture were measured. Results: The rates of embryo development to the hatched blastocyst stage were significantly higher in coculture (43%) than in control (0%) (p<0.05). The CM-1 group had no embryo development since 2-cell embryonic stage, whereas the CM-2, CM-3, CM-4 and CM-5 groups had the improved development to 4 or 8 cell embryo stage, but the similar rate of development to hatched blastocyst compared to control. The effect of coculture on embryo development was disappeared in the contactless coculture group. The level of superoxide anion was significantly reduced in coculture group compared to control. Conclusion: It is concluded that the present coculture system overcomes the 2-cell block in vitro and improves the embryo development. This beneficial effect may be due to the direct cell-cell contact between embryo and helper cells or the removal of deleterious components from medium rather than the embryotrophic factors.

      • KCI등재

        일시적 거주개념을 적용한 1인용 소형주택의 공간구축유형 및 특성

        김미경,송애희,Kim, Mi-Kyoung,Song, Ae-Hee 한국주거학회 2012 한국주거학회 논문집 Vol.23 No.2

        The purpose of this study was to analyze the types and characteristics of space construction in temporary small-sized housing for single-person household through the understanding and interpretation of the modern flexible lifestyle. A document research method and case studies were used to analyze and classified the spatial characteristics of temporary dwelling spaces since 2000. Findings of the study were as follows: In order to conform the concept of temporary dwelling for small-sized housing units of single-person households, the types and characteristics of space construction were divided into three aspects: (1) 'Transporting' by wheel, rotation and vehicles, (2) 'Transforming' by adapting, assembling disassembling, and folding unfolding, (3) 'Wearing Carrying' by inflatable and tented type. In conclusion, this study found two types of space construction in temporary small-sized housing. The first was 'formal aspect' which was focused on the simplicity of shape, ease of deformation and lightweight of materials. And the second was 'functional aspect' which was focused on the complex space composition, the rapidity of installation and dismantling, ease of movement and transportation. This study shows that the combination of two types of the temporality will be more ideal in temporary small-sized housing planning rather than relying on just one type.

      • KCI등재

        현대집합주거계획에서 비정주성이 표현된 공간구성 특성에 관한 연구

        김미경,Kim, Mi-Kyoung 한국주거학회 2009 한국주거학회 논문집 Vol.20 No.4

        The purpose of this study is to analyze the characteristics of the non-sedentary in contemporary multi-housing planning through the understanding and interpretation of the modern nomadic life. A document research method was used to analyze and classified the spatial characteristics and development of the non-sedentary dwelling spaces since 1980. Finally, today the applicable cases of non-sedentary space are as follows; the sharing housing, the adapting housing, and temporary housing for the multi-purpose spaces, huge spaces and the industrialization housing. First of all, in order to conform the concept of nomadism, the theory and characteristics were divided into three aspects; The concept of thought of lines, events and Rhisome, and the spatial characteristics of fluidity, multiplicity and the non-hierarchy, that is mainly based on the philosophy of Gilles Deleuze. This study attempted to analyze how the concept of non-sedentary dwelling space has been developed in what form and method they have been applied and interpreted in the historical background. It attempted to resolve the concept of movement and mobility through the experiment so that they might apply to the space of contemporary city.

      • KCI등재

        단일 조성 실온 경화형 실리콘러버를 지지체로 사용한 고체상 탄산이온선택성 전극의 개발

        김미경,윤인준,조성호,신혜라,한종호,하정한,남학현,차근식,Kim, Mi-Kyoung,Yoon, In-Jun,Cho, Sung-Ho,Shin, Hye-Ra,Han, Jong-Ho,Ha, Jeong-Han,Nam, Hak-Hyun,Cha, Geun-Sig 대한화학회 2004 대한화학회지 Vol.48 No.3

        소형 고체상 전극에 단일 조성 실온 경화형(one-component room temperature vulcanizing type) 실리콘러버 730(730 RTV)을 지지체로 도입하여 가소제가 필요 없는 탄산이온선택성 전극막을 개발하였다. 본 연구에서 최적화된 탄산이온선택성 전극막의 조성은 85.75 wt%의 730 RTV, 11.08 wt%의 trifluoroacetyl-p-decylbenzene(TFADB), 3.17 wt%의 tridodecyl-methylammonium chloride(TDMACl)로 제조 되었고, 우수한 전기화학적 특성(감응기울기 (26.3 mV/dec.), 선택성($logKT^{pot}_{CO_{2},Cl^-}$= -4.00, $logKT^{pot}_{TCO_{2},Sal^-}$=1.69), 검출한계(4.0${\times}10^{-4}M$))을 보였으며, 이와 같은 조성의 막을 도입한 평면형 고체상 센서는 60일 이상 초기특성을 유지하였다. We developed a miniaturized solid-state carbonate ion-selective electrode (carbonate ISE) based on one-component room temperature vulcanizing type silicone rubber 730 (730 RTV) without adding plasticizer to the matrix. The optimized carbonate ion selective membrane is prepared with 85.8 wt% of 730 RTV, 11.1 wt% of trifluoroacetyl-p-decylbenzene (TFADB), and 3.1 wt% of tridodecyl-methylammonium chloride (TDMACl). This carbonate ISE exhibited excellent potentiometric properties (i.e., slope: 26.3 mV/dec; selectivity: $logKT^{pot}_{CO_{2},Cl^-}$= -4.00 and $logKT^{pot}_{TCO_{2},Sal^-}$=1.69); and detection limit for $TCO_2:\;4.0{\times}10^{-4}M$). In addition, the early potentiometric properties of the solid-state sensor with optimized membrane composition were not deteriorated for more than 60 days.

      • KCI등재

        유럽 컨테이너 건축물의 사례분석을 통한 국내 적용방안

        김미경,문영아,한수지,Kim, Mi-Kyoung,Mun, Young-A,Han, Su-Ji 한국주거학회 2015 한국주거학회 논문집 Vol.26 No.1

        The purpose of this study was to analyze the planning characteristics and it's application of container architecture as case study. Field survey was used to analyze the spatial planning characteristics in terms of development outline, appearance, exterior, floor plan and interior of eight famous cases in Berlin, Hamburg, Hannover, Amsterdam and Paris of Europe. The results of this study were as follows; Firstly, good examples of container architectures such as student housing, social service center, temporary medical facility and cruise terminal in Europe suggested the potential of domestic applicability in various purposes and development. Secondly, various types of freight container, building container and module frame system should be developed with their reprocessing environment. Thirdly, it is necessary for us to develop ISO type(20~40ft) container and standard plan with interior and storage design reflecting demands of residents. Finally, the use of container module will be an environmental-friendly alternative for its modularity and reusability, so it should be used as it is without severe deformation. The development of environmental friendly energy sources such as hydro and solar power is necessary for domestic container architecture as well. The container design should include the use of high quality of exterior finishing materials and the plan of aesthetical color planning to make the building a local landmark.

      • KCI등재후보

        집중력, 청각반응속도, 순발력이 민첩성에 미치는 영향

        김미경,남지윤,노수영,한동욱,Kim, Mi-Kyoung,Nam, Ji-Yoon,Noh, Su-Yeong,Han, Dong-Wook 대한물리치료과학회 2012 대한물리치료과학회지 Vol.19 No.3

        Background : The purpose of this study was to investigate the relationship between an agility and a concentration, an auditory reaction time and a muscle power. Methods : Twenty one healthy female college students who were 20-21 years participated in the experiment; after being introduced to the methods, they voluntarily signed a consent form. And they received several test related with this study; side step test for an agility, Harris & Harris test for a concentration, brain auditory evoked potential test for an auditory reaction time and vertical jump test for a muscle power. Result : There was no relationship between the agility and the concentration and the auditory reaction time. However, the muscle power related with the agility significantly. Conclusion : In conclusion, these findings suggest that a muscle power training is a good way to promote an agility. So, we can find out that, in an player who requires an agility, a muscle power training is the most important way for promoting an agility than a concentration training or an auditory reaction time training.

      • KCI등재

        최근 공동주택 설계경기에 나타난 단위세대 계획개념 및 공간계획 특성

        김미경(Kim, Mi-kyoung),문영아(Mun Young-a) 한국실내디자인학회 2009 한국실내디자인학회논문집 Vol.18 No.5

        The purpose of this study is to analyze the planning concept and spatial characteristics of the multi-housing units in prize-wining works of design competition since 2007. Literature review and content analysis were used for this study and 199units over 59㎡ were analyzed to review the planning concept, spatial composition and layout. The results of this study were as follows; 1)The planning concept of these units were divided into three aspects; that is, eco-friendly, rational and sustainable unit plan by means of functional, environmental-friendly and future-oriented aspects. 2)Most unit plans had 3LDK+2bath and 4Bay types including family room as a public space. This results shows that family-room and bathroom has recently become more important than number of rooms in multi-housing units planning. 2)In terms of the spatial composition of LDK and Master Bed room, LDK area become large, open type and master-zone were showed "bed room+dress and powder room+bath" type. 3)The entrance has a intermediated space as a balcony or a former space and the privacy of LDK space was protected from the entrance. This study has a significant meaning on grasping current trend in Korean multi-housing and to provide information for future direction on housing-unit design.

      • KCI등재

        색채프로그램과 예능프로그램을 활용한 대학생의 심리변화

        김미경(Mi-Kyoung Kim) 한국콘텐츠학회 2011 한국콘텐츠학회논문지 Vol.11 No.6

        본 연구는 예능프로그램을 시청한 후 색채프로그램을 실시한 실험집단과 실시하지 않은 통제집단의 심리상태(강박증, 불안 및 적대감)에 어떠한 영향을 미치는지 확인하기 위해 실시하였으며, 결과는 다음과 같다. 첫째, 색채프로그램에 참여한 실험집단의 심리상태(강박증, 불안 및 적대감)는 사전 검사치보다 사후 검사치 모두 95% 통계수준에서 유의적으로 낮아졌으나 통제집단을 그런 변화가 나타나지 않았다. 둘째, 예능프로그램을 시청한 후 색채프로그램을 참여한 대학생들의 색채표현에서 빨강색과 파랑색의 지각이 폭넓게 나타났다. 이러한 변화는 흰색의 이성과 검정의 억압에서 현재 해야 할 일에 대한 책임감을 갖는 변화과정이라고 해석할 수 있었다. 결론적으로, 예능프로그램을 시청한 대학생들의 심리상태에 긍정적인 영향을 줄 수 있도록 다양한 색채 프로그램을 교육과정에 도입하는 것은 의미 있다고 본다. This study will be conducted to verify that color programs and entertainment programs affect undergraduates' psychology(Obsessive-compulsive, anxiety and hostility). The results of this study can be summarized as follows. First, the experimental group’s psychology(Obsessive-compulsive, anxiety and hostility) participated in color programs than did all the pre-investigation levels decreased post-mortem examination, statistically significant at 95% appeared to be. Second, after watching entertainment programs, pass through cognition process of color, undergraduates' color representation worked perception of red and blue respectively. This results is that the reason of white and the suppression of black what to do now with a sense of responsibility can be considered as a change process. In conclusion, after watching entertainment programs take advantage of color programs that a positive impact on undergraduates’ psychological state, as they can provide bright colors with a variety of ways that make sense to suggest.

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