식방풍의 성분분리 및 생리활성

김도훈,한지수,김기은,김진효,김성건,김호경,오오진,황완균,Kim, Do-Hoon,Han, Chi-Su,Kim, Gi-Eun,Kim, Jin-Hyo,Kim, Sung-Gun,Kim, Ho-Kyoung,Oh, O-Jin,Whang, Wan-Kyunn 대한약학회 2009 약학회지 Vol.53 No.3

In this study, isolation of antioxidative compounds was performed for development of anti-oxidizing agent. $CHCl_3$, $H_2O$, 30%, 60% MeOH, MeOH fractions were examined antioxidative activity by DPPH, test of inhibition on NO production. It was revealed that 30%, 60% MeOH and $CHCl_3$ fractions had significant antioxidative activity. In 30% MeOH and 60% MeOH, $CHCl_3$ fraction, six compounds were isolated and elucidated as adenosine(I), guanosine(II), peucedanol 7-O-$\beta$-D-apiofuranosyl(1$\rightarrow$6)-$\beta$-glucopyranoside(III), peucedanol 7-O-$\beta$-D-glucopyranoside(IV), peucedanol(V) and scopoletin(VI) by physicochemical data and spectroscopic methods. (Negative FAB-MS, $^{1}H-NMR$, $^{13}C-NMR$). The results from antioxidative activity screening for the each compound showed that compound IV was relatively superior antioxidant ability. In anti-inflammatory activation assay, compound III, IV, VI had concentration-dependent-activity and compound IV had superior anti-inflammatory ability. These results suggest that Peucedani Radix might be developed as a potent anti-oxidative, anti-inflammatory agents and ingredients for related functional foods.

조현병 환자와 발병하지 않은 일차친족에서 신경인지의 결함

김도훈,김지우,황선영,김병수,원승희,Kim, DoHoon,Kim, Jiwoo,Hwang, Sunyoung,Kim, Byungsoo,Won, Seunghee 대한생물정신의학회 2014 생물정신의학 Vol.21 No.2

Objectives This study aimed to identify the differences and the profiles of cognitive deficits in remitted patients with schizophrenia and first-degree relatives of schizophrenic probands. Methods A total of 26 remitted states of schizophrenia patients were included in the study and the same number of unaffected first-degree relatives of schizophrenic probands and healthy controls were matched for age, sex, years of education. Cognitive function of all participants was measured by using the Digit span test, the Continuous performance test, the Rey auditory & visual learning test, the Complex figure test, the Verbal fluency test, the Wisconsin card sorting test and the Finger tapping test. The effects of subsyndromal symptomatology and general intelligence score were controlled. Results Schizophrenia patients' group showed more significant impairment than other groups in verbal memory (learning, immediate recall, delayed recall), visual memory (copy, immediate recall, delayed recall) and cognitive flexibility domains. The family group and the patient group commonly performed significantly worse than healthy controls in working memory and verbal fluency (category) tests. There were no differences in sustained attention, psychomotor performance. Conclusions Our research shows that the deficit in working memory and verbal fluency could be strong candidates of endophenotypic marker in schizophrenia.

RAPD를 이용한 들깨 유전자원의 유전적 변이 분석

김도훈,양보경,김현경,김나영,정순재,김익수,남재성,이재헌,정대수,Kim, Doh-Hoon,Yang, Bo-Kyung,Kim, Hyeon-Kyoung,Kim, Na-Young,Jeong, Soon-Jae,Kim, Ik-Soo,Nam, Jae-Sung,Lee, Jai-Heon,Chung, Dae-Soo 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3

Genetic variation of Perilla germplasms was investigated using RAPD markers. Forty-two Perilla frutescens lines and cultivars collected form locals were subjected to RAPD analysis using 220 primers. Among them only 13 primers showed polymorphic bands and these 13 primers provided a total of 144 bands, consist of 115 polymorphic and 29 monomorphic ones. The polymorphic bands were subjected to phylogenetic analysis using UPGMA and maximum parsimony (MP) methods. In the UPGMA method, similarity coefficiency of 42 Perilla frutescens lines and cultivars ranged from 0 to 0.7842. The dendrogram of 42 lines and cultivars obtained through UPGMA method resulted in two major groups, and the similar clustering pattern was found by MP method, suggesting Perilla germplasms utilized in this study truly can be divided into two major groups. Although the two major groups were consistent roughly with their phenotypes (under of node, weight of 1,000 grains, and oil content), in detail, much inconsistency also was present.

2022 동일건축 대학(원)생 설계 VE 경진대회 참관기

김도훈,김용찬,박규호,이정재,황태웅,박민수,김세영,권단비,Kim, Do-Hun,Kim, Yong-Chan,Park, Gyu-Ho,Lee, Jeong-Jae,Hwang, Tae-Ung,Park, Min-Su,Kim, Se-Yeong,Gwon, Dan-Bi 한국건설관리학회 2022 건설관리 : 한국건설관리학회 학회지 Vol.23 No.5

c-$\phi$ 지반에서의 아칭현상을 고려한 원형수직터널 토압 : II. 실내 모형실험

김도훈,차민혁,이대수,김경렬,이인모,Kim, Do-Hoon,Cha, Min-Hyuck,Lee, Dea-Su,Kim, Kyung-Ryeol,Lee, In-Mo 한국터널지하공간학회 2010 한국터널지하공간학회논문집 Vol.12 No.2

원형수직터널에 작용하는 토압은 아칭효과로 인해 2차원 일반 흙막이벽에 작용하는 토압보다 작으므로 원형수직터널 설계 시 벽체에 작용하는 실질적인 토압의 예측이 필요하다. 본 논문은 두 개의 연속된 논문(Companion papers)의 두번째로서 원형수직터널 설계 시 건조한 사질토뿐만 아니라 c-$\phi$ 지반과 다층지반에서 적용 가능하도록 새롭게 제안된 토압식(김도훈 등, 2009)을 증명하기 위해 대형 모형실험을 수행하였다. 고안된 모형실험 장비는 단계별 굴착이 가능하도록 제작 벽체의 반경을 변화시켜가며 실험을 수행하였다. 또한 강사 방법으로 지반을 조성하기 전 건조한 시료에 물을 첨가하고 불포화사질토를 형성시켜 겉보기 점착력을 발현시킴으로써 c-$\phi$ 지반과 다층지반에서 실험을 진행하였다. 실험 결과로서, 단계별 굴착을 모사하였을 때, 아칭효과에 의해 굴착된 지반에서 굴착되지 않은 지반으로 하중이 전이가 일어나는 확인할 수 있었다. 또한, 동시에 굴착했을 때의 토압은 예측한 값에 비해 상당히 작게 나타났지만, 단계별로 굴착했을 때의 최종 토압은 동시 굴착 시의 토압에 비해 크게 나타나며 새롭게 제안된 토압식과 잘 일치하였고 c-$\phi$ 지반과 다층지반에서 수행한 실험의 결과도 겉보기 점착력의 효과로 인해 토압의 감소를 보이며 이론적인 값과 잘 일치하는 것으로 나타났다. The earth pressure acting on the vertical shaft is less than that acting on the retaining wall due to three dimensional arching effect. Thus, it might be essential to estimate the earth pressure actually acting on the shaft when designing the vertical shaft. In this paper, large-sized model tests were conducted as Part II of companion papers to verify the newly suggested earth pressure equation proposed by Kim et al. (2009: Part I of companion papers) that can be used when designing the vertical shaft in cohesionless soils as well as in c-$\phi$ soils and multi-layered soils. The newly developed model test apparatus was designed to be able to simulate staged shaft excavation. Model tests were performed by varying the radius of vertical shaft in dry soil. Moreover, tests on c-$\phi$ soils and on multi-layered soils were also performed; in order to induce apparent cohesion to the cohesionless soil, we add some water to the dry soil to make the soil partially-saturated before depositing by raining method. Experimental results showed a load transfer from excavated ground to non-excavated zone below dredging level due to arching effect when simulating staged excavation. It was also found that measured earth pressure was far smaller than estimated if excavation is done at once; the final earth pressure measured after performing staged excavation was larger and matched with that estimated from the newly proposed equation. Measured results in c-$\phi$ soils and in multi-layered soils showed reduction in earth pressures due to apparent cohesion effect and showed good matches with analytical results.

침투를 고려한 원형수직터널 거동특성 연구

김도훈,이강현,이인모,Kim, Do-Hoon,Lee, Kang-Hyun,Lee, In-Mo 한국터널지하공간학회 2011 한국터널지하공간학회논문집 Vol.13 No.6

원형수직터널의 시공시 지하수가 존재하는 경우 굴착에 의해 지하수의 흐름이 발생되어 추가적인 힘이 작용하게 된다. 원형수직터널의 지하수의 흐름은 일반적인 수평터널과 달리 깊이방향으로 다르게 작용되며, 수직터널의 벽체에 경사방향으로 작용하게 된다. 본 연구에서는 원형수직터널에 작용하는 침투력의 영향을 파악하기 위해 깊이에 따라 변하는 경사방향의 흐름성분을 수직과 수평방향으로 나누어 각각을 이론식에 적용하였다. 지하수가 영향을 미치는 범위는 토압을 야기하는 이완영역과 같은 것으로 가정하여 이론식을 유도하였다. 침투력에 의해 발생되는 추가적인 힘으로 인하여 원형수직터널의 이완영역에 작용되는 응력이 달라짐에 따라 침투력을 고려한 토압계수 산정식과 원형수직터널 벽체에 작용하는 응력 산정식을 본 연구를 통해 제안하였다. 가상지반을 설정하고 제안식을 적용한 결과, 침투를 고려하지 않은 건조한 지반에 비하여 수직방향응력은 약 1.4배, 토압은 2.5배 증가하는 결과를 보였다. 침투해석을 통하여 "유효응력+침투력"으로 구한 값과 제안식을 이용하여 산정한 값은 유사한 경향을 보여 제안식은 침투력을 적절히 고려하여 토압을 예측하는 것으로 나타났다. When a circular vertical shaft is constructed below the groundwater level, additional forces caused by groundwater flow besides horizontal effective stresses will act on the wall. The inward direction of the groundwater flow will be inclined to the vertical wall and its direction will change depending on the wall depth. In this paper, to figure out the effect of seepage forces acting on the circular vertical shaft, the slope of the inclined flow varying with the depth is divided into vertical and horizontal components to derive the coefficient of earth pressure considering the seepage pressure and to obtain the vertical stress by taking the seepage pressure into account. The control volume in this study is assumed to be the same with that of the dry ground condition within which the earth pressure is acting on the wall by the creation of the plastic zone during shaft excavation. An example study shows that the vertical stress increases by about 1.4 times and the horizontal earth pressure increases up to 2.5 times compared to the dry ground condition. The estimated values from the proposed equation considering seepage forces and the calculated values from numerical analysis with "effective stress plus seepage force" show similar values, which verifies appropriateness of the proposed equation to estimate the earth pressure under the seepage condition.

정신신경면역학 개관

김도훈,Kim, Do Hoon 대한생물정신의학회 2008 생물정신의학 Vol.15 No.3

This review briefly summarizes the relevant knowledge of psychoneuroimmunological basis for neuroimmunology, with particular emphasis on bidirectional neural-immune interactions. The immune system and the nervous system maintain extensive communication, including hardwiring of sympathetic and parasympathetic nerves to lymphoid organs. Immune system is modulated by various neurotransmitters such as acetylcholine, norepinephrine, substance P and histamine. Neuroendocrine hormones such as corticotrophin-releasing hormone(CRH) or substance P regulate cytokine balance. The immune system modulates brain activity including sleep and body temperature. Recent studies have revealed that psychological factors which influence immunity and immune-related disease may modulate brain-to -immune interaction. But, we still await the scientific research and evidences to prove whether or how behavioral or treatment intervention of stress can influence the development, progress or prevention of a specific disease.

도시부 교차로에서의 자전거 사고유형 분석에 관한 연구

김도훈,조한선,김응철,Kim, Do-Hoon,Cho, Han-Seon,Kim, Eung-Cheol 한국도로학회 2008 한국도로학회논문집 Vol.10 No.4

최근 녹색교통수단으로서 자전거의 이용자수는 급격하게 증가하고 있으나 차대 자전거사고 감소와 자전거 이용자의 안전성 향상에 대한 노력은 미비한 설정이다. 따라서 본 연구에서는 최적의 교차로 설계지침 제공 및 자전거 사고유형에 영향을 미치는 요인들을 면밀히 분석하여 교차로에서의 자전거사고 안전성 향상에 그 목적이 있다. 이를 위해 본 연구에서는 2005년도 인천광역시 사지교차로에서 발생한 56건의 자전거사고 자료에 대한 분석과 사고발생 교차로에 대한 현장조사를 실시하였으며, 다항로짓모형을 이용하여 3가지 경우에 대한 자전거 사고유형 분석모형을 개발하였다. 모형분석결과, 사망사고 유무, 부도로 교통섬 유무, 도로위계, 사고당시 날씨, 주도로 버스정류장 유무, 주도로 차로폭, 인적유발요인이 자전거 사고 유형에 중요한 변수로 나타났다. Recently, users of bicycles as a green mode are rising sharply, but there are few efforts to increase bicycle safety and to decrease the number of accidents between vehicles and bicycle users. Therefore, this research seeks to improve bicycle safety at intersections by analysing causation factors of bicycle accidents pattern and providing optimal intersection design guidelines. For this study, real bicycle accident data of fifty six occurred in the Incheon metropolitan area in the year of 2005 were collected and field surveys were conducted. In addition, this research developed a bicycle accident pattern model with using multinomial logit model. The model results showed that presence of fatality, presence of traffic islands on the minor road, highway_type, weather, presence of traffic bus_stop on the major road, minor road lane width, victim induce factor are significant for bicycle safety.

유통 한약재 중 내분비계 장애물질로서의 잔류농약에 관한 연구(I)

김도훈,김혜수,오미현,강인호,심영훈,황완균,명승운,최병기,조정희,Kim, Do-Hoon,Kim, Hye-Soo,Oh, Mi-Hyune,Kang, In-Ho,Shim, Young-Hun,Hwang, Wan-Kyun,Myung, Seong-Wun,Choe, Byung-Ki,Cho, Jung-Hee 한국생약학회 2000 생약학회지 Vol.31 No.4

Research on pesticide residues as endocrine disruptors in natural medicines was initiated by Korea Food & Drug Administration this year. We determined the presence and levels of certain pesticides in selected natural medicines. The natural medicines collected this year are Glycyrrhiza Root, Cinnamon Bark, Pueraria Root, Polygonatum Rhizome, Jujube, Schizandra Fruit, Lycium Fruit, Liriope Tuber, Eucommia Bark, Peony Root, Korean Angelica, Dioscorea Rhizome, Cnidium Rhizome, Cassia Seed, Platycodon Root, Comus Fruit, Mentha Herb, Epimedium Herb, Bupleurum Root, and Ginger, which have no data for pesticide residues and 192 samples of them were circulated in Korea, 28 samples were circulated in China. In order to analyze many pesticides in large number of samples we used simultaneous multi-residue analysis of pesticides by GC-ECD, which was followed by GC-MSD analysis to confirm the identity of the detected pesticide in each sample.

c-${\phi}$ 지반에서의 아칭현상을 고려한 원형수직터널 토압: I. 이론

김도훈,이대수,김경렬,이용희,이인모,Kim, Do-Hoon,Lee, Dea-Su,Kim, Kyung-Ryeol,Lee, Yong-Hee,Lee, In-Mo 한국터널지하공간학회 2009 한국터널지하공간학회논문집 Vol.11 No.2

원형수직터널에서 3차원적인 아칭효과를 고려한 토압산정을 위해 여러 연구가 수행되었고 실내시험 및 수치해석을 통해 이를 검증하였으나, 다층지반과 c-${\phi}$지반에서의 적용이 어려웠다. 본연구에서는 c-${\phi}$지반에서의 토압 산정을 위해 c-${\phi}$지반에서 적용 가능한 토압계수 산정식을 구하였으며, 기존 토압식을 수정 제안하였다. 점착력이 토압에 미치는 영향을 파악하기 위해 지반을 가정하여 각 경우별로 토압을 산정하여 비교하였으며, 다층지반에서 파괴면을 가정하는 방법으로 토압을 구하였다. 이 논문은 두 개의 연속된 논문(Companion paper)의 첫 번째로서 모델개발을 위한 이론전개를 다루고 있으며, 대형 모형실험에 의한 실증은 두 번째 논문에서 다룰 것이다. Several researches have been done to estimate the earth pressure on a vertical circular shaft considering three dimensional arching effect and verified them by conducting model tests. However, any equation suggested so far is not applicable in case of multi-layered soils and/or C-${\phi}$ soils. In this study, new equation for estimating the earth pressure acting on the vertical shaft in c-${\phi}$ soils is proposed. A parametric study is performed to investigate the significance of the cohesion when estimating the coefficient of earth pressure in C-${\phi}$ soils and estimating earth pressures in vertical shafts. A method which can estimate the earth pressure on vertical shafts in layered soils is also proposed by assuming a failure surface in layered soils and using the modified equation. This paper is Part I of companion papers focusing on the theoretical aspect of model developments; the experimental verification will be made in Part II.