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J. S. Kong(공정식),K. Min(민경현),J. Kim(김종민),J. Kim(김지수),G. Gao(가오그),H. Park(박효신),H. H. Han(한현호),D.-W. Cho(조동우) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월
To treat peripheral nerve deficits, a variety of synthetic and decellularized materials with anisotropic architectures are developed due to the advantages proposed by aligned structures with respect to cell activity, migration, and directionality. Additionally, narrowing microscale topological cue has advantages on alignment and migration of cells. However, up to date, fabricating 3D hydrogel constructs composed of thin microfibers under 50 μm using mechanically weak hydrogel like low concentrated dECM bioink is challenge, unlike synthetic polymer-based fabrication. In this study, we created a microgel printing bath solution to three-dimensionally print a dECM bioink, derived from porcine nerve, as a thin microfiber. A 30 μm filament resolution of low viscosity dECM hydrogel with a neutral pH was achieved using an alginate microgel supplemented printing bath. To treat the sciatic nerve defect model of rats, 3D printed cylindrical constructs retaining bundle of dECM microfibers were used as a filler of the polycaprolacton (PCL) conduit. The total number of regenerated axons and relative gastrocnemius muscle weight ratio were comparable to those of the autologous nerve graft group. Meanwhile, the results were superior to those of the porcine decellularized nerve tissue group or the 3D printed filler only group.
Development of Vascularized Outer Blood-retinal-barrier Model via 3D Cell Printing
J. Kim(김종민),J. S. Kong(공정식),J. Y. Won(원재연),D-.W. Cho(조동우) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월
The outer blood-retinal-barrier (oBRB) is formed by tight junction of retinal pigment epithelium (RPE) and controls the molecular transportation between choroid and retina. Moreover, the RPE plays a multifunctional role in ocular system, including clearance, visual and maintenance functions. Due to their crucial role, the loss of this epithelium causes a wide range of the disease related to retinal degeneration such as age-related macular degeneration. Unfortunately, currently, there is no cure for most of these diseases since there is no suitable testing platform. To overcome this limitation, we developed the 3D printed vascularized oBRB model. For this objective, we developed Bruch’s membrane derived bioink (BM-ECM), containing natural extracellular matrix (ECM). Using BM-ECM and 3D printing technology, the Bruch’s membrane mimetic substrate (BMMS) was developed to recapitulate the structural and ECM micro environment for RPE. Then, HUVEC and RPE cells were printed in both side of BMMS to from vascularized oBRB and confirmed their superiorities.