Tc-99m-MAA 를 이용한 간세포암의 경동맥 관류스캔

김강득,손광준,민경윤,권영미,김창근,노병석,원종진 ( Gang Deuk Kim,Kwang Joon Sohn,Kyung Yoon Min,Young Mi Kwon,Chang Guhn Kim,Byung Suk Noh,Jong Jin Won ) 대한핵의학회 1994 핵의학 분자영상 Vol.28 No.3

Puryose : Hepatic arterial perfusion scintigraphy with Tc 99m macroggregated albumin (HAPS) study was carried out in 16 patients with hepatocellular carcinoma(HCC) and in six patients without liver tumor to evaluate HAPS findings of hepatocellular carcinoma and usefullness of HAPS, Materials and Methods : HAPS with planar and SPECT study were performed in 22 patients after conventional hepatic or celiac arteriography. For HAPS study, 4 5 mCi of MAA mixed with 2ml of saline was injected into proper hepatic artery or its distal branches at the rate of approximately 1ml/sec. We anatysed 21 HCCs over 2cm in diameter(average diameter : 6.4cm) and 17 of 21 HCCs were over 4cm in diameter(Table 1). CT, sonography and angiography were performed within two week in all 16 patients and liver scan was performed in 12 patients. Results : Three different pattern of tumor perfusion were observed in 16 pat.ients with HCC (Table 2), 1) diffuse increased perfusion in 16 of 21(76%)(Fig. 1) 2) increased peripheral perfusion in 4 of 21(19%)(Fig. 2) 3) diffuse decreased perfusion in 1 of 21(5%) Arteriovenous shunt indicated by lung uptake of MAA were observed in 9 of 16(56% ) (Fig. 4). In contrast, angiography demonstrates arteriovenous shunt in 2 of 16(13%). There was no accumulation of radioactivity on RRC blood pool scan in all six patients with HCC examined (Fig. 1). Conclusion : HAPS is useful study in evaluation of perfusion pattern or vascularity of HCC and in detection of arteriovenous shunt.

자궁경부암 세포에 시스플라틴이 소포체 스트레스에 미치는 영향

김훈영 ( Hun Young Kim ),김병륜 ( Byoung Ryun Kim ),김강득 ( Gang Deuk Kim ),김형진 ( Hyung Jin Kim ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.4

목적: 항암제 시스플라틴의 세포독성 기전은 활성산소종과 lipid peroxydation의 증가와 DNA adduct의 형성 등이 알려져 있다. 이 연구에서는 시스플라틴이 자궁경부암 세포인 Hela 세포에서 과량의 NO와 세포 내 칼슘 이온의 농도 증가를 통해 소포체 스트레스 관련 단백질들의 발현을 증가시킴을 확인하고자 하였다. 연구방법: Hela세포는 시스플라틴으로 처리하였고 다음으로 소포체 스트레스 반응은 western blot, Flow cytometry, fluorescence 분석을 이용하여 측정하였다. 결과: 시스플라틴에 의해 유도되어지는 세포자멸사 현상은 유도성 NO 합성효소의 특이적 억제제인 1400 W와 세포 내 칼슘이온 소거제인 1, 2-bis-(o-aminophenoxy)ethane-N,N,N`,N`-tetraacetic acid tetra-(acetoxymethyl) ester에 의해 감소되었다. 이러한 약물들은 또한 미토콘드리아의 막전위를 회복시킴으로써 미토콘드리아에 의한 세포자멸사 신호전달은 억제하였다. 결론: 시스플라틴에 의한 세포자멸사 현상은 초기 시간대의 칼슘이온 농도의 증가와 그에 따른 미토콘드리아에 의한 세포자멸사 신호전달체계에 의해 유도되며, 이때 소포체가 중요하게 관여되어 있음을 확인할 수 있었다. Objective: Cis-diamminedichloroplatinum (cisplatin) is a widely used chemotherapeutic agent. A number of evidences in cytotoxic mechanism of cisplatin, including perturbation of redox status, increase in lipid peroxydation, formation of DNA adduct, have been suggested. The author hypothesized that cisplatin would mediate apoptosis via endoplasmic reticulum (ER) stress in human cervical cancer cell. Methods: Human cervical cancer cell line (Hela cells) were treated with cisplatin and then ER stress-related response were performed using western blot, Flow cytometry and fl uorescence analysis. Results: After addition of cisplatin to Hela cells, the author observed an expression of ER stress response genes through a gradual increase of nitric oxide and cytosolic Ca2+ concentration. Cisplatin-induced apoptosis can be inhibited by the inducible nitric oxide synthase inhibitor, 1400 W, and intracellular Ca2+ chelator, 1, 2-bis-(o-aminophenoxy)ethane-N,N,N`,N`-tetraacetic acid tetra-(acetoxymethyl) ester (BAPTA-AM). These inhibitors also reduced mitochondrial apoptotic signals, such as mitochondrion membrane potential disruption, cytochrome c release and eventually reduced the death of Hela cells. Conclusion Taken together, ER would seem to contribute to cisplatin-induced apoptosis via both the early release of Ca2+ and the late amplifi cation of mitochondria-mediated apoptotic signals.

Rebamipide Protects Colonic Damage Induced by Trinitrobenzene Sulfonic Acid (TNBS) via Down-Regulation of TNF-α, IL-1β‚ and ICAM-1

김연하(Yeun-Ha Kim),김성중(Sung-Jung Kim),황호근(Ho-Geun Hwang),최석채(Suk-Chei Choi),김유림(Yu-Rim Kim),김경숙(Kyung-Suk Kim),김철(Chul Kim),최덕화(Deok-Hwa Choi),오정택(Jung-Taek Oh),김강득(Gang-Deuk Kim),김혜원(Hye-Won Kim),전창덕(C 대한해부학회 2004 Anatomy & Cell Biology Vol.37 No.2

염증성 장 질환의 병리기전에는 tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) 및 intracellular adhesion molecule-1 (ICAM-1)의 발현 등 수많은 염증반응의 매개 물질들이 관여하고 있다. Rebamipide는 위염과 위궤양 치료제로 개발된 약제로 아직까지 염증성 장 질환의 치료효과를 검증한 연구는 거의 없다. 따라서 본 연구에서는 Rebamipide의 염증성 장 질환에 대한 치료효과를 검증하고 그 기전을 분석하고자 Rebamipide의 TNF-α와 IL-1β, 그리고 세포의 부착에 중요한 역할을 하는 ICAM-1의 발현의 조절에 대한 효과를 조사하였다. 5%의 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)를 실험 첫째날과 7일째에 직장 내로 넣어줌으로써 염증성 장질환의 동물모델을 제작하였고 2mM의 Rebamipide를 실험 0일, 3일, 6일째에 역시 직장 내로 투여하여 염증 억제 효과를 분석하였다. Rebamipide는 TNBS에 의하여 유도되는 체중감소를 현저히 억제하였으며 대장의 충혈과 부종역시 억제하였다. 조직소견에서도 Rebamipide 는 TNBS에 의해 유도되는 염증세포의 침착을 현저히 억제하였다. 또한 Rebamipide에 의해 TNF-α, IL-1β 및 ICAM-1의 발현 역시 현저히 억제되는 것을 면역 조직 화학 염색법을 통하여 확인하였다. 결론적으로 Rebamipide는 TNF-α, IL-1β와, ICAM-1의 발현을 조절함으로써 TNBS에 의해 유도되는 대장의 손상을 억제하리라고 생각되며 염증성 장 질환의 치료에도 좋은 효과가 있을 것으로 생각한다. During inflammation of the colon, cells of the gut mucosa produce or express numerous inflammatory mediators, such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and intercellular adhesion molecule 1 (ICAM-1). These mediators have been implicated as contributory factors to the inflammatory process, which results in colitis during inflammatory bowel disease (IBD). Rebamipide is an anti-gastric ulcer drug with anti-inflammatory properties in vivo and in vitro. The effects of Rebamipide on IBD have not been largely evaluated. Therefore, this study investigated the potential of Rebamipide to regulate the production of inflammatory mediators such as TNF-α, IL-1β, and ICAM-1. Mice with trinitrobenzene sulfonic acid (TNBS)-induced colitis (IBD animal model), were treated intrarectally with 2 mM Rebamipide. Body weight, macro- and micro-histological scores, and activity were evaluated. As an index of tissue edema, the thickness of the colonic wall was measured between the serosal surface and the luminal surface of the mucosa. TNF-α, IL-1β, and ICAM-1 were detected by immunohistochemical staining. Rebamipide treatment of mice exhibiting TNBS-induced colitis dramatically improved the clinical and histopathological findings of inflammation. In addition, Rebamipide suppressed TNF-α, IL-1β, and ICAM-1 expression in TNBS-treated animals. Taken together, these findings suggest that Rebamipide is a potential therapeutic agent for treating patients with IBD.

골프채 파악에 의한 제3 구역 내 심 수지 굴곡건 폐쇄성 파열

김영진 ( Yeung Jin Kim ),김태균 ( Tae Kyun Kim ),양환덕 ( Hwan Deok Yang ),김형준 ( Hyoung Jun Kim ),채수욱 ( Soo Uk Chae ),김강득 ( Gang Deuk Kim ),이지완 ( Ji Wan Lee ) 대한스포츠의학회 2009 대한스포츠의학회지 Vol.27 No.1

Closed rupture of flexor tendons without an associated pathologic condition in the hand is rare. Authors report a patients who sustained a rupture of flexor digitorum profundus in Zone III on his left little finger as a result of an repeated inappropriate strong grip of a golf club. The patient had the good range of motion with excellent clinical results after direct repair by modified Kessler method.

족무지 지관절 종자골에 의한 난치성 족저 각화증 (증례보고)

채수욱,김강득,김종윤,차명수,Chae, Soo Uk,Kim, Gang Deuk,Kim, Jong Yun,Cha, Myoung Soo 대한족부족관절학회 2013 대한족부족관절학회지 Vol.17 No.3

A sesamoid bone can occasionally be found under the interphalangeal joint of the hallux. These had anatomical variants and usually remain asymptomatic, it is uncommon for symptomatic cases of painful plantar keratoses or irreducible dislocation of interphalangeal joint of the hallux with incarcerated sesamoid. While the latter has a few cases, the former has not reported in Korea. We experienced a rare case of intractable plantar keratoses due to interphalangeal sesamoid bone of the hallux which may reqire excision.

버킷 림프종의 세침흡인 세포소견 -짧은 증례 보고-

조향정,오정택,김강득,김헌수,Jo, Hyang-Jeong,Oh, Jung-Taek,Kim, Gang-Deuk,Kim, Hun-Soo 대한세포병리학회 2007 대한세포병리학회지 Vol.18 No.2

A case of Burkitt lymphoma diagnosed through fine needle aspiration cytology is described, A 66-year-old man presented with lymphadenopathy on the right cervical neck and axillary area. An ultrasonogram and CT scan for the cervical neck showed a multiple variable sized lymphadenopathy with pericapsular invasion and necrotic foci, Fine needle aspiration cytology (FNAC) of the cervical lymph nodes revealed very cellular and necrotic smears consisting of diffusely scattered intermediate monotonous round cells, tingible body macrophages and many mitotic figures. Histological features of the excised lymph nodes showed the classic morphological features of Burkitt lymphoma. Recognition of the characteristic cytological features can suggest the possibility of Burkitt lymphoma.

유소아기의 급성 우하복부통증의 드문 원인 정상 자궁부속기의 염전

이승필 ( Seung Pil Lee ),김강득 ( Gang Deuk Kim ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.2

흰쥐 신경교종세포에서 카드뮴 세포독성에 대한 키토산의 효과

백용아(Yong-Ah Baik),이정래(Jeoung-Rae Lee),김강득(Gang-Deuk Kim),김혜원(Hye-Won Kim),이한솔(Han-Sol Lee),허정무(Jung-Mu Hur),오재민(Jay-Min Oh),최민규(Min-Kyu Choi),정연태(Yeun-Tai Chung) 한국독성학회 2004 Toxicological Research Vol.20 No.1

Casapse-3 protease is known as a key role of apoptotic enzyme, and caspase-3 activity is a central event that occurs upstream of DNA fragmentation during apoptosis. This study demonstrates that chitosan pretreatment inhibits cadmium-induced apoptosis by attenuating the activity of caspase-3. We also analyzed the protective effect of chitosan on DNA fragmentation induced by cadmium. Cadmium toxicity was examined by DNA fragmentation and nuclear condensation with Hoechst stain. Caspase-3 activities were increased cadmium treated group for 3 hours compared with control. When chitosan (150 mg/ml) was pretreated at 30 min before cadmium treatment, cadmium cytotoxicity was suppressed in a dose-dependent manner evaluated by DNA fragmentation and caspase activity. From these results, it is suggest that the protective effect of chitosan pretreatment against cadmium-induced cytotoxicity is mediated through inhibition of caspase-3 protease activation and DNA fragmentation.

복부결핵의 지연진단으로 인하여 발생한 복막농양의 경피적 배액술

김승호,오정택,김강득,위대한 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

Abdominal tuberculosis is described in two forms; tuberculous peritonitis and gastrointestinal tuberculosis. Abdominal tuberculosis is usually insidious onset with diverse symptoms and signs. The diagnosis is difficult, especially in areas where the disease is less common, as many patients do not have evidence of pulmonary tuberculosis or a positive skin test. Physicians should obtain a thorough history focusing on risk behaviors for tuberculosis. Delayed diagnosis is common, resulting in high mortality. In general, the same regimens are used to treat pulmonary and abdominal tuberculosis. Most intra-abdominal and other types of fluid collections are now successfully drained percutaneously under image guidance. The utility of percutaneous drainage(PCD) of tuberculous abscesses is less well established. A 43-years-old man, who had been diagnosed as pulmonary tuberculosis about 9 years ago, suffered from peritoneal abscess because of delayed diagnosis. The diagnosis of tuberculosis was confirmed by the PCR tests for the detection of Mycobacterium tuberculosis in the aspirates from the abscess. Drainage was done by catheter under ultrasound guidance and there were no procedural complications. The abscess was successfully and completely evacuated. We present our experience with a case of tuberculous peritoneal abscess which were successfully treated by PCD and antituberculous medication.

16채널 다중검출 CT를 이용한 말초 폐동맥의 평가

김강득,오정택 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2