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실내 수조 실험에서 은신처 형태에 따른 해삼 (Apostichopus japonicus, Selenka)의 은신처 선호도
권인영(Inyeong KWON),문선주(Sunju MOON),김재호(Jaeho KIM),이석화(Seokhwa LEE),임규환(Kyuhwan LIM),김태호(Taeho KIM) 전남대학교 어업기술연구소 2016 어업기술연구소보고지 Vol.9 No.1
Sea cucumber aquaculture has increased in the last several years due to the exploitation and population depletion of natural stocks. To investigate product potential, this study was conducted to examine several physical aspects of shelter design necessary for enhancing sea cucumber resources. Physical factors were restricted to hole shape (i.e., circle, triangle and quadrangle) of the shelter structure focused on which hole shapes sea cucumber prefer by water temperature (10, 15, 20℃). Experiments were conducted in a recirculation aquaculture system with dimensions of 2100 × 1000 × 1100 mm. Observations of video camera footage were made of sea cucumber given the choice of experimental plastic shelters with circular, triangular, and quadrangle holes. Sea cucumber used in the experiment was 13.38 ± 2 g in body weight. Fifty sea cucumbers were used for each experiment, based on the maximum inner area of all holes capable of occupation. The same experiment was replicated 3 times. Sea cucumbers were placed at distances of about 450 mm from the blocks. The data were evaluated by an analysis of variance (ANOVA). More sea cucumbers were attracted to circular holes than others in the experiment plastic shelters at 15℃ and 20℃ (P<0.05).
유수식 수조 실험을 통한 통합형과 은신처형 해삼(Apoctichopus japonicus, selenka) 양성기구의 생물학적 성능 평가
권인영(Inyeong Kwon),정지훈(Ji-Hoon Jung),이효영(Hyo-Yong Lee),문선주(Sun-Joo Moon),오무환(Moo-Hwan Oh),김태호(Taeho Kim) 전남대학교 어업기술연구소 2014 어업기술연구소보고지 Vol.7 No.1
A series of experiments were conducted to evaluate biological performances of three types of rearing systems for sea cucumber grow-out in indoor seawater tank of chonnam national university for 61 days (5. 29 - 7. 30. 2013). The cage models were made of polypropylene pipe for the frames. The size of the cage models were 920L×570B×500D ㎜, composed PE eel pots (hiding place: Ф130 ㎜×21 pieces, total volume: 0.51 m³) in TC, and 110L×800B×500D ㎜, constructed of PVC pipes (hiding place: Ф130 ㎜×21 pieces, total volume: 0.27 m³)in DC and UC(440L×460×620 ㎜ volume: 0.13 m³). In Experiment TC, DC and TC models were set up in the flow - through tank (7000L×6000B×820D ㎜, water depth: 0.82 m) at Chonnal National University, Yeosu, Korea for period of 99 days (17 July to 22 April 2013). In Experiment, a total 200 sea cucumbers were used, with an initial body wet weight of 64.68 ± 33.94 g. The water temperatures ranged from 10.88 to 16.65℃, and salinity ranged from 34.68 to 32.26 psu. Sea cucumbers were fed a mixed diet(mud, mineral, fish meal, etc.) and the feed was supplied to 3% of the weight. The results of an experiment (e.g. biological parameters) were analyzed with the statistical computer package SPSS ver. 21 from IBM. In Experiment, the survival rates of sea cucumber in UC (100%) and DC (87.62%) were higher than those of the TC (48.57%). The specific growth rate (SGR) of sea cucumber cages in DC (0.19%day<SUP>-1</SUP>) SGR was slightly higher than those UC (-0.25%day<SUP>-1</SUP>) results. The next step is to conduct indoor water tank test in order to investigate whether or not the rearing systems could be incorporated effectively for sea cucumber aquaculture industry. considering the season and the stocking densities of sea cucumber reared in the systems.
해상 실험에 의한 튜브형 양성기구의 밀도별 왜문어(Octopus vulgaris Cuvier)의 생존율 및 성장률
권인영(Inyeong Kwon),문선주(Sunju Moon),이효영(Hyoyeong Lee),박대혁(De-Hyuk Park),서준범(Jun-Beom Seo),신여림(Yeo-Rim Shin),김태호(Taeho Kim) 전남대학교 어업기술연구소 2015 어업기술연구소보고지 Vol.8 No.1
The purpose of this study is to evaluate the biological performances, survival and growth rates of the common octopus in individual rearing systems according to stocking density. The experiments were performed near Yeosu, Korea in the water depth (5-7 m) were used with an initial body wet weight of 486 ± 114 g. The cage models were made of polypropylene tube for the frames. The size of the cage models were 1100L × 460B × 500D mm, The three cages were used. The installation of eight each 1200L × 200D mm (density 10kg/㎥), 700L × 160D mm (density 30kg/㎥) and 350L × 130D mm (density 60kg/㎥) tube inside the cage. At this site, the water temperature ranged from 12.25 to 21.36℃, and salinity ranged from 30 to 32.79 psu, DO ranged from 5.08 to 9.28 mg/L, pH ranged from 7.8 to 8.13, respectively. Octopuses were fed a diet of frozen sardine (Sardinops melanostictus) and swimming crab (Portunus trituberculatus) (approximately 3-8% of their body weights). The results of the experiments were analysed with the statistical computer package SPSS ver. 21 from IBM. In experiment, the survival rates of octopus in density 10 kg/㎥ tube(75%) and 30 kg/㎥ tube(87.5%) were higher than those of density 60 kg/m3 tube(62.5%). The specific growth rate (SGR) of octopus in density 60 kg/m3 tube(0.24%/d-1) was slightly higher than those of density 10 kg/m3 tube(0.18%d<SUP>-1</SUP>) and 30 kg/m3 tube(0.17%d<SUP>-1</SUP>).
실내 수조에서 수온의 변화에 따른 아무르불가사리(Asterias amurensis)와 별불가사리(Asterina pectinifera)의 행동 패턴
권인영(Inyeong Kwon),신형식(Hyung-Sik Shin),장주영(Ju-Yeong Jang),홍석규(Suk-Kyu Hong),김태호(Taeho Kim) 전남대학교 어업기술연구소 2014 어업기술연구소보고지 Vol.7 No.1
A series of experiments which observed the movement patterns of starfishs (Asterias amurensis and Asterina pectinifera.) upon changes of water temperature (10, 15, 20 and 25°C) were carried out in an indoor water tank. In the experiment, the moving speed and distance of starfishs (Asterias amurensis and Asterina pectinifera) in the water tank was analyzed based through observations of video camera footage and PVS software. A total of 24 starfishs were used, with an initial body wet weight of 79.86±20.12 g (Asterias amurensis), 66.50±38.03 g (Asterina pectinifera) respetively. The movement patterns of starfishs (Asterias amurensis and Asterina pectinifera.) were observed using video camera for 12 h a day. Asterias amurensis showed the highest activity at 15℃ of water temperature. The maximum moving distance and speed of Asterias amurensis were about 2.64 m and approximately 0.0040 ㎧ at 15℃, respectively. The minimum observed moving distance and speed of Asterias amurensis were about 0.40 m at 25℃ and approximately 0.0012 ㎧ at 25℃, respectively. On the other hand, Asterina pectinifera showed the highest activity at 25℃ of water temperature. The maximum moving distance and speed of Asterina pectinifera were about 3.18 m and approximately 0.0049 ㎧ at 25°C, respectively. The minimum observed moving distance and speed of Asterina pectinifera were about 0.55 m at 15℃ and approximately 0.0018 ㎧ at 10℃, respectively. It was observed that the optimum water temperature for starfishs (Asterias amurensis and Asterina pectinifera) movement was about Asterias amurensis in 15°C and Asterina pectinifera in 25℃, respectively.
통발의 그물코 크기 변화에 따른 낙지의 입망 행동과 어획 성능
권인영 ( Inyeong Kwon ),김태호 ( Taeho Kim ) 한국수산해양기술학회 2021 수산해양기술연구 Vol.57 No.3
This study was conducted to evaluate the performance of the octopus pot according to mesh sizes. Entering behavior of Octopus minor and bait (Macrophthalmus japonicus) escape rate on the mesh sizes of the pots were investigated for six times in indoor tank. The sea trials for evaluating the performance of Octopus minor pot to different mesh sizes (22, 20 and 18 mm) were conducted for six times from 2017 to 2018 in the coastal sea of Deukyang Bay, the Republic of Korea. Behavior patterns of contact pot to leaved pot were more frequent than contact pot to bait search. When the octopus contacted to the pot, there was no clear search behavior to distinguish the mesh sizes. Total catch of 46% was accounted for 18 mm pots, followed by 34% at 20 mm and 20% at 22 mm (P < 0.05). Catch per unit effort was calculated as 30 g/pot at 22 mm, 44 g/pot at 20 mm and 59 g/pot at 18 mm. As a result of evaluating 50% selection of mantle length and weight on the mesh sizes, mantle length (mm) and weight (g) were 84.6 and 147.8 in 22 mm, followed by 20 and 18 mm.
실내수조에서 문어 (Octopus vulgaris)의 수온변화 및 은신처 유무에 따른 이동거리와 평균속력
권인영(Inyeong KWON),문선주(Sunju MOON),이대덕(Dae-Duk LEE),박호준(Ho-Jun PARK),김태호(Taeho KIM) 전남대학교 어업기술연구소 2016 어업기술연구소보고지 Vol.9 No.1
The common octopus (Octopus vulgaris) is considered a strong candidate for marine aquaculture due to its high growth rate and competitive market price. To investigate product potential, This study aims to investigate the moving patterns of common octopus between pipe and no shelter type up on changes of water temperature (10, 15 and 20℃). Experiments were conducted in a recirculation aquaculture system with dimensions of 2100 × 1000 × 1100 mm for 15 days (16 to 30 Nov, 2015). Three octopuses used in the experiment was 430 ± 21.6 g in body weight. Video cameras were set on the upper, front and inside of water tank and video camera footage were analyzed by PVS (private virtual studio) program. The octopuses showed the highest activity at 20℃ of water temperature in no shelter condition and at 15℃ of water temperature in shelter condition. The maximum moving distance and speed of 29.80 m in no shelter condition and approximately 27 10-3m/s at 20℃.
실내 수조에서 먹이 지급 및 미지급에 따른 왜문어(Octopus vulgaris)의 행동반응
권인영(Inyeong Kwon),남윤상(Yun-Sang Nam),박정인(Joeng-In Park),정철호(Chul-Ho Jeong),김태호(Taeho Kim) 전남대학교 어업기술연구소 2014 어업기술연구소보고지 Vol.7 No.1
The common octopus (octopus vulgaris) is considered very potential candidates for marine aquaculture diversity, as they show fast growth and retain high market price. The purpose of this study is to evaluate behavioral responses of the octopus vulgaris on feed and no feed conditions in indoor water tank for 24 days (9.17. ~ 10.10., 2013). In the experiment, swimming speed, activity aggression and accessibility of octopus in the water tank was analyzed based through observations of video camera footage and PVS software. A total of 6 octopuses were used, with an initial body weight of 241.6 ± 48.4 g and the water temperatures ranged from 15 to 16℃, and salinity raged 33 psu. All of the octopuses showed a higher growth rate, activity aggression and accessibility in feed condition rather than no feed conditions. In particularly, growth rate of octopuses decreased to 1.21% in 2-3days later in no feed condition. The swimming speed was reduced from 0.32 ㎧ in feed condition to 0.10 ㎧ in no feed condition. Activity of octopus vulgaris reduced nearly four times from an average of 115 in feed condition to 32 in no feed condition respectively.
해상에서 VIE 추적 조사에 의한 양성 기구의 수용 밀도 및 개체 크기별 해삼 (Apostichopus japonicus, Selenka)의 성장 비교
문선주 ( Sunju Moon ),권인영 ( Inyeong Kwon ),김태호 ( Taeho Kim ) 한국어업기술학회 2017 수산해양기술연구 Vol.53 No.1
The objective of this study was to investigate the growth rate and the optimal stocking density of sea cucumbers. Grow-out was studied in situ by conducting a follow-up survey using visible implant elastomer (VIE) tags. The rearing systems were made of polypropylene pipe for the frames and netting. The experiment ran for 70 days near Yeosu, Korea in the water depth of about 7 m. A total of 576 sea cucumbers which have three groups of body sizes (small: 5.15, medium: 12.34 and large: 23.26 g) were used. The five groups of stocking densities (150, 300, 450, 600 and 850 g/m<sup>2</sup>) in rearing system for sea cucumber were considered. Sea cucumbers were fed a mixed diet (mud, mineral, fish meal, etc.). The feed was supplied to 10% of their body wet weight once every 7 days. The survival rate (73%) of sea cucumber in 850 g/m<sup>2</sup> was lower than those of other density groups (150 g/m<sup>2</sup>: 89%, 300 g/m<sup>2</sup>: 84%, 450 g/m<sup>2</sup>: 78% and 600 g/m<sup>2</sup>: 86%). The survival rate of medium size group was higher than those of small and large groups regardless of the density (P< 0.05). Most of density groups have no significant difference except for 850 g/m<sup>2</sup> (P >0.05). The growth rate of small size group (0.63% day<sup>-1</sup>) was higher than those of medium (0.38% day<sup>-1</sup>) and large (0.34% day<sup>-1</sup>) group regardless of the density (P<0.05). The threshold water temperature was 11.0℃ for sea cucumber growth in winter season.
LC-MS/MS를 이용한 조피볼락 근육에서 구충제 febantel 및 그 대사체들의 정량분석을 위한 시료 전처리 방법의 비교 분석
임재웅 ( Jae-woong Lim ),권인영 ( Inyeong Kwon ),김태호 ( Taeho Kim ),김위식 ( Wi-sik Kim ),강소영 ( So Young Kang ) 한국어병학회 2021 한국어병학회지 Vol.34 No.2
This study presents the evaluation of sample extraction and purification procedure for the determination of residues of febantel and its metabolites, fenbendazole, oxfendazole and oxfendazole sulfone in rockfish (Sebastes schlegeli) muscle using liquid chromatography-tandem mass spectrometry. Residues of febantel and its metabolites in rockfish muscle were analyzed using each different sample preparation method from Korean Food Standards Codex (KFSC), Food Safety and Inspection Service (FSIS, USA), and the modified FSIS method using QuEChERS kit (FSIS-Q), respectively. Each method was compared for mean recoveries and repeatabilities. Since FSIS-Q showed higher repeatabilities (coefficient of variation, CV of 2.4%~10.9%) than those of FSIS method (CV of 4.6%~17.5%), recoveries from FSIS-Q were compared with those from KFSC method. FSIS-Q showed significantly higher recoveries of 83.1%~110.1% (P < 0.05) than those from KFSC method of 64.7%~107.4%. In addition, FSIS-Q showed a good linearity over the range of 2.5~200 μg/kg, and excellent sensitivities with limit of detection of 0.46~0.71 μg/kg and limit of quantification of 1.08~2.15 μg/kg. Although all the sample preparation methods turned out to be able to meet CODEX guideline for all the compounds, FSIS method and FSIS-Q validated in this study could be applied to screening and quantification for residues of febantel and its metabolites in rockfish muscle with efficient preparation procedures.